Resistin Enhances VCAM-1 Expression and Monocyte Adhesion in Human Osteoarthritis Synovial Fibroblasts by Inhibiting MiR-381 Expression through the PKC, p38, and JNK Signaling Pathways

The development of osteoarthritis (OA) is characterized by synovial inflammation and the upregulation of vascular cell adhesion molecule type 1 (VCAM-1) in human osteoarthritis synovial fibroblasts (OASFs). This increase in VCAM-1 expression promotes monocyte adhesion to OASFs. The adipokine resistin is known to promote the release of inflammatory cytokines during OA progression. In this study, we identified significantly higher levels of resistin and CD68 (a monocyte surface marker) expression in human OA tissue compared with in healthy control tissue. We also found that resistin enhances VCAM-1 expression in human OASFs and facilitates the adhesion of monocytes to OASFs. These effects were attenuated by inhibitors of PKCα, p38, and JNK; their respective siRNAs; and by a microRNA-381 (miR-381) mimic. In our anterior cruciate ligament transection (ACLT) rat model of OA, the inhibition of resistin activity prevented ACLT-induced damage to the OA rat cartilage and pathological changes in resistin and monocyte expression. We also found that resistin affects VCAM-1 expression and monocyte adhesion in human OASFs by inhibiting miR-381 synthesis via the PKCα, p38, and JNK signaling pathways. Our clarification of the crucial role played by resistin in the pathogenesis of OA may lead to more effective therapy that reduces OA inflammation.

Download Full-text

Single-Leg Jump Performance Before and After Exercise in Healthy and Anterior Cruciate Ligament Reconstructed Individuals

Journal of Sport Rehabilitation ◽

10.1123/jsr.2019-0159 ◽

2020 ◽

Vol 29 (7) ◽

pp. 879-885

Author(s):

Haley Bookbinder ◽

Lindsay V. Slater ◽

Austin Simpson ◽

Jay Hertel ◽

Joseph M. Hart

Keyword(s):

Anterior Cruciate Ligament ◽

Repeated Measures ◽

Contact Time ◽

Cruciate Ligament ◽

Ground Contact ◽

Jump Height ◽

Before And After ◽

Healthy Control ◽

Ground Contact Time ◽

Anterior Cruciate

Context: Many clinicians measure lower-extremity symmetry after anterior cruciate ligament reconstruction (ACLR); however, testing is completed in a rested state rather than postexercise. Testing postexercise may better model conditions under which injury occurs. Objective: To compare changes in single-leg performance in healthy and individuals with history of ACLR before and after exercise. Design: Repeated-measures case-control. Setting: Laboratory. Patients: Fifty-two subjects (25 control and 27 ACLR). Intervention: Thirty minutes of exercise. Main Outcome Measures: Limb symmetry and involved limb performance (nondominant for healthy) for single-leg hop, ground contact time, and jump height during the 4-jump test. Cohen d effect sizes were calculated for all differences identified using a repeated-measures analysis of variance. Results: Healthy controls hopped farther than ACLR before (d = 0.65; confidence interval [CI], 0.09 to 1.20) and after exercise (d = 0.60; CI, 0.04 to 1.15). Those with ACLR had longer ground contact time on the reconstructed limb compared with the uninvolved limb after exercise (d = 0.53; CI, −0.02 to 1.09), and the reconstructed limb had greater ground contact time compared with the healthy control limb after exercise (d = 0.38; CI, −0.21 to 0.73). ACLR were less symmetrical than healthy before (d = 0.38; CI, 0.17 to 0.93) and after exercise (d = 0.84; CI, 0.28 to 1.41), and the reconstructed limb demonstrated decreased jump height compared with the healthy control limbs before (d = 0.75; CI, 0.19 to 1.31) and after exercise (d = 0.79; CI, 0.23 to 1.36). Conclusions: ACLR became more symmetric, which may be from adaptations of the reconstructed limb after exercise. Changes in performance and symmetry may provide additional information regarding adaptations to exercise after reconstruction.

Download Full-text

Visfatin Promotes IL-6 and TNF-α Production in Human Synovial Fibroblasts by Repressing miR-199a-5p through ERK, p38 and JNK Signaling Pathways

International Journal of Molecular Sciences ◽

10.3390/ijms19010190 ◽

2018 ◽

Vol 19 (1) ◽

pp. 190 ◽

Cited By ~ 22

Author(s):

Min-Huan Wu ◽

Chun-Hao Tsai ◽

Yuan-Li Huang ◽

Yi-Chin Fong ◽

Chih-Hsin Tang

Keyword(s):

Signaling Pathways ◽

Synovial Fibroblasts ◽

Jnk Signaling ◽

Tnf Α

Download Full-text

Side-to-side differences in anterior cruciate ligament volume in healthy control subjects

Journal of Biomechanics ◽

10.1016/j.jbiomech.2009.10.006 ◽

2010 ◽

Vol 43 (3) ◽

pp. 576-578 ◽

Cited By ~ 13

Author(s):

S.T. Jamison ◽

D.C. Flanigan ◽

H.N. Nagaraja ◽

A.M.W. Chaudhari

Keyword(s):

Anterior Cruciate Ligament ◽

Cruciate Ligament ◽

Control Subjects ◽

Healthy Control ◽

Anterior Cruciate

Download Full-text

Meniscal Suture Influence on Driving Ability 6 Weeks after Anterior Cruciate Ligament Reconstruction with Hamstring Autograft

The Journal of Knee Surgery ◽

10.1055/s-0041-1729553 ◽

2021 ◽

Author(s):

Maria Perez-Mozas ◽

Jesus Payo-Ollero ◽

Veronica Montiel ◽

Juan Ramon Valenti-Nin ◽

Andres Valenti-Azcarate

Keyword(s):

Anterior Cruciate Ligament ◽

Cruciate Ligament ◽

Weight Bearing ◽

Driving Performance ◽

Driving Ability ◽

Control Group ◽

Hamstring Autograft ◽

Meniscal Suture ◽

Healthy Control ◽

Anterior Cruciate

AbstractThe purpose of this study was to determine if driving ability 6 weeks after anterior cruciate ligament (ACL) reconstruction is affected by the addition of a meniscal suture. It was also hypothesized that no differences in the driving performance would be found between right or left knee surgery subgroups. A total of 82 people participated in this prospective cohort study: 36 healthy controls, 26 patients undergoing isolated ACL (iACL) reconstruction with hamstring autograft, and 20 patients undergoing ACL and meniscal suture (ACL-MS) reconstruction. ACL-MS group followed a weight-bearing and movement restriction protocol during the first 2 postoperative weeks, whereas patients undergoing iACL could start range-of-motion exercises and full weight-bearing ambulation on the first postoperative day. A driving simulator that reproduced real-life driving conditions was used to evaluate driving ability. The software analyzed multiple driving and braking variables. Driving performance in the sixth postoperative week was compared with that of a healthy control group. Subgroup analysis considering additional procedures (iACL, ACL-MS) and the side of the operated knee (right, left) was also performed. No statistically significant differences were found in the demographic characteristics nor in the driving performance (collisions, p = 0.897; sidewalk invasions, p = 0.749; pedestrian impact, p = 0.983) between iACL, ACL-MS, and control groups. No statistically significant differences were found in right–left subgroup analysis. The results of the present study show that patients in their sixth postoperative week after right or left ACL reconstruction showed similar driving performance as compared with a healthy control group, regardless of associating or not a meniscal suture, suggesting it is safe to resume driving 6 weeks after the mentioned surgeries.

Download Full-text

The effect of cell-based therapy with autologous synovial fibroblasts activated by exogenous TGF-β1 on the in situ frozen-thawed anterior cruciate ligament

Journal of Orthopaedic Science ◽

10.1007/s00776-004-0810-7 ◽

2004 ◽

Vol 9 (5) ◽

pp. 488-494 ◽

Cited By ~ 16

Author(s):

Tomoo Okuizumi ◽

Harukazu Tohyama ◽

Eiji Kondo ◽

Kazunori Yasuda

Keyword(s):

Anterior Cruciate Ligament ◽

Cruciate Ligament ◽

Synovial Fibroblasts ◽

Cell Based Therapy ◽

Anterior Cruciate ◽

Tgf Β1

Download Full-text

A failed repair of a type 1 anterior cruciate ligament tear: Case presentation

Case Reports and Images in Surgery ◽

10.15761/cris.1000148 ◽

2020 ◽

Vol 3 (2) ◽

Author(s):

Courtney A Quinn ◽

Anthony Ignozzi ◽

Mark D Miller

Keyword(s):

Anterior Cruciate Ligament ◽

Cruciate Ligament ◽

Anterior Cruciate Ligament Tear ◽

Case Presentation ◽

Ligament Tear ◽

Anterior Cruciate

Download Full-text

Ramp Lesion Subtypes: Prevalence, Imaging, and Arthroscopic Findings in 2156 Anterior Cruciate Ligament Reconstructions

The American Journal of Sports Medicine ◽

10.1177/03635465211006103 ◽

2021 ◽

pp. 036354652110061

Author(s):

Mathieu Thaunat ◽

Pramod Ingale ◽

Alexandre Penet ◽

Samih Kacem ◽

Ibrahim Haidar ◽

...

Keyword(s):

Anterior Cruciate Ligament ◽

Pivot Shift ◽

Cruciate Ligament ◽

Lesion Type ◽

Ramp Lesion ◽

Mri Scans ◽

Anterior Cruciate ◽

Type 3

Background: Ramp lesions are defined as a particular type of injury within the posterior horn of the medial meniscus and its meniscocapsular attachments. Five subtypes have been described: type 1, meniscocapsular lesion; type 2, partial superior lesion; type 3, partial inferior lesion or hidden type; type 4, complete tear in the red zone; and type 5, complete double tear. Purpose: (1) To determine the prevalence of different subtypes of ramp lesions in patients undergoing arthroscopic anterior cruciate ligament reconstruction (ACLR). (2) To describe the characteristics of ramp lesions based on imaging and diagnostic arthroscopy. Study Design: Cross-sectional study; Level of evidence, 3. Methods: All patients who underwent arthroscopic ACLRs between November 2015 and November 2018 by 2 senior surgeons at 1 institution were evaluated retrospectively (1) to describe the subtypes of ramp lesions diagnosed intraoperatively using transnotch arthroscopic exploration of the posteromedial compartment and (2) to look for any factors significantly associated with these subtypes. The following parameters were studied: demographics; history and clinical findings including time between injury and surgery, side-to-side laxity, and pivot shift; lesions missed on magnetic resonance imaging (MRI) scans and medial proximal tibial bone contusion visible on MRI scans; and arthroscopic confirmation of ramp lesion (ie, prevalence), associated lateral meniscal tear, or medial chondral tear. Results: Out of 2156 primary or revision arthroscopic reconstructions, 334 ramp lesions were confirmed, giving a prevalence of 15.5%. The subtype distribution was as follows: type 1, 47.9%; type 2, 4.8%; type 3, 11.4%; type 4, 28.7%; type 5, 7.2%. Multivariate analysis showed that gross pivot shift was significantly associated with complete ramp tears (odds ratio, 4.8; 95% CI, 1.7-17.2). Hidden lesions (type 3, inferior partial tear in the red zone) were the most likely to be missed on preoperative MRI (45.9%). Conclusion: In a population undergoing ACLR, the prevalence of ramp lesions was 15.5%. Among the subtypes of ramp lesion, the most common was a meniscocapsular junction tear (type 1). Partial inferior tears (type 3) were the most likely to be missed on preoperative MRI scans. Gross pivot shift was significantly associated with complete ramp tears (types 1, 4, and 5).

Download Full-text

Drop-Landing Performance and Knee-Extension Strength After Anterior Cruciate Ligament Reconstruction

Journal of Athletic Training ◽

10.4085/1062-6050-50.2.11 ◽

2015 ◽

Vol 50 (6) ◽

pp. 596-602 ◽

Cited By ~ 16

Author(s):

Christopher M. Kuenze ◽

Nathaniel Foot ◽

Susan A. Saliba ◽

Joseph M. Hart

Keyword(s):

Body Mass Index ◽

Anterior Cruciate Ligament ◽

Anterior Cruciate Ligament Reconstruction ◽

Ligament Reconstruction ◽

Cruciate Ligament ◽

Knee Extension ◽

Cruciate Ligament Reconstruction ◽

Knee Extension Strength ◽

Healthy Control ◽

Anterior Cruciate

Context Individuals with a history of anterior cruciate ligament reconstruction (ACLR) are at greater risk of reinjury and developing early-onset osteoarthritis due to persistent abnormal joint loading. Real-time clinical assessment tools may help identify patients experiencing abnormal movement patterns after ACLR. Objective To compare performance on the Landing Error Scoring System (LESS) between participants with ACLR and uninjured control participants and to determine the relationship between LESS score and knee-extension strength in these participants. Design Controlled laboratory study. Setting Research laboratory. Patients or Other Participants Forty-six recreationally active participants, consisting of 22 with ACLR (12 men, 10 women; age = 22.5 ± 5.0 years, height = 172.8 ± 7.2 cm, mass = 74.2 ± 15.6 kg, body mass index = 24.6 ± 4.0) and 24 healthy control participants (12 men, 12 women; age = 21.7 ± 3.6 years, height = 168.0 ± 8.8 cm, mass = 69.2 ± 13.6 kg, body mass index = 24.3 ± 3.2) were enrolled. Main Outcome Measure(s) Bilateral normalized knee-extension maximal voluntary isometric contraction (MVIC) torque (Nm/kg) and LESS scores were measured during a single testing session. We compared LESS scores between groups using a Mann-Whitney U test and the relationships between LESS scores and normalized knee-extension MVIC torque using Spearman ρ bivariate correlations. Results The ACLR participants had a greater number of LESS errors (6.0 ± 3.6) than healthy control participants (2.8 ± 2.2; t44 = −3.73, P = .002). In ACLR participants, lower normalized knee-extension MVIC torque in the injured limb (ρ = −0.455, P = .03) was associated with a greater number of landing errors. Conclusions Participants with ACLR displayed more errors while landing. The occurrence of landing errors was negatively correlated with knee-extension strength, suggesting that weaker participants had more landing errors. Persistent quadriceps weakness commonly associated with ACLR may be related to a reduced quality of lower extremity movement during dynamic tasks.

Download Full-text

Landing Evaluation in Soccer Players with or without Anterior Cruciate Ligament Reconstruction

International Journal of Sports Medicine ◽

10.1055/a-1171-1900 ◽

2020 ◽

Vol 41 (13) ◽

pp. 962-971

Author(s):

Ahmad Alanazi ◽

Katy Mitchell ◽

Toni Roddey ◽

Aqeel Alenazi ◽

Msaad Alzhrani ◽

...

Keyword(s):

Knee Flexion ◽

Cruciate Ligament ◽

Knee Extension ◽

Soccer Players ◽

Control Group ◽

Landing Biomechanics ◽

Healthy Control ◽

Anterior Cruciate ◽

Hip Flexion ◽

Main Effects

AbstractThe purpose of this study was to evaluate landing biomechanics in soccer players following ACLR during two landing tasks. Eighteen soccer players with an ACLR and 18 sex-matched healthy control soccer players participated in the study. Planned landing included jumping forward and landing on the force-plates, whereas unplanned landing included jumping forward to head a soccer ball and landing on the force-plates. A significant landing×group interaction was found only for knee flexion angles (p=0.002). Follow-up comparisons showed that the ACL group landed with greater knee flexion during planned landing compared with unplanned landing (p<0.001). Significant main effects of landing were found. The unplanned landing showed reduction in hip flexion (p<0.001), hip extension moments (p<0.013), knee extension moments (p<0.001), and peak pressure (p<0.001). A significant main effect for group for gastrocnemius muscle was found showing that the ACL group landed with reduced gastrocnemius activity (p=0.002). Unplanned landing showed greater injury predisposing factors compared with planned landing. The ACL group showed nearly similar landing biomechanics to the control group during both landing tasks. However, the ACL group used a protective landing strategy by reducing gastrocnemius activity.

Download Full-text