scholarly journals Effects of Cryogenic Storage on Human Amnion Epithelial Cells

Cells ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 1696 ◽  
Author(s):  
Raghuraman C. Srinivasan ◽  
Stephen C. Strom ◽  
Roberto Gramignoli
Keyword(s):  
Epithelial Cell ◽  
Epithelial Cells ◽  
Hematopoietic Cells ◽  
Full Term ◽  
Human Amnion ◽  
Cellular Therapies ◽  
Multipotent Cells ◽  
Number Of Patients ◽  
Promising Source ◽  
Amnion Epithelial Cells

Perinatal stem cells and epithelial cells isolated from full term amnion membrane, in particular, have attracted interest over the last decade, as a promising source of multipotent cells for cellular therapies. Human amnion epithelial cells (hAEC) have been used to treat monogenetic liver disease such as maple syrup urine disease or fibrosis of the liver in preclinical studies. In most studies xeno-transplants of hAEC were conducted without providing immunosuppression to recipients, reflecting the tolerogenic properties of hAEC. For many cell types, successful cryopreservation is critical for providing a readily available, off-the-shelf product. In this study, hAEC were isolated from full-term human placenta from 14 different donors, cryopreserved using a protocol and reagents commonly adopted for epithelial cell preservation. The cells were analyzed in terms of survival, recovery, and homogeneity, profiled for surface markers characteristic of epithelial, mesenchymal, endothelial, or hematopoietic cells. There were no significant differences observed in the percentage of cells with epithelial cell markers before and after cryopreservation. The relative proportion of stromal and hematopoietic cells was significantly reduced in hAEC preparations after cryopreservation. The expression of stem cell and immunomodulatory molecules were confirmed in the final product. Since multipotent cells are readily available from full-term placenta, this novel cell source might significantly increase the number of patients eligible to receive cellular therapies for liver and other diseases.

scholarly journals Intermediate filament cytoskeleton of amnion epithelium and cultured amnion epithelial cells: expression of epidermal cytokeratins in cells of a simple epithelium.

1985 ◽  
Vol 100 (4) ◽  
pp. 997-1009 ◽  
Author(s):  
S Regauer ◽  
W W Franke ◽  
I Virtanen
Keyword(s):  
Epithelial Cell ◽  
Epithelial Cells ◽  
General Concept ◽  
Cytoskeletal Proteins ◽  
Full Term ◽  
Two Dimensional Gel Electrophoresis ◽  
Stratified Epithelium ◽  
Epithelial Cell Lines ◽  
Amnion Epithelial Cells

Using immunofluorescence microscopy and two-dimensional gel electrophoresis, we compared the cytoskeletal proteins expressed by human amnion epithelium in situ, obtained from pregnancies of from 10-wk to birth, with the corresponding proteins from cultured amnion epithelial cells and cultures of cells from the amniotic fluid of 16 week pregnancies. Epithelia of week 16 fetuses already display tissue-specific patterns of cytokeratin polypeptides which are similar, although not identical, to those of the corresponding adult tissues. In the case of the simple amnion epithelium, a complex and characteristic complement of cytokeratin polypeptides of Mr 58,000 (No. 5), 56,000 (No. 6), 54,000 (No. 7), 52,500 (No. 8), 50,000 (No. 14), 46,000 (No. 17), 45,000 (No. 18), and 40,000 (No. 19) is present by week 10 of pregnancy and is essentially maintained until birth, with the addition of cytokeratin No. 4 (Mr 59,000) and the disappearance of No. 7 (Mr 54,000) at week 16 of pregnancy. In full-term placentae, the amnion epithelium displays two morphologically distinct regions, i.e., a simple and a stratified epithelium, both of which express the typical amnion cytokeratin polypeptides. However, in addition the stratified epithelium also synthesizes large amounts of special epidermal cytokeratins such as No. 1 (Mr 68,000), 10 (Mr 56,500), and 11 (Mr 56,000). In culture amnion epithelial cells obtained from either 16-wk pregnancies or full-term placentae will continue to synthesize the amnion-typical cytokeratin pattern, except for a loss of detection of component No. 4. This pattern is considerably different from the cytokeratins synthesized by cultures of cells from amniotic fluids (cytokeratins No. 7, 8, 18, and 19, sometimes with trace amounts of No. 17) and from several so-called "amnion epithelial cell lines." In addition, amnion epithelial cells in situ as well as amnion epithelial cell cultures appear to be heterogeneous in that they possess some cells that co-express cytokeratins and vimentin. These observations lead to several important conclusions: In contrast to the general concept of recent literature, positively charged cytokeratins of the group No. 4-6 can be synthesized in a simple, i.e., one-layered epithelium. The change from simple to stratified amnion epithelium does not require a cessation of synthesis of cytokeratins of the simple epithelium type, but in this case keratins characteristic of the terminally differentiated epidermis (No. 1, 10, and 11) are also synthesized.(ABSTRACT TRUNCATED AT 400 WORDS)

scholarly journals Interleukin 4 Production by Human Amnion Epithelial Cells and Regulation of its Activity by Glycosaminoglycan Binding1

1995 ◽  
Vol 52 (4) ◽  
pp. 839-847 ◽  
Author(s):  
Catherine A. Jones ◽  
Keryn A. Williams ◽  
John J. Finlay-Jones ◽  
Prue H. Hart
Keyword(s):  
Epithelial Cells ◽  
Interleukin 4 ◽  
Human Amnion ◽  
Amnion Epithelial Cells

scholarly journals Transplantation of Human Amnion Epithelial Cells Reduces Hepatic Fibrosis in Immunocompetent CCl4-Treated Mice

2010 ◽  
Vol 19 (9) ◽  
pp. 1157-1168 ◽  
Author(s):  
Ursula Manuelpillai ◽  
Jorge Tchongue ◽  
Dinushka Lourensz ◽  
Vijesh Vaghjiani ◽  
Chrishan S. Samuel ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Hepatic Fibrosis ◽  
Human Amnion ◽  
Amnion Epithelial Cells

scholarly journals Effect of Human Amnion Epithelial Cells on the Acute Inflammatory Response in Fetal Sheep

2017 ◽  
Vol 8 ◽  
Author(s):  
Alana Westover ◽  
Jacqueline M. Melville ◽  
Courtney McDonald ◽  
Rebecca Lim ◽  
Graham Jenkin ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Inflammatory Response ◽  
Acute Inflammatory Response ◽  
Fetal Sheep ◽  
Human Amnion ◽  
Amnion Epithelial Cells

Transplantation of Human Amnion Epithelial Cells Improves Endometrial Regeneration in Rat Model of Intrauterine Adhesions

2020 ◽  
Vol 29 (20) ◽  
pp. 1346-1362
Author(s):  
Xiaolan Ouyang ◽  
Shuang You ◽  
Yulin Zhang ◽  
Chanyu Zhang ◽  
Guanghui Zhang ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Rat Model ◽  
Intrauterine Adhesions ◽  
Human Amnion ◽  
Amnion Epithelial Cells ◽  
Endometrial Regeneration

Adhesion of human amnion epithelial cells to extracellular matrix

1984 ◽  
Vol 153 (2) ◽  
pp. 425-438 ◽  
Author(s):  
J.D. Aplin ◽  
S. Campbell ◽  
L.J. Foden
Keyword(s):  
Extracellular Matrix ◽  
Epithelial Cells ◽  
Human Amnion ◽  
Amnion Epithelial Cells

scholarly journals Human Amnion Epithelial Cells Produce Soluble Factors that Enhance Liver Repair by Reducing Fibrosis While Maintaining Regeneration in a Model of Chronic Liver Injury

2020 ◽  
Vol 29 ◽  
pp. 096368972095022
Author(s):  
Alexander Hodge ◽  
Neil Andrewartha ◽  
Dinushka Lourensz ◽  
Robyn Strauss ◽  
Jeanne Correia ◽  
...  
Keyword(s):  
Liver Injury ◽  
Epithelial Cells ◽  
Chronic Liver ◽  
Hepatocyte Proliferation ◽  
Chronic Liver Injury ◽  
Paracrine Factors ◽  
Soluble Factors ◽  
Human Amnion ◽  
Liver Repair ◽  
Amnion Epithelial Cells

Human amnion epithelial cells (hAECs) exert potent antifibrotic and anti-inflammatory effects when transplanted into preclinical models of tissue fibrosis. These effects are mediated in part via the secretion of soluble factors by hAECs which modulate signaling pathways and affect cell types involved in inflammation and fibrosis. Based on these reports, we hypothesized that these soluble factors may also support liver regeneration during chronic liver injury. To test this, we characterized the effect of both hAECs and hAEC-conditioned medium (CM) on liver repair in a mouse model of carbon tetrachloride (CCl4)-induced fibrosis. Liver repair was assessed by liver fibrosis, hepatocyte proliferation, and the liver progenitor cell (LPC) response. We found that the administration of hAECs or hAEC-CM reduced liver injury and fibrosis, sustained hepatocyte proliferation, and reduced LPC numbers during chronic liver injury. Additionally, we undertook in vitro studies to document both the cell–cell and paracrine-mediated effects of hAECs on LPCs by investigating the effects of co-culturing the LPCs and hAECs and hAEC-CM on LPCs. We found little change in LPCs co-cultured with hAECs. In contrast, hAEC-CM enhances LPC proliferation and differentiation. These findings suggest that paracrine factors secreted by hAECs enhance liver repair by reducing fibrosis while promoting regeneration during chronic liver injury.

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