scholarly journals Effect of a Lens Protein in Low-Temperature Culture of Novel Immortalized Human Lens Epithelial Cells (iHLEC-NY2)

Cells ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 2670
Author(s):  
Naoki Yamamoto ◽  
Shun Takeda ◽  
Natsuko Hatsusaka ◽  
Noriko Hiramatsu ◽  
Noriaki Nagai ◽  
...  

The prevalence of nuclear cataracts was observed to be significantly higher among residents of tropical and subtropical regions compared to those of temperate and subarctic regions. We hypothesized that elevated environmental temperatures may pose a risk of nuclear cataract development. The results of our in silico simulation revealed that in temperate and tropical regions, the human lens temperature ranges from 35.0 °C to 37.5 °C depending on the environmental temperature. The medium temperature changes during the replacement regularly in the cell culture experiment were carefully monitored using a sensor connected to a thermometer and showed a decrease of 1.9 °C, 3.0 °C, 1.7 °C, and 0.1 °C, after 5 min when setting the temperature of the heat plate device at 35.0 °C, 37.5 °C, 40.0 °C, and 42.5 °C, respectively. In the newly created immortalized human lens epithelial cell line clone NY2 (iHLEC-NY2), the amounts of RNA synthesis of αA crystallin, protein expression, and amyloid β (Aβ)1-40 secreted into the medium were increased at the culture temperature of 37.5 °C compared to 35.0 °C. In short-term culture experiments, the secretion of Aβ1-40 observed in cataracts was increased at 37.5 °C compared to 35.0 °C, suggesting that the long-term exposure to a high-temperature environment may increase the risk of cataracts.

2015 ◽  
pp. 1-10 ◽  
Author(s):  
Marlyn P. Langford ◽  
Thomas B. Redens ◽  
Chanping Liang ◽  
A. Scott Kavanaugh ◽  
Donald E. Texada

2006 ◽  
Vol 32 (3) ◽  
pp. 510-514 ◽  
Author(s):  
Juergen Kampmeier ◽  
Alicja Baldysiak-Figiel ◽  
Yvonne de Jong-Hesse ◽  
Gerhard K. Lang ◽  
Gabriele E. Lang

1999 ◽  
Vol 249 (1) ◽  
pp. 123-130 ◽  
Author(s):  
YiQun Wang ◽  
HaiYing He ◽  
J.Samuel Zigler ◽  
Takeshi Iwata ◽  
Nobuhiro Ibaraki ◽  
...  

ACS Omega ◽  
2020 ◽  
Vol 5 (50) ◽  
pp. 32171-32182
Author(s):  
Xiaojuan Chen ◽  
Pengfei Li ◽  
Guowei Zhang ◽  
Lihua Kang ◽  
Bai Qin ◽  
...  

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