scholarly journals From Zygote to Blastocyst: Application of Ultrashort Lasers in the Field of Assisted Reproduction and Developmental Biology

Diagnostics ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1897
Author(s):  
Inna Ilina ◽  
Dmitry Sitnikov
Keyword(s):  
Developmental Biology ◽  
Laser Pulses ◽  
Embryonic Cell ◽  
Ultrashort Laser Pulses ◽  
Assisted Hatching ◽  
Vitro Fertilization ◽  
Fundamental Research ◽  
Laser Sources ◽  
Ultrashort Laser

Although the use of lasers in medical diagnosis and therapies, as well as in fundamental biomedical research is now almost routine, advanced laser sources and new laser-based methods continue to emerge. Due to the unique ability of ultrashort laser pulses to deposit energy into a microscopic volume in the bulk of a transparent material without disrupting the surrounding tissues, the ultrashort laser-based microsurgery of cells and subcellular components within structurally complex and fragile specimens such as embryos is becoming an important tool in developmental biology and reproductive medicine. In this review, we discuss the mechanisms of ultrashort laser pulse interaction with the matter, advantages of their application for oocyte and preimplantation embryo microsurgery (e.g., for oocyte/blastomere enucleation and embryonic cell fusion), as well as for nonlinear optical microscopy for studying the dynamics of embryonic development and embryo quality assessment. Moreover, we focus on ultrashort laser-based approaches and techniques that are increasingly being applied in the fundamental research and have the potential for successful translation into the IVF (in vitro fertilization) clinics, such as laser-mediated individual embryo labelling and controlled laser-assisted hatching.

Welding characteristics of foturan glass using ultrashort laser pulses

2012 ◽  
Author(s):  
Isamu Miyamoto ◽  
Kristian Cvecek ◽  
Yasuhiro Okamoto ◽  
Michael Schmidt ◽  
Henry Helvajian
Keyword(s):  
Laser Pulses ◽  
Ultrashort Laser Pulses ◽  
Ultrashort Laser

Evaluation of Chip-Breakers Manufactured by Ultrashort Laser Pulses in Cemented Carbide Tools

2017 ◽  
Author(s):  
Marcelo Bertolete Carneiro ◽  
Patrícia Alves Barbosa ◽  
Ricardo Samad ◽  
NIlson Vieira ◽  
Wagner de Rossi ◽  
...  
Keyword(s):  
Laser Pulses ◽  
Ultrashort Laser Pulses ◽  
Cemented Carbide ◽  
Cemented Carbide Tools ◽  
Ultrashort Laser

Picosecond Single-Photon and Femtosecond Two-Photon Pulsed Laser Stimulation for IC Characterization and Failure Analysis

2009 ◽  
Author(s):  
V. Pouget ◽  
E. Faraud ◽  
K. Shao ◽  
S. Jonathas ◽  
D. Horain ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Single Photon ◽  
Pulsed Laser ◽  
Laser Pulses ◽  
Femtosecond Laser Pulses ◽  
Ultrashort Laser Pulses ◽  
Laser Stimulation ◽  
Two Photon ◽  
Resolution Improvement ◽  
Ultrashort Laser

Abstract This paper presents the use of pulsed laser stimulation with picosecond and femtosecond laser pulses. We first discuss the resolution improvement that can be expected when using ultrashort laser pulses. Two case studies are then presented to illustrate the possibilities of the pulsed laser photoelectric stimulation in picosecond single-photon and femtosecond two-photon modes.

scholarly journals Super-Heavy Ions Acceleration Driven by Ultrashort Laser Pulses at Ultrahigh Intensity

2021 ◽  
Vol 11 (2) ◽  
Author(s):  
Pengjie Wang ◽  
Zheng Gong ◽  
Seong Geun Lee ◽  
Yinren Shou ◽  
Yixing Geng ◽  
...  
Keyword(s):  
Heavy Ions ◽  
Laser Pulses ◽  
Ultrashort Laser Pulses ◽  
Ultrashort Laser

scholarly journals Analytical model for the depth progress of percussion drilling with ultrashort laser pulses

2021 ◽  
Vol 127 (5) ◽  
Author(s):  
Daniel Holder ◽  
Rudolf Weber ◽  
Thomas Graf ◽  
Volkher Onuseit ◽  
David Brinkmeier ◽  
...  
Keyword(s):  
Analytical Model ◽  
Experimental Validation ◽  
Laser Pulses ◽  
Ultrashort Laser Pulses ◽  
Picosecond Pulses ◽  
Hole Depth ◽  
Laser Percussion Drilling ◽  
Pulse Energies ◽  
Ultrashort Laser ◽  
Percussion Drilling

AbstractA simplified analytical model is presented that predicts the depth progress during and the final hole depth obtained by laser percussion drilling in metals with ultrashort laser pulses. The model is based on the assumption that drilled microholes exhibit a conical shape and that the absorbed fluence linearly increases with the depth of the hole. The depth progress is calculated recursively based on the depth changes induced by the successive pulses. The experimental validation confirms the model and its assumptions for percussion drilling in stainless steel with picosecond pulses and different pulse energies.

scholarly journals High-quality percussion drilling with ultrashort laser pulses

2021 ◽  
Vol 127 (9) ◽  
Author(s):  
A. Feuer ◽  
R. Weber ◽  
R. Feuer ◽  
D. Brinkmeier ◽  
T. Graf
Keyword(s):  
Stainless Steel ◽  
Laser Fluence ◽  
Ablation Threshold ◽  
Laser Pulses ◽  
Ultrashort Laser Pulses ◽  
High Quality ◽  
Edge Quality ◽  
Ultrashort Laser ◽  
Percussion Drilling

AbstractThe influence of the laser fluence on the quality of percussion-drilled holes was investigated both experimentally and by an analytical model. The study reveals that the edge quality of the drilled microholes depends on the laser fluence reaching the rear exit of the hole and changes with the number of pulses applied after breakthrough. The minimum fluence that must reach the hole’s exit in order to obtain high-quality microholes in stainless steel was experimentally found to be 2.8 times the ablation threshold.

scholarly journals High-quality net shape geometries from additively manufactured parts using closed-loop controlled ablation with ultrashort laser pulses

2020 ◽  
Vol 9 (1-2) ◽  
pp. 101-110 ◽  
Author(s):  
Daniel Holder ◽  
Artur Leis ◽  
Matthias Buser ◽  
Rudolf Weber ◽  
Thomas Graf
Keyword(s):  
Surface Roughness ◽  
Closed Loop ◽  
High Surface ◽  
Laser Pulses ◽  
Ultrashort Laser Pulses ◽  
Minimum Thickness ◽  
High Quality ◽  
Surface Optical ◽  
The Individual ◽  
Ultrashort Laser

AbstractAdditively manufactured parts typically deviate to some extent from the targeted net shape and exhibit high surface roughness due to the size of the powder grains that determines the minimum thickness of the individual slices and due to partially molten powder grains adhering on the surface. Optical coherence tomography (OCT)-based measurements and closed-loop controlled ablation with ultrashort laser pulses were utilized for the precise positioning of the LPBF-generated aluminum parts and for post-processing by selective laser ablation of the excessive material. As a result, high-quality net shape geometries were achieved with surface roughness, and deviation from the targeted net shape geometry reduced by 67% and 63%, respectively.

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