scholarly journals Luciferase-Based Detection of Antibodies for the Diagnosis of HPV-Associated Head and Neck Squamous Cell Carcinoma

Diagnostics ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 89
Author(s):  
Peter D. Burbelo ◽  
Adrija Chaturvedi ◽  
Abner L. Notkins ◽  
Sreenivasulu Gunti
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Point Of Care ◽  
Neck Squamous Cell Carcinoma ◽  
Wild Type ◽  
Hpv 16 ◽  
Wild Type P53 ◽  
Neodymium Magnets

Point-of-care tests are needed for the screening of head and neck squamous cell carcinoma (HNSCC) and other malignancies. Luciferase immunoprecipitation systems (LIPS), employing light-emitting proteins, were used to examine serum antibodies against several cancer-associated targets in blood donor controls and subjects with colon cancer (CC) and HNSCC. The assessment of antibodies against the wild type p53 tumor antigen showed that approximately 25% of the CC and 20% of the HNSCC patients were seropositive. In addition, humoral responses against two p53 mutants, p53-R175H and p53-R273H, generally tracked the antibody responses seen against wild type p53. Analysis of antibodies against highly specific biomarkers of HPV-16-associated malignancy, E2, E6, and E7 oncoproteins, revealed no seropositivity in blood donors and CC patients. However, 45% (9/20) of the HNSCC patients showed E6 seropositivity, which overlapped all the detectable E2 (40%; 8/20) and E7 seropositive subjects (35%; 7/20). Using neodymium magnets, ultrarapid LIPSTICKS testing of HPV-16 E6 antibodies in <60 s per HNSCC sample demonstrated almost the same diagnostic performance (40% sensitivity and 100% specificity) as LIPS testing in 2.5 h. While additional improvements and standardization are needed, these results highlight the possibility of using these approaches for the diagnosis of HPV-16-associated HNSCC.

scholarly journals Prevalence of Human Papillomavirus Associated with Head and Neck Squamous Cell Carcinoma in Jordanian Patients

2020 ◽  
Vol 14 (1) ◽  
pp. 57-64
Author(s):  
Ashraf I. Khasawneh ◽  
Nisreen Himsawi ◽  
Jumana Abu-Raideh ◽  
Muna Salameh ◽  
Niveen Abdullah ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Human Papillomavirus ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Hpv Infection ◽  
Neck Squamous Cell Carcinoma ◽  
Hpv 16 ◽  
Hpv Dna ◽  
Prophylactic Measures

Background: In addition to smoking and alcohol consumption, human papillomavirus (HPV) is a leading etiology for Head and Neck Squamous Cell Carcinoma (HNSCC). However, this causal association is still understudied in Middle Eastern populations. Objective: The aim of this study was to determine the prevalence of HPV-associated infection in the Jordanian HNSCC patients and the associated HPV genotypes. Methods: Formalin-Fixed Paraffin-Embedded (FFPE) squamous cell carcinoma samples of the head and neck were collected from two referral centers in Amman, Jordan to determine the existence of HPV DNA. After DNA extraction HPV infection and genotyping were identified using real-time PCR. Results: HPV DNA was detected in 19 out of 61 (31.1%) HNSCC samples. Despite screening for 28 different genotypes, HPV 16 was the only genotype identified in all examined samples. Most HPV-positive samples were obtained from the oropharynx (41.7%), oral cavity (37%), and larynx (18.2%). No significant association between HPV 16 genotype and age, sex, tobacco use, anatomical location, or tumor grade was noticed. Conclusion: This study reported a high association between HPV 16 genotype and HNSCC in Jordanian patients. These data should facilitate the implementation of appropriate HPV awareness campaigns, and activate selective prophylactic measures against HPV infection.

scholarly journals Frequency of HPV-16 in Saliva and Blood in Head and Neck Squamous Cell Carcinoma in Pakistani Population

2020 ◽  
Vol 29 (04) ◽  
pp. 228-234
Author(s):  
Saima Saher ◽  
◽  
Sofia Ali Syed ◽  
Asifa Iqbal ◽  
Faryal Ali Syed ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Human Papilloma Virus ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Neck Squamous Cell Carcinoma ◽  
Hpv 16 ◽  
Papilloma Virus ◽  
Pakistani Population ◽  
Clinicopathological Variables

OBJECTIVE: Pakistan is geographically located in the region where prevalence of head and neck squamous cell carcinoma (HNSCC) is high. Human papilloma virus (HPV) subtype-16 plays significant role in heterogeneity of HNSCC. The objective of this study was to evaluate the frequency of HPV-16 in both blood and saliva in HNSCC in our population and its association with clinicopathological features. METHODOLOGY: A total of 200 samples comprising 100 blood and 100 saliva samples were collected. Hundred samples (saliva; n=50, blood; n=50) were collected from histopathologically confirmed head and neck squamous cell carcinoma patients and 100 samples (saliva; n=50 saliva, blood; n=50) were collected from healthy individuals without a history of cancer as controls. HPV-16 was detected using conventional polymerase chain reaction and gel-electrophoresis. The frequency and distribution of clinicopathological variables were calculated in percentages. Chi-square test was used to analyze the association of HPV-16 positivity with clinicopathological variables. RESULTS: Of 100 samples from HNSCC patients, 11 (11%) samples were positive for HPV-16. Of 11 HPV-16 positive samples, 8 (16%) were detected in saliva (n=50) and 3 (6%) were detected in plasma (n=50). Of 100 samples from control group, both blood and saliva samples were negative for HPV-16. No statistically significant association was found between HPV-16 in saliva and blood and clinicopathological characteristics (P > 0.05). CONCLUSION: Within the limit of current study, HPV-16 has a low-frequency in saliva and blood in HNSCC in our population. Further studies on HNSCC with large sample and HPV-subtypes are suggested. KEYWORD: Blood; human papilloma virus; saliva; squamous cell carcinoma. HOW TO CITE: Saher S, Syed SA, Iqbal A, Syed FA, Amin F, Sultan A, Anees M. Frequency of HPV-16 in saliva and blood in head and neck squamous cell carcinoma in Pakistani population. J Pak Dent Assoc 2020;29(4):228-234.

DNA methylation regulated microRNAs in HPV-16-induced head and neck squamous cell carcinoma (HNSCC)

2018 ◽  
Vol 448 (1-2) ◽  
pp. 321-333 ◽  
Author(s):  
M. K. Sannigrahi ◽  
Rajni Sharma ◽  
Varinder Singh ◽  
Naresh K. Panda ◽  
Vidya Rattan ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Neck Squamous Cell Carcinoma ◽  
Hpv 16
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