scholarly journals Effect of Salt Addition upon the Production of Metabolic Compounds by Yarrowia lipolytica Cultivated on Biodiesel-Derived Glycerol Diluted with Olive-Mill Wastewaters

Energies ◽  
2019 ◽  
Vol 12 (19) ◽  
pp. 3649 ◽  
Author(s):  
Markella Tzirita ◽  
Maria Kremmyda ◽  
Dimitris Sarris ◽  
Apostolis A. Koutinas ◽  
Seraphim Papanikolaou
Keyword(s):  
Citric Acid ◽  
Yarrowia Lipolytica ◽  
Crude Glycerol ◽  
Value Added ◽  
Total Phenolic ◽  
Industrial Biotechnology ◽  
Phenol Removal ◽  
Biological Liquid ◽  
The Impact ◽  
Olive Mill

One of the major environmental problems is the highly toxic agro-industrial waste called olive mill wastewater (OMW), deriving from olive oil production. On the other hand, the continuous development of the biological liquid fuel industry (biodiesel and bioethanol) makes it mandatory the process and exploitation of their main by-products, crude glycerol. This study dealt with the biotechnological conversions of biodiesel-derived crude glycerol with the use of the non-conventional yeast Yarrowia lipolytica in media that had been diluted with OMWs. OMWs, employed as simultaneous liquid medium and substrate, is a new trend recently appearing in Industrial Biotechnology, where value-added metabolites could be produced with simultaneous partial detoxification (i.e. decolorization and phenol removal) of the used residue. In the present study, diluted OMWs (containing 2.0 g/L of total phenolic compounds) blended with 70.0 g/L crude glycerol were employed as substrates. Production of value-added compounds by Y. lipolytica strain ACA-YC 5031 was studied in nitrogen-limited media favoring the production of secondary metabolites (i.e. citric acid, polyols, microbial lipids, polysaccharides). Batch-flask cultures were carried out and the impact of the addition of different NaCl concentrations (1.0%, 3.0%, 5.0% w/w) added upon the biochemical behavior of the strain was studied. Remarkable biomass production was observed in all trials, while in the “blank” experiment (no OMWs and no salt added), the metabolism was shifted toward the synthesis of polyols (Σpolyols = mannitol + arabitol + erythritol > 20 g/L and maximum total citric acid-Cit (sum of citric and isocitric acid) = 10.5 g/L). Addition of OMWs resulted in Citmax = 32.7 g/L, while Σpolyols concentration dropped to <15 g/L. Addition of salt in the OMW-based media slightly reduced the produced biomass, while Cit production drastically increased, reaching a final value of 54.0 g/L (conversion yield of Cit produced per unit of glycerol consumed = 0.82 g/g) in the trial with addition of 5.0% NaCl. Finally, significant color and phenols removal were observed, evaluating the yeast as a decontamination medium for the OMW and a great candidate for the production of value-added compounds.

scholarly journals Production of Added-Value Chemical Compounds through Bioconversions of Olive-Mill Wastewaters Blended with Crude Glycerol by a Yarrowia lipolytica Strain

Molecules ◽  
2019 ◽  
Vol 24 (2) ◽  
pp. 222 ◽  
Author(s):  
Dimitris Sarris ◽  
Anna Rapti ◽  
Nikolaos Papafotis ◽  
Apostolis A. Koutinas ◽  
Seraphim Papanikolaou
Keyword(s):  
Citric Acid ◽  
Yarrowia Lipolytica ◽  
Acid Production ◽  
Crude Glycerol ◽  
Alcoholic Beverage ◽  
Biodiesel Production ◽  
Glycerol Concentration ◽  
Olive Mill Wastewaters ◽  
Lipolytica Strain ◽  
Olive Mill

Olive mill wastewaters (OMW) are the major effluent deriving from olive oil production and are considered as one of the most challenging agro-industrial wastes to treat. Crude glycerol is the main by-product of alcoholic beverage and oleochemical production activities including biodiesel production. The tremendous quantities of glycerol produced worldwide represent a serious environmental challenge. The aim of this study was to assess the ability of Yarrowia lipolytica strain ACA-DC 5029 to grow on nitrogen-limited submerged shake-flask cultures, in crude glycerol and OMW blends as well as in media with high initial glycerol concentration and produce biomass, cellular lipids, citric acid and polyols. The rationale of using such blends was the dilution of concentrated glycerol by OMW to (partially or fully) replace process tap water with a wastewater stream. The strain presented satisfactory growth in blends; citric acid production was not affected by OMW addition (Citmax~37.0 g/L, YCit/Glol~0.55 g/g) and microbial oil accumulation raised proportionally to OMW addition (Lmax~2.0 g/L, YL/X~20% w/w). Partial removal of color (~30%) and phenolic compounds (~10% w/w) of the blended media occurred. In media with high glycerol concentration, a shift towards erythritol production was noted (Erymax~66.0 g/L, YEry/Glol~0.39 g/g) simultaneously with high amounts of produced citric acid (Citmax~79.0 g/L, YCit/Glol~0.46 g/g). Fatty acid analysis of microbial lipids demonstrated that OMW addition in blended media and in excess carbon media with high glycerol concentration favored oleic acid production.

Valorization of Crude Glycerol into Citric Acid and Malic Acid by Yarrowia lipolytica

2020 ◽  
Vol 59 (39) ◽  
pp. 17165-17172
Author(s):  
Xiujuan Qian ◽  
Ning Xu ◽  
Yiwen Jing ◽  
Meng Song ◽  
Jie Zhou ◽  
...  
Keyword(s):  
Citric Acid ◽  
Yarrowia Lipolytica ◽  
Malic Acid ◽  
Crude Glycerol

Pretreatment of olive mill wastewater for citric acid production by Yarrowia lipolytica

2016 ◽  
Vol 33 ◽  
pp. S116-S117
Author(s):  
Seda Karasu-Yalcin ◽  
Kubra Eryasar ◽  
Buse Guler ◽  
Meryem Özdemir ◽  
Serife Baggul
Keyword(s):  
Citric Acid ◽  
Yarrowia Lipolytica ◽  
Acid Production ◽  
Olive Mill Wastewater ◽  
Citric Acid Production ◽  
Olive Mill

scholarly journals Bioconversions of Biodiesel-Derived Glycerol into Sugar Alcohols by Newly Isolated Wild-Type Yarrowia lipolytica Strains

Reactions ◽  
2021 ◽  
Vol 2 (4) ◽  
pp. 499-513
Author(s):  
Eleni-Stavroula Vastaroucha ◽  
Sofia Maina ◽  
Savvoula Michou ◽  
Ourania Kalantzi ◽  
Chrysanthi Pateraki ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Crude Glycerol ◽  
Ph Value ◽  
Negative Impact ◽  
Value Added ◽  
Biodiesel Production ◽  
Glycerol Concentration ◽  
Conversion Yield ◽  
Ph Values ◽  
Mannitol Production

The utilization of crude glycerol, generated as a by-product from the biodiesel production process, for the production of high value-added products represents an opportunity to overcome the negative impact of low glycerol prices in the biodiesel industry. In this study, the biochemical behavior of Yarrowia lipolytica strains FMCC Y-74 and FMCC Y-75 was investigated using glycerol as a carbon source. Initially, the effect of pH value (3.0–7.0) was examined to produce polyols, intracellular lipids, and polysaccharides. At low pH values (initial pH 3.0–5.0), significant mannitol production was recorded. The highest mannitol production (19.64 g L−1) was obtained by Y. lipolytica FMCC Y-74 at pH = 3.0. At pH values ranging between 5.0 and 6.0, intracellular polysaccharides synthesis was favored, while polyols production was suppressed. Subsequently, the effect of crude glycerol and its concentration on polyols production was studied. Y. lipolytica FMCC Y-74 showed high tolerance to impurities of crude glycerol. Initial substrate concentrations influence polyols production and distribution with a metabolic shift toward erythritol production being observed when the initial glycerol concentration (Gly0) increased. The highest total polyols production (=56.64 g L−1) was obtained at Gly0 adjusted to ≈120 g L−1. The highest polyols conversion yield (0.59 g g−1) and productivity (4.36 g L−1 d−1) were reached at Gly0 = 80 g L−1. In fed-batch intermittent fermentation with glycerol concentration remaining ≤60 g L−1, the metabolism was shifted toward mannitol biosynthesis, which was the main polyol produced in significant quantities (=36.84 g L−1) with a corresponding conversion yield of 0.51 g g−1.

Sync between leucine, biotin and citric acid to improve lipid production by Yarrowia lipolytica on crude glycerol-based media

2020 ◽  
Vol 142 ◽  
pp. 105764 ◽  
Author(s):  
Sara Magdouli ◽  
Tayssir Guedri ◽  
Tarek Rouissi ◽  
Satinder Kaur Brar ◽  
Jean-Francois Blais
Keyword(s):  
Citric Acid ◽  
Yarrowia Lipolytica ◽  
Crude Glycerol ◽  
Lipid Production

Valorization of Crude Glycerol, Residue Deriving from Biodiesel- Production Process, with the Use of Wild-type New Isolated Yarrowia lipolytica Strains: Production of Metabolites with Pharmaceutical and Biotechnological Interest

2019 ◽  
Vol 20 (10) ◽  
pp. 881-894 ◽  
Author(s):  
Dimitris Sarris ◽  
Zoe Sampani ◽  
Anna Rapti ◽  
Seraphim Papanikolaou
Keyword(s):  
Citric Acid ◽  
Yarrowia Lipolytica ◽  
Crude Glycerol ◽  
Fetal Brain ◽  
Biodiesel Production ◽  
Added Value ◽  
Substrate Uptake ◽  
Glycerol Concentration ◽  
Dry Cell Weight ◽  
Lipolytica Strain

Background & Objective:Crude glycerol (Glol), used as substrate for screening eleven natural Yarrowia lipolytica strains in shake-flask experiments. Aim of this study was to assess the ability of the screened strains to produce biomass (dry cell weight; X), lipid (L), citric acid (Cit), mannitol (Man), arabitol (Ara) and erythritol (Ery), compounds presenting pharmaceutical and biotechnological interest, in glycerol-based nitrogen-limited media, in which initial glycerol concentration had been adjusted to 40 g/L.Methods:Citric acid may find use in biomedical engineering (i.e. drug delivery, tissue engineering, bioimaging, orthopedics, medical device coating, wound dressings). Polyols are considered as compounds with non-cariogenic and less calorigenic properties as also with low insulin-mediated response. Microbial lipids containing polyunsaturated fatty acids (PUFA) are medically and dietetically important (selective pharmaceutical and anticancer properties, aid fetal brain development, the sight function of the eye, hormonal balance and the cardio-vascular system, prevent reasons leading to type-2 diabetes, present healing and anti-inflammatory effects).Results:All strains presented satisfactory microbial growth (Xmax=5.34-6.26 g/L) and almost complete substrate uptake. The principal metabolic product was citric acid (Citmax=8.5-31.7 g/L). Production of cellular lipid reached the values of 0.33-0.84 g/L. Polyols were also synthesized as strain dependent compounds (Manmax=2.8-6.1 g/L, Aramax ~2.0 g/L, Erymax= 0.5-3.8 g/L). The selected Y. lipolytica strain ACA-DC 5029 presented satisfactory growth along with synthesis of citric acid and polyols, thus, was further grown on media presenting an increased concentration of Glol~75 g/L. Biomass, lipid and citric acid production presented significant enhancement (Xmax=11.80 g/L, Lmax=1.26 g/L, Citmax=30.8 g/L), but conversion yield of citric acid produced per glycerol consumed was decreased compared to screening trials. Erythritol secretion (Erymax=15.6 g/L) was highly favored, suggesting a shift of yeast metabolism from citric acid accumulation towards erythritol production. Maximum endopolysaccharides (IPS) concentration was 4.04 g/L with yield in dry weight 34.2 % w/w.Conclusion:Y. lipolytica strain ACA-YC 5029 can be considered as a satisfactory candidate grown in high concentrations of crude glycerol to produce added-value compounds that interest pharmaceutical and biotechnology industries.

Citric acid, biomass and cellular lipid production by Yarrowia lipolytica strains cultivated on olive mill wastewater-based media

2011 ◽  
Vol 86 (11) ◽  
pp. 1439-1448 ◽  
Author(s):  
Dimitris Sarris ◽  
Maria Galiotou-Panayotou ◽  
Apostolis A. Koutinas ◽  
Michael Komaitis ◽  
Seraphim Papanikolaou
Keyword(s):  
Citric Acid ◽  
Yarrowia Lipolytica ◽  
Lipid Production ◽  
Olive Mill Wastewater ◽  
Cellular Lipid ◽  
Olive Mill

scholarly journals Developing an Olive Biorefinery in Slovenia: Analysis of Phenolic Compounds Found in Olive Mill Pomace and Wastewater

Molecules ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 7
Author(s):  
Ana Miklavčič Višnjevec ◽  
Paul Baker ◽  
Adam Charlton ◽  
Dave Preskett ◽  
Kelly Peeters ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Compound ◽  
Phenolic Content ◽  
Seasonal Effects ◽  
Total Phenolic ◽  
Array Detector ◽  
Economic Modelling ◽  
Olive Pomace ◽  
The Impact ◽  
Olive Mill

The valorization of olive pomace through the extraction of phenolic compounds at an industrial scale is influenced by several factors that can have a significant impact on the feasibility of this approach. These include the types and levels of phenolic compounds that are present, the impact that seasonal variation and cultivar type have on the phenolic compound content in both olive pomace and mill effluents and the technological approach used to process the olive crop. Chemical analysis of phenolic compounds was performed using an HPLC-diode-array detector (DAD)-qTOF system, resulting in the identification of 45 compounds in olive mill wastewater and pomace, where secoiridoids comprised 50–60% of the total phenolic content. This study examined three different factors that could impact the phenolic compound content of these processing streams, including cultivar types typically grown on local farms in Slovenia, the type of downstream processing used and seasonality effects. Olive crop varieties sourced from local farms showed high variability, and the highest phenolic content was associated with the local variety “Istrska Belica”. During processing, the phenolic content was on average approximately 50% higher during two-phase decanting compared to three-phase decanting and the type of compound present significantly different. An investigation into the seasonal effects revealed that the phenolic content was 20% higher during the 2019 growing season compared to 2018. A larger sample size over additional growing seasons is required to fully understand the annual variation in phenolic compound content. The methods and results used in this study provide a basis for further analysis of phenolic compounds present in the European Union’s olive crop processing residues and will inform techno-economic modelling for the development of olive biorefineries in Slovenia.

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