Production of Itaconic Acid from Cellulose Pulp: Feedstock Feasibility and Process Strategies for an Efficient Microbial Performance

This study assessed the feasibility of using bleached cellulose pulp from Eucalyptus wood as a feedstock for the production of itaconic acid by fermentation. Additionally, different process strategies were tested with the aim of selecting suitable conditions for an efficient production of itaconic acid by the fungus Aspergillus terreus. The feasibility of using cellulose pulp was demonstrated through assays that revealed the preference of the strain in using glucose as carbon source instead of xylose, mannose, sucrose or glycerol. Additionally, the cellulose pulp was easily digested by enzymes without requiring a previous step of pretreatment, producing a glucose-rich hydrolysate with a very low level of inhibitor compounds, suitable for use as a fermentation medium. Fermentation assays revealed that the technique used for sterilization of the hydrolysate (membrane filtration or autoclaving) had an important effect in its composition, especially on the nitrogen content, consequently affecting the fermentation performance. The carbon-to-nitrogen ratio (C:N ratio), initial glucose concentration and oxygen availability, were also important variables affecting the performance of the strain to produce itaconic acid from cellulose pulp hydrolysate. By selecting appropriate process conditions (sterilization by membrane filtration, medium supplementation with 3 g/L (NH4)2SO4, 60 g/L of initial glucose concentration, and oxygen availability of 7.33 (volume of air/volume of medium)), the production of itaconic acid was maximized resulting in a yield of 0.62 g/g glucose consumed, and productivity of 0.52 g/L·h.

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Engineering efficient production of itaconic acid from diverse substrates in Escherichia coli

Journal of Biotechnology ◽

10.1016/j.jbiotec.2017.03.026 ◽

2017 ◽

Vol 249 ◽

pp. 73-81 ◽

Cited By ~ 8

Author(s):

Peiching Chang ◽

Grey S. Chen ◽

Hsiang-Yuan Chu ◽

Ken W. Lu ◽

Claire R. Shen

Keyword(s):

Escherichia Coli ◽

Itaconic Acid ◽

Efficient Production

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Reaction Kinetics of Levulinic Acid Synthesis from Glucose Using Bronsted Acid Catalyst

10.21203/rs.3.rs-609706/v1 ◽

2021 ◽

Author(s):

Meutia Ermina Toif ◽

Muslikhin Hidayat ◽

Rochmadi Rochmadi ◽

Arief Budiman

Keyword(s):

Reaction Kinetics ◽

Glucose Concentration ◽

Levulinic Acid ◽

Acid Catalyst ◽

Operating Conditions ◽

Bronsted Acid ◽

Brønsted Acid ◽

Initial Glucose Concentration ◽

Brönsted Acid ◽

Kinetics Of

Abstract Glucose is the primary derivative of lignocellulosic biomass, which is abundantly available. Glucose has excellent potential to be converted into valuable compounds such as ethanol, sorbitol, gluconic acid, and levulinic acid (LA). Levulinic acid is a very promising green platform chemical. It is composed of two functional groups, ketone and carboxylate groups which can act as highly reactive electrophiles for nucleophilic attack so it has extensive applications, including fuel additives, raw materials for the pharmaceutical industry, and cosmetics. The reaction kinetics of LA synthesis from glucose using hydrochloric acid catalyst (bronsted acid) were studied in a wide range of operating conditions, i.e., temperature of 140-180 oC, catalyst concentration of 0.5-1.5 M, and initial glucose concentration of 0.1-0.5 M. The highest LA yield is 48.34 %wt at 0.1 M initial glucose concentration, 1 M HCl, and temperature of 180 oC. The experimental results show that the bronsted acid catalyst's reaction pathway consists of glucose decomposition to levoglucosan (LG), conversion of LG to 5-hydroxymethylfurfural (HMF), and rehydration of HMF to LA. The experimental data yields a good fitting by assuming a first-order reaction model.

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Effect of Reaction Parameters on the Production of Ethyl Levulinate from Glucose in Ethanol

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.512-515.388 ◽

2012 ◽

Vol 512-515 ◽

pp. 388-391 ◽

Cited By ~ 1

Author(s):

Chun Chang ◽

Xiao Xian Jiang ◽

Ting Zhang ◽

Bo Li

Keyword(s):

Sulfuric Acid ◽

Acid Concentration ◽

Glucose Concentration ◽

Sulfuric Acid Concentration ◽

Ethanol Conversion ◽

Reaction Parameters ◽

Water Addition ◽

Initial Glucose Concentration ◽

Ethyl Levulinate ◽

Higher Temperature

In order to explore the forming rules of ethyl levulinate from glucose in ethanol, several reaction parameters including the initial glucose concentration (10~30 g/L), sulfuric acid concentration (0.1~2 wt%), temperature (170~200 °C) and water addition were investigate, respectively. In addition, effect of the acid concentration on ethanol conversion was also studied. The results show that lower initial glucose concentration is helpful to improve the ethyl levulinate yield, and higher acid concentration can improve the conversion of glucose to ethyl levulinate, which also can improve the condensation of the ethanol. However, higher temperature is unfavorable for the increase of ethyl levulinate yield, and the ethyl levulinate decreased significantly with the increase of water addition.

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The pH mediated effects of initial glucose concentration on the transitory occurrence of extracellular metabolites, gas exchange, and growth yields of aerobic batch cultures ofklebsiella pneumoniae

Biotechnology and Bioengineering ◽

10.1002/bit.260370713 ◽

1991 ◽

Vol 37 (7) ◽

pp. 696-696

Author(s):

J. J. L�nsmann Iversen

Keyword(s):

Gas Exchange ◽

Glucose Concentration ◽

Growth Yields ◽

Initial Glucose Concentration ◽

Batch Cultures ◽

Mediated Effects ◽

Extracellular Metabolites

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Monitoring Initial Glucose Concentration for Optimum pH Control during Fermentation of Microbial Cellulose in Rotary Discs Reactor

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.594-595.319 ◽

2013 ◽

Vol 594-595 ◽

pp. 319-324 ◽

Cited By ~ 3

Author(s):

Khairul Azly Zahan ◽

Norhayati Pa’e ◽

Kok Fook Seng ◽

Ida Idayu Muhamad

Keyword(s):

Glucose Concentration ◽

Ph Control ◽

Fermentation Medium ◽

Initial Glucose Concentration ◽

Cellulose Production ◽

Microbial Cellulose ◽

Cellulose Yield ◽

Optimum Ph ◽

Initial Ph ◽

Wet Weight

The study aimed to investigate the effect of initial glucose concentration on the microbial cellulose production using Acetobacter xylinum in a Rotary Discs Reactor (RDR-2 liter volume). The fermentations were carried out for four days at temperature 28°C, initial pH 6.5, and 9 rpm of rotation speed; meanwhile, the initial glucose concentration was manipulated in the range of 0.5-5.0 % (w/v). The cell growth was stimulated using 1.4% (v/v) ethanol in the fermentation medium. The result indicated that 1% (w/v) of initial glucose concentration provided the highest microbial cellulose yield with total wet weight of 296.1657g/l. The increase of initial glucose concentration resulted to the decrease of microbial cellulose yield and greater pH drop after fermentation. It can be conclude that production of microbial cellulose using RDR could produce relatively much higher microbial cellulose with less amounts of glucose in a shorter fermentation period compared to static fermentation due to more efficient oxygen uptake during rotary movements and homogenous environment for microbial growth.

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Reaction Kinetics of Levulinic Acid Synthesis from Glucose Using Bronsted Acid Catalyst

BULLETIN OF CHEMICAL REACTION ENGINEERING AND CATALYSIS ◽

10.9767/bcrec.16.4.12197.904-915 ◽

2021 ◽

Vol 16 (4) ◽

pp. 904-915

Author(s):

Meutia Ermina Toif ◽

Muslikhin Hidayat ◽

Rochmadi Rochmadi ◽

Arief Budiman

Keyword(s):

Reaction Kinetics ◽

Glucose Concentration ◽

Levulinic Acid ◽

Operating Conditions ◽

Nucleophilic Attack ◽

Bronsted Acid ◽

Brønsted Acid ◽

Initial Glucose Concentration ◽

Brönsted Acid ◽

Kinetics Of

Glucose is one of the primary derivative products from lignocellulosic biomass, which is abundantly available. Glucose has excellent potential to be converted into valuable compounds such as ethanol, sorbitol, gluconic acid, and levulinic acid (LA). Levulinic acid is an exceptionally promising green platform chemical. It comprises two functional groups, ketone and carboxylate, acting as highly reactive electrophiles for a nucleophilic attack. Therefore, it has extensive applications, including fuel additives, raw materials for the pharmaceutical industry, and cosmetics. This study reports the reaction kinetics of LA synthesis from glucose catalyzed by hydrochloric acid (HCl), a Bronsted acid, that was carried out under a wide range of operating conditions; i.e. the temperature of 140–180 °C, catalyst concentration of 0.5–1.5 M, and initial glucose concentration of 0.1–0.5 M. The highest LA yield of 48.34 % was able to be obtained from an initial glucose concentration of 0.1 M and by using 1 M HCl at 180 °C. The experimental results show that the Bronsted acid-catalyzed reaction pathway consists of glucose decomposition to levoglucosan (LG), conversion of LG to 5-hydroxymethylfurfural (HMF), and rehydration of HMF to LA. The experimental data yields a good fitting by assuming a first-order reaction model. Copyright © 2021 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0).

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Influence of pH and Initial Glucose Concentration on the Growth ofSaccharomyces cerevisiaeYeast Strain by Gravitational Field Flow Fractionation

Separation Science and Technology ◽

10.1080/01496395.2010.547240 ◽

2011 ◽

Vol 46 (6) ◽

pp. 893-903 ◽

Cited By ~ 2

Author(s):

G. Ch. Lainioti ◽

J. Kapolos ◽

A. Koliadima ◽

G. Karaiskakis

Keyword(s):

Gravitational Field ◽

Glucose Concentration ◽

Field Flow Fractionation ◽

Initial Glucose Concentration ◽

Flow Fractionation ◽

Influence Of Ph

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Pharmaceutical removal during managed aquifer recharge with pretreatment by advanced oxidation

Water Science & Technology Water Supply ◽

10.2166/ws.2012.050 ◽

2012 ◽

Vol 12 (6) ◽

pp. 755-767 ◽

Cited By ~ 15

Author(s):

K. Lekkerkerker-Teunissen ◽

E. T. Chekol ◽

S. K. Maeng ◽

K. Ghebremichael ◽

C. J. Houtman ◽

...

Keyword(s):

Membrane Filtration ◽

Advanced Oxidation Process ◽

Batch Reactor ◽

Advanced Oxidation ◽

Managed Aquifer Recharge ◽

Process Conditions ◽

Aquifer Recharge ◽

Anoxic Conditions ◽

Influent Water ◽

Removal Technologies

Organic micropollutants (OMPs) are detected in sources for drinking water and treatment possibilities are investigated. Innovative removal technologies are available such as membrane filtration and advanced oxidation, but also biological treatment should be considered. By combining an advanced oxidation process with managed aquifer recharge (MAR), two complementary processes are expected to provide a hybrid system for OMP removal, according to the multiple barrier approach. Laboratory scale batch reactor experiments were conducted to investigate the removal of dissolved organic carbon (DOC) and 14 different pharmaceutically active compounds (PhACs) from MAR influent water and water subjected to oxidation, under different process conditions. A DOC removal of 10% was found in water under oxic (aerobic) conditions for batch reactor experiments, a similar value for DOC removal was observed in the field. Batch reactor experiments for the removal of PhACs showed that the removal of pharmaceuticals ranged from negligible to more than 90%. Under oxic conditions, seven out of 14 pharmaceuticals were removed over 90% and 12 out of 14 pharmaceuticals were removed at more than 50% during 30 days of experiments. Under anoxic conditions, four out of 14 pharmaceuticals were removed over 90% and eight out of 14 pharmaceuticals were removed at more than 50% over 30 days' experiments. Carbamazepine and phenazone were persistent both under oxic and anoxic conditions. The PhACs removal efficiency with oxidized water was, for most compounds, comparable to the removal with MAR influent water.

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