scholarly journals An Automatic Method for Stomatal Pore Detection and Measurement in Microscope Images of Plant Leaf Based on a Convolutional Neural Network Model

Forests ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 954 ◽  
Author(s):  
Wenlong Song ◽  
Junyu Li ◽  
Kexin Li ◽  
Jingxu Chen ◽  
Jianping Huang

Stomata are microscopic pores on the plant epidermis that regulate the water content and CO2 levels in leaves. Thus, they play an important role in plant growth and development. Currently, most of the common methods for the measurement of pore anatomy parameters involve manual measurement or semi-automatic analysis technology, which makes it difficult to achieve high-throughput and automated processing. This paper presents a method for the automatic segmentation and parameter calculation of stomatal pores in microscope images of plant leaves based on deep convolutional neural networks. The proposed method uses a type of convolutional neural network model (Mask R-CNN (region-based convolutional neural network)) to obtain the contour coordinates of the pore regions in microscope images of leaves. The anatomy parameters of pores are then obtained by ellipse fitting technology, and the quantitative analysis of pore parameters is implemented. Stomatal microscope image datasets for black poplar leaves were obtained using a large depth-of-field microscope observation system, the VHX-2000, from Keyence Corporation. The images used in the training, validation, and test sets were taken randomly from the datasets (562, 188, and 188 images, respectively). After 10-fold cross validation, the 188 test images were found to contain an average of 2278 pores (pore widths smaller than 0.34 μm (1.65 pixels) were considered to be closed stomata), and an average of 2201 pores were detected by our network with a detection accuracy of 96.6%, and the intersection of union (IoU) of the pores was 0.82. The segmentation results of 2201 stomatal pores of black poplar leaves showed that the average measurement accuracies of the (a) pore length, (b) pore width, (c) area, (d) eccentricity, and (e) degree of stomatal opening, with a ratio of width-to-maximum length of a stomatal pore, were (a) 94.66%, (b) 93.54%, (c) 90.73%, (d) 99.09%, and (e) 92.95%, respectively. The proposed stomatal pore detection and measurement method based on the Mask R-CNN can automatically measure the anatomy parameters of pores in plants, thus helping researchers to obtain accurate stomatal pore information for leaves in an efficient and simple way.

2019 ◽  
Vol 24 (3) ◽  
pp. 220-228
Author(s):  
Gusti Alfahmi Anwar ◽  
Desti Riminarsih

Panthera merupakan genus dari keluarga kucing yang memiliki empat spesies popular yaitu, harimau, jaguar, macan tutul, singa. Singa memiliki warna keemasan dan tidak memilki motif, harimau memiliki motif loreng dengan garis-garis panjang, jaguar memiliki tubuh yang lebih besar dari pada macan tutul serta memiliki motif tutul yang lebih lebar, sedangkan macan tutul memiliki tubuh yang sedikit lebih ramping dari pada jaguar dan memiliki tutul yang tidak terlalu lebar. Pada penelitian ini dilakukan klasifikasi genus panther yaitu harimau, jaguar, macan tutul, dan singa menggunakan metode Convolutional Neural Network. Model Convolutional Neural Network yang digunakan memiliki 1 input layer, 5 convolution layer, dan 2 fully connected layer. Dataset yang digunakan berupa citra harimau, jaguar, macan tutul, dan singa. Data training terdiri dari 3840 citra, data validasi sebanyak 960 citra, dan data testing sebanyak 800 citra. Hasil akurasi dari pelatihan model untuk training yaitu 92,31% dan validasi yaitu 81,88%, pengujian model menggunakan dataset testing mendapatan hasil 68%. Hasil akurasi prediksi didapatkan dari nilai F1-Score pada pengujian didapatkan sebesar 78% untuk harimau, 70% untuk jaguar, 37% untuk macan tutul, 74% untuk singa. Macan tutul mendapatkan akurasi terendah dibandingkan 3 hewan lainnya tetapi lebih baik dibandingkan hasil penelitian sebelumnya.


2021 ◽  
Vol 1099 (1) ◽  
pp. 012001
Author(s):  
Srishti Garg ◽  
Tanishq Sehga ◽  
Aakriti Jain ◽  
Yash Garg ◽  
Preeti Nagrath ◽  
...  

2021 ◽  
Vol 16 ◽  
Author(s):  
Farida Alaaeldin Mostafa ◽  
Yasmine Mohamed Afify ◽  
Rasha Mohamed Ismail ◽  
Nagwa Lotfy Badr

Background: Protein sequence analysis helps in the prediction of protein functions. As the number of proteins increases, it gives the bioinformaticians a challenge to analyze and study the similarity between them. Most of the existing protein analysis methods use Support Vector Machine. Deep learning did not receive much attention regarding protein analysis as it is noted that little work focused on studying the protein diseases classification. Objective: The contribution of this paper is to present a deep learning approach that classifies protein diseases based on protein descriptors. Methods: Different protein descriptors are used and decomposed into modified feature descriptors. Uniquely, we introduce using Convolutional Neural Network model to learn and classify protein diseases. The modified feature descriptors are fed to the Convolutional Neural Network model on a dataset of 1563 protein sequences classified into 3 different disease classes: Aids, Tumor suppressor, and Proto oncogene. Results: The usage of the modified feature descriptors shows a significant increase in the performance of the Convolutional Neural Network model over Support Vector Machine using different kernel functions. One modified feature descriptor improved by 19.8%, 27.9%, 17.6%, 21.5%, 17.3%, and 22% for evaluation metrics: Area Under the Curve, Matthews Correlation Coefficient, Accuracy, F1-score, Recall, and Precision, respectively. Conclusion: Results show that the prediction of the proposed modified feature descriptors significantly surpasses that of Support Vector Machine model.


Sign in / Sign up

Export Citation Format

Share Document