scholarly journals Identification and Characterization of LaSCL6 Alleles in Larix kaempferi (Lamb.) Carr. Based on Analysis of Simple Sequence Repeats and Allelic Expression

Forests ◽  
2020 ◽  
Vol 11 (12) ◽  
pp. 1296
Author(s):  
Qiao-Lu Zang ◽  
Xiang-Yi Li ◽  
Li-Wang Qi ◽  
Wan-Feng Li

Simple sequence repeats (SSRs) are widely used as markers for the assessment of genetic diversity and marker-assisted breeding. In a previous study, two SSRs (GCA and CCA), were found in the genomic sequence of Larix (La) SCL6, which plays important roles in the growth and development of Larix kaempferi (Lamb.) Carr. In this study, we analyzed the polymorphisms of these two SSRs in the L. kaempferi population. We found that each SSR had five different polymorphisms, among which (GCA)7 and (CCA)7 were predominant. In addition, 12 haplotypes were detected, with (GCA)7(CCA)7 having the highest frequency. Furthermore, we detected the haplotypes of LaSCL6 in mature trees and their seeds and analyzed the relationships between parents and offspring. The expression patterns of five LaSCL6 alleles were analyzed and they showed balanced expression during vegetative development. Taken together, these findings not only provide more genetic information on LaSCL6, but also provide a candidate marker for genetic studies and breeding.

Gene ◽  
2003 ◽  
Vol 322 ◽  
pp. 85-92 ◽  
Author(s):  
Jian Yang ◽  
Jinhua Wang ◽  
Lihong Chen ◽  
Jun Yu ◽  
Jie Dong ◽  
...  

2017 ◽  
Vol 139 ◽  
pp. 113-122 ◽  
Author(s):  
Gabriela K. Orquera-Tornakian ◽  
Patricia Garrido ◽  
Brent Kronmiller ◽  
Robert Hunger ◽  
Brett M. Tyler ◽  
...  

2021 ◽  
Vol 22 (9) ◽  
pp. 4634
Author(s):  
Wenxuan Du ◽  
Junfeng Yang ◽  
Lin Ma ◽  
Qian Su ◽  
Yongzhen Pang

The calcineurin B-like protein (CBL) and CBL-interacting protein kinase (CIPK) play important roles in plant signal transduction and response to abiotic stress. Plants of Medicago genus contain many important forages, and their growth is often affected by a variety of abiotic stresses. However, studies on the CBL and CIPK family member and their function are rare in Medicago. In this study, a total of 23 CBL and 58 CIPK genes were identified from the genome of Medicago sativa as an important forage crop, and Medicaog truncatula as the model plant. Phylogenetic analysis suggested that these CBL and CIPK genes could be classified into five and seven groups, respectively. Moreover, these genes/proteins showed diverse exon-intron organizations, architectures of conserved protein motifs. Many stress-related cis-acting elements were found in their promoter region. In addition, transcriptional analyses showed that these CBL and CIPK genes exhibited distinct expression patterns in various tissues, and in response to drought, salt, and abscisic acid treatments. In particular, the expression levels of MtCIPK2 (MsCIPK3), MtCIPK17 (MsCIPK11), and MtCIPK18 (MsCIPK12) were significantly increased under PEG, NaCl, and ABA treatments. Collectively, our study suggested that CBL and CIPK genes play crucial roles in response to various abiotic stresses in Medicago.


2011 ◽  
Vol 30 (4) ◽  
pp. 827-837 ◽  
Author(s):  
Sarah M. Potts ◽  
Yuepeng Han ◽  
M. Awais Khan ◽  
Mosbah M. Kushad ◽  
A. Lane Rayburn ◽  
...  

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