scholarly journals Influence of Endogenous Factors of Food Matrices on Avidin–Biotin Immunoassays for the Detection of Bacitracin and Colistin in Food

Foods ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 219
Author(s):  
Maksim A. Burkin ◽  
Inna A. Galvidis ◽  
Sergei A. Eremin
Keyword(s):  
Clinical Practice ◽  
Food Safety ◽  
Beef Liver ◽  
Endogenous Factors ◽  
Protein Coating ◽  
Analytical Properties ◽  
Food Matrices ◽  
Matrix Free ◽  
Endogenous Biotin

(Strept)avidin–biotin technology is frequently used in immunoassay systems to improve their analytical properties. It is known from clinical practice that many (strept)avidin–biotin-based tests provide false results when analyzing patient samples with a high content of endogenous biotin. No specific investigation has been carried out regarding possible interferences from avidin (AVI) and biotin (B7) contained in food matrices in (strept)avidin–biotin-based immunoanalytical systems for food safety. Two kinds of competitive ELISAs for bacitracin (BT) and colistin (COL) determination in food matrices were developed based on conventional hapten–protein coating conjugates and biotinylated BT and COL bound to immobilized streptavidin (SAV). Coating SAV–B7–BT and SAV–B7–COL complexes-based ELISAs provided 2- and 15-times better sensitivity in BT and COL determination, corresponding to 0.6 and 0.3 ng/mL, respectively. Simultaneously with the determination of the main analytes, these kinds of tests were used as competitive assays for the assessment of AVI or B7 content up to 10 and 1 ng/mL, respectively, in food matrices (egg, infant milk formulas enriched with B7, chicken and beef liver). Matrix-free experiments with AVI/B7-enriched solutions showed distortion of the standard curves, indicating that these ingredients interfere with the adequate quantification of analytes. Summarizing the experience of the present study, it is recommended to avoid immunoassays based on avidin–biotin interactions when analyzing biosamples containing these endogenous factors or enriched with B7.

ICP- quadrupole MS for accurate determination of chromium in environmental and food matrices

2020 ◽  
pp. 100421
Author(s):  
Josiane B. Vera ◽  
Márcia C. Bisinoti ◽  
Clarice D.B. Amaral ◽  
Mario H. Gonzalez
Keyword(s):  
Accurate Determination ◽  
Food Matrices

The significance of some exogenous and endogenous factors for the forensic determination of age of wounds

1975 ◽  
Vol 5 (2) ◽  
pp. 107 ◽  
Author(s):  
S. Berg ◽  
G. Bode ◽  
G. Garbe ◽  
U. Sillius
Keyword(s):  
Endogenous Factors

Recent advancements in analytical methods for the determination of steroidal estrogen residues in environmental and food matrices

2016 ◽  
Vol 8 (28) ◽  
pp. 5556-5568 ◽  
Author(s):  
Sameera R. Gunatilake ◽  
Vihanga K. Munasinghe ◽  
Ruchiranga Ranaweera ◽  
Todd E. Mlsna ◽  
Kang Xia
Keyword(s):  
Analytical Methods ◽  
Food Samples ◽  
Steroidal Estrogen ◽  
Food Matrices

Residual steroidal estrogens in environmental and food samples have become a cause for concern as they can affect organisms at low ppt concentrations.

Innovative Industrial Processes in the production chain of maize used for human food and relevant economic results

2010 ◽  
pp. 203-222
Author(s):  
Teresina Mancuso ◽  
Stefano Massaglia ◽  
Massimo Morganti
Keyword(s):  
Food Safety ◽  
Northern Italy ◽  
Industrial Processes ◽  
Economic Sustainability ◽  
High Food ◽  
Production Chain ◽  
Human Food ◽  
Grain Processing ◽  
Grain Products

The main problem concerning the production of grain corn for human food use is on the fumonisins contamination. In the European context, the limits relating to the maximum levels allowed for these metabolites in foodstuffs, necessitate by companies involved in cultivation and in the subsequent stages of storage and processing, to bear costs and investments to get finished products conform to that regulation. The aim of this work has been assessing the economic sustainability of interventions based on particular techniques of grain processing and the use of innovative plants by elevators and processors in order to reduce the level of mycotoxin contamination. The firms examined are a storage center and a mill, located in Northern Italy, which orient their processes in order to achieve production of high food quality and health. The determination of costs and revenues to obtain grain products for human food use, has highlighted that a focused approach on the quality productions permit to obtain broader margin gains and food safety for the consumer.

Simultaneous Determination of Aflatoxin B1 and Aflatoxin M1 in Food Matrices by Enzyme-Linked Immunosorbent Assay

2012 ◽  
Vol 6 (3) ◽  
pp. 767-774 ◽  
Author(s):  
Wenxiao Jiang ◽  
Zhanhui Wang ◽  
Greta Nölke ◽  
Jing Zhang ◽  
Lanlan Niu ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Immunosorbent Assay ◽  
Aflatoxin B1 ◽  
Aflatoxin M1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Food Matrices

scholarly journals Silver nanoparticles-based plasmonic assay for the determination of sugar content in food matrices

2019 ◽  
Vol 1051 ◽  
pp. 129-137 ◽  
Author(s):  
Flavio Della Pelle ◽  
Annalisa Scroccarello ◽  
Simona Scarano ◽  
Dario Compagnone
Keyword(s):  
Silver Nanoparticles ◽  
Sugar Content ◽  
Food Matrices ◽  
Plasmonic Assay

scholarly journals Assessment of the Non-Cystic Fibrosis Bronchiectasis Severity: The FACED Score vs the Bronchiectasis Severity Index

2015 ◽  
Vol 9 (1) ◽  
pp. 46-51 ◽  
Author(s):  
J Minov ◽  
J Karadzinska-Bislimovska ◽  
K Vasilevska ◽  
S Stoleski ◽  
D Mijakoski
Keyword(s):  
Body Mass Index ◽  
Cystic Fibrosis ◽  
Clinical Practice ◽  
Observational Study ◽  
Body Mass ◽  
Severity Index ◽  
Sufficient Power ◽  
Chronic Colonization ◽  
The Mean

Introduction : Non-cystic fibrosis bronchiectasis (NCFB) is a multidimensional disease, and no single isolated parameter is proved to have sufficient power for any overall determination of its severity and prognosis. Objective : To compare the results of the assessment of the NCFB severity with respect to its prognosis in the same patients by two different validated scores, i.e. the FACED score and the Bronchiectasis Severity Index (BSI). Methods : An observational study including 37 patients with NCFB (16 males and 21 female aged 46 to 76 years) was performed. All patients underwent evaluation of the variables incorporated in the FACED score (FEV1 % predicted, age, chronic colonization by Pseudomaonas aeruginosa, radiological extent of the disease, and dyspnea) and in the BSI (age, body mass index, FEV1 % predicted, hospitalization and exacerbations in previous year, dyspnea, chronic colonization by Pseudomaonas aeruginosa and other microrganisms, and radiological extent of the disease). Results : According to the value of the derived overall FACED score we found 17 patients (45.9%) with mild bronchiectasis, 14 patients (37.8%) with moderate bronchiectasis and 6 patients (16.2%) with severe bronchiectasis. The mean derived FACED score was 3.4 ± 1.3. In addition, according to the value of the derived overall BSI score, the frequency of patients with low, intermediate and high BSI score was 16 patients (43,2%), 14 patients (37.8%) and 7 patients (18.9%), respectively. The mean derived BSI score was 6.4 ± 2.5. Conclusion : We found similar results by the assessment of the NCFB severity in regard to its prognosis by both the FACED score and the BSI. Further studies determining how these scores may impact clinical practice are needed.

Sign in / Sign up

Export Citation Format

Share Document