scholarly journals Structural Characterization of Quinoa Polysaccharide and Its Inhibitory Effects on 3T3-L1 Adipocyte Differentiation

Foods ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1511
Author(s):  
Cong Teng ◽  
Zhenxing Shi ◽  
Yang Yao ◽  
Guixing Ren
Keyword(s):  
Adipocyte Differentiation ◽  
Monosaccharide Composition ◽  
Structural Formula ◽  
Gas Chromatography Mass Spectrometry ◽  
Glycosidic Linkage ◽  
Food Crop ◽  
Inhibitory Effects ◽  
Rt Pcr ◽  
Pcr Assays

Quinoa is a kind of nutritious food crop with anti-obesity activity, however, the mechanism is not unclear. In this study, we separated and purified bioactive polysaccharide from quinoa (denoted SQWP-2). The chemical structural was characterized and its effect on 3T3-L1 pre-adipocyte differentiation was evaluated. The molecular weight of SQWP-2 was found to be 7.49 × 103 Da, and the polysaccharide consisted of fructose and glucose. The Glc-(1→, Fru-(2→, →4)-Glcp-(1→, and →4,6)-Glcp-(1→ glycosidic linkages were identified in SQWP-2 through gas chromatography-mass spectrometry. Nuclear magnetic resonance confirmed the monosaccharide composition and glycosidic linkage content, and a suggestion of the structural formula is provided. In Western Blotting and RT-PCR assays, treatment with SQWP-2 significantly inhibited 3T3-L1 differentiation by suppressing PPARγ, C/EBPα, C/EBPβ, C/EBPδ, SREBP1C and AP2 expression. Quinoa polysaccharide isolated here could represent an anti-obesity agent once the structures and differentiation inhibition are definitively characterized.

scholarly journals A Synergistic Anti-Diabetic Effect by Ginsenosides Rb1 and Rg3 through Adipogenic and Insulin Signaling Pathways in 3T3-L1 Cells

2021 ◽  
Vol 11 (4) ◽  
pp. 1725
Author(s):  
Hee-Do Hong ◽  
Sun-Il Choi ◽  
Ok-Hwan Lee ◽  
Young-Cheul Kim
Keyword(s):  
Transcription Factors ◽  
Insulin Signaling ◽  
Adipocyte Differentiation ◽  
Red Ginseng ◽  
Inhibitory Effects ◽  
Rt Pcr ◽  
High Concentration ◽  
Expression Of Genes ◽  
The Individual ◽  
Maximal Expression

Although ginsenosides Rb1 and Rg3 have been identified as the significant ginsenosides found in red ginseng that confer anti-diabetic actions, it is unclear whether insulin-sensitizing effects are mediated by the individual compounds or by their combination. To determine the effect of ginsenosides Rb1 and Rg3 on adipocyte differentiation, 3T3-L1 preadipocytes were induced to differentiate the standard hormonal inducers in the absence or presence of ginsenosides Rb1 or Rg3. Additionally, we determined the effects of Rb1, Rg3, or their combination on the expression of genes related to adipocyte differentiation, adipogenic transcription factors, and the insulin signaling pathway in 3T3-L1 cells using semi-quantitative RT-PCR. Rb1 significantly increased the expression of CEBPα, PPARγ, and aP2 mRNAs. However, Rg3 exerted its maximal stimulatory effect on these genes at 1 μM concentration, while a high concentration (50 μM) showed inhibitory effects. Similarly, treatment with Rb1 and Rg3 (1 μM) increased the expression of IRS-1, Akt, PI3K, GLUT4, and adiponectin. Importantly, co-treatment of Rb1 and Rg3 (9:1) induced the maximal expression levels of these mRNAs. Our data indicate that the anti-diabetic activity of red ginseng is, in part, mediated by synergistic actions of Rb1 and Rg3, further supporting the significance of minor Rg3.

scholarly journals The Recombinant Potato virus Y (PVY) Strain, PVYNTN, Identified in Potato Fields in Victoria, Southeastern Australia

Plant Disease ◽  
2020 ◽  
Vol 104 (12) ◽  
pp. 3110-3114
Author(s):  
Mariana Rodriguez-Rodriguez ◽  
Mohamad Chikh-Ali ◽  
Steven B. Johnson ◽  
Stewart M. Gray ◽  
Nellie Malseed ◽  
...  
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Strain Typing ◽  
Rt Pcr ◽  
Growing Seasons ◽  
Fta Cards ◽  
Southeastern Australia ◽  
Whole Genomes ◽  
Pcr Assays

Potato virus Y (PVY) is one of the main viruses affecting potato in Australia. However, molecular characterization of PVY isolates circulating in potato in different states of Australia has not yet been thoroughly conducted. Only nonrecombinant isolates of three biological PVY strains collected from potato were reported previously from Western Australia and one from Queensland. Here, PVY isolates collected from seed potato originating in Victoria, Australia, and printed on FTA cards, were subjected to strain typing by RT-PCR, with three isolates subjected to whole genome sequencing. All the 59 PVY isolates detected during two growing seasons were identified to be recombinants based on two RT-PCR assays. No nonrecombinant PVY isolates were identified. All the RT-PCR typed isolates belonged to the PVYNTN strain. Sequence analysis of the whole genomes of three isolates suggested a single introduction of the PVYNTN strain to Australia but provided no clues as to where this introduction originated. Given the association of the PVYNTN strain with potato tuber damage, growers in Australia should implement appropriate strategies to manage PVYNTN in potato.

Validation of RT-PCR Assays for Molecular Characterization of Porcine Teschoviruses and Enteroviruses

2006 ◽  
Vol 53 (6) ◽  
pp. 257-265 ◽  
Author(s):  
G. La Rosa ◽  
M. Muscillo ◽  
A. Di Grazia ◽  
S. Fontana ◽  
M. Iaconelli ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Rt Pcr ◽  
Pcr Assays

Development and validation of cytokine quantitative, real time RT-PCR assays for characterization of Asian elephant immune responses

2009 ◽  
Vol 131 (1-2) ◽  
pp. 73-78 ◽  
Author(s):  
Jennifer A. Landolfi ◽  
Stacy A. Schultz ◽  
Susan K. Mikota ◽  
Karen A. Terio
Keyword(s):  
Immune Responses ◽  
Real Time ◽  
Asian Elephant ◽  
Rt Pcr ◽  
Pcr Assays ◽  
Development And Validation

Characterization of specific cDNA background synthesis introduced by reverse transcription in RT-PCR assays

Biochimie ◽  
2010 ◽  
Vol 92 (12) ◽  
pp. 1839-1846 ◽  
Author(s):  
M.F. Adrover ◽  
M.J. Muñoz ◽  
M.V. Baez ◽  
J. Thomas ◽  
A.R. Kornblihtt ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Rt Pcr ◽  
Pcr Assays

scholarly journals Characterization of Human Coronavirus Etiology in Chinese Adults with Acute Upper Respiratory Tract Infection by Real-Time RT-PCR Assays

PLoS ONE ◽  
2012 ◽  
Vol 7 (6) ◽  
pp. e38638 ◽  
Author(s):  
Roujian Lu ◽  
Xiaoyan Yu ◽  
Wen Wang ◽  
Xijie Duan ◽  
Linglin Zhang ◽  
...  
Keyword(s):  
Tract Infection ◽  
Respiratory Tract ◽  
Respiratory Tract Infection ◽  
Upper Respiratory Tract ◽  
Rt Pcr ◽  
Chinese Adults ◽  
Human Coronavirus ◽  
Upper Respiratory ◽  
Pcr Assays
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