scholarly journals Molecular Cloning, Structure and Phylogenetic Analysis of a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca)

Genes ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 93
Author(s):  
Elena Todorovska ◽  
Martin Ivanov ◽  
Mariana Radkova ◽  
Alexandar Dolashki ◽  
Pavlina Dolashka
Keyword(s):  
Phylogenetic Analysis ◽  
Black Sea ◽  
The Black Sea ◽  
Copper Binding ◽  
Amino Acid Residues ◽  
Panulirus Interruptus ◽  
Reading Frame ◽  
Est Analysis ◽  
Physiological Processes ◽  
Rapid Amplification

Hemocyanins are copper-binding proteins that play a crucial role in the physiological processes in crustaceans. In this study, the cDNA encoding hemocyanin subunit 5 from the Black sea crab Eriphia verrucosa (EvHc5) was cloned using EST analysis, RT-PCR and rapid amplification of the cDNA ends (RACE) approach. The full-length cDNA of EvHc5 was 2254 bp, consisting of a 5′ and 3′ untranslated regions and an open reading frame of 2022 bp, encoding a protein consisting of 674 amino acid residues. The protein has an N-terminal signal peptide of 14 amino acids as is expected for proteins synthesized in hepatopancreas tubule cells and secreted into the hemolymph. The 3D model showed the presence of three functional domains and six conserved histidine residues that participate in the formation of the copper active site in Domain 2. The EvHc5 is O-glycosylated and the glycan is exposed on the surface of the subunit similar to Panulirus interruptus. The phylogenetic analysis has shown its close grouping with γ-type of hemocyanins of other crustacean species belonging to order Decapoda, infraorder Brachyura.

Molecular cloning and characterization of a LEAFY-like gene highly expressed in developing soybean seeds

2007 ◽  
Vol 17 (4) ◽  
pp. 297-302 ◽  
Author(s):  
Qingchang Meng ◽  
Chunhong Zhang ◽  
Fang Huang ◽  
Junyi Gai ◽  
Deyue Yu
Keyword(s):  
Transcription Factors ◽  
Seed Development ◽  
Genomic Structure ◽  
Soybean Seed ◽  
Reproductive Organs ◽  
Pcr Analysis ◽  
Amino Acid Residues ◽  
Reading Frame ◽  
Rapid Amplification ◽  
Onion Epidermal Cells

AbstractLEAFY (LFY)-like proteins are plant-specific transcription factors that play essential roles in plant growth and development. In this study, a LEAFY homologue in soybean [Glycine max (L.) Merr.], designated as GmLFY, was cloned from inflorescences by the rapid amplification of cDNA ends (RACE) method. Sequence analysis showed that GmLFY cDNA contained a 1221 bp open reading frame, encoding 407 amino acid residues with typical characteristics of transcription factors. Subcellular localization in onion epidermal cells indicated that GmLFY protein was located in the nucleus. Analysis of the GmLFY genomic structure showed that the GmLFY gene has two introns with similar spliced sites as LFY-like genes in other plants. Reverse transcription-polymerase chain reaction (RT-PCR) analysis indicated that GmLFY was predominantly expressed in reproductive organs, such as inflorescences, pods and developing seeds. Further analysis showed that the expression level of GmLFY was higher in the middle stage than in the early or late stages of seed development, suggesting an essential regulatory role of GmLFY in soybean seed development.

Molecular cloning and assessment of the immunocontraceptive potential of the zona pellucida subunit 3 from Brandt's vole (Microtus brandti)

2006 ◽  
Vol 18 (3) ◽  
pp. 331 ◽  
Author(s):  
Hui Li ◽  
Yun-shang Piao ◽  
Zhi-bin Zhang ◽  
Christopher M. Hardy ◽  
Lyn A. Hinds
Keyword(s):  
Amino Acid ◽  
Zona Pellucida ◽  
Keyhole Limpet Hemocyanin ◽  
Amino Acid Residues ◽  
Igg Antibody ◽  
Reading Frame ◽  
Rapid Amplification ◽  
Ovarian Pathology ◽  
Race Pcr ◽  
Brandt’S Vole

A full-length cDNA encoding Brandt’s vole (Microtus brandti) zona pellucida glycoprotein subunit 3 (vZP3) was isolated using rapid amplification of cDNA ends–polymerase chain reaction (RACE-PCR). The cDNA contains an open reading frame of 1254 nucleotides encoding a polypeptide of 418 amino acid residues. The deduced amino acid sequence of vZP3 revealed high overall homology with hamster (82.1%), mouse (81.3%) and rat (80.6%). A synthetic vZP3 peptide corresponding to amino acid residues 328–343 was conjugated to keyhole limpet hemocyanin (KLH-vZP3328–343) and used to immunise female Brandt’s voles in order to test the efficacy of this peptide as a contraceptive antigen. High IgG antibody levels to the vZP3328–343 peptide were present in the sera of female voles immunised with KLH-vZP3328–343 and these also cross-reacted to the zona pellucida in ovaries of Brandt’s vole. The fertility of the KLH-vZP3328–343-immunised voles was reduced by 50% compared with controls without evidence of significant ovarian pathology.

Molecular Cloning and Characterization of a Novel Dehydrin Gene from Ginkgo biloba

2006 ◽  
Vol 26 (3) ◽  
pp. 203-215 ◽  
Author(s):  
Zhongxiang Deng ◽  
Yiding Wang ◽  
Keji Jiang ◽  
Xuefen Liu ◽  
Weisheng Wu ◽  
...  
Keyword(s):  
Ginkgo Biloba ◽  
Genomic Sequence ◽  
Gene Promoter ◽  
Single Copy ◽  
Pcr Analysis ◽  
Amino Acid Residues ◽  
Living Fossil ◽  
Reading Frame ◽  
Shared Identity ◽  
Rapid Amplification

A full-length cDNA encoding a dehydrin was cloned from the living fossil plant Ginkgo biloba by rapid amplification of cDNA ends (RACE). The cDNA, designated as GbDHN, was 813 bp long containing an open reading frame of 489 bp. The deduced GbDHN protein had 163 amino acid residues, which formed a 17 kDa polypeptide with a predicted isoelectric point (pI) of 5.75. GbDHN had an S-segment and a K-segment, indicative of dehydrins, but no Y-segments. Homology analysis indicated that the S-segment and K-segment of GbDHN shared identity with those of other reported dehydrins, indicating that GbDHN belonged to dehydrin superfamily. Genomic sequence of GbDHN was also cloned using genomic walker technology. By comparing genomic DNA with the cDNA, it was found that there was a 257-bp intron in this gene. Promoter analysis indicated that it contained six CAAT boxes, one TATA box, one ABRE box and one GC-motif in the 5′-flanking region. Southern blot analysis revealed that GbDHN belonged to a single copy gene family. RT-PCR analysis revealed that GbDHN constitutively expressed in stems and roots. The increased expression of GbDHN was detected when G. biloba seedlings were treated with exogenous abscisic acid (ABA), salt stress and drought stress. These results indicate that the GbDHN has the potential to play a role in response to ABA and environmental stresses that can cause plant dehydration.

MÖSSBAUER AND CHEMICAL ANALYSES OF SOME SEDIMENTS FROM THE ROMANIAN SHORE OF THE BLACK SEA

1979 ◽  
Vol 40 (C2) ◽  
pp. C2-445-C2-448
Author(s):  
D. Barb ◽  
L. Diamandescu ◽  
M. Morariu ◽  
I. I. Georgescu
Keyword(s):  
Black Sea ◽  
The Black Sea ◽  
Chemical Analyses

Modeling of ecological conditions in the Euxine Basin in the Sarmatian and Maeotian by methods of diatom analysis

2019 ◽  
pp. 257-261
Author(s):  
Eleonora P. Radionova
Keyword(s):  
Black Sea ◽  
Late Miocene ◽  
The Black Sea ◽  
Subtidal Zone ◽  
Diatom Analysis ◽  
Diatom Flora ◽  
North West ◽  
Ecological Conditions ◽  
The North

The associations and ecological conditions of the existence of modern diatoms of the North-West (Pridneprovsky), Prikerchensky and Eastern regions of the subtidal zone of the Black Sea are considered. Based on the unity of the composition of the Present and Sarmatian-Meotian diatom flora, an attempt has been made to model some of the ecological c situation of the Late Miocene Euxinian basin.

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