scholarly journals Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques

2021 ◽  
Vol 13 (2) ◽  
pp. 518-539
Author(s):  
Peuli Nath ◽  
Md Alamgir Kabir ◽  
Somaiyeh Khoubafarin Doust ◽  
Aniruddha Ray

Herpes is a widespread viral infection caused by the herpes simplex virus (HSV) that has no permanent cure to date. There are two subtypes, HSV-1 and HSV-2, that are known to cause a variety of symptoms, ranging from acute to chronic. HSV is highly contagious and can be transmitted via any type of physical contact. Additionally, viral shedding can also happen from asymptomatic infections. Thus, early and accurate detection of HSV is needed to prevent the transmission of this infection. Herpes can be diagnosed in two ways, by either detecting the presence of the virus in lesions or the antibodies in the blood. Different detection techniques are available based on both laboratory and point of care (POC) devices. Laboratory techniques include different biochemical assays, microscopy, and nucleic acid amplification. In contrast, POC techniques include microfluidics-based tests that enable on-spot testing. Here, we aim to review the different diagnostic techniques, both laboratory-based and POC, their limits of detection, sensitivity, and specificity, as well as their advantages and disadvantages.

2009 ◽  
Vol 20 (4) ◽  
pp. 122-125 ◽  
Author(s):  
Guy Boivin ◽  
Brigitte Malette ◽  
Nathalie Goyette

BACKGROUND: Primary herpes simplex virus (HSV) infection usually involves one mucosal region.OBJECTIVE: To describe an unusual disseminated HSV-1 infection involving two mucosal sites in a healthy man.RESULTS: Primary HSV infection involved oral and genital mucosa and was associated with viremia, hepatitis and rash. Phylogenetic analysis of genital and oral viruses revealed that the patient was infected by a single HSV-1 strain.CONCLUSION: Use of polymerase chain reaction detection techniques for HSV may identify viremic patients in the absence of obvious immunosuppression.


2009 ◽  
Vol 44 (2) ◽  
pp. 125-128 ◽  
Author(s):  
Bridget Leyland ◽  
Margaret R. Kennedy ◽  
Yolanda H. Wimberly ◽  
Bruce J. Levine ◽  
Thomas L. Cherpes

2005 ◽  
Vol 16 (2) ◽  
pp. 92-98 ◽  
Author(s):  
Ameeta Singh ◽  
Jutta Preiksaitis ◽  
Barbara Romanowski

Herpes simplex virus (HSV) types 1 and 2 cause genital herpes infections and are the most common cause of genital ulcer disease in industrialized nations. Although these infections are very common, the majority of them remain underdiagnosed because they are asymptomatic or unrecognized. A clinical diagnosis of genital herpes should always be confirmed by laboratory testing; this can be accomplished through the use of direct tests for viral isolation, the detection of antigen or, more recently, the detection of HSV DNA using molecular diagnostic techniques. Testing for serotypes is recommended because of the different prognostic and counselling implications. Type-specific HSV serology is becoming more readily available and will enhance the ability to make the diagnosis and guide clinical management in select patients.


2003 ◽  
Vol 28 (2) ◽  
pp. 175-185 ◽  
Author(s):  
Lottie Schloss ◽  
Anton M van Loon ◽  
Paola Cinque ◽  
Graham Cleator ◽  
José-Manuel Echevarria ◽  
...  

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Carlos A. Gomez ◽  
Benjamin A. Pinsky ◽  
Anne Liu ◽  
Niaz Banaei

Abstract The FilmArray meningitis/encephalitis (ME) panel is a novel syndromic, nucleic acid amplification test for diagnosis of acute meningitis and encephalitis. Emerging data on its performance are concerning for false-positive results. We present a case of tuberculous meningitis misdiagnosed as herpes simplex virus-1 encephalitis with the FilmArray ME panel. Strategies to mitigate erroneous results are discussed.


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