Urinary Proteomics of Simulated Firefighting Tasks and Its Relation to Fitness Parameters

Firefighting rescues are high-hazard activities accompanied by uncertainty, urgency, and complexity. Knowledge of the metabolic characteristics during firefighting rescues is of great value. The purpose of this study was to explore the firefighting-induced physiological responses in greater depth. The urine samples of ten firefighters were collected before and after the simulated firefighting, and the proteins in urine samples were identified by the liquid chromatography–mass spectroscopy. Blood lactate and heart rate were measured. There were 360 proteins up-regulated and 265 proteins downregulated after this simulated firefighting. Changes in protein expression were significantly related to acute inflammatory responses, immune responses, complement activation, and oxidative stress. Beta-2-microglobulin (r = 0.76, p < 0.05) and von Willebrand factors (r = 0.81, p < 0.01) were positively correlated with heart rate during simulated firefighting, and carbonic anhydrase 1 (r = 0.67, p < 0.05) were positively correlated with blood lactate after simulated firefighting. These results illustrated that Beta-2-microglobulin, von Willebrand, and carbonic anhydrase 1 could be regarded as important indicators to evaluate exercise intensity for firefighters.

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Einfluss einer intensiven Intervallbelastung auf die Beanspruchung der kortikalen Gehirnaktivität

Swiss Sports & Exercise Medicine ◽

10.34045/ssem/2015/3 ◽

2015 ◽

Vol 63 (1) ◽

Keyword(s):

Heart Rate ◽

Blood Lactate ◽

Nervous Activity ◽

Lactate Concentration ◽

Blood Lactate Concentration ◽

Interval Training ◽

Cortical Activity ◽

Active Recovery ◽

Warm Up ◽

Beta 2

Due to methodological and technical challenges brain cortical activity has rarely been investigated during endurance exercise. In this respect, it is not surprising that effects of an acute bout of interval training on central nervous activity have not been examined yet. Therefore, the aim of the present investigation was to characterize acute adaptations of brain cortical activity and established parameters to a high intensity endurance session. In a laboratory study sixteen endurance-trained cyclists completed an exercise bout including 3 interval series on a high-performance bicycle ergometer. Changes in cortical activity were recorded with quantitative electroencephalography (EEG) and analyzed in five specific frequency ranges (theta, alpha-1, alpha-2, beta-1, beta-2). Additionally, heart rate, blood lactate concentration and received perception of effort (RPE) were measured. During warm-up brain cortical activity increased above resting levels. Compared to warm-up and active recovery, EEG spectral power in Alpha-2- and Beta-2-band was higher in each interval series. Similarly, heart rate, blood lactate concentration and RPE increased from active recovery to the following interval loads. Whereas those parameters also increased from the first to the last series of intervals, a significant reduction of spectral EEG power was recorded in the theta-, alpha-2-, beta-1- and beta-2-band. The results provide evidence on specific regulations of brain cortical activity during interval training. Gained insights on the dose-response relationship can be transferred into the training practice to optimize load control.

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Beta-2 Microglobulin (B2 M) Genomic Alterations and Absent Protein Expression in Pediatric and Adolescent Classical Hodgkin Lymphoma

Klinische Pädiatrie ◽

10.1055/s-0034-1371104 ◽

2014 ◽

Vol 226 (02) ◽

Cited By ~ 1

Author(s):

L Giulino-Roth ◽

J Reichel ◽

J Teruya-Feldstein ◽

W Tam ◽

Y Tam ◽

...

Keyword(s):

Protein Expression ◽

Hodgkin Lymphoma ◽

Classical Hodgkin Lymphoma ◽

Genomic Alterations ◽

Beta 2 ◽

Beta 2 Microglobulin

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Wearing surgical masks does not affect heart rate and blood lactate accumulation during cycle ergometer exercise

The Journal of Sports Medicine and Physical Fitness ◽

10.23736/s0022-4707.20.11378-1 ◽

2020 ◽

Vol 60 (11) ◽

Author(s):

Lorenzo Boldrini ◽

Furio Danelon ◽

Davide Fusetti ◽

Giacomo Lucenteforte ◽

Giulio S. Roi

Keyword(s):

Heart Rate ◽

Blood Lactate ◽

Cycle Ergometer ◽

Lactate Accumulation ◽

Cycle Ergometer Exercise ◽

Surgical Masks ◽

Ergometer Exercise ◽

Blood Lactate Accumulation ◽

Affect Heart Rate

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Faculty Opinions recommendation of Activation of nonclassical CD1d-restricted NK T cells induces airway hyperreactivity in beta 2-microglobulin-deficient mice.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1122835.579924 ◽

2008 ◽

Author(s):

Santa Jeremy Ono

Keyword(s):

T Cells ◽

Airway Hyperreactivity ◽

Nk T Cells ◽

Beta 2 ◽

Beta 2 Microglobulin ◽

Deficient Mice

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Quantitative determination of the beta-2-microglobulin monomer by gel filtration chromatography

Chinese Journal of Chromatography ◽

10.3724/sp.j.1123.2018.11028 ◽

2019 ◽

Vol 37 (5) ◽

pp. 533

Author(s):

Xiaobo BAO ◽

Jun REN ◽

Yufeng WANG ◽

Lingyun JIA

Keyword(s):

Quantitative Determination ◽

Gel Filtration ◽

Gel Filtration Chromatography ◽

Beta 2 ◽

Beta 2 Microglobulin

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Serial Quantification of Urinary Protein Biomarkers to Predict Drug-induced Acute Kidney Injury

Current Drug Metabolism ◽

10.2174/1389200220666190711114504 ◽

2019 ◽

Vol 20 (8) ◽

pp. 656-664 ◽

Cited By ~ 4

Author(s):

Yi Da ◽

K. Akalya ◽

Tanusya Murali ◽

Anantharaman Vathsala ◽

Chuen-Seng Tan ◽

...

Keyword(s):

Acute Kidney Injury ◽

Cystatin C ◽

Calcineurin Inhibitors ◽

Kidney Injury ◽

Tubular Dysfunction ◽

Renal Tubular Dysfunction ◽

Protein Biomarkers ◽

Drug Induced ◽

Beta 2 ◽

Beta 2 Microglobulin

Background: : Drug-induced Acute Kidney Injury (AKI) develops in 10-15% of patients who receive nephrotoxic medications. Urinary biomarkers of renal tubular dysfunction may detect nephrotoxicity early and predict AKI. Methods:: We prospectively studied patients who received aminoglycosides, vancomycin, amphotericin, or calcineurin inhibitors, and collected their serial urine while on therapy. Patients who developed drug-induced AKI (fulfilling KDIGO criteria) were matched with non-AKI controls in a 1:2 ratio. Their urine samples were batch-analyzed at time-intervals leading up to AKI onset; the latter benchmarked against the final day of nephrotoxic therapy in non- AKI controls. Biomarkers examined include clusterin, beta-2-microglobulin, KIM1, MCP1, cystatin-C, trefoil-factor- 3, NGAL, interleukin-18, GST-Pi, calbindin, and osteopontin; biomarkers were normalized with corresponding urine creatinine. Results:: Nine of 84 (11%) patients developed drug-induced AKI. Biomarkers from 7 AKI cases with pre-AKI samples were compared with those from 14 non-AKI controls. Corresponding mean ages were 55(±17) and 52(±16) years; baseline eGFR were 99(±21) and 101(±24) mL/min/1.73m2 (all p=NS). Most biomarker levels peaked before the onset of AKI. Median levels of 5 biomarkers were significantly higher in AKI cases than controls at 1-3 days before AKI onset (all µg/mmol): clusterin [58(8-411) versus 7(3-17)], beta-2-microglobulin [1632(913-3823) versus 253(61-791)], KIM1 [0.16(0.13-0.76) versus 0.07(0.05-0.15)], MCP1 [0.40(0.16-1.90) versus 0.07(0.04-0.17)], and cystatin-C [33(27-2990) versus 11(7-19)], all p<0.05; their AUROC for AKI prediction were >0.80 (confidence intervals >0.50), with average accuracy highest for clusterin (86%), followed by beta-2-microglobulin, cystatin-C, MCP1, and KIM1 (57%) after cross-validation. Conclusion: : Serial surveillance of these biomarkers could improve the lead time for nephrotoxicity detection by days.

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