Ectopic Expression of BraYAB1-702, a Member of YABBY Gene Family in Chinese Cabbage, Causes Leaf Curling, Inhibition of Development of Shoot Apical Meristem and Flowering Stage Delaying in Arabidopsis thaliana

Lateral organs produced by shoot apical and flower meristems exhibit a fundamental abaxial-adaxial asymmetry. We describe three members of the YABBY gene family, FILAMENTOUS FLOWER, YABBY2 and YABBY3, isolated on the basis of homology to CRABS CLAW. Each of these genes is expressed in a polar manner in all lateral organ primordia produced from the apical and flower meristems. The expression of these genes is precisely correlated with abaxial cell fate in mutants in which abaxial cell fates are found ectopically, reduced or eliminated. Ectopic expression of either FILAMENTOUS FLOWER or YABBY3 is sufficient to specify the development of ectopic abaxial tissues in lateral organs. Conversely, loss of polar expression of these two genes results in a loss of polar differentiation of tissues in lateral organs. Taken together, these observations indicate that members of this gene family are responsible for the specification of abaxial cell fate in lateral organs of Arabidopsis. Furthermore, ectopic expression studies suggest that ubiquitous abaxial cell fate and maintenance of a functional apical meristem are incompatible.

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Ectopic Expression of a Pak-choi YABBY Gene, BcYAB3, Causes Leaf Curvature and Flowering Stage Delay in Arabidopsis thaliana

Genes ◽

10.3390/genes11040370 ◽

2020 ◽

Vol 11 (4) ◽

pp. 370 ◽

Cited By ~ 1

Author(s):

Hualan Hou ◽

Ye Lin ◽

Xilin Hou

Keyword(s):

Arabidopsis Thaliana ◽

Regulatory Mechanism ◽

Critical Role ◽

Ectopic Expression ◽

Flowering Stage ◽

Specific Expression ◽

Pak Choi ◽

Lateral Organs ◽

Yabby Gene ◽

Stem Leaf

The YABBY family are a group of seed plant-specific transcription factors, which are involved in the specification of abaxial polarity in lateral organs. In Arabidopsis thaliana, YABBY3 (YAB3) plays a critical role in regulating abaxial patterning, growth of lateral organs, and inflorescence phyllotaxy. In this study, the BcYAB3 gene was isolated from Pak-choi (Brassica rapa subsp. chinensis). The tissue-specific expression analysis indicated that the BcYAB3 gene has significantly high transcript levels in stem, leaf, and flower. We investigated the subcellular localization of BcYAB3 and found the protein to be expressed in the nucleus. In the transgenic Arabidopsis thaliana plants expressing the BcYAB3 gene, the leaves were curling downward with the plant growth, and the bolting and flowering stages were delayed. These results not only validate the function of BcYAB3 in the leaf and flower development in Arabidopsis, but also contribute to unravel the molecular regulatory mechanism of YAB3 gene in the establishment of adaxial–abaxial polarity of the lateral organs in Pak-choi.

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Nuclear import of the transcription factor SHOOT MERISTEMLESS depends on heterodimerization with BLH proteins expressed in discrete sub-domains of the shoot apical meristem of Arabidopsis thaliana

Nucleic Acids Research ◽

10.1093/nar/gkl016 ◽

2006 ◽

Vol 34 (4) ◽

pp. 1281-1292 ◽

Cited By ~ 70

Author(s):

M. Cole

Keyword(s):

Arabidopsis Thaliana ◽

Transcription Factor ◽

Nuclear Import ◽

Shoot Apical Meristem ◽

Apical Meristem ◽

Shoot Meristemless

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Characterisation of three shoot apical meristem mutants of Arabidopsis thaliana

Development ◽

10.1242/dev.116.2.397 ◽

1992 ◽

Vol 116 (2) ◽

pp. 397-403 ◽

Cited By ~ 19

Author(s):

H. M. Ottoline Leyser ◽

I. J. Furner

Keyword(s):

Arabidopsis Thaliana ◽

Root Growth ◽

Shoot Apical Meristem ◽

Apical Meristem ◽

Floral Development ◽

Wild Type ◽

Recessive Mutations ◽

Phenotypic Characterisation ◽

Dicotyledonous Plants ◽

And Function

The shoot apical meristem of dicotyledonous plants is highly regulated both structurally and functionally, but little is known about the mechanisms involved in this regulation. Here we describe the genetic and phenotypic characterisation of recessive mutations at three loci of Arabidopsis thaliana in which meristem structure and function are disrupted. The loci are Clavata1 (Clv1), Fasciata1 (Fas1) and Fasciata2 (Fas2). Plants mutant at these loci are fasciated having broad, flat stems and disrupted phyllotaxy. In all cases, the fasciations are associated with shoot apical meristem enlargement and altered floral development. While all the mutants share some phenotypic features they can be divided into two classes. The pleiotropic fas1 and fas2 mutants are unable to initiate wild- type organs, show major alterations in meristem structure and have reduced root growth. In contrast, clv1 mutant plants show near wild-type organ phenotypes, more subtle changes in shoot apical meristem structure and wild-type root growth.

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