Ectopic expression of TaYAB1, a member of YABBY gene family in wheat, causes the partial abaxialization of the adaxial epidermises of leaves and arrests the development of shoot apical meristem in Arabidopsis

Plant Science ◽  
2006 ◽  
Vol 170 (2) ◽  
pp. 364-371 ◽  
Author(s):  
Wei Zhao ◽  
Hong Yan Su ◽  
Jian Song ◽  
Xiang Yu Zhao ◽  
Xian Sheng Zhang
Keyword(s):  
Gene Family ◽  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Ectopic Expression ◽  
Yabby Gene

Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

Development ◽  
1999 ◽  
Vol 126 (18) ◽  
pp. 4117-4128 ◽  
Author(s):  
K.R. Siegfried ◽  
Y. Eshed ◽  
S.F. Baum ◽  
D. Otsuga ◽  
G.N. Drews ◽  
...  
Keyword(s):  
Gene Family ◽  
Cell Fate ◽  
Apical Meristem ◽  
Ectopic Expression ◽  
Cell Fates ◽  
Lateral Organs ◽  
Expression Studies ◽  
Lateral Organ ◽  
Yabby Gene ◽  
Filamentous Flower

Lateral organs produced by shoot apical and flower meristems exhibit a fundamental abaxial-adaxial asymmetry. We describe three members of the YABBY gene family, FILAMENTOUS FLOWER, YABBY2 and YABBY3, isolated on the basis of homology to CRABS CLAW. Each of these genes is expressed in a polar manner in all lateral organ primordia produced from the apical and flower meristems. The expression of these genes is precisely correlated with abaxial cell fate in mutants in which abaxial cell fates are found ectopically, reduced or eliminated. Ectopic expression of either FILAMENTOUS FLOWER or YABBY3 is sufficient to specify the development of ectopic abaxial tissues in lateral organs. Conversely, loss of polar expression of these two genes results in a loss of polar differentiation of tissues in lateral organs. Taken together, these observations indicate that members of this gene family are responsible for the specification of abaxial cell fate in lateral organs of Arabidopsis. Furthermore, ectopic expression studies suggest that ubiquitous abaxial cell fate and maintenance of a functional apical meristem are incompatible.

scholarly journals Sample preparation for laser-microdissection of soybean shoot apical meristem

2012 ◽  
Vol 3 (1) ◽  
pp. 3 ◽  
Author(s):  
Chui E. Wong ◽  
Mohan B. Singh ◽  
Prem L. Bhalla
Keyword(s):  
Sample Preparation ◽  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Laser Microdissection ◽  
Specific Cell ◽  
Legume Crop ◽  
Continuous Formation ◽  
Molecular Events ◽  
Depth Analysis ◽  
Specialized Equipment

The shoot apical meristem houses stem cells responsible for the continuous formation of aerial plant organs including leaves and stems throughout the life of plants. Laser-microdissection in combination with high-throughput technology such as next generation sequencing permits an in-depth analysis of molecular events associated with specific cell type of interest. Sample preparation is the most critical step in ensuring good quality RNA to be extracted from samples following laser-microdissection. Here, we optimized the sample preparation for a major legume crop, soybean. We used Farmer’s solution as a fixative and paraffin as the embedding medium for soybean shoot apical meristem tissue without the use of any specialized equipment. Shorter time for tissue fixation (two days) was found to be critical for the preservation of RNA in soybean shoot apical meristem. We further demonstrated the utility of this method for different tissues derived from soybean and rice. The method outlined here shall facilitate studies on crop plants involving laser-microdissection.

Somatic embryogenesis from Arabidopsis shoot apical meristem mutants

Planta ◽  
2002 ◽  
Vol 214 (6) ◽  
pp. 829-836 ◽  
Author(s):  
Andreas Mordhorst ◽  
Marijke Hartog ◽  
Mazen El Tamer ◽  
Thomas Laux ◽  
Sacco de Vries
Keyword(s):  
Somatic Embryogenesis ◽  
Shoot Apical Meristem ◽  
Apical Meristem

scholarly journals Arabidopsis Argonaute10 Specifically Sequesters miR166/165 to Regulate Shoot Apical Meristem Development

Cell ◽  
2011 ◽  
Vol 145 (2) ◽  
pp. 242-256 ◽  
Author(s):  
Hongliang Zhu ◽  
Fuqu Hu ◽  
Ronghui Wang ◽  
Xin Zhou ◽  
Sing-Hoi Sze ◽  
...  
Keyword(s):  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Meristem Development

scholarly journals Spatiotemporal Sequestration of miR165/166 by Arabidopsis Argonaute10 Promotes Shoot Apical Meristem Maintenance

Cell Reports ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. 1819-1827 ◽  
Author(s):  
Yuyi Zhou ◽  
Minami Honda ◽  
Hongliang Zhu ◽  
Zhonghui Zhang ◽  
Xinwei Guo ◽  
...  
Keyword(s):  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Meristem Maintenance

Phyllotaxis of the palm Euterpe oleracea Mart. at the level of the shoot apical meristem

Botany ◽  
2010 ◽  
Vol 88 (5) ◽  
pp. 528-536 ◽  
Author(s):  
Denis Barabé ◽  
Laura Bourque ◽  
Xiaofeng Yin ◽  
Christian Lacroix
Keyword(s):  
Shoot Apical Meristem ◽  
Fundamental Theorem ◽  
Apical Meristem ◽  
Leaf Primordia ◽  
Leaf Primordium ◽  
Divergence Angle ◽  
Euterpe Oleracea ◽  
The Mean ◽  
The Relationship ◽  
Euterpe Oleracea Mart

Previous studies on palm phyllotaxis deal mainly with the mature trunk. The goals of this study are (i) to determine the relationship between the number of parastichies, the divergence angle, and the plastochrone ratio at the level of the shoot apical meristem; (ii) to examine whether there are fluctuations in the divergence angle; (iii) to interpret the significance of phyllotactic parameters with respect to the mode of growth of the apex. The tubular base of the leaf primordium is more or less asymmetrical, and completely surrounds the shoot apical meristem. The phyllotactic system corresponds to a (2, 3) conspicuous parastichy pair. The mean divergence angle per apex varies between 126.9° ± 9.3° (mean ± SD) and 135. 8° ± 8.0°. Divergence angles for all apices fluctuate within a range of 115.89° to 157.33°. The mean plastochrone ratios between apices varies from 1.35 ± 0.18 to 1.58 ± 0.12. The plastochrone ratio at each plastochrone for all apices ranges from 1.09 to 2.00. There is no correlation between the angle of divergence and the plastochrone ratio. There is a fluctuation in the value of the divergence angle that falls within the range predicted by the fundamental theorem of phyllotaxis. The high value of the ratio of the diameter of leaf primordia over the diameter of the apex, and the long plastochrone might explain the lack of correlation between certain phyllotactic parameters.

Sign in / Sign up

Export Citation Format

Share Document