Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

Development ◽  
1999 ◽  
Vol 126 (18) ◽  
pp. 4117-4128 ◽  
Author(s):  
K.R. Siegfried ◽  
Y. Eshed ◽  
S.F. Baum ◽  
D. Otsuga ◽  
G.N. Drews ◽  
...  
Keyword(s):  
Gene Family ◽  
Cell Fate ◽  
Apical Meristem ◽  
Ectopic Expression ◽  
Cell Fates ◽  
Lateral Organs ◽  
Expression Studies ◽  
Lateral Organ ◽  
Yabby Gene ◽  
Filamentous Flower

Lateral organs produced by shoot apical and flower meristems exhibit a fundamental abaxial-adaxial asymmetry. We describe three members of the YABBY gene family, FILAMENTOUS FLOWER, YABBY2 and YABBY3, isolated on the basis of homology to CRABS CLAW. Each of these genes is expressed in a polar manner in all lateral organ primordia produced from the apical and flower meristems. The expression of these genes is precisely correlated with abaxial cell fate in mutants in which abaxial cell fates are found ectopically, reduced or eliminated. Ectopic expression of either FILAMENTOUS FLOWER or YABBY3 is sufficient to specify the development of ectopic abaxial tissues in lateral organs. Conversely, loss of polar expression of these two genes results in a loss of polar differentiation of tissues in lateral organs. Taken together, these observations indicate that members of this gene family are responsible for the specification of abaxial cell fate in lateral organs of Arabidopsis. Furthermore, ectopic expression studies suggest that ubiquitous abaxial cell fate and maintenance of a functional apical meristem are incompatible.

scholarly journals Genome-wide identification and analysis of the YABBY gene family in Moso Bamboo (Phyllostachys edulis (Carrière) J. Houz)

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11780
Author(s):  
Ruifang Ma ◽  
Bin Huang ◽  
Zhinuo Huang ◽  
Zhijun Zhang
Keyword(s):  
Gene Family ◽  
Expression Patterns ◽  
Moso Bamboo ◽  
Lateral Organs ◽  
Genome Wide ◽  
Yabby Gene ◽  
Resistance To Stress ◽  
Hormone Responses ◽  
High Level

Background The YABBY gene family is a family of small zinc finger transcription factors associated with plant morphogenesis, growth, and development. In particular, it is closely related to the development of polarity in the lateral organs of plants. Despite being studied extensively in many plant species, there is little information on genome-wide characterization of this gene family in Moso bamboo. Methods In the present study, we identified 16 PeYABBY genes, which were unequally distributed on 11 chromosomes, through genome-wide analysis of high-quality genome sequences of M oso bamboo by bioinformatics tools and biotechnological tools. Gene expression under hormone stress conditions was verified by quantitative real-time PCR (qRT-PCR) experiments. Results Based on peptide sequences and similarity of exon-intron structures, we classified the PeYABBY genes into four subfamilies. Analysis of putative cis-acting elements in promoters of these genes revealed that PeYABBYs contained a large number of hormone-responsive and stress-responsive elements. Expression analysis showed that they were expressed at a high level in Moso bamboo panicles, rhizomes, and leaves. Expression patterns of putative PeYABBY genes in different organs and hormone-treated were analyzed using RNA-seq data, results showed that some PeYABBY genes were responsive to gibberellin (GA) and abscisic acid (ABA), indicating that they may play an important role in plant hormone responses. Gene Ontology (GO) analyses of YABBY proteins indicated that they may be involved in many developmental processes, particularly high level of enrichment seen in plant leaf development. In summary, our results provide a comprehensive genome-wide study of the YABBY gene family in bamboos, which could be useful for further detailed studies of the function and evolution of the YABBY genes, and to provide a fundamental basis for the study of YABBY in Gramineae for resistance to stress and hormonal stress.

scholarly journals The WUSCHELa (PtoWUSa) is Involved in Developmental Plasticity of Adventitious Root in Poplar

Genes ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 176 ◽  
Author(s):  
Jianbo Li ◽  
Huixia Jia ◽  
Pei Sun ◽  
Jin Zhang ◽  
Yongxiu Xia ◽  
...  
Keyword(s):  
Cell Fate ◽  
Apical Meristem ◽  
Developmental Plasticity ◽  
Profile Analysis ◽  
Primary Root ◽  
Ectopic Expression ◽  
Populus Tomentosa ◽  
Root Tip ◽  
Expression Profile Analysis ◽  
Auxin Transporter

WUSCHEL-RELATED HOMEOBOX (WOX) transcription factors play critical roles in cell fate determination during plant development. As the founding member of the WOX family, WUSCHEL (WUS) is characterized for its role in maintaining stem cell in meristem. In this study, we investigated the function of Populus tomentosa WUSCHELa (PtoWUSa) in adventitious roots (ARs) in poplar. Expression profile analysis showed that PtoWUSa was not only expressed in shoot apical meristem and stem, but also expressed in ARs. Ectopic expression of PtoWUSa in Arabidopsis resulted in shortened primary root, as well as agravitropism and multiple branches. Overexpression of PtoWUSa in poplar increased the number of ARs but decreased their length. Moreover, the AR tip and lateral root tip became larger and swollen. In addition, the expression of auxin transporter genes PIN-FORMED were downregulated in ARs of transgenic plant. Taken together, these results suggest that PtoWUSa could be involved in AR development in poplar through regulating the polar auxin transport in ARs.

scholarly journals Genome-Wide Identification of the YABBY Gene Family in Seven Species of Magnoliids and Expression Analysis in Litsea

Plants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 21
Author(s):  
Xuedie Liu ◽  
Xing-Yu Liao ◽  
Yu Zheng ◽  
Meng-Jia Zhu ◽  
Xia Yu ◽  
...  
Keyword(s):  
Gene Family ◽  
Developmental Stages ◽  
Gene Family Evolution ◽  
Protein Structure Analysis ◽  
Reading Frame ◽  
Lateral Organs ◽  
Genome Wide ◽  
Spatial Level ◽  
Yabby Gene ◽  
The Common

The YABBY gene family, specific to seed plants, encodes a class of transcription factors in the lamina maintenance and development of lateral organs. Magnoliids are sisters to the clade-containing eudicots and monocots, which have rapidly diversified among the common ancestors of these three lineages. However, prior to this study, information on the function of the YABBY genes in magnoliids was extremely limited to the third major clades and the early diverging lineage of Mesangiospermae. In this study, the sum of 55 YABBY genes including five genes in INO, six in CRC, eight in YAB2, 22 in YAB5, and 14 in FIL clade were identified from seven magnoliid plants. Sequence analysis showed that all encoded YABBY protein sequences possess the highly conserved YABBY domain and C2C2 zinc-finger domain. Gene and protein structure analysis indicates that a certain number of exons were highly conserved and similar in the same class, and YABBY genes encode proteins of 71–392 amino acids and an open reading frame of 216–1179 bp in magnoliids. Additionally, the predicted molecular weight and isoelectric point of YABBY proteins in three species ranged from 7689.93 to 43578.13 and from 5.33 to 9.87, respectively. Meanwhile, the YABBY gene homolog expression of Litsea was detected at a temporal and spatial level during various developmental stages of leaf and reproductive tissues. This research could provide a brief overview of YABBY gene family evolution and its differential expression in magnoliids. Therefore, this comprehensive diversification analysis would provide a new insight into further understanding of the function of genes in seven magnoliids.

scholarly journals Overexpression of a Pak Choi Gene, BcAS2, Causes Leaf Curvature in Arabidopsis thaliana

Genes ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 102
Author(s):  
Ye Lin ◽  
Hualan Hou ◽  
Yuhang Zhang ◽  
Xilin Hou
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Fate ◽  
Special Role ◽  
Amino Acid Residues ◽  
Pak Choi ◽  
Homologous Genes ◽  
Yeast Two Hybrid System ◽  
Lateral Organs ◽  
Lateral Organ ◽  
Lateral Organization

The LBD (Lateral Organ Boundaries Domain) family are a new group of plant-specific genes, which encode a class of transcription factors containing conserved Lateral Organization Boundary (LOB) domains, and play an important role in regulating the adaxial–abaxial polarity of plant leaves. In Arabidopsis thaliana, ASYMMETRIC LEAVES 2 (AS2) has a typical LOB domain and is involved in determining the adaxial cell fate. In this study, we isolated the BcAS2 gene from the pak choi cultivar “NHCC001”, and analyzed its expression pattern. The results showed that the BcAS2 encoded a protein made up of 202 amino acid residues which were located in the nucleus and cytomembrane. The Yeast two-hybrid system (Y2H) assay indicated that BcAS2 interacts with BcAS1-1 and BcAS1-2 (the homologous genes of AS1 gene in pak choi). In the transgenic Arabidopsis thaliana that overexpressed BcAS2 gene, it presented an abnormal phenotype with a curly shape. Taken together, our findings not only validate the function of BcAS2 in leaf development in Arabidopsis thaliana, but also contribute in unravelling the molecular regulatory mechanism of BcAS2, which fulfills a special role by forming complexes with BcAS1-1/2 in the establishment of the adaxial–abaxial polarity of the lateral organs in pak choi.

scholarly journals LsaYAB7, A Homologous Gene of FILAMENTOUS FLOWER, Participating In The Regulation of Adaxial-Abaxial Polarity of Leaves In Lettuce

2021 ◽  
Author(s):  
Kangsheng Luo ◽  
Dechun Zhang ◽  
Zhaodong Zhai ◽  
Xue Liu ◽  
Jun Zhou ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Gene Family ◽  
Ectopic Expression ◽  
Homologous Gene ◽  
Abiotic Stress Response ◽  
Chromosomal Distribution ◽  
Lateral Organs ◽  
Localization Analysis ◽  
Transcriptional Elements ◽  
Curled Leaves

Abstract Background: The plant-specific YABBY transcription factor plays important roles in the development of lateral organs, the establishment of adaxial-abaxial polarity and abiotic stress response. However, the function of YABBY gene family in lettuce (Lactuca sativa), an important leaf vegetable is still unclear yet. Results: In this study, we analyzed the chromosomal distribution, gene structure and cis-transcriptional elements of the YABBY gene family in lettuce. Phylogenetic analysis of YABBY genes in lettuce, Arabidopsis, maize and rice classified them into five groups. In addition, the expression profile of YABBY genes in lettuce indicated that they may play distinct functions in different tissues and developmental processes. The subcellular localization analysis and transactivation assay showed that LsaYAB7 probably functions as a transcription factor in the nucleus. Furthermore, the ectopic expression of LsaYAB7 exhibited abaxially curled leaves, not only in Arabidopsis, but also in lettuce. Conclusion: Altogether, LsaYAB7 is a key functional gene in determining the adaxial-abaxial polarity of lettuce leaves. Our study laid foundation for the molecular research of functional genes in lettuce.

scholarly journals CtBP impedes JNK- and Upd/STAT-driven cell fate misspecifications in regenerating Drosophila imaginal discs

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Melanie I Worley ◽  
Larissa A Alexander ◽  
Iswar K Hariharan
Keyword(s):  
Cell Fate ◽  
Tissue Damage ◽  
Binding Protein ◽  
Ectopic Expression ◽  
Cell Types ◽  
Imaginal Discs ◽  
Cell Fates ◽  
Genetic Ablation ◽  
Compartment Boundary ◽  
Wing Pouch

Regeneration following tissue damage often necessitates a mechanism for cellular re-programming, so that surviving cells can give rise to all cell types originally found in the damaged tissue. This process, if unchecked, can also generate cell types that are inappropriate for a given location. We conducted a screen for genes that negatively regulate the frequency of notum-to-wing transformations following genetic ablation and regeneration of the wing pouch, from which we identified mutations in the transcriptional co-repressor C-terminal Binding Protein (CtBP). When CtBP function is reduced, ablation of the pouch can activate the JNK/AP-1 and JAK/STAT pathways in the notum to destabilize cell fates. Ectopic expression of Wingless and Dilp8 precede the formation of the ectopic pouch, which is subsequently generated by recruitment of both anterior and posterior cells near the compartment boundary. Thus, CtBP stabilizes cell fates following damage by opposing the destabilizing effects of the JNK/AP-1 and JAK/STAT pathways.

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