Waste Soybean Oil and Corn Steep Liquor as Economic Substrates for Bioemulsifier and Biodiesel Production by Candida lipolytica UCP 0998

The study proposed the innovative low-cost strategy for erythritol production by Yarrowia lipolytica through developing a simple medium based on industrial waste by-products and a natural method for culture broth purification. Results obtained proved that corn steep liquor might successfully replace traditional sources of nitrogen and other nutrients without compromising activities of the enzymes responsible for erythritol production and its production level. As a consequence, a production process was performed where Y. lipolytica A-6 was able to produce 108.0 g/L of erythritol, with a production rate of 1.04 g/Lh and a yield of 0.45 g/g of the medium containing exclusively 220 g/L of crude glycerol derived from biodiesel production and 40 g/L of corn steep liquor. Moreover, a comparable concentration of erythritol (108.1 g/L) was obtained when a part of crude glycerol was exchanged for the crude fraction of fatty acids in the two-steps process. Next, the collected post-fermentation broths were used in the culture with Y. lipolytica Wratislavia K1 for natural purification. The process resulted in a high increase of erythritol selectivity from 72% to 97% and in the production of 22.0 g/L of biomass with 40.4% protein content, which enables its use as an attractive animal feedstuff.

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Production of a Biosurfactant by Cunninghamella echinulata Using Renewable Substrates and Its Applications in Enhanced Oil Spill Recovery

Colloids and Interfaces ◽

10.3390/colloids2040063 ◽

2018 ◽

Vol 2 (4) ◽

pp. 63 ◽

Cited By ~ 5

Author(s):

Patrícia de Souza ◽

Nadielly Andrade Silva ◽

Daniele Souza ◽

Thayse Lima e Silva ◽

Marta Freitas-Silva ◽

...

Keyword(s):

Surface Tension ◽

Soybean Oil ◽

Corn Steep Liquor ◽

Artemia Salina ◽

Engine Oil ◽

Variable Response ◽

Cunninghamella Echinulata ◽

Marine Sand ◽

Wide Range ◽

Steep Liquor

This study aimed to evaluate the production of a surfactant by Cunninghamella echinulata, using agro-industrial residues, corn steep liquor (CSL), and soybean oil waste (SOW). The study had a factorial design, using as a variable response to the reduction of surface tension. C. echinulata was able to produce biosurfactant in assay, CSL (8.82%) and SOW (2%). The results showed that the biosurfactant was successfully produced by C. echinulata and had attractive properties, such as a low surface tension (31.7 mN/m), a yield of 5.18 g/L at 120 h of cultivation, and an anionic profile. It also achieved a reduction in surface tension stability in a wide range of pH values, temperatures, and salinity values. The biosurfactant produced by C. echinulata showed an absence of toxicity to Artemia salina. The influence of the biosurfactant on the viscosity of engine oil, burnt engine oil, diesel, soybean oil post-frying, canola oil, and water was investigated. The results reveal a mechanism for the decrease of the viscosity using hydrophobic substrates and the new biosurfactant solution at 1.5% of the (CMC). This enables the formulation of a low-cost culture medium alternative, based on corn steep liquor and the reuse of soybean oil after frying to produce a biosurfactant. Additionally, performance of the biosurfactant isolated from C. echinulata showed an excellent ability to remove spilled oil, such as diesel (98.7%) and kerosene (92.3%) from marine sand.

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Improvement of lipase production from Geotrichum sp. in shaken flasks

Chemical Industry and Chemical Engineering Quarterly ◽

10.2298/ciceq120124022m ◽

2012 ◽

Vol 18 (3) ◽

pp. 459-464 ◽

Cited By ~ 5

Author(s):

Resende Maldonadoa ◽

Luiz Pancieraa ◽

Alves Macedob ◽

Marcio Mazuttic ◽

Francisco Maugeria ◽

...

Keyword(s):

Soybean Oil ◽

Lipase Activity ◽

Corn Steep Liquor ◽

Lipase Production ◽

Inoculum Size ◽

Inoculum Age ◽

Initial Ph ◽

Oil Concentration ◽

Inoculum Volume ◽

Steep Liquor

This work is focused on the study of different variables on inoculum build-up aiming to improve the lipase production by Geotrichum sp. by means a sequential strategy of experimental design. The effects of inoculum size, corn steep liquor concentration, volume of inoculum, pH of medium, age of inoculum and soybean oil concentration on lipase activity were assessed by means of two factorial experimental designs. A maximum lipase activity of 35.20?0.8 U/mL was obtained with a inoculum composed of one circular area of 0.78cm2 containing spores, 50 mL of inoculum volume medium, 12 hours of inoculum age, 15% w/v of corn steep liquor concentration, 1.0%w/v of soybean oil concentration and initial pH 5.0 at 30?C and 150 rpm in flasks. This work showed that an enhancement of lipase activity can be obtained using a sequential statistical factorial approach to define the variables for inoculum build-up.

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Synthesis and evaluation of biosurfactant produced by Candida lipolytica using animal fat and corn steep liquor

Journal of Petroleum Science and Engineering ◽

10.1016/j.petrol.2013.03.028 ◽

2013 ◽

Vol 105 ◽

pp. 43-50 ◽

Cited By ~ 69

Author(s):

Danyelle K.F. Santos ◽

Raquel D. Rufino ◽

Juliana M. Luna ◽

Valdemir A. Santos ◽

Alexandra A. Salgueiro ◽

...

Keyword(s):

Corn Steep Liquor ◽

Candida Lipolytica ◽

Animal Fat ◽

Steep Liquor

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Supplemental Corn Steep Liquor, Soap Stock and Soybean Oil for Finishing Beef Steers

Journal of Animal Science ◽

10.2527/jas1976.4241002x ◽

1976 ◽

Vol 42 (4) ◽

pp. 1002-1007 ◽

Cited By ~ 4

Author(s):

T. W. Perry ◽

W. H. Weatherly

Keyword(s):

Soybean Oil ◽

Soap Stock ◽

Corn Steep Liquor ◽

Beef Steers ◽

Steep Liquor

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Biodiesel production from waste soybean oil using jordanian oil shale ash

2010 1st International Nuclear & Renewable Energy Conference (INREC) ◽

10.1109/inrec.2010.5462594 ◽

2010 ◽

Author(s):

Mamdouh Allawzi ◽

Awni Al-Otoom ◽

Abdulaziz Ajlouni ◽

Fahmi Abu-Alrub ◽

Munther Qandah

Keyword(s):

Soybean Oil ◽

Oil Shale ◽

Biodiesel Production ◽

Oil Shale Ash ◽

Shale Ash ◽

Waste Soybean Oil

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Biodiesel production by enzymatic process using Jatropha oil and waste soybean oil

Biotechnology and Bioprocess Engineering ◽

10.1007/s12257-012-0805-8 ◽

2013 ◽

Vol 18 (4) ◽

pp. 703-708 ◽

Cited By ~ 18

Author(s):

Ja Hyun Lee ◽

Sung Bong Kim ◽

Hah Young Yoo ◽

Young Joon Suh ◽

Gyung Bo Kang ◽

...

Keyword(s):

Soybean Oil ◽

Biodiesel Production ◽

Jatropha Oil ◽

Enzymatic Process ◽

Waste Soybean Oil

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Continuous Biodiesel Production from Waste Soybean Oil Using a Nano-Fe3O4 Microwave Catalysis

Processes ◽

10.3390/pr9050756 ◽

2021 ◽

Vol 9 (5) ◽

pp. 756

Author(s):

Ching-Hsing Lin ◽

Yi-Tang Chang ◽

Mei-Chou Lai ◽

Tai-Ying Chiou ◽

Chien-Sen Liao

Keyword(s):

Soybean Oil ◽

Biodiesel Production ◽

Conventional Heating ◽

Molar Ratio ◽

Co Catalyst ◽

Heterogeneous Catalytic ◽

Nano Fe3o4 ◽

High Level ◽

Astm D6751 ◽

Waste Soybean Oil

In this study, we conducted an efficient microwave-assisted transesterification process combining homogeneous and heterogeneous catalytic phases to produce biodiesel from waste soybean oil. A cylindrical quartz reactor packed with nanoparticles of Fe3O4 as a co-catalyst was applied to improve the reaction. The process was carried out with a methanol-to-oil molar ratio of 6:1, power of 560 W, and residence time of 30 s. The specifications of the biodiesel produced in this study were compared with two standards, i.e., ASTM D6751 and EN 14214. We found that the continuous conversion of waste soybean oil to methyl ester was approximately 95%. The biodiesel showed a higher flash point and a higher carbon residue content than that of both standards, and the viscosity (5.356 mm2/s) and density (898.1 kg/m3) were both at a high level. Compared to a conventional heating plate, the energy consumption was significantly reduced by nearly 93%. It is expected that these findings will provide useful information for green and sustainable processes for the regeneration and reuse of oil.

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USE OF NORTHERN ECOTYPE SOYBEANS FOR BIOFUEL PRODUCTION

AGRICULTURAL ENGINEERING ◽

10.26897/2687-1149-2020-6-22-30 ◽

2020 ◽

pp. 22-30

Author(s):

SERGEY N. DEVYANIN ◽

◽

VLADIMIR A. MARKOV ◽

ALEKSANDR G. LEVSHIN ◽

TAMARA P. KOBOZEVA ◽

...

Keyword(s):

Diesel Engine ◽

Soybean Oil ◽

Diesel Fuel ◽

Experimental Studies ◽

Motor Fuel ◽

Biodiesel Production ◽

Biofuel Production ◽

Exhaust Gases ◽

Oil Productivity ◽

Toxic Components

The paper presents the results of long-term research on the oil productivity and chemical composition of soybean oil of the Northern ecotype varieties in the Central Non-Black Earth Region. The authors consider its possible use for biodiesel production. Experiments on growing soybeans were carried out on the experimental fi eld of Russian State Agrarian University –Moscow Timiryazev Agricultural Academy (2008-2019) on recognized ultra-early ripening varieties of the Northern ecotype Mageva, Svetlaya, Okskaya (ripeness group 000). Tests were set and the research results were analyzed using standard approved methods. It has been shown that in conditions of high latitudes (57°N), limited thermal resources of the Non-Chernozem zone of Russia (the sum of active temperatures of the growing season not exceeding 2000°С), the yield and productivity of soybeans depend on the variety and moisture supply. Over the years, the average yield of soybeans amounted to 1.94 … 2.62 t/ha, oil productivity – 388 … 544 kg/ha, oil content – 19…20%, the content of oleic and linoleic fatty acids in oil – 60%, and their output from seeds harvested – 300 kg/ha. It has been established that as soybean oil and diesel fuel have similar properties,they can be mixed by conventional methods in any proportions and form stable blends that can be stored for a long time. Experimental studies on the use of soybean oil for biodiesel production were carried out on a D-245 diesel engine (4 ChN11/12.5). The concentrations of toxic components (CO, CHx, and NOx) in the diesel exhaust gases were determined using the SAE-7532 gas analyzer. The smoke content of the exhaust gases was measured with an MK-3 Hartridge opacimeter. It has been experimentally established that the transfer of a diesel engine from diesel fuel to a blend of 80% diesel fuel and 20% lubrication oil leads to a change in the integral emissions per test cycle: nitrogen oxides in 0.81 times, carbon monoxide in 0.89 times and unburned hydrocarbons in 0.91 times, i.e. when biodiesel as used as a motor fuel in a serial diesel engine, emissions of all gaseous toxic components are reduced. The study has confi rmed the expediency of using soybeans of the Northern ecotype for biofuel production.

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