scholarly journals Production of a Biosurfactant by Cunninghamella echinulata Using Renewable Substrates and Its Applications in Enhanced Oil Spill Recovery

2018 ◽  
Vol 2 (4) ◽  
pp. 63 ◽  
Author(s):  
Patrícia de Souza ◽  
Nadielly Andrade Silva ◽  
Daniele Souza ◽  
Thayse Lima e Silva ◽  
Marta Freitas-Silva ◽  
...  
Keyword(s):  
Surface Tension ◽  
Soybean Oil ◽  
Corn Steep Liquor ◽  
Artemia Salina ◽  
Engine Oil ◽  
Variable Response ◽  
Cunninghamella Echinulata ◽  
Marine Sand ◽  
Wide Range ◽  
Steep Liquor

This study aimed to evaluate the production of a surfactant by Cunninghamella echinulata, using agro-industrial residues, corn steep liquor (CSL), and soybean oil waste (SOW). The study had a factorial design, using as a variable response to the reduction of surface tension. C. echinulata was able to produce biosurfactant in assay, CSL (8.82%) and SOW (2%). The results showed that the biosurfactant was successfully produced by C. echinulata and had attractive properties, such as a low surface tension (31.7 mN/m), a yield of 5.18 g/L at 120 h of cultivation, and an anionic profile. It also achieved a reduction in surface tension stability in a wide range of pH values, temperatures, and salinity values. The biosurfactant produced by C. echinulata showed an absence of toxicity to Artemia salina. The influence of the biosurfactant on the viscosity of engine oil, burnt engine oil, diesel, soybean oil post-frying, canola oil, and water was investigated. The results reveal a mechanism for the decrease of the viscosity using hydrophobic substrates and the new biosurfactant solution at 1.5% of the (CMC). This enables the formulation of a low-cost culture medium alternative, based on corn steep liquor and the reuse of soybean oil after frying to produce a biosurfactant. Additionally, performance of the biosurfactant isolated from C. echinulata showed an excellent ability to remove spilled oil, such as diesel (98.7%) and kerosene (92.3%) from marine sand.

scholarly journals Eco-Friendly Bioemulsifier Production by Mucor circinelloides UCP0001 Isolated from Mangrove Sediments Using Renewable Substrates for Environmental Applications

Biomolecules ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 365 ◽  
Author(s):  
Nathália S. A. A. Marques ◽  
Israel G. Sales da Silva ◽  
Davi L. Cavalcanti ◽  
Patrícia C. S. V. Maia ◽  
Vanessa P. Santos ◽  
...  
Keyword(s):  
Canola Oil ◽  
Raw Materials ◽  
Corn Steep Liquor ◽  
Mucor Circinelloides ◽  
Artemia Salina ◽  
Engine Oil ◽  
Extreme Conditions ◽  
Linear Quadratic ◽  
Statistical Validation ◽  
Steep Liquor

The successful production of a biosurfactant is dependent on the development of processes using low cost raw materials. In the present work, an economically attractive medium composed of corn steep liquor and waste cooking oil was formulated to maximize the production of bioemulsifier by Mucor circinelloides UCP0001. A central rotational composite design was applied to statistical validation of the production. The emulsifying properties, stability under extreme conditions, its toxicity character, and the characterization of the bioemulsifier were determined. The best condition for biomolecule synthesis occurred in the assay 2 containing 4% of corn steep liquor and 3% waste soybean oil and exhibited 100% emulsification index for canola oil and petroleum, as well as excellent emulsifying activity for canola oil and burned engine oil. The nutritional factors studied showed statistical relevance, since all linear, quadratic effects and their interactions were significant. The bioemulsifier showed 2.69 g/L yield and the chemical character of the molecule structure was identified by FT-IR (Fourier Transform Infrared) spectroscopy. The bioemulsifier showed no toxicity to Artemia salina and Chlorella vulgaris. Stable emulsions were obtained under extreme conditions of temperature, pH, and salinity. These findings contribute to understanding of the relationship between production, physical properties, chemical composition, and stability of bioemulsifier for their potential applications in biotechnology, such as bioremediation of hydrocarbon-contaminated soil and water.

scholarly journals Promising Biosurfactant Produced byCunninghamella echinulataUCP 1299 Using Renewable Resources and Its Application in Cotton Fabric Cleaning Process

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Rosileide F. S. Andrade ◽  
Thayse A. L. Silva ◽  
Daylin R. Ribeaux ◽  
Dayana M. Rodriguez ◽  
Adriana F. Souza ◽  
...  
Keyword(s):  
Cotton Fabric ◽  
Automobile Industry ◽  
Structural Integrity ◽  
Corn Steep Liquor ◽  
Engine Oil ◽  
Nmr Spectrum ◽  
Hydrophobic Residues ◽  
Carbon Nitrogen Ratio ◽  
Nitrogen Ratio ◽  
Steep Liquor

A biosurfactant was produced fromCunninghamella echinulatausing sustainable technology for cleaning and degreasing of cotton fabric impregnated with burned motor oil. The surface tension was 32.4 mN/m on a medium containing instant noodle waste (2%), corn steep liquor (2%), and postfrying oil (0.5%) with a carbon/nitrogen ratio of 30 : 1, yield of 6.0 g·L−1, emulsifier index of 81.4%, and dispersant property of 32.15 cm2. The biosurfactant produced is a glycolipid constituted by carbohydrate (47.7%) and lipids (50.0%). The structure was confirmed by GC-MS (stearic acid in predominance with mass of 298 m/z), FTIR spectroscopy (polysaccharides in bands between 1025 and 1152 cm−1and fatty acids in bands between 2057 and 3100 cm−1), 1H NMR, and 13C NMR spectrum (carbohydrates in signal of 4.38 ppm and 77.0 ppm). The properties of cleaning and degreasing of burned engine oil in cotton fabric by biosurfactant ofC. echinulatawas evidenced by removal of 86% of oil. After use of the biosurfactant, the fibers were not damaged, which is important for structural integrity of cotton fabric after the wash. In addition, the biosurfactant did not show toxic effect. This study suggests that the biosurfactant fromC. echinulatacan be used in formulation of textile detergents, in particular for removal of hydrophobic residues from the automobile industry.

scholarly journals Waste Soybean Oil and Corn Steep Liquor as Economic Substrates for Bioemulsifier and Biodiesel Production by Candida lipolytica UCP 0998

2016 ◽  
Vol 17 (10) ◽  
pp. 1608 ◽  
Author(s):  
Adriana Souza ◽  
Dayana Rodriguez ◽  
Daylin Ribeaux ◽  
Marcos Luna ◽  
Thayse Lima e Silva ◽  
...  
Keyword(s):  
Soybean Oil ◽  
Corn Steep Liquor ◽  
Biodiesel Production ◽  
Candida Lipolytica ◽  
Steep Liquor ◽  
Waste Soybean Oil

scholarly journals Improvement of lipase production from Geotrichum sp. in shaken flasks

2012 ◽  
Vol 18 (3) ◽  
pp. 459-464 ◽  
Author(s):  
Resende Maldonadoa ◽  
Luiz Pancieraa ◽  
Alves Macedob ◽  
Marcio Mazuttic ◽  
Francisco Maugeria ◽  
...  
Keyword(s):  
Soybean Oil ◽  
Lipase Activity ◽  
Corn Steep Liquor ◽  
Lipase Production ◽  
Inoculum Size ◽  
Inoculum Age ◽  
Initial Ph ◽  
Oil Concentration ◽  
Inoculum Volume ◽  
Steep Liquor

This work is focused on the study of different variables on inoculum build-up aiming to improve the lipase production by Geotrichum sp. by means a sequential strategy of experimental design. The effects of inoculum size, corn steep liquor concentration, volume of inoculum, pH of medium, age of inoculum and soybean oil concentration on lipase activity were assessed by means of two factorial experimental designs. A maximum lipase activity of 35.20?0.8 U/mL was obtained with a inoculum composed of one circular area of 0.78cm2 containing spores, 50 mL of inoculum volume medium, 12 hours of inoculum age, 15% w/v of corn steep liquor concentration, 1.0%w/v of soybean oil concentration and initial pH 5.0 at 30?C and 150 rpm in flasks. This work showed that an enhancement of lipase activity can be obtained using a sequential statistical factorial approach to define the variables for inoculum build-up.

Optimization of medium components to improve the antimicrobial activity of a new Saharan actinobacterial strain Saccharothrix tamanrassetensis DSM 45947

2021 ◽  
Vol 16 (7) ◽  
pp. 35-47
Author(s):  
Keramane Leila Bakour ◽  
Hadjira Boudjella ◽  
Nouredinne Bouras ◽  
Florence Mathieu
Keyword(s):  
Antimicrobial Activity ◽  
Antibiotic Production ◽  
Corn Steep Liquor ◽  
Basal Medium ◽  
Fermentation Medium ◽  
Antimicrobial Activities ◽  
Potential Candidate ◽  
Medium Components ◽  
Wide Range ◽  
Steep Liquor

Saccharothrix tamanrassetensis DSM 45947 previously isolated from a Saharan soil sample and characterized as a new species, was found to be a potential candidate in the search for novel antibiotics. The strain was found to exhibit a strong antimicrobial activity against a wide range of bacteria and fungi. In this study, statistical methods were applied to optimize the fermentation medium for enhanced antimicrobial activity production. A basal medium supplemented with sucrose and corn steep liquor was used as the original medium for optimization experiments. Plackett-Burman design showed that sucrose, corn steep liquor and KH2PO4, were recognised to have significant effect on antimicrobial activity production. Response surface methodology with Box-Behnken design determined the optimal concentrations. Optimal values for maximum antibiotic production were as follows: 7 g L-1 of sucrose, 52 g L-1 of corn steep liquor and 0.9 g L-1 of KH2PO4. Under these conditions, antimicrobial activities of DSM 45947 showed a global increase by 32% against the bacterium methicillinresistant Staphylococcus aureus and 36% against the fungus Umbelopsis ramanniana. This study provided an economical and efficient method to cultivate Saccharothrix tamanrassetensis DSM 45947 for enhanced antimicrobial activity production.

scholarly journals Production, Characterization and Commercial Formulation of a Biosurfactant from Candida tropicalis UCP0996 and Its Application in Decontamination of Petroleum Pollutants

Processes ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 885
Author(s):  
Darne Germano Almeida ◽  
Rita de Cássia Freire Soares da Silva ◽  
Hugo Morais Meira ◽  
Pedro Pinto Ferreira Brasileiro ◽  
Elias José Silva ◽  
...  
Keyword(s):  
Candida Tropicalis ◽  
Oil Spills ◽  
Low Cost ◽  
Corn Steep Liquor ◽  
Chemical Characterization ◽  
Artemia Salina ◽  
Motor Oil ◽  
The Stability ◽  
Steep Liquor ◽  
Layer Chromatography

Contamination by oil and its derivatives causes serious damage to the environment, motivating the development of innovative technologies for the removal of these contaminants, such as the use of biosurfactants. In the present study, the biosurfactant from Candida tropicalis UCP0996 produced in the low cost-medium formulated with molasses, residual frying oil, and corn steep liquor, was characterized and its toxicity, formulation, and application in removal and biodegradation of oil were investigated. The surface tension of the medium was reduced to 30.4 mN/m, yielding 4.11 g/L of isolated biosurfactant after 120 h. Tests under extreme environmental conditions indicated the stability of the biosurfactant. Chemical characterization by thin layer chromatography (TLC), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (1H NMR), and gas chromatography and mass spectroscopy (CG-MS) revealed the glycolipidic nature of the biosurfactant. The isolated biosurfactant showed no toxicity against the microcrustacean Artemia salina, while the properties of the formulated biosurfactant remained stable during 120 days of storage. The biosurfactant removed 66.18% of motor oil adsorbed in marine stones and dispersed 70.95% of oil in seawater. The biosurfactant was also able to increase by 70% the degradation of motor oil by seawater indigenous microorganisms, showing great potential to be applied as a commercial additive in the bioremediation of oil spills.

Application of response surface methodology and central composite rotatable design (CCDR) for modelling the influence of agro-industrial waste in lactic acid biosynthesis

2019 ◽  
Vol 6 (4) ◽  
pp. 51
Author(s):  
Ana Paula Testa Pezzin ◽  
Jaqueline Boldt Capellari ◽  
Eduarda Zeni Neves ◽  
Michele Cristina Formolo Garcia ◽  
Giannini Pasiznick Apati ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Experimental Design ◽  
Industrial Waste ◽  
Corn Steep Liquor ◽  
Microbial Culture ◽  
Central Composite Rotatable Design ◽  
Wide Range ◽  
Steep Liquor ◽  
Central Composite ◽  
Rotatable Design

Lactic acid (LA) is one of the most important organic acids, with a wide range of industrial and biotechnological applications and can be produced by chemical synthesis and microbial culture. However, the biotech pathway is generally preferred because it provides an optically pure product. In this context, the purpose of this work was to evaluate LA biosynthesis by Lactobacillus amylovorus using molasses as carbon source (CS) and corn steep liquor as nitrogen source (NS) in a central composite rotatable design (CCRD) varying the concentration CS and NS, as well as to validate the model. The method for microbial culture followed an experimental design of the CCRD type, conducted without agitation, at 37ºC in Erlenmeyer flask, with pH in spontaneous evolution. The results showed that, using molasses and corn steep liquor as alternative sources, LA production ranged from 2.8 to 4.6 g/L, respectively, with the most favourable condition being 40.0 g of molasses and 250 g of corn steep liquor. It was possible, from the experimental design, to ascertain the selection of the best conditions for the microbial culture, demonstrating the feasibility of replacing CS and NS by agro-industrial waste, thus reducing the cost of producing LA.

scholarly journals Effects of Organic Fertilizer on Bok Choy Growth and Quality in Hydroponic Cultures

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 491
Author(s):  
Kazuki Kano ◽  
Hiroaki Kitazawa ◽  
Keitaro Suzuki ◽  
Ani Widiastuti ◽  
Hiromitsu Odani ◽  
...  
Keyword(s):  
Plant Root ◽  
Oxygen Deficiency ◽  
Organic Fertilizer ◽  
Corn Steep Liquor ◽  
Ascorbic Acid Content ◽  
Growth And Yield ◽  
Significant Difference ◽  
Carbon Nitrogen Ratio ◽  
Nitrogen Ratio ◽  
Steep Liquor

Effects of corn steep liquor (organic fertilizer, OF) and conventional chemical fertilizer (CF) on the growth and yield of bok choy (Brassica rapa var. chinensis) in summer and autumn hydroponic growing systems were compared. When OF and CF were applied with the same amount of total nitrogen in summer cultivation, there was no significant difference between yields; however, the growth rate in OF was slower than in CF. When OF was applied with twice the amount of nitrogen in CF (OF2), bok choy growth and yield were significantly inhibited in summer cultivation, likely owing to dissolved oxygen deficiency and different rates of nitrification and nitrogen absorbance by the plant root. Although the contents of potassium, calcium, and magnesium in bok choy showed no difference among the three treatments in both cultivation seasons, the carbon/nitrogen ratio tended to be higher in OF and OF2 than in CF. Lower nitric acid and higher ascorbic acid content was found in OF and OF2 than in CF. Overall, our results suggest that a comparable yield is expected by using the same nitrogen amount with a conventional recipe of chemical fertilization in autumn cultivation. However, further improvement of hydroponic management is needed in summer cultivation.

scholarly journals Effects of corn steep liquor on β-poly(l-malic acid) production in Aureobasidium melanogenum

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Genan Wang ◽  
Bingyi Shi ◽  
Pan Zhang ◽  
Tingbin Zhao ◽  
Haisong Yin ◽  
...  
Keyword(s):  
Malic Acid ◽  
Low Cost ◽  
Corn Steep Liquor ◽  
Enrichment Analysis ◽  
Tca Cycle ◽  
Water Soluble ◽  
Growth And Yield ◽  
The Tca Cycle ◽  
Steep Liquor ◽  
Aureobasidium Melanogenum

Abstractβ-poly(l-malic acid) (PMLA) is a water-soluble biopolymer used in medicine, food, and other industries. However, the low level of PMLA biosynthesis in microorganisms limits its further application in the biotechnological industry. In this study, corn steep liquor (CSL), which processes high nutritional value and low-cost characteristics, was selected as a growth factor to increase the PMLA production in strain, Aureobasidium melanogenum, and its metabolomics change under the CSL addition was investigated. The results indicated that, with 3 g/L CSL, PMLA production, cell growth, and yield (Yp/x) were increased by 32.76%, 41.82%, and 47.43%, respectively. The intracellular metabolites of A. melanogenum, such as amino acids, organic acids, and key intermediates in the TCA cycle, increased after the addition of CSL, and the enrichment analysis showed that tyrosine may play a major role in the PMLA biosynthesis. The results presented in this study demonstrated that the addition of CSL would be an efficient approach to improve PMLA production.

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