scholarly journals Human Leukocyte Antigen and Red Blood Cells Impact Umbilical Cord Blood CD34+ Cell Viability after Thawing

2019 ◽  
Vol 20 (19) ◽  
pp. 4875 ◽  
Author(s):  
Vanegas ◽  
Galindo ◽  
Páez-Gutiérrez ◽  
González-Acero ◽  
Medina-Valderrama ◽  
...  
Keyword(s):  
Human Leukocyte Antigen ◽  
Cell Viability ◽  
Cord Blood ◽  
Red Blood Cells ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Blood Cells ◽  
Human Leukocyte ◽  
Hla Typing ◽  
Leukocyte Antigen

Hematopoietic progenitor cell (HPC) transplantation is a treatment option for malignant and nonmalignant diseases. Umbilical cord blood (UCB) is an important HPC source, mainly for pediatric patients. It has been demonstrated that human leukocyte antigen (HLA) matching and cell dose are the most important features impacting clinical outcomes. However, UCB matching is performed using low resolution HLA typing and it has been demonstrated that the unnoticed mismatches negatively impact the transplant. Since we found differences in CD34+ viability after thawing of UCB units matched for two different patients (p = 0.05), we presumed a possible association between CD34+ cell viability and HLA. We performed a multivariate linear model (n = 67), comprising pre-cryopreservation variables and high resolution HLA genotypes separately. We found that pre-cryopreservation red blood cells (RBC), granulocytes, and viable CD34+ cell count significantly impacted CD34+ viability after thawing, along with HLA-B or -C (R2 = 0.95, p = 0.01; R2 = 0.56, p = 0.007, respectively). Although HLA-B*40:02 may have a negative impact on CD34+ cell viability, RBC depletion significantly improves it.

Umbilical cord blood CD34+cell–derived progeny produces human leukocyte antigen–G molecules with immuno-modulatory functions

2012 ◽  
Vol 73 (2) ◽  
pp. 150-155 ◽  
Author(s):  
Marina Buzzi ◽  
Francesco Alviano ◽  
Diana Campioni ◽  
Marina Stignani ◽  
Loredana Melchiorri ◽  
...  
Keyword(s):  
Human Leukocyte Antigen ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Human Leukocyte ◽  
Leukocyte Antigen ◽  
Cord Blood Cd34 ◽  
Human Leukocyte Antigen G

P069 Next generation sequencing based polymorphism and gene expression analysis of umbilical cord blood human leukocyte antigen

2016 ◽  
Vol 77 ◽  
pp. 88
Author(s):  
Fiona F. Yamamoto ◽  
Dolly B. Tyan ◽  
Marcelo Fernandez-Viña ◽  
Shingo Suzuki ◽  
Takashi Shiina ◽  
...  
Keyword(s):  
Gene Expression ◽  
Next Generation Sequencing ◽  
Human Leukocyte Antigen ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Gene Expression Analysis ◽  
Human Leukocyte ◽  
Leukocyte Antigen ◽  
Generation Sequencing

Human leukocyte antigen (HLA)-G during pregnancy part I: Correlations between maternal soluble HLA-G at midterm, at term, and umbilical cord blood soluble HLA-G at term

2015 ◽  
Vol 76 (4) ◽  
pp. 254-259 ◽  
Author(s):  
Louise Klitkou ◽  
Mette Dahl ◽  
Thomas Vauvert F. Hviid ◽  
Snezana Djurisic ◽  
Zofia Maria Piosik ◽  
...  
Keyword(s):  
Human Leukocyte Antigen ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Human Leukocyte ◽  
Leukocyte Antigen ◽  
Soluble Hla

Potential use of autologous umbilical cord blood red blood cells for early transfusion needs of premature infants

Transfusion ◽  
2006 ◽  
Vol 46 (6) ◽  
pp. 1049-1056 ◽  
Author(s):  
Marijke Jansen ◽  
Anneke Brand ◽  
Jeannette S. von Lindern ◽  
Sicco Scherjon ◽  
Frans J. Walther
Keyword(s):  
Cord Blood ◽  
Red Blood Cells ◽  
Umbilical Cord ◽  
Premature Infants ◽  
Umbilical Cord Blood ◽  
Blood Cells ◽  
Potential Use

scholarly journals Mislabeled units of umbilical cord blood detected by a quality assurance program at the transplantation center

Blood ◽  
2009 ◽  
Vol 114 (8) ◽  
pp. 1684-1688 ◽  
Author(s):  
Jeffrey McCullough ◽  
David McKenna ◽  
Diane Kadidlo ◽  
David Maurer ◽  
Harriett J. Noreen ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Human Leukocyte ◽  
Hla Class I ◽  
Hla Typing ◽  
Class I ◽  
Quality Assurance Program ◽  
Cord Blood Unit ◽  
Transplantation Center

Abstract We instituted procedures to check the identity of cord blood unit provided for transplantation by carrying out ABO and human leukocyte antigen (HLA) typing of the thawed units before transplantation. ABO typing is done using standard techniques. Rapid HLA class I serology is with monoclonal antibody trays (One Lambda Inc) using standard incubations. One mislabeled umbilical cord blood (UCB) unit was detected on the day of intended transplantation by repeat ABO typing of the thawed unit at our transplantation center. Because ABO typing will not detect all labeling errors, the rapid serologic class I HLA typing procedure was done on thawed units just before transplantation for all units without an attached segment. This procedure identified a second mislabeled unit. In a 6-year period, 2 of 871 (0.2%) cord blood units sent to us for transplantation were mislabeled and potentially would have been transplanted incorrectly. This error rate of 1 per 249 (0.4%) patients could have potentially devastating consequences.

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