scholarly journals Integrating LCM-Based Spatio-Temporal Transcriptomics Uncovers Conceptus and Endometrial Luminal Epithelium Communication that Coordinates the Conceptus Attachment in Pigs

2021 ◽  
Vol 22 (3) ◽  
pp. 1248
Author(s):  
Feiyu Wang ◽  
Shilei Zhao ◽  
Dadong Deng ◽  
Weiwei Wang ◽  
Xuewen Xu ◽  
...  

Attachment of conceptus to the endometrial luminal epithelium (LE) is a critical event for early placentation in Eutheria. Since the attachment occurs at a particular site within the uterus, a coordinated communication between three spatially distinct compartments (conceptus and endometrial LE from two anatomical regions of the uterus to which conceptus attaches and does not attach) is essential but remains to be fully characterized. Using the laser capture microdissection (LCM) technique, we firstly developed an approach that can allow us to pair the pig conceptus sample with its nearby endometrial epithelium sample without losing the native spatial information. Then, a comprehensive spatio-temporal transcriptomic profile without losing the original conceptus-endometrium coordinates was constructed. The analysis shows that an apparent difference in transcriptional responses to the conceptus exists between the endometrial LE from the two anatomically distinct regions in the uterus. In addition, we identified the communication pathways that link the conceptus and endometrial LE and found that these pathways have important roles in conceptus attachment. Furthermore, a number of genes whose expression is spatially restricted in the two different anatomical regions within the uterus were characterized for the first time and two of them (SULT2A1 and MEP1B) may cooperatively contribute to establish conceptus attachment in pigs. The results from our study have implications in understanding of conceptus/embryo attachment in pigs and other large polytocous species.

2021 ◽  
Vol 10 (3) ◽  
pp. 166
Author(s):  
Hartmut Müller ◽  
Marije Louwsma

The Covid-19 pandemic put a heavy burden on member states in the European Union. To govern the pandemic, having access to reliable geo-information is key for monitoring the spatial distribution of the outbreak over time. This study aims to analyze the role of spatio-temporal information in governing the pandemic in the European Union and its member states. The European Nomenclature of Territorial Units for Statistics (NUTS) system and selected national dashboards from member states were assessed to analyze which spatio-temporal information was used, how the information was visualized and whether this changed over the course of the pandemic. Initially, member states focused on their own jurisdiction by creating national dashboards to monitor the pandemic. Information between member states was not aligned. Producing reliable data and timeliness reporting was problematic, just like selecting indictors to monitor the spatial distribution and intensity of the outbreak. Over the course of the pandemic, with more knowledge about the virus and its characteristics, interventions of member states to govern the outbreak were better aligned at the European level. However, further integration and alignment of public health data, statistical data and spatio-temporal data could provide even better information for governments and actors involved in managing the outbreak, both at national and supra-national level. The Infrastructure for Spatial Information in Europe (INSPIRE) initiative and the NUTS system provide a framework to guide future integration and extension of existing systems.


IEEE Access ◽  
2020 ◽  
Vol 8 ◽  
pp. 126965-126976
Author(s):  
Xiaoyu Cong ◽  
Yubing Han ◽  
Weixing Sheng ◽  
Shanhong Guo ◽  
Renli Zhang

Author(s):  
XIAN WU ◽  
JIANHUANG LAI ◽  
PONG C. YUEN

This paper proposes a novel approach for video-shot transition detection using spatio-temporal saliency. Both temporal and spatial information are combined to generate a saliency map, and features are available based on the change of saliency. Considering the context of shot changes, a statistical detector is constructed to determine all types of shot transitions by the minimization of the detection-error probability simultaneously under the same framework. The evaluation performed on videos of various content types demonstrates that the proposed approach outperforms a more recent method and two publicly available systems, namely VideoAnnex and VCM.


2001 ◽  
Vol 10 (04) ◽  
pp. 715-734 ◽  
Author(s):  
SHU-CHING CHEN ◽  
MEI-LING SHYU ◽  
CHENGCUI ZHANG ◽  
R. L. KASHYAP

The identification of the overlapped objects is a great challenge in object tracking and video data indexing. For this purpose, a backtrack-chain-updation split algorithm is proposed to assist an unsupervised video segmentation method called the "simultaneous partition and class parameter estimation" (SPCPE) algorithm to identify the overlapped objects in the video sequence. The backtrack-chain-updation split algorithm can identify the split segment (object) and use the information in the current frame to update the previous frames in a backtrack-chain manner. The split algorithm provides more accurate temporal and spatial information of the semantic objects so that the semantic objects can be indexed and modeled by multimedia input strings and the multimedia augmented transition network (MATN) model. The MATN model is based on the ATN model that has been used in artificial intelligence (AI) areas for natural language understanding systems, and its inputs are modeled by the multimedia input strings. In this paper, we will show that the SPCPE algorithm together with the backtrack-chain-updation split algorithm can significantly enhance the efficiency of spatio-temporal video indexing by improving the accuracy of multimedia database queries related to semantic objects.


Author(s):  
Paolo Corti ◽  
Benjamin G Lewis ◽  
Athanasios Tom Kralidis ◽  
Ntabathia Jude Mwenda

A Spatial Data Infrastructure (SDI) is a framework of geospatial data, metadata, users and tools intended to provide an efficient and flexible way to use spatial information. One of the key software components of an SDI is the catalogue service which is needed to discover, query, and manage the metadata. Catalogue services in an SDI are typically based on the Open Geospatial Consortium (OGC) Catalogue Service for the Web (CSW) standard which defines common interfaces for accessing the metadata information. A search engine is a software system capable of supporting fast and reliable search, which may use “any means necessary” to get users to the resources they need quickly and efficiently. These techniques may include features such as full text search, natural language processing, weighted results, fuzzy tolerance results, faceting, hit highlighting, recommendations, feedback mechanisms based on log mining, usage statistic gathering, and many others. In this paper we will be focusing on improving geospatial search with a search engine platform that uses Lucene, a Java-based search library, at its core. In work funded by the National Endowment for the Humanities, the Centre for Geographic Analysis (CGA) at Harvard University is in the process of re-engineering the search component of its public domain SDI (WorldMap http://worldmap.harvard.edu ) which is based on the GeoNode platform. In the process the CGA has developed Harvard Hypermap (HHypermap), a map services registry and search platform independent from WorldMap. The goal of HHypermap is to provide a framework for building and maintaining a comprehensive registry of web map services, and because such a registry is expected to be large, the system supports the development of clients with modern search capabilities such as spatial and temporal faceting and instant previews via an open API. Behind the scenes HHypermap scalably harvests OGC and Esri service metadata from distributed servers, organizes that information, and pushes it to a search engine. The system monitors services for reliability and uses that to improve search. End users will be able to search the SDI metadata using standard interfaces provided by the internal CSW catalogue, and will benefit from the enhanced search possibilities provided by an advanced search engine. HHypermap is built on an open source software source stack.


2016 ◽  
Author(s):  
Paolo Corti ◽  
Benjamin G Lewis ◽  
Tom Kralidis ◽  
Jude Mwenda

A Spatial Database Infrastructure (SDI) is a framework of geospatial data, metadata, users and tools intended to provide the most efficient and flexible way to use spatial information. One of the key software component of a SDI is the catalogue service, needed to discover, query and manage the metadata. Catalogue services in a SDI are typically based on the Open Geospatial Consortium (OGC) Catalogue Service for the Web (CSW) standard, that defines common interfaces to access the metadata information. A search engine is a software system able to perform very fast and reliable search, with features such as full text search, natural language processing, weighted results, fuzzy tolerance results, faceting, hit highlighting and many others. The Centre of Geographic Analysis (CGA) at Harvard University is trying to integrate within its public domain SDI (named WorldMap), the benefits of both worlds (OGC catalogs and search engines). Harvard Hypermap (HHypermap) is a component that will be part of WorldMap, totally built on an open source stack, implementing an OGC catalog, based on pycsw, to provide access to metadata in a standard way, and a search engine, based on Solr/Lucene, to provide the advanced search features typically found in search engines.


2018 ◽  
Vol 16 (7) ◽  
Author(s):  
Mimi Zaleha Abdul Ghani ◽  
Yazid Sarkom ◽  
Zalina Samadi

This paper aims to explore the rich potential of interactive visualisation environment integrating GIS for modelling urban growth and spatio-temporal transformation of Malaysian cities. As a case study example, authors consider a 3-D GIS model of Ampang Jaya, Selangor to investigate the techniques of data acquisition, data reconstruction from physical to digital, urban analysis and visualisation in constructing a digital model ranging from low to high geometric content including 2-D digital maps, digital orthographic and full volumetricparametric modelling. The key aspect of this virtual model is how it would assist in understanding the urban planning and design of Ampang Jaya by translating complex spatial information that are currently used by the authorities for planning activities such as maps, plans and written information into responsive, easily understandable spatial information. It could serve as a new platform to disseminate information about Ampang Jaya, bridge gaps among professionals involved in planning processes, improve communications among decision makers, stakeholders and the public as well as support decision making about thespatial growth of Ampang Jaya. Demonstrations of Ampang Jaya will also provide a clearer picture of the importance of ownership and control of 3-D models by local councils in empowering them in decision making, for example, in improving transparency, and avoiding misuse by project developers (Shiffer 1993; Sunesson et al., 2008). Such environment will improve the subsequent digital models and research in the area of urban design and planning in Malaysia where visual communication is pivotal.


2016 ◽  
Author(s):  
Paolo Corti ◽  
Benjamin G Lewis ◽  
Tom Kralidis ◽  
Jude Mwenda

A Spatial Data Infrastructure (SDI) is a framework of geospatial data, metadata, users and tools intended to provide the most efficient and flexible way to use spatial information. One of the key software components of a SDI is the catalogue service, needed to discover, query and manage the metadata. Catalogue services in a SDI are typically based on the Open Geospatial Consortium (OGC) Catalogue Service for the Web (CSW) standard, that defines common interfaces to access the metadata information. A search engine is a software system able to perform very fast and reliable search, with features such as full text search, natural language processing, weighted results, fuzzy tolerance results, faceting, hit highlighting and many others. The Centre of Geographic Analysis (CGA) at Harvard University is trying to integrate within its public domain SDI (named WorldMap), the benefits of both worlds (OGC catalogues and search engines). Harvard Hypermap (HHypermap) is a component that will be part of WorldMap, totally built on an open source stack, implementing an OGC catalogue, based on pycsw, to provide access to metadata in a standard way, and a search engine, based on Solr/Lucene, to provide the advanced search features typically found in search engines.


2016 ◽  
Author(s):  
Paolo Corti ◽  
Benjamin G Lewis ◽  
Tom Kralidis ◽  
Jude Mwenda

A Spatial Data Infrastructure (SDI) is a framework of geospatial data, metadata, users and tools intended to provide the most efficient and flexible way to use spatial information. One of the key software components of a SDI is the catalogue service, needed to discover, query and manage the metadata. Catalogue services in a SDI are typically based on the Open Geospatial Consortium (OGC) Catalogue Service for the Web (CSW) standard, that defines common interfaces to access the metadata information. A search engine is a software system able to perform very fast and reliable search, with features such as full text search, natural language processing, weighted results, fuzzy tolerance results, faceting, hit highlighting and many others. The Centre of Geographic Analysis (CGA) at Harvard University is trying to integrate within its public domain SDI (named WorldMap), the benefits of both worlds (OGC catalogues and search engines). Harvard Hypermap (HHypermap) is a component that will be part of WorldMap, totally built on an open source stack, implementing an OGC catalogue, based on pycsw, to provide access to metadata in a standard way, and a search engine, based on Solr/Lucene, to provide the advanced search features typically found in search engines.


2012 ◽  
Vol 5 (1) ◽  
pp. 1-66 ◽  
Author(s):  
R. Kumar ◽  
M. Naja ◽  
G. G. Pfister ◽  
M. C. Barth ◽  
C. Wiedinmyer ◽  
...  

Abstract. This study presents annual simulations of tropospheric ozone and related species made for the first time using the WRF-Chem model over South Asia for the year 2008. The model simulated ozone, CO, and NOx are evaluated against ground-based, balloon-borne and satellite-borne (TES, OMI and MOPITT) observations. The comparison of model results with surface ozone observations from seven sites and CO and NOx observations from three sites, indicate the model's ability in reproducing seasonal variations of ozone and CO, but show some differences in NOx. The modeled vertical ozone distribution agrees well with the ozone soundings data from two Indian sites. The vertical distributions of TES ozone and MOPITT CO are generally well reproduced, but the model underestimates TES ozone, OMI tropospheric column NO2 and MOPITT total column CO retrievals during all the months except MOPITT retrievals during August–January. Largest differences between modeled and satellite retrieved quantities are found during spring when intense biomass burning activity occurs in this region. The evaluation results indicate large uncertainties in anthropogenic and biomass burning emission estimates, especially for NOx. The model results indicate clear regional differences in the seasonality of surface ozone over South Asia with estimated net ozone production during daytime (11:30–15:30 h) over inland regions of 0–5 ppbv h−1 during all seasons and of 0–2 ppbv h−1 over marine regions during outflow periods. The model results indicate that ozone production in this region is mostly NOx-limited. This study shows that WRF-Chem model captures many important features of the observations and gives confidence to using the model for understanding the spatio-temporal variability of ozone over South Asia. However, improvements of South Asian emission inventories and simulations at finer model resolution, especially over the complex Himalayan terrain in Northern India, are also essential for accurately simulating ozone in this region.


Sign in / Sign up

Export Citation Format

Share Document