Strategy to Establish Embryo-Derived Pluripotent Stem Cells in Cattle

Stem cell research is essential not only for the research and treatment of human diseases, but also for the genetic preservation and improvement of animals. Since embryonic stem cells (ESCs) were established in mice, substantial efforts have been made to establish true ESCs in many species. Although various culture conditions were used to establish ESCs in cattle, the capturing of true bovine ESCs (bESCs) has not been achieved. In this review, the difficulty of establishing bESCs with various culture conditions is described, and the characteristics of proprietary induced pluripotent stem cells and extended pluripotent stem cells are introduced. We conclude with a suggestion of a strategy for establishing true bESCs.

Download Full-text

Induced Pluripotent Stem Cells (iPSCs) in Vascular Research: from Two- to Three-Dimensional Organoids

Stem Cell Reviews and Reports ◽

10.1007/s12015-021-10149-3 ◽

2021 ◽

Author(s):

Anja Trillhaase ◽

Marlon Maertens ◽

Zouhair Aherrahrou ◽

Jeanette Erdmann

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Induced Pluripotent Stem Cells ◽

Stem Cell Research ◽

Pluripotent Stem Cells ◽

Embryonic Stem ◽

Cell Types ◽

3D Models ◽

Induced Pluripotent

AbstractStem cell technology has been around for almost 30 years and in that time has grown into an enormous field. The stem cell technique progressed from the first successful isolation of mammalian embryonic stem cells (ESCs) in the 1990s, to the production of human induced-pluripotent stem cells (iPSCs) in the early 2000s, to finally culminate in the differentiation of pluripotent cells into highly specialized cell types, such as neurons, endothelial cells (ECs), cardiomyocytes, fibroblasts, and lung and intestinal cells, in the last decades. In recent times, we have attained a new height in stem cell research whereby we can produce 3D organoids derived from stem cells that more accurately mimic the in vivo environment. This review summarizes the development of stem cell research in the context of vascular research ranging from differentiation techniques of ECs and smooth muscle cells (SMCs) to the generation of vascularized 3D organoids. Furthermore, the different techniques are critically reviewed, and future applications of current 3D models are reported. Graphical abstract

Download Full-text

Stem Cell Reviews and Reports: Induced Pluripotent Stem Cells, Embryonic Stem Cells and Development Section

Stem Cell Reviews and Reports ◽

10.1007/s12015-017-9722-8 ◽

2017 ◽

Vol 13 (1) ◽

pp. 3-3 ◽

Cited By ~ 3

Author(s):

Henning Ulrich

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Embryonic Stem Cells ◽

Induced Pluripotent Stem Cells ◽

Pluripotent Stem Cells ◽

Embryonic Stem ◽

Development Section ◽

Induced Pluripotent

Download Full-text

Contribution of Mouse Embryonic Stem Cells and Induced Pluripotent Stem Cells to Chimeras through Injection and Coculture of Embryos

Stem Cells International ◽

10.1155/2014/409021 ◽

2014 ◽

Vol 2014 ◽

pp. 1-9 ◽

Cited By ~ 4

Author(s):

Jitong Guo ◽

Baojiang Wu ◽

Shuyu Li ◽

Siqin Bao ◽

Lixia Zhao ◽

...

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Embryonic Stem Cells ◽

Embryonic Stem Cell ◽

Induced Pluripotent Stem Cells ◽

Pluripotent Stem Cells ◽

Embryonic Stem ◽

Germline Transmission ◽

Stem Cell Pluripotency ◽

Induced Pluripotent

Blastocyst injection and morula aggregation are commonly used to evaluate stem cell pluripotency based on chimeric contribution of the stem cells. To assess the protocols for generating chimeras from stem cells, 8-cell mouse embryos were either injected or cocultured with mouse embryonic stem cells and induced pluripotent stem cells, respectively. Although a significantly higher chimera rate resulted from blastocyst injection, the highest germline contribution resulted from injection of 8-cell embryos with embryonic stem cells. The fully agouti colored chimeras were generated from both injection and coculture of 8-cell embryos with embryonic stem cells. Additionally, microsatellite DNA screening showed that the fully agouti colored chimeras were fully embryonic stem cell derived mice. Unlike embryonic stem cells, the mouse chimeras were only generated from injection of 8-cell embryos with induced pluripotent stem cells and none of these showed germline transmission. The results indicated that injection of 8-cell embryos is the most efficient method for assessing stem cell pluripotency and generating induced pluripotent stem cell chimeras, embryonic stem cell chimeras with germline transmission, and fully mouse embryonic stem cell derived mice.

Download Full-text

Perkembangan Terapi Sel Punca (Stem Cell) Pada Penyakit Jantung: Masa Kini dan Masa Depan

Medica Hospitalia : Journal of Clinical Medicine ◽

10.36408/mhjcm.v1i2.47 ◽

2013 ◽

Vol 1 (2) ◽

Author(s):

Idrus Alwi

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Embryonic Stem Cells ◽

Induced Pluripotent Stem Cells ◽

Pluripotent Stem Cells ◽

Embryonic Stem ◽

Cardiac Stem Cells ◽

Induced Pluripotent

Penelitian terapi sel punca di dunia telah berkembang dengan pesat dalam beberapa dasa warsa terakhir, khususnya pada penyakit jantung seperti infark miokard akut, penyakit jantung koroner tahap akhir (PJTA) dan kardiomiopati. Di masa mendatang terapi sel punca dianggap sebagai batas akhir (final frontier) pengobatan berbagai penyakit. Oleh karena itu pengembangan terapi sel punca diharapkan dapat menjawab berbagai tantangan dalam tatalaksana berbagai penyakit secara mendasar. Indonesia merupakan salah satu pelopor terapi sel punca pada infark miokard akut di Asia Tenggara dan merupakan salah satu negara pertama di Asia selain Hongkong yang menggunakan alat NOGA untuk pemetaan (mapping) dan injeksi sel punca pada otot jantung melalui kateterisasi pada PJTA. Sel punca termasuk embryonic stem cells (ESC), cardiac stem cells (CSCs) dan induced pluripotent stem cells (iPSs) dapat mengalami diferensiasi menjadi kardiomiosit dan dapat mengembalikan fungsi kontraksi jantung. Sel tersebut juga dapat berdiferensiasi menjadi sel endotel, memicu pembentukan pembuluh darah baru (angiogenesis), mengembalikan sebagian sel otot jantung yang rusak menjadi tetap hidup, dan menghambat perluasan jaringan parut. Mekanisme lain terapi sel punca pada jantung yang saat ini banyak dianut adalah melalui efek parakrin. Terdapat beberapa metoda pemberian sel punca pada jantung yaitu intrakoroner, epikardial dan intravena. Penelitian di bidang kedokteran kardiovaskular regeneratif mengenai terapi sel punca akan terus berkembang di masa depan, termasuk mengenai jenis sel dan teknik terbaik pemberian sel punca.

Download Full-text