scholarly journals Fibronectin Molecular Status in Plasma of Women with Endometriosis and Fertility Disorders

2021 ◽  
Vol 22 (21) ◽  
pp. 11410
Author(s):  
Jolanta Lis-Kuberka ◽  
Paulina Kubik ◽  
Agnieszka Chrobak ◽  
Jarosław Pająk ◽  
Anna Chełmońska-Soyta ◽  
...  
Keyword(s):  
Wound Healing ◽  
Blood Plasma ◽  
Molecular Mass ◽  
High Molecular Mass ◽  
Normal Group ◽  
Healthy Group ◽  
Healthy Women ◽  
Fertility Disorders ◽  
Chronic Activation ◽  
Coagulation And Fibrinolysis

The diagnosis of endometriosis and fertility disorders is difficult; therefore, it is necessary to look for reliable biomarkers. Analysis of the molecular status of fibronectin as a key player in repair and wound healing processes, as well as in coagulation and fibrinolysis pathways, is justified. ELISA and SDS-agarose immunoblotting were applied to determine the fibronectin concentration and presence and occurrence of soluble FN-fibrin complexes in the blood plasma of women with endometriosis (n = 38), fertility disorders (n = 28) and the healthy group (n = 25). The concentration of fibronectin in the blood plasma of women with endometriosis (292.61 ± 96.17 mg/L) and fertility disorders (287.53 ± 122.68 mg/L) was significantly higher than in the normal group (226.55 ± 91.98 mg/L). The presence of FN-fibrin complexes of 750, 1000, 1300, 1600 and 1900 kDa in the plasma of women with endometriosis and fertility disorders was shown. The presence of FN-fibrin complexes with a molecular mass of more than 1300 kDa in women with endometriosis and infertility and the complete absence of these complexes in healthy women may indicate an increased and chronic activation of coagulation mechanisms in these patients. The presence of complexes of high molecular mass may be one of the biomarkers of fertility disorders in women.

POST-OPERATIVE CHANGES IN FACTOR XIII and FIBRCNETITN

1987 ◽  
Author(s):  
M Reintz ◽  
H Sauer ◽  
G Witzke ◽  
M Neher
Keyword(s):  
Wound Healing ◽  
Blood Plasma ◽  
Factor Xiii ◽  
Rapid Test ◽  
Normal Group ◽  
Marked Rise ◽  
Normal Wound Healing ◽  
Surgery Group ◽  
Group A ◽  
Group B

Wound healing processes after surgery are not restricted to certain areas tut affect the vhole body; the ooagulaticn systan in particular is involved. We studied Factor XIII and fibrcnectin in the blood plasma of 16 patients before surgery, after surgery and cn the 1st, 3rd and 7th days after surgery; fibrcnectin was determined using IC-Partigen iimunodiffusicn and Factor XIII by Behringwerke rapid test. In 11 patients normal wound healing was observed (group A), while in 5 patients explications oocured (group B). Factor XIII: normal group (A): fall in concentration oenpared with the precpera-tive value up to the 7th day after surgery; group with explications (B): fall in concentration more pronounced than in the normal group up o the 7th day after surgery.Fibrcnectin : Normal group (A): Fall in concentration ocpared with the precperative value up to the 1st day after surgery, followed by a rise in concentration; group with ocplicaticns (8): fall in concentration more marked than in the normal group, less marked rise in concentration than in the normal group. Duplications in wound healing were characterized by an increased fall in Factor XIII and fibrcnectin.

High molecular mass kininogen inhibits cathepsin G-induced platelet activation by forming a complex with cathepsin G

2001 ◽  
Vol 2 (6) ◽  
pp. 371-377 ◽  
Author(s):  
Tarek E Selim ◽  
Hayam R Ghoneim ◽  
Hassan A Abdel Ghaffar ◽  
Robert W Colman ◽  
Raul A Dela Cadena
Keyword(s):  
Molecular Mass ◽  
Platelet Activation ◽  
High Molecular Mass ◽  
Cathepsin G

scholarly journals FRI0650-HPR SEXUAL DYSFUNCTION IN WOMEN WITH SYSTEMIC LUPUS ERYTHEMATOSUS

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 929.2-929
Author(s):  
G. V. Espasa ◽  
L. Gonzalez Lucero ◽  
Y. Soria Curi ◽  
A. L. Barbaglia ◽  
S. M. Mazza ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Sexual Dysfunction ◽  
Sexual Function ◽  
Lupus Erythematosus ◽  
Severe Depression ◽  
Systemic Lupus ◽  
Depression Anxiety Stress Scale ◽  
Cross Sectional ◽  
Healthy Group ◽  
Healthy Women

Background:Sexual dysfunction is the alteration in one or several phases of sexual activity (desire, excitement, plateau, orgasm and resolution), which can culminate in frustration, pain and a decrease in the frequency of sexual intercourse. There are few studies that associate sexual dysfunction with Systemic Lupus Erythematosus (SLE) due to the difficulty in assessing it and its multifactorial cause.Objectives:Determine the frequency of sexual dysfunction and analyze associated factors in patients with SLE.Methods:A descriptive cross-sectional study was conducted. We included patients who attended the Rheumatology unit between May and July 2019; over 18 years of age, with a diagnosis of SLE according to the ACR 1997 and / or SLICC 2012 criteria, and healthy patients matched by age as control. Demographic and disease-related variables were studied. The DASS-21 (Depression Anxiety Stress Scale) scale that evaluates depression, anxiety and stress, and the Female Sexual Function Index (FSFI) that assesses 6 domains (desire, excitement, lubrication, orgasms, satisfaction and pain) were applied with a cut-off point ≤ 26.5 to define sexual dysfunction. Women over 50 years old, with secondary Sjogren’s syndrome, menopause, severe depression and illiterate patients were excluded.Results:One hundred and twenty three women were included (60 with SLE and 63 controls), with a mean age of 34.3 ± 8.3 and 31.7 ± 4.4 years respectively. The prevalence of sexual dysfunction in the SLE group was 71.7%; 95% CI = [58.5 – 82.5], and 23.8%, 95% CI = [13.9 – 36.2] in healthy patients. There were significant differences in all domains of sexual function between women with SLE and healthy group. In the desire, excitement and pain domains the differences were notable. The total FSFI score in patients with SLE was 18.2 ± 11.2 and in healthy women 28.3 ± 6.9 (p=0.001). Stress, anxiety and depression were observed in 58.4%, 58.3% and 50% of women with SLE and 19%, 20.6% and 28.5% of healthy women respectively (p=0.001). No association was found between sexual dysfunction and age, age at diagnosis, disease activity or treatment (pNS). No association was found in patients with SLE when analyzing the effect of sexual dysfunction in stress, depression and anxiety variables, in opposition to the healthy group (p<0.05).Conclusion:The prevalence of sexual dysfunction in patients with SLE was high (71.7%). Depression, Anxiety, and Stress were not decisive variables in Sexual Dysfunction.Disclosure of Interests:None declared

scholarly journals Analysis of High Molecular Mass Compounds from the Spider Pamphobeteus verdolaga Venom Gland. A Transcriptomic and MS ID Approach

Toxins ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 453
Author(s):  
Sebastian Estrada-Gómez ◽  
Leidy Johana Vargas-Muñoz ◽  
Cesar Segura Latorre ◽  
Monica Maria Saldarriaga-Cordoba ◽  
Claudia Marcela Arenas-Gómez
Keyword(s):  
Enzymatic Activity ◽  
Molecular Mass ◽  
Evolutionary Relationship ◽  
Venom Gland ◽  
Duplication Event ◽  
High Molecular Mass ◽  
Amino Acid Sequences ◽  
Secretory Proteins ◽  
Phospholipases A2 ◽  
Kunitz Type

Nowadays, spider venom research focuses on the neurotoxic activity of small peptides. In this study, we investigated high-molecular-mass compounds that have either enzymatic activity or housekeeping functions present in either the venom gland or venom of Pamphobeteus verdolaga. We used proteomic and transcriptomic-assisted approaches to recognize the proteins sequences related to high-molecular-mass compounds present in either venom gland or venom. We report the amino acid sequences (partial or complete) of 45 high-molecular-mass compounds detected by transcriptomics showing similarity to other proteins with either enzymatic activity (i.e., phospholipases A2, kunitz-type, hyaluronidases, and sphingomyelinase D) or housekeeping functions involved in the signaling process, glucanotransferase function, and beta-N-acetylglucosaminidase activity. MS/MS analysis showed fragments exhibiting a resemblance similarity with different sequences detected by transcriptomics corresponding to sphingomyelinase D, hyaluronidase, lycotoxins, cysteine-rich secretory proteins, and kunitz-type serine protease inhibitors, among others. Additionally, we report a probably new protein sequence corresponding to the lycotoxin family detected by transcriptomics. The phylogeny analysis suggested that P. verdolaga includes a basal protein that underwent a duplication event that gave origin to the lycotoxin proteins reported for Lycosa sp. This approach allows proposing an evolutionary relationship of high-molecular-mass proteins among P. verdolaga and other spider species.

scholarly journals Identification of two highly sialylated human tear-fluid DMBT1 isoforms: the major high-molecular-mass glycoproteins in human tears

2002 ◽  
Vol 366 (2) ◽  
pp. 511-520 ◽  
Author(s):  
Benjamin L. SCHULZ ◽  
David OXLEY ◽  
Nicolle H. PACKER ◽  
Niclas G. KARLSSON
Keyword(s):  
Molecular Mass ◽  
Peptide Mass Fingerprinting ◽  
High Molecular Mass ◽  
Tear Fluid ◽  
Protein Variant ◽  
Peptide Mass ◽  
Composite Gel ◽  
Molecular Masses ◽  
Protein Components ◽  
Sialyl Lea

Human open eye tear fluid was separated by low-percentage SDS/PAGE to detect high-molecular-mass protein components. Two bands were found with apparent molecular masses of 330 and 270kDa respectively. By peptide-mass fingerprinting after tryptic digestion, the proteins were found to be isoforms of the DMBT1 gene product, with over 30% of the predicted protein covered by the tryptic peptides. By using gradient SDS/agarose/polyacrylamide composite gel electrophoresis and staining for glycosylation, it was shown that the two isoforms were the major high-molecular-mass glycoproteins of >200kDa in human tear fluid. Western blotting showed that the proteins expressed sialyl-Lea. After the release of oligosaccharides by reductive β-elimination from protein blotted on to PVDF membrane, it was revealed by liquid chromatography-MS that the O-linked oligosaccharides were comprised mainly of highly sialylated oligosaccharides with up to 16 monosaccharide units. A majority of the oligosaccharides could be described by the formula dHex0→2NeuAc1→xHexxHexNAcx(-ol), x = 1–6, where Hex stands for hexose, dHex for deoxyhexose, HexNAc for N-acetylhexosamine and NeuAc for N-acetylneuraminate. The number of sialic acids in the formula is less than 5. Interpretation of collision-induced fragmentation tandem MS confirmed the presence of sialic acid and suggested the presence of previously undescribed structures carrying the sialyl-Lea epitopes. Small amounts of neutral and sulphated species were also present. This is the first time that O-linked oligosaccharides have been detected and described from protein variant of the DMBT1 gene.

High-Molecular-Mass Receptors for Ammodytoxin in Pig Are Tissue-Specific Isoforms of M-Type Phospholipase A2 Receptor

2001 ◽  
Vol 289 (1) ◽  
pp. 143-149 ◽  
Author(s):  
Nina Vardjan ◽  
Nicholas E Sherman ◽  
Jože Pungerčar ◽  
Jay W Fox ◽  
Franc Gubenšek ◽  
...  
Keyword(s):  
Phospholipase A2 ◽  
Molecular Mass ◽  
High Molecular Mass ◽  
Tissue Specific ◽  
Phospholipase A2 Receptor
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