scholarly journals Collagenated Synthetic Bone Substitute Material for Sinus Floor Elevation at Sites with a Perforated Schneiderian Membrane

2020 ◽  
Vol 9 (11) ◽  
pp. 3764
Author(s):  
Sangyup Kim ◽  
Jong-Hyuk Chung ◽  
Seung-Yun Shin ◽  
Seung-Il Shin ◽  
Ji-Youn Hong ◽  
...  
Keyword(s):  
Bone Formation ◽  
Dimensional Stability ◽  
Bone Substitute ◽  
Control Group ◽  
New Bone Formation ◽  
Bone Substitute Material ◽  
Sinus Floor Elevation ◽  
Schneiderian Membrane ◽  
Sinus Floor ◽  
Substitute Material

Schneiderian membrane perforation (SMP) is the most common complication during sinus floor elevation (SFE). Conventional methods to repair SMP, such as using a collagen barrier, may be clinically demanding. The aim of the present study was to compare the effects of collagenated bone substitute materials with and without a collagen barrier to repair SMP during SFE in terms of new bone formation and dimensional stability. In 12 rabbits, intentional SMP was made during bilateral SFE. The rabbits were randomly assigned under two groups: the control group, in which the sinus was repaired with a collagen barrier, and the test group, in which the sinus was repaired without a collagen barrier. Collagenated bone substitute material was grafted in both groups. Healing periods of 2 weeks and 4 weeks were provided in both groups. There were no adverse clinical events. Histology revealed that the Schneiderian membrane had atrophied with loss of cilia and serous glands in both groups at 4 weeks. Histomorphometry revealed that the newly formed bone (test: 0.42 ± 0.17 mm2, control: 0.36 ± 0.18 mm2 at 2 weeks; test: 1.21 ± 0.36 mm2, control: 1.23 ± 0.55 mm2 at 4 weeks) or total augmented area did not significantly differ between the two groups at either time points (p > 0.05). In conclusion, collagenated bone substitute material without a collagen barrier demonstrated similar new bone formation and dimensional stability as that with a collagen barrier in repairing SMP.

scholarly journals The use of solvent-preserved human and bovine cancellous bone blocks for lateral defect augmentation - an experimental controlled study in vivo

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Lara Schorn ◽  
Tim Fienitz ◽  
Kathrin Berndsen ◽  
Norbert R. Kübler ◽  
Henrik Holtmann ◽  
...  
Keyword(s):  
Bone Formation ◽  
Cancellous Bone ◽  
Bone Substitute ◽  
Bone Matrix ◽  
Human Bone ◽  
Controlled Study ◽  
Bovine Bone ◽  
New Bone Formation ◽  
Bone Substitute Material ◽  
Substitute Material

Abstract Background The aim of this study was to compare new bone formation, resorbed bone matrix, and fibrous enclosed residual bone substitute material in laterally augmented alveolar bone defects using allogeneic, pre-treated and cleaned human bone blocks (tested in dogs, therefore considered to be xenogeneic), and pre-treated and cleaned bovine cancellous bone blocks, both with and without a collagen membrane in order to evaluate their augmentative potential. Methods Thirty-two critical size horizontal defects were prepared in the mandible of 4 adult foxhound dogs (8 per dog, 4 on each side). After 3 months of healing, the defects were laterally augmented in a split-mouth-design with either human (HXB) or bovine solvent-preserved bone blocks (BXB). Afterwards, defects were randomly covered with a bovine collagenous membrane (HXB + M, BXB + M). After a healing interval of 6 months, percentages of new bone formation, resorbed bone matrix, and fibrous enclosed residual bone substitute material were compared. Results Results showed little new bone formation of up to 3.7 % in human bone blocks (HXB 3.7 % ± 10.2, HXB + M 0.3 %± 0.4, BXB, 0.1 % ± 0.8, BXB + M 2.6 % ± 3.2, p = > 0.05). Percentages of fibrous encapsulation were higher in human bone blocks than in bovine bone blocks (HXB 71.2 % ± 8.6, HXB + M 73.71 % ± 10.6, BXB, 60.5 % ± 27.4, BXB + M 52.5 % ± 28.4, p = > 0.05). Resorption rates differed from 44.8 % in bovine bone blocks covered with a membrane to 17.4 % in human bone blocks (HXB 17.4 % ± 7.4, HXB + M 25.9 % ± 10.7, BXB, 38.4 % ± 27.2, BXB + M 44.8 % ± 29.6, p = > 0.05). The use of additional membranes did not significantly affect results. Conclusions Within its limitations, results of this study suggest that solvent-preserved xenogenic human and bovine bone blocks are not suitable for lateral bone augmentation in dogs. Furthermore, defect coverage with a membrane does not positively affect the outcome.

scholarly journals Bone Augmentation of Peri-Implant Dehiscence Defects Using Multilaminated Small Intestinal Submucosa as a Barrier Membrane: An Experimental Study in Dogs

2019 ◽  
Vol 2019 ◽  
pp. 1-11
Author(s):  
Siwen Wang ◽  
Weiyi Wu ◽  
Yuhua Liu ◽  
Xinzhi Wang ◽  
Lin Tang ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Substitute ◽  
Small Intestinal Submucosa ◽  
Control Group ◽  
Collagen Membrane ◽  
Micro Ct ◽  
Bone Substitute Material ◽  
Substitute Material ◽  
Small Intestinal ◽  
Intestinal Submucosa

Objective. The aim of the study is to evaluate the effects of multilaminated small intestinal submucosa (mSIS) combined with bone substitute material to repair peri-implant defects during guided bone regeneration procedures. Methods. Twelve implants were placed in bilateral lower premolars of three beagle dogs, and a peri-implant buccal bone defect (3 mm width and 4 mm height) was created at each implant site. A total of 12 sites were filled with a particulate bone substitute material and then randomly divided into three treatment groups: covered by mSIS membrane (mSIS group), covered by collagen membrane (BG group), and no treatment (control group), each group of four sites. After 12 weeks of healing, all of the animals were euthanized and dissected blocks were obtained for micro-computed tomography (micro-CT) and histological analyses. Results. Micro-CT results revealed similar horizontal width of augmented tissue and new bone formation between mSIS and BG groups (P<0.05). Histological analyses revealed that the differences in horizontal widths of newly formed bone and bone-to-implant contact between mSIS and BG groups were not significant (P>0.05). All of these parameters were significantly different from those in the control group (P<0.05). Conclusions. These findings confirmed that mSIS combined with the bone substitute material enhanced bone regeneration in peri-implant defects, in a manner similar to that of a collagen membrane.

Influence of Maxillary Sinus Width on New Bone Formation After Transcrestal Sinus Floor Elevation

2017 ◽  
Vol 26 (2) ◽  
pp. 209-216 ◽  
Author(s):  
Teresa Lombardi ◽  
Claudio Stacchi ◽  
Federico Berton ◽  
Tonino Traini ◽  
Lucio Torelli ◽  
...  
Keyword(s):  
Bone Formation ◽  
Maxillary Sinus ◽  
New Bone Formation ◽  
Sinus Floor Elevation ◽  
Sinus Floor

Bone Regenerative Properties of Injectable Calcium Phosphate/PLGA Cement in an Alveolar Bone Defect

2012 ◽  
Vol 529-530 ◽  
pp. 300-303 ◽  
Author(s):  
R.P. Félix Lanao ◽  
J.W.M. Hoekstra ◽  
Joop G.C. Wolke ◽  
Sander C.G. Leeuwenburgh ◽  
A.S. Plachokova ◽  
...  
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Calcium Phosphate ◽  
Bone Formation ◽  
Bone Substitute ◽  
Alveolar Bone ◽  
Bone Defects ◽  
Plga Microspheres ◽  
Bone Substitute Material ◽  
Substitute Material

Periodontitis is one of the most common inflammatory diseases, which can lead to early tooth loss. The conventional treatment of periodontitis is to arrest the disease progression. Most reconstructive procedures involve application of bone substitutes, barrier membranes or a combination of both into the bony defects. Calcium phosphate cements (CPCs) are the predominant type of bone substitute material used for reasons of injectability and hence perfect filling potential for bone defects. Recently, injectable apatitic CPCs demonstrated to be more rapidly degradable when combined with poly (lactic-co-glycolic) acid (PLGA) microspheres. Further, PLGA microspheres can be used as a delivery vehicle for growth factors. In this study, the performance of injectable CPCs as a bone substitute material for alveolar bone defects created in Beagle dogs was evaluated. Four CPC-formulations were generated by incorporating hollow or dense PLGA microspheres, either or not loaded with the growth factors (platelet derived growth factor (PDGF) and insulin-like growth factor (IGF). Implantation period was 8 weeks. Bone formation was based on histological and histomorphometrical evaluation. The results demonstrated that filling alveolar bone defects with CPC-dense PLGA revealed significant more bone formation compared to CPC-hollow PLGA either or not loaded with IGF and PDGF. In summary, we conclude that injectable CPC-dense PLGA composites proved to be the most suitable material for a potential use as off the shelf material due to its good biocompatibility, enhanced degradability and subsequent bone formation.

scholarly journals Gene expression, immunohistochemical and microarchitectural evaluation of bone formation around two implant surfaces placed in bone defects filled or not with bone substitute material

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Guilherme dos Santos Trento ◽  
Jaqueline Suemi Hassumi ◽  
Paula Buzo Frigério ◽  
Ana Paula Farnezi Bassi ◽  
Roberta Okamoto ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Defect ◽  
Bone Substitute ◽  
Blood Clot ◽  
Titanium Implant ◽  
Hydrophilic Surface ◽  
Micro Ct ◽  
Bone Substitute Material ◽  
Ct Analysis ◽  
Substitute Material

Abstract Objective The aim of this study is to evaluate through gene expression, immunohistochemical and microtomographic (micro-CT) analysis the response of peri-implant bone tissue around titanium implants with different surface treatments, placed in bone defects filled or not with bone substitute materials. In addition, to investigate the hypothesis that porous-hydrophilic surface induces a faster bone formation. Materials and methods Twenty-six animals were divided into two groups according to implant surface treatment. In each tibia, a bone defect was created followed by the placement of one implant. On the left tibia, the defect was filled with blood clot (BC), and on the right tibia, the defect was filled with biphasic hydroxyapatite/β-tricalcium-phosphate (HA/TCP) generating four subgroups: BC-N: bone defect filled with blood clot and porous surface titanium implant installed; BC-A: bone defect filled with blood clot and porous-hydrophilic surface titanium implant installed; HA/TCP-N: bone defect filled with bone substitute material and porous surface titanium implant installed; and HA/TCP-A: bone defect filled with bone substitute material and porous-hydrophilic surface titanium implant installed. The animals were submitted to euthanasia at 15, 30, and 60 days after implant installation. The expression of two genes was evaluated: RUNX2 and BSP. Immunohistochemical analyses were performed for detection of RUNX2, OPN, OCN, OPG, and RANKL antibodies and bone matrix proteins. Finally, four parameters were chosen for micro-CT analysis: trabecular number, separation and thickness, and connectivity density. Results Descriptive analysis showed similar findings among the experimental groups. Moreover, porous-hydrophilic surfaces presented a higher expression of RUNX2, which is probably an indicative of better osteogenesis; although the data from this study may be considered an insufficient support for a concrete statement. Conclusion Porous hydrophilic surface can improve and accelerate protein expression and bone formation.

New bone formation after transcrestal sinus floor elevation was influenced by sinus cavity dimensions: A prospective histologic and histomorphometric study

2018 ◽  
Vol 29 (5) ◽  
pp. 465-479 ◽  
Author(s):  
Claudio Stacchi ◽  
Teresa Lombardi ◽  
Roberto Ottonelli ◽  
Federico Berton ◽  
Giuseppe Perinetti ◽  
...  
Keyword(s):  
Bone Formation ◽  
New Bone Formation ◽  
Sinus Floor Elevation ◽  
Histomorphometric Study ◽  
Sinus Cavity ◽  
Sinus Floor

Combined study on the action and mechanism of G-Rg1/Sr-CaS bone substitute material for ossification and pro-vascularization

2020 ◽  
Vol 10 (2) ◽  
pp. 177-189
Author(s):  
Xue Li ◽  
Shi Hong ◽  
Caixia Tan ◽  
Bo Peng ◽  
Zhengjie Wu ◽  
...  
Keyword(s):  
Bone Substitute ◽  
Bone Repair ◽  
Umbilical Vein ◽  
Research Work ◽  
Calcium Sulphate ◽  
Control Group ◽  
Mouse Bone Marrow ◽  
Bone Substitute Material ◽  
Substitute Material

Neovascularization is important for bone repair, vascularization, and ossification during bone repair. Ginsenoside Rg1 (G-Rg1), which is the main extract of ginseng, has been shown to promote therapeutic angiogenesis. It has been studied in the field of biomaterials, but there is no relevant report in the field of bone substitute materials. In this study, we successfully prepared the bone substitute material combining calcium sulphate (Sr-CaS) with G-Rg1 on the basis of previous research work. In vitro experiments were carried out to verify the ossification of composites by using mouse bone marrow mesenchymal stem cells (BMMSCs) and the ossification was quantified by western blot. The related proteins in the key signaling pathways for the different concentrations of G-Rg1/Sr-CaS composite extract were studied to determine whether there was receptor competition and to find the optimal ratio parameters. The vascularization of the composite was verified in the human umbilical vein endothelial cells (HUVECs) model, and finally the coordination of pro-vascularization and ossification was evaluated in the mouse critical bone defect model. The results indicated that G-Rg1/Sr-CaS composites contributed to ossification in the mouse BMMSC model and vascularization in the HUVEC model. The G-Rg1/Sr-CaS composites resulted in significantly greater bone mineral densities and bone volume/total volume of the defect group compared to the control group. Histological analysis showed that the G-Rg1/SrCaS was resorbable with satisfactory biocompatibility. The doped strontium ions enhanced the bone repair performance of G-Rg1/Sr-CaS in the mouse model and the new substitute demonstrated promising results for clinical use.

Effect of water glass coating of tricalcium phosphate granules on in vivo bone formation

2018 ◽  
Vol 33 (5) ◽  
pp. 662-672
Author(s):  
Seung Min Ryu ◽  
Myun Whan Ahn ◽  
Chul Hyun Park ◽  
Gun Woo Lee ◽  
In Hwan Song ◽  
...  
Keyword(s):  
Bone Formation ◽  
Tricalcium Phosphate ◽  
Bone Substitute ◽  
Water Glass ◽  
Cylindrical Tube ◽  
Control Group ◽  
New Bone Formation ◽  
Group A ◽  
Group B

Background Recently, some authors introduced a water glass (WG, sodium-silicate glass; Na2O·SiO2·nH2O) coating over tricalcium phosphate (TCP) bioceramic to modulate its resorption rate and enhance the bone cell behaviors. In this study, four different types of granular samples were prepared to evaluate the ability of new bone formation in vivo using micro-computed tomography and histology. Methods Four types sample groups: group A (pure HA as a negative resorption control); group B (pure TCP as a positive resorption control); group C (WG-coated TCP as an early resorption model); and group D (same as group C but heat-treated at 500°C as a delayed resorption model). Cylindrical tube-type carriers with holes were fabricated with HA by extrusion and sintering. Each carrier was filled densely with each granular sample. Four types of tubes were implanted into the medial femoral condyle and medial tibial condyle of New Zealand White rabbits. Results The HA group (A) showed the lowest amount of new bone formation. All the TCP sample groups (B, C, and D) showed more new bone formation. On the other hand, among the TCP groups, group C (early resorption model) showed slightly more bone formation. The amount of residual bioceramics was most abundant in the HA group (A). All the TCP sample groups showed less residual bioceramics than group A. Among the TCP groups, group C showed slightly more residual bioceramics. Group B showed the lowest amount of residual bioceramics. Conclusions The WG-coated TCP sample (group C) is the best bone substitute candidate because of its proper biodegradation rate and the Si ions release because the WG-coated layer reduces the material resorption and enhances the new bone formation. That is, the WG-coated TCP is believed to be the best material for the application of an artificial bone substitute material.

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