scholarly journals Formulation and Characterization of a New Injectable Bone Substitute Composed PVA/Borax/CaCO3 and Demineralized Bone Matrix

2021 ◽  
Vol 12 (3) ◽  
pp. 46
Author(s):  
Daniela Medrano-David ◽  
Aura María Lopera ◽  
Martha Elena Londoño ◽  
Pedronel Araque-Marín
Keyword(s):  
Bone Substitute ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Particulate Material ◽  
Bone Tissue Regeneration ◽  
Bone Implant ◽  
Injectable Scaffold ◽  
The World ◽  
Demineralized Bone

The occurrence of bone-related disorders and diseases has dramatically increased in recent years around the world. Demineralized bone matrix (DBM) has been widely used as a bone implant due to its osteoinduction and bioactivity. However, the use of DBM is limited because it is a particulate material, which makes it difficult to manipulate and implant with precision. In addition, these particles are susceptible to migration to other sites. To address this situation, DBM is commonly incorporated into a variety of carriers. An injectable scaffold has advantages over bone grafts or preformed scaffolds, such as the ability to flow and fill a bone defect. The aim of this research was to develop a DBM carrier with such viscoelastic properties in order to obtain an injectable bone substitute (IBS). The developed DBM carrier consisted of a PVA/glycerol network cross-linked with borax and reinforced with CaCO3 as a pH neutralizer, porosity generator, and source of Ca. The physicochemical properties were determined by an injectability test, FTIR, SEM, and TGA. Porosity, degradation, bioactivity, possible cytotoxic effect, and proliferation in osteoblasts were also determined. The results showed that the developed material has great potential to be used in bone tissue regeneration.

scholarly journals Formulation and Characterization of a New Injectable Bone Substitute Composed PVA/borax /CaCO3 and Demineralized Bone Matrix

2021 ◽  
Author(s):  
Daniela Medrano-David ◽  
Aura Maria Lopera ◽  
Martha Elena Londoño ◽  
Pedronel Araque-Marín
Keyword(s):  
Bone Substitute ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Particulate Material ◽  
Bone Tissue Regeneration ◽  
Bone Implant ◽  
Injectable Scaffold ◽  
The World ◽  
Demineralized Bone

The occurrence of bone-related disorders and diseases has increased dramatically in recent years around the world. Demineralized bone matrix (DBM) has been widely used as a bone implant due to its osteoinduction and bioactivity. However, the use of DBM is limited because it is a particulate material, which makes it difficult to manipulate and implant with precision, in addition, these particles are susceptible to migrate to other sites. To address this situation, DBM is commonly incorporated into a variety of carriers. An injectable scaffold has advantages over bone grafts or preformed scaffolds, such as the ability to flow and fill the bone defect. The aim of this research is to develop a DBM carrier with such viscoelastic properties to obtain an injectable bone substitute (IBS). The DBM carrier developed consisted of a PVA/glycerol network cross-linked with borax and reinforced with CaCO3 as a pH neutralizer, porosity generator, and source of Ca. The physicochemical properties were determined by the injectability test, FTIR, SEM, and TGA. Porosity, degradation, bioactivity, possible cytotoxic effect, and proliferation in osteoblasts were also determined. The results show that the developed material has great potential to be used in bone tissue regeneration

Characterization of Bone Defect Repair in Young and Aged Rat Femur Induced by Xenogenic Demineralized Bone Matrix

2002 ◽  
Vol 73 (9) ◽  
pp. 1003-1009 ◽  
Author(s):  
Paola Torricelli ◽  
Milena Fini ◽  
Gianluca Giavaresi ◽  
Lia Rimondini ◽  
Roberto Giardino
Keyword(s):  
Bone Defect ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Rat Femur ◽  
Aged Rat ◽  
Bone Defect Repair ◽  
Defect Repair ◽  
Demineralized Bone

scholarly journals Implant Composed of Demineralized Bone and Mesenchymal Stem Cells Genetically Modified with AdBMP2/AdBMP7 for the Regeneration of Bone Fractures inOvis aries

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Adelina A. Hernandez-Hurtado ◽  
Gissela Borrego-Soto ◽  
Ivan A. Marino-Martinez ◽  
Jorge Lara-Arias ◽  
Viktor J. Romero-Diaz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Fractures ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Adenoviral Vectors ◽  
Bone Tissue Regeneration ◽  
Demineralized Bone

Adipose-derived mesenchymal stem cells (ADMSCs) are inducible to an osteogenic phenotype by the bone morphogenetic proteins (BMPs). This facilitates the generation of implants for bone tissue regeneration. This study evaluated thein vitroosteogenic differentiation of ADMSCs transduced individually and in combination with adenoviral vectors expressing BMP2 and BMP7. Moreover, the effectiveness of the implant containing ADMSCs transduced with the adenoviral vectors AdBMP2/AdBMP7 and embedded in demineralized bone matrix (DBM) was tested in a model of tibial fracture in sheep. This graft was compared to ewes implanted with untransduced ADMSCs embedded in the same matrix and with injured but untreated animals.In vivoresults showed accelerated osteogenesis in the group treated with the AdBMP2/AdBMP7 transduced ADMSC graft, which also showed improved restoration of the normal bone morphology.

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