scholarly journals What Role Might Non-Mating Receptors Play in Schizophyllum commune?

2021 ◽  
Vol 7 (5) ◽  
pp. 399
Author(s):  
Sophia Wirth ◽  
Daniela Freihorst ◽  
Katrin Krause ◽  
Erika Kothe
Keyword(s):  
Mating Type ◽  
Developmental Stages ◽  
Functional Characterization ◽  
Schizophyllum Commune ◽  
Growth Direction ◽  
Type Locus ◽  
Mating Type Locus ◽  
High Level ◽  
New Evidence

The B mating-type locus of the tetrapolar basidiomycete Schizophyllum commune encodes pheromones and pheromone receptors in multiple allelic specificities. This work adds substantial new evidence into the organization of the B mating-type loci of distantly related S. commune strains showing a high level of synteny in gene order and neighboring genes. Four pheromone receptor-like genes were found in the genome of S. commune with brl1, brl2 and brl3 located at the B mating-type locus, whereas brl4 is located separately. Expression analysis of brl genes in different developmental stages indicates a function in filamentous growth and mating. Based on the extensive sequence analysis and functional characterization of brl-overexpression mutants, a function of Brl1 in mating is proposed, while Brl3, Brl4 and Brl2 (to a lower extent) have a role in vegetative growth, possible determination of growth direction. The brl3 and brl4 overexpression mutants had a dikaryon-like, irregular and feathery phenotype, and they avoided the formation of same-clone colonies on solid medium, which points towards enhanced detection of self-signals. These data are supported by localization of Brl fusion proteins in tips, at septa and in not-yet-fused clamps of a dikaryon, confirming their importance for growth and development in S. commune.

scholarly journals Transcriptome and Functional Analysis of Mating in the Basidiomycete Schizophyllum commune

2011 ◽  
Vol 11 (5) ◽  
pp. 571-589 ◽  
Author(s):  
Susann Erdmann ◽  
Daniela Freihorst ◽  
Marjatta Raudaskoski ◽  
Wolfgang Schmidt-Heck ◽  
Elke-Martina Jung ◽  
...  
Keyword(s):  
Mating Type ◽  
Fluorescent Protein ◽  
Expression Patterns ◽  
Functional Characterization ◽  
Schizophyllum Commune ◽  
Northern Hybridization ◽  
Cell Periphery ◽  
Dependent Manner ◽  
Type Locus ◽  
Mating Type Locus

ABSTRACTIn this study, we undertook a functional characterization and transcriptome analysis that enabled a comprehensive study of the mating type loci of the mushroomSchizophyllum commune. Induced expression of both thebar2receptor and thebap2(2)pheromone gene within 6 to 12 h after mates' contact was demonstrated by quantitative real-time PCR. Similar temporal expression patterns were confirmed for the allelicbbr1receptor andbbp1pheromone-encoding genes by Northern hybridization. Interestingly, the fusion of clamp connections to the subterminal cell was delayed in mating interactions in which one of the compatible partners expressed thebar2receptor with a truncated C terminus. This developmental delay allowed the visualization of a green fluorescent protein (Gfp)-labeled truncated receptor at the cell periphery, consistent with a localization in the plasma membrane of unfused pseudoclamps. This finding does not support hypotheses envisioning a receptor localization to the nuclear membrane facilitating recognition between the two different nuclei present in each dikaryotic cell. Rather, Gfp fluorescence observed in such pseudoclamps indicated a role of receptor-pheromone interaction in clamp fusion. Transcriptome changes associated with mating interactions were analyzed in order to identify a role for pheromone-receptor interactions. We detected a total of 89 genes that were transcriptionally regulated in a mating type locusA-dependent manner, employing a cutoff of 5-fold changes in transcript abundance. Upregulation in cell cycle-related genes and downregulation of genes involved in metabolism were seen with this set of experiments. In contrast, mating type locusB-dependent transcriptome changes were observed in 208 genes, with a specific impact on genes related to cell wall and membrane metabolism, stress response, and the redox status of the cell.

scholarly journals The mating-type locus B alpha 1 of Schizophyllum commune contains a pheromone receptor gene and putative pheromone genes.

1995 ◽  
Vol 14 (21) ◽  
pp. 5271-5278 ◽  
Author(s):  
J. Wendland ◽  
L. J. Vaillancourt ◽  
J. Hegner ◽  
K. B. Lengeler ◽  
K. J. Laddison ◽  
...  
Keyword(s):  
Mating Type ◽  
Receptor Gene ◽  
Schizophyllum Commune ◽  
Type Locus ◽  
Pheromone Receptor ◽  
Mating Type Locus

The Aα mating-type locus of Schizophyllum commune: structure and function of gene X

1996 ◽  
Vol 29 (2) ◽  
pp. 143-149
Author(s):  
A. L. Marion ◽  
Kirk A. Bartholomew ◽  
Jian Wu ◽  
H. Yang ◽  
Charles P. Novotny ◽  
...  
Keyword(s):  
Mating Type ◽  
Schizophyllum Commune ◽  
Structure And Function ◽  
Type Locus ◽  
Mating Type Locus ◽  
And Function

The Aα Mating Type Locus of Schizophyllum commune

1991 ◽  
pp. 234-257 ◽  
Author(s):  
CHARLES P. NOVOTNY ◽  
MARY M. STANKIS ◽  
CHARLES A. SPECHT ◽  
HUILING YANG ◽  
ROBERT C. ULLRICH ◽  
...  
Keyword(s):  
Mating Type ◽  
Schizophyllum Commune ◽  
Type Locus ◽  
Mating Type Locus

scholarly journals rme1 Mutation of Saccharomyces cerevisiae: map position and bypass of mating type locus control of sporulation.

1981 ◽  
Vol 1 (10) ◽  
pp. 958-960 ◽  
Author(s):  
J Rine ◽  
G F Sprague ◽  
I Herskowitz
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mating Type ◽  
Type Locus ◽  
Mating Type Locus ◽  
Type Information

Sporulation in Saccharomyces cerevisiae normally occurs only in MATa/MAT alpha diploids. We show that mutations in RME1 bypassed the requirements for both a and alpha mating type information in sporulation and therefore allowed MATa/MATa and MAT alpha/MAT alpha diploids to sporulate. RME1 was located on chromosome VII, between LEU1 and ADE6.

Transformation of protoplasted yeast cells is directly associated with cell fusion

1984 ◽  
Vol 4 (4) ◽  
pp. 771-778
Author(s):  
S Harashima ◽  
A Takagi ◽  
Y Oshima
Keyword(s):  
Cell Fusion ◽  
Mating Type ◽  
Nuclear Fusion ◽  
Yeast Cells ◽  
Nuclear Markers ◽  
Type Locus ◽  
Mating Type Locus ◽  
Yeast Protoplasts ◽  
Yeast Plasmids ◽  
Binominal Distribution

The frequency of cell fusion during transformation of yeast protoplasts with various yeast plasmids with a chromosome replicon (YRp or YCp) or 2 mu DNA (YEp) was estimated by two methods. In one method, a mixture of protoplasts of two haploid strains with identical mating type and complementary auxotrophic nuclear markers with or without cytoplasmic markers was transformed. When the number of various phenotypic classes of transformants for the nuclear markers was analyzed by equations derived from binominal distribution theory, the frequency of nuclear fusion among the transformants was 42 to 100% in transformations with the YRp or YCp plasmids and 28 to 39% with the YEp plasmids. In another method, a haploid bearing the sir mutation, which allows a diploid (or polyploid) homozygous for the MAT (mating type) locus to sporulate by the expression of the silent mating-type loci HML and HMR, was transformed with the plasmids. Sporulation ability was found in 43 to 95% of the transformants with the YRp or YCp plasmids, and 26 to 31% of the YEp transformants. When cytoplasmic mixing was included with the nuclear fusion, 96 to 100% of the transformants were found to be cell fusants. Based upon these observations, we concluded that transformation of yeast protoplasts is directly associated with cell fusion.

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