scholarly journals The Effects of Dark Incubation on Cellular Metabolism of the Wild Type Cyanobacterium Synechocystis sp. PCC 6803 and a Mutant Lacking the Transcriptional Regulator cyAbrB2

Life ◽  
2014 ◽  
Vol 4 (4) ◽  
pp. 770-787 ◽  
Author(s):  
Masamitsu Hanai ◽  
Yusuke Sato ◽  
Atsuko Miyagi ◽  
Maki Kawai-Yamada ◽  
Kyoko Tanaka ◽  
...  
Keyword(s):  
Cellular Metabolism ◽  
Transcriptional Regulator ◽  
Wild Type ◽  
Dark Incubation ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Deletion of the Transcriptional Regulator cyAbrB2 Deregulates Primary Carbon Metabolism in Synechocystis sp. PCC 6803

2013 ◽  
Vol 162 (2) ◽  
pp. 1153-1163 ◽  
Author(s):  
Yuki Kaniya ◽  
Ayumi Kizawa ◽  
Atsuko Miyagi ◽  
Maki Kawai-Yamada ◽  
Hirofumi Uchimiya ◽  
...  
Keyword(s):  
Carbon Metabolism ◽  
Transcriptional Regulator ◽  
Synechocystis Sp ◽  
Pcc 6803

A novel transcriptional regulator, Sll1130, negatively regulates heat-responsive genes in Synechocystis sp. PCC6803

2013 ◽  
Vol 449 (3) ◽  
pp. 751-760 ◽  
Author(s):  
Pilla Sankara Krishna ◽  
Balaga Radha Rani ◽  
M. Karthik Mohan ◽  
Iwane Suzuki ◽  
Sisinthy Shivaji ◽  
...  
Keyword(s):  
Heat Shock ◽  
Transcriptional Regulator ◽  
Hypothetical Protein ◽  
Upstream Region ◽  
Wild Type ◽  
Protein Levels ◽  
Shock Response ◽  
Inducible Genes ◽  
Hypothetical Genes ◽  
Synechocystis Sp

A conserved hypothetical protein, Sll1130, is a novel transcription factor that regulates the expression of major heat-responsive genes in Synechocystis sp. PCC6803. Synechocystis exhibited an increased thermotolerance due to disruption of sll1130. Δsll1130 cells recovered much faster than wild-type cells after they were subjected to heat shock (50°C) for 30 min followed by recovery at 34°C for 48 h. In Δsll1130 cultures, 70% of the cells were viable compared with the wild-type culture in which only 30% of the cells were viable. DNA microarray analysis revealed that in Δsll1130, expression of the heat-responsive genes such as htpG, hspA, isiA, isiB and several hypothetical genes were up-regulated. Sll1130 binds to a conserved inverted-repeat (GGCGATCGCC) located in the upstream region of the above genes. In addition, both the transcript and protein levels of sll1130 were immediately down-regulated upon shift of wild-type cells from 34 to 42°C. Collectively the results of the present study suggest that Sll1130 is a heat-responsive transcriptional regulator that represses the expression of certain heat-inducible genes at optimum growth temperatures. Upon heat shock, a quick drop in the Sll1130 levels leads to de-repression of the heat-shock genes and subsequent thermal acclimation. On the basis of the findings of the present study, we present a model which describes the heat-shock response involving Sll1130.

scholarly journals The Malic Enzyme Is Required for Optimal Photoautotrophic Growth of Synechocystis sp. Strain PCC 6803 under Continuous Light but Not under a Diurnal Light Regimen

2004 ◽  
Vol 186 (23) ◽  
pp. 8144-8148 ◽  
Author(s):  
Terry M. Bricker ◽  
Shulu Zhang ◽  
Susan M. Laborde ◽  
Paul R. Mayer ◽  
Laurie K. Frankel ◽  
...  
Keyword(s):  
Malic Enzyme ◽  
Continuous Light ◽  
Light Conditions ◽  
Wild Type ◽  
Photoautotrophic Growth ◽  
Light Regimen ◽  
Synechocystis Sp ◽  
Pcc 6803

ABSTRACT A mutation was recovered in the slr0721 gene, which encodes the decarboxylating NADP+-dependent malic enzyme in the cyanobacterium Synechocystis sp. strain PCC 6803, yielding the mutant 3WEZ. Under continuous light, 3WEZ exhibits poor photoautotrophic growth while growing photoheterotrophically on glucose at rates nearly indistinguishable from wild-type rates. Interestingly, under diurnal light conditions (12 h of light and 12 h of dark), normal photoautotrophic growth of the mutant is completely restored.

scholarly journals Free fatty acid production in the cyanobacterium Synechocystis sp. PCC 6803 is enhanced by deletion of the cyAbrB2 transcriptional regulator

2016 ◽  
Vol 220 ◽  
pp. 1-11 ◽  
Author(s):  
Akihito Kawahara ◽  
Yusuke Sato ◽  
Yujiro Saito ◽  
Yasuko Kaneko ◽  
Yasushi Takimura ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Free Fatty Acid ◽  
Acid Production ◽  
Transcriptional Regulator ◽  
Fatty Acid Production ◽  
Free Fatty Acid Production ◽  
Synechocystis Sp ◽  
Pcc 6803

Interrelation between Cyanophycin Synthesis, L-Arginine Catabolism and Photosynthesis in the Cyanobacterium Synechocystis Sp. Strain PCC 6803

2000 ◽  
Vol 55 (11-12) ◽  
pp. 927-942 ◽  
Author(s):  
Dirk Paul Stephan ◽  
Hans Georg Ruppel ◽  
Elfriede K. Pistorius
Keyword(s):  
Relative Concentration ◽  
Nutrient Deficiency ◽  
Phosphate Limitation ◽  
Physiological Changes ◽  
Wild Type ◽  
Synechocystis Pcc 6803 ◽  
N Source ◽  
Arginine Catabolism ◽  
Synechocystis Sp ◽  
Pcc 6803

ʟ Ultrastructural and imm unocytochemical investigations gave evidence that cyanophycin (multi--arginyl-poly-ʟ-aspartate) granules accumulate in the cyanobacterium Synechocystis sp. strain PCC 6803 under nutrient deficient growth conditions, especially under phosphate limitation. Besides nutrient deficiency, growth of Synechocystis PCC 6803 on ʟ-arginine or ʟ-asparagine as sole N-source also led to high increase of cyanophycin synthesis, while growth on the combination of ʟ-arginine or ʟ-asparagine with nitrate only caused minor cyanophycin accum ulation. Growth of Synechocystis PCC 6803 on ʟ-arginine as sole N-source caused substantial morphological and physiological changes, such as severe thylakoid membrane degradation with partial loss of pigments and photosynthetic activity leading to a phenotype almost like that seen under nutrient deficiency. In contrast to the wild type, the PsbO-free Synechocystis PCC 6803 mutant could grow on ʟ-arginine as sole N-source with only minor morphological and physiological changes. Due to its fairly balanced growth, the mutant accumulated only few cyanophycin granules. ʟ-arginine degrading activity (measured as ornithine and ammonium formation) was high in the PsbO-free mutant but not in the wild type when cells were grown on ʟ-arginine as sole N-source. In both cells types the ʟ-arginine degrading activity was high (although in the PsbO-free mutant about twice as high as in wild type), when cells were grown on ʟ-arginine in combination with nitrate, and as expected very low when cells were grown on nitrate as sole N-source. Thus, net cyanophycin accumulation in Synechocystis PCC 6803 is regulated by the relative concentration of ʟ-arginine to the total nitrogen pool, and the intracellular ʟ-arginine concentration is greatly influenced by the activity of the ʟ-arginine degrading enzyme system which in part is regulated by the activity status of photosystem II. These results suggest a complex interrelation between cyanophycin synthesis, ʟ-arginine catabolism , and in addition photosynthesis in Synechocystis PCC 6803.

scholarly journals The Mutant of sll1961, Which Encodes a Putative Transcriptional Regulator, Has a Defect in Regulation of Photosystem Stoichiometry in the Cyanobacterium Synechocystis sp. PCC 6803

2005 ◽  
Vol 139 (1) ◽  
pp. 408-416 ◽  
Author(s):  
Tamaki Fujimori ◽  
Mieko Higuchi ◽  
Hanayo Sato ◽  
Hiroshi Aiba ◽  
Masayuki Muramatsu ◽  
...  
Keyword(s):  
Transcriptional Regulator ◽  
Photosystem Stoichiometry ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Reduction of Photoautotrophic Productivity in the Cyanobacterium Synechocystis sp. Strain PCC 6803 by Phycobilisome Antenna Truncation

2012 ◽  
Vol 78 (17) ◽  
pp. 6349-6351 ◽  
Author(s):  
Lawrence E. Page ◽  
Michelle Liberton ◽  
Himadri B. Pakrasi
Keyword(s):  
Light Harvesting ◽  
Wild Type ◽  
Content Type ◽  
Photosynthetic Productivity ◽  
Mutant Strains ◽  
Light Harvesting Antenna ◽  
Culture Productivity ◽  
Synechocystis Sp ◽  
Pcc 6803

ABSTRACTTruncation of the algal light-harvesting antenna is expected to enhance photosynthetic productivity. The wild type and three mutant strains ofSynechocystissp. strain 6803 with a progressively smaller phycobilisome antenna were examined under different light and CO2conditions. Surprisingly, such antenna truncation resulted in decreased whole-culture productivity for this cyanobacterium.

Evidence for the production of a proteinaceous hemolytic exotoxin by wild-type strain of Synechocystis sp. PCC 6803 (Cyanobacteria)

2007 ◽  
Vol 20 (1) ◽  
pp. 89-95 ◽  
Author(s):  
Yunzhang Liu ◽  
Wei Wang ◽  
Shaoguo Ru ◽  
Yuanyuan Zhao
Keyword(s):  
Type Strain ◽  
Wild Type Strain ◽  
Wild Type ◽  
Synechocystis Sp ◽  
Pcc 6803

Phenotypic variation in wild-type substrains of the model cyanobacterium Synechocystis sp. PCC 6803

2016 ◽  
Vol 55 (1) ◽  
pp. 25-35 ◽  
Author(s):  
J. N. Morris ◽  
J. J. Eaton-Rye ◽  
T. C. Summerfield
Keyword(s):  
Phenotypic Variation ◽  
Wild Type ◽  
Synechocystis Sp ◽  
Pcc 6803
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