scholarly journals The Malic Enzyme Is Required for Optimal Photoautotrophic Growth of Synechocystis sp. Strain PCC 6803 under Continuous Light but Not under a Diurnal Light Regimen

2004 ◽  
Vol 186 (23) ◽  
pp. 8144-8148 ◽  
Author(s):  
Terry M. Bricker ◽  
Shulu Zhang ◽  
Susan M. Laborde ◽  
Paul R. Mayer ◽  
Laurie K. Frankel ◽  
...  
Keyword(s):  
Malic Enzyme ◽  
Continuous Light ◽  
Light Conditions ◽  
Wild Type ◽  
Photoautotrophic Growth ◽  
Light Regimen ◽  
Synechocystis Sp ◽  
Pcc 6803

ABSTRACT A mutation was recovered in the slr0721 gene, which encodes the decarboxylating NADP+-dependent malic enzyme in the cyanobacterium Synechocystis sp. strain PCC 6803, yielding the mutant 3WEZ. Under continuous light, 3WEZ exhibits poor photoautotrophic growth while growing photoheterotrophically on glucose at rates nearly indistinguishable from wild-type rates. Interestingly, under diurnal light conditions (12 h of light and 12 h of dark), normal photoautotrophic growth of the mutant is completely restored.

scholarly journals Four Novel Genes Required for Optimal Photoautotrophic Growth of the Cyanobacterium Synechocystis sp. Strain PCC 6803 Identified by In Vitro Transposon Mutagenesis

2004 ◽  
Vol 186 (3) ◽  
pp. 875-879 ◽  
Author(s):  
Shulu Zhang ◽  
Susan M. Laborde ◽  
Laurie K. Frankel ◽  
Terry M. Bricker
Keyword(s):  
Coat Protein ◽  
Malic Enzyme ◽  
Transposon Mutagenesis ◽  
Hypothetical Proteins ◽  
Photoautotrophic Growth ◽  
First Report ◽  
In Vitro Transposon Mutagenesis ◽  
Synechocystis Sp ◽  
Pcc 6803

ABSTRACT Four novel Synechocystis sp. strain PCC 6803 genes (sll1495, sll0804, slr1306, and slr1125) which encode hypothetical proteins were determined by transposon mutagenesis to be required for optimal photoautotrophic growth. Mutations were also recovered in ccmK4, a carboxysome coat protein homologue, and me, the decarboxylating NADP+-dependent malic enzyme. This is the first report that these known genes are required for optimal photoautotrophy.

scholarly journals Suppressor Mutations in the Study of Photosystem I Biogenesis: sll0088 Is a Previously Unidentified Gene Involved in Reaction Center Accumulation in Synechocystis sp. Strain PCC 6803

2003 ◽  
Vol 185 (13) ◽  
pp. 3878-3887 ◽  
Author(s):  
Jianping Yu ◽  
Gaozhong Shen ◽  
Tao Wang ◽  
Donald A. Bryant ◽  
John H. Golbeck ◽  
...  
Keyword(s):  
Photosystem I ◽  
Point Mutations ◽  
Kanamycin Resistance ◽  
Negative Regulator ◽  
Binding Motif ◽  
Wild Type ◽  
Photoautotrophic Growth ◽  
Suppressor Mutations ◽  
Mutant Strains ◽  
Pcc 6803

ABSTRACT In previous work, some members of our group isolated mutant strains of Synechocystis sp. strain PCC 6803 in which point mutations had been inserted into the psaC gene to alter the cysteine residues to the FA and FB iron-sulfur clusters in the PsaC subunit of photosystem I (J. P. Yu, I. R. Vassiliev, Y. S. Jung, J. H. Golbeck, and L. McIntosh, J. Biol. Chem. 272:8032-8039, 1997). These mutant strains did not grow photoautotrophically due to suppressed levels of chlorophyll a and photosystem I. In the results described here, we show that suppressor mutations produced strains that are capable of photoautotrophic growth at moderate light intensity (20 μmol m−2 s−1). Two separate suppressor strains of C14SPsaC, termed C14SPsaC-R62 and C14SPsaC-R18, were studied and found to have mutations in a previously uncharacterized open reading frame of the Synechocystis sp. strain PCC 6803 genome named sll0088. C14SPsaC-R62 was found to substitute Pro for Arg at residue 161 as the result of a G482→C change in sll0088, and C14SPsaC-R18 was found to have a three-amino-acid insertion of Gly-Tyr-Phe following Cys231 as the result of a TGGTTATTT duplication at T690 in sll0088. These suppressor strains showed near-wild-type levels of chlorophyll a and photosystem I, yet the serine oxygen ligand to FB was retained as shown by the retention of the S ≥ 3/2 spin state of the [4Fe-4S] cluster. The inactivation of sll0088 by insertion of a kanamycin resistance cartridge in the primary C14SPsaC mutant produced an engineered suppressor strain capable of photoautotrophic growth. There was no difference in psaC gene expression or in the amount of PsaC protein assembled in thylakoids between the wild type and an sll0088 deletion mutant. The sll0088 gene encodes a protein predicted to be a transcriptional regulator with sequence similarities to transcription factors in other prokaryotic and eukaryotic organisms, including Arabidopsis thaliana. The protein contains a typical helix-turn-helix DNA-binding motif and can be classified as a negative regulator by phylogenetic analysis. This suggests that the product of sll0088 has a role in regulating the biogenesis of photosystem I.

Interrelation between Cyanophycin Synthesis, L-Arginine Catabolism and Photosynthesis in the Cyanobacterium Synechocystis Sp. Strain PCC 6803

2000 ◽  
Vol 55 (11-12) ◽  
pp. 927-942 ◽  
Author(s):  
Dirk Paul Stephan ◽  
Hans Georg Ruppel ◽  
Elfriede K. Pistorius
Keyword(s):  
Relative Concentration ◽  
Nutrient Deficiency ◽  
Phosphate Limitation ◽  
Physiological Changes ◽  
Wild Type ◽  
Synechocystis Pcc 6803 ◽  
N Source ◽  
Arginine Catabolism ◽  
Synechocystis Sp ◽  
Pcc 6803

ʟ Ultrastructural and imm unocytochemical investigations gave evidence that cyanophycin (multi--arginyl-poly-ʟ-aspartate) granules accumulate in the cyanobacterium Synechocystis sp. strain PCC 6803 under nutrient deficient growth conditions, especially under phosphate limitation. Besides nutrient deficiency, growth of Synechocystis PCC 6803 on ʟ-arginine or ʟ-asparagine as sole N-source also led to high increase of cyanophycin synthesis, while growth on the combination of ʟ-arginine or ʟ-asparagine with nitrate only caused minor cyanophycin accum ulation. Growth of Synechocystis PCC 6803 on ʟ-arginine as sole N-source caused substantial morphological and physiological changes, such as severe thylakoid membrane degradation with partial loss of pigments and photosynthetic activity leading to a phenotype almost like that seen under nutrient deficiency. In contrast to the wild type, the PsbO-free Synechocystis PCC 6803 mutant could grow on ʟ-arginine as sole N-source with only minor morphological and physiological changes. Due to its fairly balanced growth, the mutant accumulated only few cyanophycin granules. ʟ-arginine degrading activity (measured as ornithine and ammonium formation) was high in the PsbO-free mutant but not in the wild type when cells were grown on ʟ-arginine as sole N-source. In both cells types the ʟ-arginine degrading activity was high (although in the PsbO-free mutant about twice as high as in wild type), when cells were grown on ʟ-arginine in combination with nitrate, and as expected very low when cells were grown on nitrate as sole N-source. Thus, net cyanophycin accumulation in Synechocystis PCC 6803 is regulated by the relative concentration of ʟ-arginine to the total nitrogen pool, and the intracellular ʟ-arginine concentration is greatly influenced by the activity of the ʟ-arginine degrading enzyme system which in part is regulated by the activity status of photosystem II. These results suggest a complex interrelation between cyanophycin synthesis, ʟ-arginine catabolism , and in addition photosynthesis in Synechocystis PCC 6803.

scholarly journals Reduction of Photoautotrophic Productivity in the Cyanobacterium Synechocystis sp. Strain PCC 6803 by Phycobilisome Antenna Truncation

2012 ◽  
Vol 78 (17) ◽  
pp. 6349-6351 ◽  
Author(s):  
Lawrence E. Page ◽  
Michelle Liberton ◽  
Himadri B. Pakrasi
Keyword(s):  
Light Harvesting ◽  
Wild Type ◽  
Content Type ◽  
Photosynthetic Productivity ◽  
Mutant Strains ◽  
Light Harvesting Antenna ◽  
Culture Productivity ◽  
Synechocystis Sp ◽  
Pcc 6803

ABSTRACTTruncation of the algal light-harvesting antenna is expected to enhance photosynthetic productivity. The wild type and three mutant strains ofSynechocystissp. strain 6803 with a progressively smaller phycobilisome antenna were examined under different light and CO2conditions. Surprisingly, such antenna truncation resulted in decreased whole-culture productivity for this cyanobacterium.

scholarly journals Malic Enzyme Facilitates d-Lactate Production through Increased Pyruvate Supply during Anoxic Dark Fermentation in Synechocystis sp. PCC 6803

2020 ◽  
Vol 9 (2) ◽  
pp. 260-268 ◽  
Author(s):  
Ryota Hidese ◽  
Mami Matsuda ◽  
Takashi Osanai ◽  
Tomohisa Hasunuma ◽  
Akihiko Kondo
Keyword(s):  
Malic Enzyme ◽  
Lactate Production ◽  
Dark Fermentation ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals RpaA Regulates the Accumulation of Monomeric Photosystem I and PsbA under High Light Conditions in Synechocystis sp. PCC 6803

PLoS ONE ◽  
2012 ◽  
Vol 7 (9) ◽  
pp. e45139 ◽  
Author(s):  
Waqar Majeed ◽  
Yan Zhang ◽  
Yong Xue ◽  
Saurabh Ranade ◽  
Ryan Nastashia Blue ◽  
...  
Keyword(s):  
Photosystem I ◽  
High Light ◽  
Light Conditions ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Diel light:dark cycles significantly reduce FFA accumulation in FFA producing mutants of Synechocystis sp. PCC 6803 compared to continuous light

2015 ◽  
Vol 12 ◽  
pp. 487-496 ◽  
Author(s):  
Yi Ern Cheah ◽  
Allison J. Zimont ◽  
Sunny K. Lunka ◽  
Stevan C. Albers ◽  
Sei Jin Park ◽  
...  
Keyword(s):  
Continuous Light ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Maximizing PHB content in Synechocystis sp. PCC 6803: development of a new photosynthetic overproduction strain

2020 ◽  
Author(s):  
Moritz Koch ◽  
Jonas Bruckmoser ◽  
Jörg Scholl ◽  
Waldemar Hauf ◽  
Bernhard Rieger ◽  
...  
Keyword(s):  
Industrial Production ◽  
Regulatory Protein ◽  
Continuous Light ◽  
Dry Weight ◽  
History Of ◽  
Model Strain ◽  
Synechocystis Sp ◽  
Rapid Conversion ◽  
Production Strain ◽  
Pcc 6803

AbstractPHB (poly-hydroxy-butyrate) represents a promising bioplastic variety with good biodegradation properties. Furthermore, PHB can be produced completely carbon-neutral when synthesized in the natural producer cyanobacterium Synechocystis sp. PCC 6803. This model strain has a long history of various attempts to further boost its low amounts of produced intracellular PHB of ~15 % per cell-dry-weight (CDW).We have created a new strain that lacks the regulatory protein PirC (gene product of sll0944), which causes a rapid conversion of the intracellular glycogen pools to PHB under nutrient limiting conditions. To further improve the intracellular PHB content, two genes from the PHB metabolism, phaA and phaB from the known production strain Cupriavidus necator, were introduced under the regime of the strong promotor PpsbA2. The created strain, termed PPT1 (Δsll0944-REphaAB), produced high amounts of PHB under continuous light as well under day-night rhythm. When grown in nitrogen and phosphor depleted medium, the cells produced up to 63 % / CDW. Upon the addition of acetate, the content was further increased to 81 % / CDW. The produced polymer consists of pure PHB, which is highly isotactic.The achieved amounts were the highest ever reported in any known cyanobacterium and demonstrate the potential of cyanobacteria for a sustainable, industrial production of PHB.

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