scholarly journals Toxic Cyanobacterial Bloom Triggers in Missisquoi Bay, Lake Champlain, as Determined by Next-Generation Sequencing and Quantitative PCR

Life ◽  
2015 ◽  
Vol 5 (2) ◽  
pp. 1346-1380 ◽  
Author(s):  
Nathalie Fortin ◽  
Valentina Munoz-Ramos ◽  
David Bird ◽  
Benoît Lévesque ◽  
Lyle Whyte ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Quantitative Pcr ◽  
Cyanobacterial Bloom ◽  
Next Generation ◽  
Lake Champlain ◽  
Generation Sequencing

scholarly journals Digital PCR—An Emerging Technology with Broad Applications in Microbiology

2019 ◽  
Vol 66 (1) ◽  
pp. 117-123 ◽  
Author(s):  
Stephen J Salipante ◽  
Keith R Jerome
Keyword(s):  
Next Generation Sequencing ◽  
Quantitative Pcr ◽  
Clinical Microbiology ◽  
Clinical Laboratory ◽  
Digital Pcr ◽  
Emerging Technology ◽  
Next Generation ◽  
Powerful Technique ◽  
Advantages And Disadvantages ◽  
Generation Sequencing

Abstract BACKGROUND The PCR and its variant, quantitative PCR (qPCR), have revolutionized the practice of clinical microbiology. Continued advancements in PCR have led to a new derivative, digital PCR (dPCR), which promises to address certain limitations inherent to qPCR. CONTENT Here we highlight the important technical differences between qPCR and dPCR, and the potential advantages and disadvantages of each. We then review specific situations in which dPCR has been implemented in clinical microbiology and the results of such applications. Finally, we attempt to place dPCR in the context of other emerging technologies relevant to the clinical laboratory, including next-generation sequencing. SUMMARY dPCR offers certain clear advantages over traditional qPCR, but these are to some degree offset by limitations of the technology, at least as currently practiced. Laboratories considering implementation of dPCR should carefully weigh the potential advantages and disadvantages of this powerful technique for each specific application planned.

scholarly journals Novel PCR-Based Methods Enhance Characterization of Vaginal Microbiota in a Bacterial Vaginosis Patient before and after Treatment

2013 ◽  
Vol 79 (13) ◽  
pp. 4181-4185 ◽  
Author(s):  
Janet A. Lambert ◽  
Apoorv Kalra ◽  
Cristina T. Dodge ◽  
Susan John ◽  
Jack D. Sobel ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Bacterial Vaginosis ◽  
Quantitative Pcr ◽  
Broad Spectrum ◽  
Vaginal Microbiota ◽  
Next Generation ◽  
Healthy Women ◽  
Before And After ◽  
Generation Sequencing

ABSTRACTDeep characterization, even by next-generation sequencing, of the vaginal microbiota in healthy women or posttreatment bacterial vaginosis patients is limited by the dominance of lactobacilli. To improve detection, we offer two approaches: quantitative PCR (qPCR) using phylogenetic branch-inclusive primers and sequencing of broad-spectrum amplicons generated with oligomers that block amplification of lactobacilli.

scholarly journals Minimal Residual Disease Detection by Next-Generation Sequencing in Multiple Myeloma: A Comparison With Real-Time Quantitative PCR

2021 ◽  
Vol 10 ◽  
Author(s):  
Qiumei Yao ◽  
Yinlei Bai ◽  
Shaji Kumar ◽  
Elaine Au ◽  
Alberto Orfao ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Next Generation Sequencing ◽  
Real Time ◽  
Quantitative Pcr ◽  
Residual Disease ◽  
Next Generation ◽  
Real Time Quantitative Pcr ◽  
Allele Specific ◽  
Next Generation Sequencing Ngs ◽  
Generation Sequencing

Here we compared clonotype identification by allele-specific oligonucleotide real-time quantitative-PCR (ASO RQ-PCR) and next-generation sequencing (NGS) in 80 multiple myeloma patients. ASO RQ-PCR was applicable in 49/55 (89%) and NGS in 62/78 (80%). Clonotypes identified by both methods were identical in 33/35 (94%). Sensitivity of 10−5 was confirmed in 28/29 (96%) by NGS while sensitivity of RQ-PCR was 10−5 in 7 (24%), 5 × 10−5 in 15 (52%), and 10−4 in 7 (24%). Among 14 samples quantifiable by ASO RQ-PCR, NGS yielded comparable results in 12 (86%). Applicability of NGS can be improved if immunoglobulin heavy-chain incomplete DJ primers are included.

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