Cell-Free Demineralized Bone Matrix for Mesenchymal Stem Cells Survival and Colonization

Decellularized bone matrix is receiving much attention as biological scaffolds and implantable biomaterials for bone tissue regeneration. Here, we evaluated the efficacy of a cell-free demineralized bone matrix on mesenchymal stem cells (MSCs) survival and differentiation in vitro. The seeding of human umbilical cord-derived MSCs (hUC-SCs) on decellularized bone matrices up to 14 days was exploited, assessing their capability of scaffold colonization and evaluating gene expression of bone markers. Light and Scanning Electron Microscopies were used. The obtained cell-free decalcified structures showed elastic moduli attributable to both topology and biochemical composition. Morphological observation evidenced an almost complete colonization of the scaffolds after 14 days of culture. Moreover, in hUC-SCs cultured on decalcified scaffolds, without the addition of any osteoinductive media, there was an upregulation of Collagen Type I (COL1) and osteonectin (ON) gene expression, especially on day 14. Modifications in the expression of genes engaged in stemness were also detected. In conclusion, the proposed decellularized bone matrix can induce the in vitro hUC-SCs differentiation and has the potential to be tested for in in vivo tissue regeneration.

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Implant Composed of Demineralized Bone and Mesenchymal Stem Cells Genetically Modified with AdBMP2/AdBMP7 for the Regeneration of Bone Fractures inOvis aries

Stem Cells International ◽

10.1155/2016/7403890 ◽

2016 ◽

Vol 2016 ◽

pp. 1-12 ◽

Cited By ~ 9

Author(s):

Adelina A. Hernandez-Hurtado ◽

Gissela Borrego-Soto ◽

Ivan A. Marino-Martinez ◽

Jorge Lara-Arias ◽

Viktor J. Romero-Diaz ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Bone Fractures ◽

Demineralized Bone Matrix ◽

Bone Matrix ◽

Adenoviral Vectors ◽

Bone Tissue Regeneration ◽

Demineralized Bone

Adipose-derived mesenchymal stem cells (ADMSCs) are inducible to an osteogenic phenotype by the bone morphogenetic proteins (BMPs). This facilitates the generation of implants for bone tissue regeneration. This study evaluated thein vitroosteogenic differentiation of ADMSCs transduced individually and in combination with adenoviral vectors expressing BMP2 and BMP7. Moreover, the effectiveness of the implant containing ADMSCs transduced with the adenoviral vectors AdBMP2/AdBMP7 and embedded in demineralized bone matrix (DBM) was tested in a model of tibial fracture in sheep. This graft was compared to ewes implanted with untransduced ADMSCs embedded in the same matrix and with injured but untreated animals.In vivoresults showed accelerated osteogenesis in the group treated with the AdBMP2/AdBMP7 transduced ADMSC graft, which also showed improved restoration of the normal bone morphology.

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Demineralized bone matrix combined with cytotoxic T‐lymphocyte associated protein 4 promotes osteogenic differentiation of human bone marrow mesenchymal stem cells and suppresses the activation of T lymphocytes in vitro

Journal of Tissue Engineering and Regenerative Medicine ◽

10.1002/term.3281 ◽

2021 ◽

Author(s):

Lei Song ◽

Rui Zhou ◽

Jun Xiao ◽

Lei He ◽

Fang Zhu ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

T Lymphocytes ◽

T Lymphocyte ◽

Cytotoxic T Lymphocyte ◽

Demineralized Bone Matrix ◽

Bone Matrix ◽

Marrow Mesenchymal Stem Cells

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3D Biomimetic Scaffold for Growth Factor Controlled Delivery: An In-Vitro Study of Tenogenic Events on Wharton’s Jelly Mesenchymal Stem Cells

Pharmaceutics ◽

10.3390/pharmaceutics13091448 ◽

2021 ◽

Vol 13 (9) ◽

pp. 1448

Author(s):

Maria Camilla Ciardulli ◽

Joseph Lovecchio ◽

Pasqualina Scala ◽

Erwin Pavel Lamparelli ◽

Tina Patricia Dale ◽

...

Keyword(s):

Gene Expression ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Type I Collagen ◽

In Vitro Study ◽

Wharton’S Jelly ◽

Controlled Delivery ◽

Type I ◽

Wharton's Jelly

The present work described a bio-functionalized 3D fibrous construct, as an interactive teno-inductive graft model to study tenogenic potential events of human mesenchymal stem cells collected from Wharton’s Jelly (hWJ-MSCs). The 3D-biomimetic and bioresorbable scaffold was functionalized with nanocarriers for the local controlled delivery of a teno-inductive factor, i.e., the human Growth Differentiation factor 5 (hGDF-5). Significant results in terms of gene expression were obtained. Namely, the up-regulation of Scleraxis (350-fold, p ≤ 0.05), type I Collagen (8-fold), Decorin (2.5-fold), and Tenascin-C (1.3-fold) was detected at day 14; on the other hand, when hGDF-5 was supplemented in the external medium only (in absence of nanocarriers), a limited effect on gene expression was evident. Teno-inductive environment also induced pro-inflammatory, (IL-6 (1.6-fold), TNF (45-fold, p ≤ 0.001), and IL-12A (1.4-fold)), and anti-inflammatory (IL-10 (120-fold) and TGF-β1 (1.8-fold)) cytokine expression upregulation at day 14. The presented 3D construct opens perspectives for the study of drug controlled delivery devices to promote teno-regenerative events.

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