scholarly journals Mineral Trioxide Aggregate (MTA) Upregulates the Expression of DMP1 in Direct Pulp Capping in the Rat Molar

Materials ◽  
2021 ◽  
Vol 14 (16) ◽  
pp. 4640
Author(s):  
Maiko Yamada ◽  
Motohiko Nagayama ◽  
Yuka Miyamoto ◽  
Satoshi Kawano ◽  
Yoshiaki Takitani ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mrna Expression ◽  
Calcium Hydroxide ◽  
Early Phase ◽  
Platelet Rich Plasma ◽  
Mineral Trioxide Aggregate ◽  
Direct Pulp Capping ◽  
Mineral Density ◽  
Pulp Capping ◽  
Male Wistar Rats

Mineral trioxide aggregate (MTA) is an alternative endodontic material that predicts conductive or inductive calcified tissue formation from immature pulp mesenchymal stem cells (IPMSCs). The purpose of this study was to investigate whether MTA could promote reparative odontoblast differentiation via IPMSCs in the early phase of regeneration and compare with calcium hydroxide (CH). Direct pulp capping using calcium hydroxide (CH), MTA, and MTA with platelet-rich plasma (MTA + PRP) was performed on maxillary first molars of 8-week-old male Wistar rats (n = 36). After 3, 7, or 14 days, the teeth were analyzed for mineral density (MD) and volume of MD (VMD) via micro-focusing computed tomography (µCT), nestin, dentin matrix acidic phosphoprotein 1 (DMP1) immunohistochemistry, and real-time PCR for DMP1 mRNA expression. MTA stimulated the early phase differentiation of the IPMSCs into odontoblasts, with positive results for nestin and DMP1 compared with CH. Moreover, MTA + PRP stimulated calcified granule and dentin bridge formation through calcium mineral deposition, following the induction of DMP1 mRNA expression in IPMSCs. Our results suggested that the combination of MTA and PRP is an effective and clinically applicable method for activating endogenous dental pulp stem cells into odontoblasts in the early stages of pulp regeneration.

scholarly journals Temperature changes in the pulp chamber induced by polymerization of resin-based dental restoratives following simulated direct pulp capping

2019 ◽  
Vol 73 (4) ◽  
pp. 239-248
Author(s):  
Violeta Petrovic ◽  
Jovana Stasic ◽  
Vojislav Komlenic ◽  
Tatjana Savic-Stankovic ◽  
Marina Latkovic ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Mineral Trioxide Aggregate ◽  
Glass Ionomer ◽  
Direct Pulp Capping ◽  
Pulp Chamber ◽  
Temperature Changes ◽  
Pulp Capping ◽  
Dental Restoratives ◽  
Resin Modified Glass Ionomer ◽  
Light Curing

The objective of this study was to measure temperature changes in the pulp chamber induced by polymerization of resin-based dental restoratives following a simulated procedure of direct pulp capping. Class I cavities with a microperforation at the pulp horn were prepared in extracted human molar teeth. The complete procedure of direct pulp capping and cavity restoration was performed with the root part of extracted teeth fixed in a water bath at 37 ?C. Mineral trioxide aggregate, bioactive dentin substitute or calcium-hydroxide paste were used as pulp capping materials. Cavities were restored with a light-cured or chemically-cured resin-modified glass ionomer, universal adhesive and a bulk-fill composite, cured with a high-intensity LED unit. Pulp capping materials caused a slight temperature decrease. Lower temperature increase was recorded during light-curing of the glass ionomer liner after direct capping with mineral trioxide aggregate and calcium-hydroxide than that recorded for the bioactive dentin substitute. Adhesive light-curing increased temperature in all groups with higher mean temperatures in groups with chemically-cured as compared to those for the light-cured glass ionomer liner. Direct pulp capping with mineral trioxide aggregate or calcium-hydroxide followed by the light-cured resin-modified glass ionomer liner and a bonded bulk-fill composite restoration induced temperature changes below the potentially adverse threshold of 42.5?C.

Direct Pulp Capping with Mineral Trioxide Aggregate: An Immunohistologic Comparison with Calcium Hydroxide in Rodents

2010 ◽  
Vol 36 (5) ◽  
pp. 814-819 ◽  
Author(s):  
Till Dammaschke ◽  
Udo Stratmann ◽  
Philipp Wolff ◽  
Darius Sagheri ◽  
Edgar Schäfer
Keyword(s):  
Calcium Hydroxide ◽  
Mineral Trioxide Aggregate ◽  
Direct Pulp Capping ◽  
Pulp Capping

Direct Pulp Capping with Calcium Hydroxide, Mineral Trioxide Aggregate, and Biodentine in Permanent Young Teeth with Caries: A Randomized Clinical Trial

2017 ◽  
Vol 43 (11) ◽  
pp. 1776-1780 ◽  
Author(s):  
Claudia Brizuela ◽  
Andrea Ormeño ◽  
Carolina Cabrera ◽  
Roxana Cabezas ◽  
Carolina Inostroza Silva ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Calcium Hydroxide ◽  
Randomized Clinical Trial ◽  
Mineral Trioxide Aggregate ◽  
Direct Pulp Capping ◽  
Pulp Capping

Treatment Outcome of Mineral Trioxide Aggregate or Calcium Hydroxide Direct Pulp Capping: Long-term Results

2014 ◽  
Vol 40 (11) ◽  
pp. 1746-1751 ◽  
Author(s):  
Johannes Mente ◽  
Sarah Hufnagel ◽  
Meltem Leo ◽  
Annemarie Michel ◽  
Holger Gehrig ◽  
...  
Keyword(s):  
Treatment Outcome ◽  
Calcium Hydroxide ◽  
Mineral Trioxide Aggregate ◽  
Long Term Results ◽  
Direct Pulp Capping ◽  
Pulp Capping

scholarly journals MTA and calcium hydroxide for pulp capping

2005 ◽  
Vol 13 (2) ◽  
pp. 126-130 ◽  
Author(s):  
Alexandra Mussolino de Queiroz ◽  
Sada Assed ◽  
Mario Roberto Leonardo ◽  
Paulo Nelson-Filho ◽  
Léa Assed Bezerra da Silva
Keyword(s):  
Calcium Hydroxide ◽  
Mineral Trioxide Aggregate ◽  
Inflammatory Cells ◽  
Direct Pulp Capping ◽  
Water Control ◽  
Pulp Tissue ◽  
Pulp Capping ◽  
Tissue Changes ◽  
Maxilla And Mandible ◽  
Direct Capping

This study evaluated the biocompatibility of mineral trioxide aggregate (MTA) after direct capping of exposed pulp tissue in dog's teeth. Class I cavities were prepared in 26 teeth from 3 adult dogs. MTA was applied over the exposed pulp in 13 teeth and paste of calcium hydroxide plus distilled water (control) was applied in the remaining 13 teeth. After 90 days, the animals were killed; the maxilla and mandible were dissected and sectioned to obtain individual roots. The samples were processed histologically. The pulp and periapical response observed with the use of MTA was similar to that of calcium hydroxide paste. In all specimens, there was a dentin bridge obliterating the exposure, an intact odontoblastic layer, no inflammatory cells, normal connective pulp tissue, normal apical and periapical regions and no bone tissue changes. Similar to calcium hydroxide, MTA presented excellent response when used for direct pulp capping.

scholarly journals Reparative Dentin Formation Using Stem Cell Therapy versus Calcium Hydroxide in Direct Pulp Capping: An Animal Study

2019 ◽  
Vol 30 (6) ◽  
pp. 542-549 ◽  
Author(s):  
Possy Abdelaz ◽  
Amira ElZoghbi ◽  
Mohamed Shokry ◽  
Abo-Zeid Ahmed ◽  
Hassan Rasha
Keyword(s):  
Stem Cells ◽  
Calcium Hydroxide ◽  
Elemental Analysis ◽  
Treated Group ◽  
Direct Pulp Capping ◽  
Isolated Cells ◽  
Electron Microscopic ◽  
Pulp Capping ◽  
Group I ◽  
Reparative Dentin

Abstract Direct pulp capping process is a therapeutic method aimed at maintenance of pulp vitality and health by using a biocompatible material placed directly over the exposed pulp. The aim of this study was to evaluate and compare the effect of direct pulp capping procedures by dental pulp stem cells (DPSCs) or calcium hydroxide on dentin tissue formation. Three mongrel dogs were used as experimental model. Two access cavities were prepared in the right and left mandibular fourth premolars in all dogs to expose and extirpate the pulp tissues which were processed in the lab to obtain a single-cell suspensions. The isolated cells were cultures in odontogenic medium for subsequent differentiation. The maxillary teeth (3 incisors and one canine) of the corresponding dog number were subjected to class V cavities to expose their pulps which were assigned into 2 groups of 12 teeth each ( group I - pulp capping with calcium hydroxide) and (group II - pulp capping with dental stem cells DPSCs). The operated teeth were collected after 3 months and processed for histological and electron microscopic examinations. Specimens were subjected to elemental analysis of calcium and phosphorus. EDX elemental analysis revealed significant differences in the calcium and phosphorous wt, % in the reparative dentin of calcium hydroxide treated group which confirmed histologically. Direct pulp capping by DPSCs has shown promising generative potential for regaining normal dentin.

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