scholarly journals A Novel Lipid Extraction Method from Wet Microalga Picochlorum sp. at Room Temperature

Marine Drugs ◽  
2014 ◽  
Vol 12 (3) ◽  
pp. 1258-1270 ◽  
Author(s):  
Fangfang Yang ◽  
Wenzhou Xiang ◽  
Xiumei Sun ◽  
Hualian Wu ◽  
Tao Li ◽  
...  
2003 ◽  
Vol 51 (19) ◽  
pp. 5607-5611 ◽  
Author(s):  
Sören Jensen ◽  
Lisbeth Häggberg ◽  
Hrönn Jörundsdóttir ◽  
Göran Odham

2012 ◽  
Vol 53 (8) ◽  
pp. 1690-1700 ◽  
Author(s):  
Lars Löfgren ◽  
Marcus Ståhlman ◽  
Gun-Britt Forsberg ◽  
Sinikka Saarinen ◽  
Ralf Nilsson ◽  
...  

1978 ◽  
Vol 24 (9) ◽  
pp. 1468-1472 ◽  
Author(s):  
T M Connolly ◽  
P Vecsei

Abstract We describe a simple radioimmunoassay of plasma cortisol, which can be performed in 3 h and which requires no purification, heating, or refrigeration steps. The plasma proteins are inhibited through direct competition between them and the antiserum at room temperature, at which the antiserum's affinity exceeds that of the binding proteins. Plasma, diluted with water, is incubated for 3 h at room temperature with [3H]cortisol and the antiserum. We compared results with those by the usual extraction method. The correlation between methods on evaluating normal samples with one antiserum. We compared results with those by the usual extraction method. The correlation between methods on evaluating normal samples with one antiserum was r = 0.954 (P less than 0.001), and the slope was 0.661. With three other antisera it was r = 0.922 (P less than 0.001) and slope 0.644. Plasmas with abnormal protein concentrations (i.e., from preganant women, and after corticotropin administration), tested to examine the validity of the method for routine use, and to define the role of the protein carriers, showed r = 0.859 (P less than 0.001) and slope 0.726 for the four antisera used. Additional samples, assayed with diluted standards plus stripped plasma, showed a correlation with the usual extraction method of r = 0.945 (P less than 0.001) and slope 1.026.


2003 ◽  
Vol 26 (6) ◽  
pp. 478-481 ◽  
Author(s):  
Changsuek Yon ◽  
Min Ho Lee ◽  
Doo -Yi Oh ◽  
Dal -Soo Kim ◽  
Ki Sung Lee ◽  
...  

2010 ◽  
Vol 65 (3-4) ◽  
pp. 266-270 ◽  
Author(s):  
Īsmail Poyraz ◽  
Emel Sözen ◽  
Muhittin Arslanyolu

We successfully used the guanidine isothiocyanate method for isolation of total RNA from leaf, stem, and root tissues of the aromatic plant Origanum onites. The RNA was extracted with TRI Reagent® at room temperature and was recovered by isopropanol precipitation. The isolated RNA was capable of reverse transcription. The extraction method described here does not require ultracentrifugation, and it is fast, simple, and effective. The procedure can be completed within 3 hours and may be applicable to other aromatic medicinal plants containing high amounts of phenolic compounds.


Lipids ◽  
2013 ◽  
Vol 48 (3) ◽  
pp. 307-318 ◽  
Author(s):  
Sarah K. Abbott ◽  
Andrew M. Jenner ◽  
Todd W. Mitchell ◽  
Simon H. J. Brown ◽  
Glenda M. Halliday ◽  
...  

2017 ◽  
Vol 71 (2) ◽  
pp. 167-174 ◽  
Author(s):  
Myung-Gyun Kim ◽  
Hyun-Wook Hwang ◽  
Antony Nzioka ◽  
Young-Ju Kim

In order to secure more effective lipid extraction method, this research investigated new lipid extraction method using laser with absorbent and sought its optimum operation control. In addition, this study compared lipid extraction efficiency and FAME conversion rate between laser extraction method at optimum condition and existing extraction method. Results from experiments for optimizing lipid extraction method using laser showed that the maximum extraction efficiency (81.8%) was attained when using laser with an output capacity of 75Wh/L. Extraction efficiency increased up to 90.8% when microwave treatment as pretreatment process was conducted. Addition of absorbents during lipid extraction process with laser showed higher extraction efficiency than laser and chemical method. It was also found that laser extraction method with absorbent had higher total fatty acid content (853.7 mg/g oil) in extracted lipid than chemical extraction method (825.4 mg/g oil). Furthermore, it had the highest FAME conversion rate (94.2%).


1976 ◽  
Vol 55 (3) ◽  
pp. 470-475 ◽  
Author(s):  
A.R. Johnson

Modification of a lipid extraction method was used to demonstrate the ability of calcium to stabilize salivary proteins in an aqueous solution. Gel electrophoretic patterns showed that slowly migrating components were most affected. Several biologically active factors are implicated to be among those stabilized.


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