Isolation of Quality Total RNA from the Aromatic Plant Origanum onites

2010 ◽  
Vol 65 (3-4) ◽  
pp. 266-270 ◽  
Author(s):  
Īsmail Poyraz ◽  
Emel Sözen ◽  
Muhittin Arslanyolu
Keyword(s):  
Phenolic Compounds ◽  
Medicinal Plants ◽  
Reverse Transcription ◽  
Extraction Method ◽  
Room Temperature ◽  
Aromatic Plant ◽  
Guanidine Isothiocyanate ◽  
Total Rna ◽  
Root Tissues ◽  
Origanum Onites

We successfully used the guanidine isothiocyanate method for isolation of total RNA from leaf, stem, and root tissues of the aromatic plant Origanum onites. The RNA was extracted with TRI Reagent® at room temperature and was recovered by isopropanol precipitation. The isolated RNA was capable of reverse transcription. The extraction method described here does not require ultracentrifugation, and it is fast, simple, and effective. The procedure can be completed within 3 hours and may be applicable to other aromatic medicinal plants containing high amounts of phenolic compounds.

scholarly journals HPLC Analysis of Chemical Composition of Selected Jordanian Medicinal Plants and Their Bioactive Properties

2018 ◽  
Vol 34 (5) ◽  
pp. 2397-2403
Author(s):  
Fuad Al-Rimawi ◽  
Fadi Alakhras ◽  
Wael A. Al-Zereini ◽  
Hammad K. Aldal'in ◽  
Saleh Abu-Lafi ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Phenolic Compounds ◽  
Medicinal Plants ◽  
Plant Extracts ◽  
Hplc Analysis ◽  
Room Temperature ◽  
Bacterial Strains ◽  
E Coli ◽  
Bioactive Properties

Three medicinal plants grown wild in Jordan, namely Achillea santolina L, Achillea fragrantisimma, Asteriscus graveolens (Forssk) Less, were extracted with ethyl acetate by continuous shaking at room temperature for three days. The antibacterial activity of the crude extract was evaluated. The extracts were analyzed for their phenolic and flavonoids content by HPLC-PDA. The HPLC analysis of the plant extracts revealed the presence of flavonoids and phenolic compounds in the three plant extracts. Results revealed a strong antibacterial activity of A. graveolens against three bacterial strains (B. subtilis, E. coli, and S.aureus) while A. fragrantissima inhibited the growth of B. subtilis. Bioactivities were attributed mainly to the immense content of phenol-based compounds in plants.

scholarly journals Identification and Antibacterial Evaluation of Selected Jordanian Medicinal Plants

2018 ◽  
Vol 34 (5) ◽  
pp. 2456-2463
Author(s):  
Wael A. Al-Zereini ◽  
Fuad Al-Rimawi ◽  
Saleh Abu-Lafi ◽  
Fadi Alakhras ◽  
Ghassab M. Al-Mazaideh ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Medicinal Plants ◽  
Ethyl Acetate ◽  
Plant Extracts ◽  
Crude Extract ◽  
Hplc Analysis ◽  
Room Temperature ◽  
Aerial Parts ◽  
Antibacterial Evaluation

Dried aerial parts of three medicinal plants grown wild in Jordan, namely Ononis natrix L., Salvia spinosa L. and Salvia verbenace L., were extracted upon soaking with ethyl acetate by continuous shaking at room temperature for three days. The extracts were analyzed for their phenolic and flavonoids content by HPLC-PDA. The HPLC analysis of the plant extracts revealed the presence of flavonoids and phenolic compounds in the three plant extracts. The antibacterial activity of the crude extract was evaluated. The PDA wavelengths range was from 227-347 nm. Bioactivities were attributed mainly to the immense content of phenol-based compounds in plants.

The content of phenolic compounds in medicinal plants of a botanical garden (Kaliningrad oblast)

2014 ◽  
Vol 41 (2) ◽  
pp. 133-138 ◽  
Author(s):  
P. V. Maslennikov ◽  
G. N. Chupakhina ◽  
L. N. Skrypnik
Keyword(s):  
Phenolic Compounds ◽  
Medicinal Plants ◽  
Botanical Garden ◽  
Kaliningrad Oblast

scholarly journals Changes in Elderberry (Sambucus nigra L.) Juice Concentrate Polyphenols during Storage

2021 ◽  
Vol 11 (15) ◽  
pp. 6941
Author(s):  
Cláudia M. B. Neves ◽  
António Pinto ◽  
Fernando Gonçalves ◽  
Dulcineia F. Wessel
Keyword(s):  
Phenolic Compounds ◽  
Chlorogenic Acid ◽  
Total Phenolic Content ◽  
Room Temperature ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Sambucus Nigra ◽  
Juice Concentrate ◽  
Rutin And Quercetin ◽  
Polymeric Color

Elderberry (Sambucus nigra L.) juice concentrate is highly rich in polyphenols, particularly anthocyanins and flavonols, which have been associated with a wide range of health-promoting properties. Phenolic compounds, in particular anthocyanins, are unstable and may change during storage, which might influence the product color quality and its potential health effects. The aim of this study was to evaluate the changes in the polyphenols profile of elderberry juice concentrate produced at an industrial scale during seven months of storage at 5 °C and at room temperature. The total phenolic content, the total monomeric anthocyanins, the percent polymeric color, and the ABTS•+ scavenging activity were monitored over time. In addition, the profile and content of the main individual phenolic compounds were also assessed by HPLC-DAD. The results show that cyanidin-3-O-sambubioside, cyanidin-3-O-glucoside, cyanidin-3-O-sambubioside-5-O-glucoside, cyanidin-3,5-O-diglucoside, chlorogenic acid, rutin, and quercetin-3-O-glucoside were the main phenolic compounds identified. Storage at room temperature resulted in a strong reduction in total monomeric anthocyanin content accompanied by an increase in percent polymeric color values. Cyanidin-3-O-sambubioside and cyanidin-3-O-glucoside degraded faster than cyanidin-3,5-O-diglucoside and cyanidin-3-O-sambubioside-5-O-glucoside. Concentration of chlorogenic acid also decreased over storage, whereas rutin and quercetin-3-O-glucoside were quite stable. Storage at 5 °C caused a lower impact on the contents of anthocyanins and chlorogenic acid and the percent polymeric color was not affected. The total phenolic content and the in vitro antioxidant activity remained quite similar over the time, for both temperatures, suggesting that elderberry concentrates still preserve their health benefits of antioxidant capacity after seven months of storage.

scholarly journals The Potential of Benefiting Variation between the Same Species of Artemisia Herba-alba from Different Location in Northeast of Libya

2019 ◽  
pp. 1-6
Author(s):  
Rania. F. M. Ali ◽  
Ahlam K. Alaila ◽  
Gebreel. A. A. Aldaaiek
Keyword(s):  
Phenolic Compounds ◽  
Medicinal Plants ◽  
Essential Oils ◽  
Total Protein ◽  
Mineral Content ◽  
The Other ◽  
Mineral Analysis ◽  
Phenolic Contents

The medicinal plants (Artemisia herba-alba) were subjected to mineral analysis, total protein and the phenolic contents. Couple plants were collected from AL-Gabal AL-Akhder region in Northeast of Libya (Coastal and Desert) Artemisia herba-alba 1 Artemisia herba-alba 2 respectively during November (2018).  Results showed that mineral content found to vary significantly. Appreciable amounts of calcium Ca was recorded 84.930 ppm in Artemisia herba-alba 1 while 30 ppm in Artemisia herba-alba 2, potassium (K) was 43.3 and 27.6 ppm in Artemisia herba-alba 1 and Artemisia herba-alba 2 respectively. Meanwhile, Fe was recorded as 0.39, 0.52 ppm in Artemisia herba-alba1 and 2. Level of total protein was 9.95 and 7.79 (mg/g) in Artemisia herba-alba 1 and Artemisia herba-alba 2 respectively. Present study found that the phenolic compounds were determined in both plants with high levels. The available data indicate the two plants were found to contain Alkaloids, essential oils, flavonoids, glycosides, phenolic compounds, sterols/triterpenes, and tannins. However, sterols/ triterpenes and coumarins were found in herba-alba 1  significantly, while Alkaloids, flavonoids, and Saponins found highest in herba-alba 2 compared to herba-alba 1 on the other side the quantity of essential oils was higher in the herba-alba 2.

scholarly journals Bioactive Phenolic Compounds from Indian Medicinal Plants for Pharmaceutical and Medical Aspects

2021 ◽  
Author(s):  
Bhanu Kumar ◽  
Ankita Misra ◽  
Sharad Srivastava
Keyword(s):  
Phenolic Compounds ◽  
Medicinal Plants ◽  
Modern Medicine ◽  
The Body ◽  
Human Welfare ◽  
Therapeutic Benefits ◽  
Plant Constituents ◽  
Health And Disease ◽  
Tools And Techniques ◽  
Indian Medicinal Plants

Ayurveda is an age old system of medicine which utilizes thousands of medicinal plants, rich in secondary metabolites for their therapeutic benefits and phenolic compounds are important one. Plant phenolic compounds are one of the major group of phytomolecules having tremendous therapeutic and nutraceutical potential. Indian medicinal plants like Emblica, Terminalia spp., Withania, Tinospora etc. are some of the potential source of bioactive phenolics and had been used from ages in various Ayurvedic formulations and were scientifically validated too. In this contribution, a brief account of some common Indian medicinal plants rich in bioactive phenolics are summarized along with their therapeutic action on human health and disease. The vast array of phenolics in these plants makes them a suitable candidate for modern medicine, nutraceutical supplements, immuno-modulatory formulations etc. With the advent of modern separation tools and techniques, it is now possible to identify, isolate and purify desired phytoconstituents from plant extracts. This further opens the avenues of utilizing medicinal plants or plant constituents/metabolites as super food for strengthening the body and maintaining the healthy work-life balance. The need of the hour is to identify therapeutically potential phenolics rich plants and development of herbal formulations for human welfare.

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