scholarly journals Simultaneous Quantification of Protein Binding Kinetics in Whole Cells with Surface Plasmon Resonance Imaging and Edge Deformation Tracking

Membranes ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 247
Author(s):  
Wenwen Jing ◽  
Ashley Hunt ◽  
Nongjian Tao ◽  
Fenni Zhang ◽  
Shaopeng Wang
Keyword(s):  
Surface Plasmon Resonance ◽  
Membrane Protein ◽  
Cell Surface ◽  
Protein Binding ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Binding Kinetics ◽  
Cell Edge ◽  
Edge Tracking ◽  
Spr Imaging

Most drugs work by binding to receptors on the cell surface. Quantification of binding kinetics between drug and membrane protein is an essential step in drug discovery. Current methods for measuring binding kinetics involve extracting the membrane protein and labeling, and both have issues. Surface plasmon resonance (SPR) imaging has been demonstrated for quantification of protein binding to cells with single-cell resolution, but it only senses the bottom of the cell and the signal diminishes with the molecule size. We have discovered that ligand binding to the cell surface is accompanied by a small cell membrane deformation, which can be used to measure the binding kinetics by tracking the cell edge deformation. Here, we report the first integration of SPR imaging and cell edge tracking methods in a single device, and we use lectin interaction as a model system to demonstrate the capability of the device. The integration enables the simultaneous collection of complementary information provided by both methods. Edge tracking provides the advantage of small molecule binding detection capability, while the SPR signal scales with the ligand mass and can quantify membrane protein density. The kinetic constants from the two methods were cross-validated and found to be in agreement at the single-cell level. The variation of observed rate constant between the two methods is about 0.009 s−1, which is about the same level as the cell-to-cell variations. This result confirms that both methods can be used to measure whole-cell binding kinetics, and the integration improves the reliability and capability of the measurement.

scholarly journals Trends in SPR Cytometry: Advances in Label-Free Detection of Cell Parameters

Biosensors ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 102 ◽  
Author(s):  
Richard Schasfoort ◽  
Fikri Abali ◽  
Ivan Stojanovic ◽  
Gestur Vidarsson ◽  
Leon Terstappen
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Mammalian Cells ◽  
Living Cells ◽  
Label Free ◽  
Intact Cells ◽  
Cell Parameters ◽  
Spr Imaging ◽  
Label Free Detection

SPR cytometry entails the measurement of parameters from intact cells using the surface plasmon resonance (SPR) phenomenon. Specific real-time and label-free binding of living cells to sensor surfaces has been made possible through the availability of SPR imaging (SPRi) instruments and researchers have started to explore its potential in the last decade. Here we will discuss the mechanisms of detection and additionally describe the problems and issues of mammalian cells in SPR biosensing, both from our own experience and with information from the literature. Finally, we build on the knowledge and applications that has already materialized in this field to give a forecast of some exciting applications for SPRi cytometry.

scholarly journals Study of Immobilization Procedure on Silver Nanolayers and Detection of Estrone with Diverged Beam Surface Plasmon Resonance (SPR) Imaging

Biosensors ◽  
2013 ◽  
Vol 3 (1) ◽  
pp. 157-170 ◽  
Author(s):  
Alina Karabchevsky ◽  
Lev Tsapovsky ◽  
Robert Marks ◽  
Ibrahim Abdulhalim
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Spr Imaging ◽  
Beam Surface

scholarly journals Binding Kinetics of Methylene Blue on Monolayer Graphene Investigated by Multiparameter Surface Plasmon Resonance

ACS Omega ◽  
2018 ◽  
Vol 3 (7) ◽  
pp. 7133-7140 ◽  
Author(s):  
Nur Selin Kaya ◽  
Anur Yadav ◽  
Michel Wehrhold ◽  
Laura Zuccaro ◽  
Kannan Balasubramanian
Keyword(s):  
Methylene Blue ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Binding Kinetics ◽  
Monolayer Graphene ◽  
Kinetics Of

Trends in the development of sensor devices based on surface plasmon resonance (Review)

2021 ◽  
Vol 56 ◽  
pp. 5-26
Author(s):  
A. V. Samoylov ◽  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmons ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Light Wave ◽  
Monochromatic Light ◽  
Dielectric Substrate ◽  
Spr Imaging ◽  
Imaging Sensors ◽  
Polarization Contrast

Trends in the development of modern sensory devices based on surface plasmon resonance (SPR) are considered. The basic principles of construction of SPR sensor are given. For excitation of surface plasmons on the surface of sensitive elements of biosensory, a prism of total internal reflection is used or a dielectric substrate are used. A thin (dozens nm) film of high-conductive metal (mainly gold or silver) is applied to the working surface of the prisms or dielectric substrate. In a typical observation experiment, SPR is measured dependence on the angle of increasing light intensity, reflected by the resonance sensitive surface of the prism (chip). The optical schemes and principles of work of various SPR sensors are considered: - SPR Sensors with angular modulation, which are the most commonly used method based on the corner registration, in which the SPR occurs. The surface of the metal film is irradiated by monochromatic light and scans on a certain range of angles. There is a kind of SPR sensors with angular modulation, in which there is no mechanical scan of the angle of fall. Such sensors are entirely necessary for excitation of PPRs a set of angles is obtained due to a divergent or convergent light beam. - PPR sensors with a wavelength modulation is based on fixing an angle of falling light at a certain value and modulation of the wavelength of the incident light. Excitation of surface plasmons leads to a characteristic failure in the spectrum of reflected radiation. - Phase sensitive SPR sensors in which a change in the phase of the light wave associated with the surface plasma is measured on one corner of the fall and the wavelength of the light wave and is used as the output signal. - SPR imaging sensors in which the Technology of SPR imaging (SPRi) combines the sensitivity of the SPR with spatial image capabilities. The SPRI circuit uses as a fixed angle (as a rule, a slightly left angle of the SPR) and a fixed wavelength to measure changes in the reflection ability (Δ% R) that occur when the curve of the SPR is shifted due to the change in the refractive index above the surface of the sensor element. - SPR imaging sensors polarization contrast. In order to improve the quality of high-performance SPR imaging sensors in terms of sensitivity and resolution, the method of polarization contrast is used Disadvantages and advantages of SPR sensors are constructed with different principles are considered. The design and prospect of the use of achromatic and suburchast wave plates in the PPR imaging sensors with polarization contrast are considered.

Real-Time Analysis of Ligand-Induced Cell Surface and Intracellular Reactions of Living Mast Cells Using a Surface Plasmon Resonance-Based Biosensor

2002 ◽  
Vol 302 (1) ◽  
pp. 28-37 ◽  
Author(s):  
Michihiro Hide ◽  
Tomoko Tsutsui ◽  
Hajime Sato ◽  
Tomoaki Nishimura ◽  
Kenichi Morimoto ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Mast Cells ◽  
Cell Surface ◽  
Real Time ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Time Analysis ◽  
Real Time Analysis

Surface plasmon resonance imaging for ABH antigen detection on red blood cells and in saliva: secretor status-related ABO subgroup identification

The Analyst ◽  
2017 ◽  
Vol 142 (9) ◽  
pp. 1471-1481 ◽  
Author(s):  
Patjaree Peungthum ◽  
Krisda Sudprasert ◽  
Ratthasart Amarit ◽  
Armote Somboonkaew ◽  
Boonsong Sutapun ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Blood Cells ◽  
Antibody Array ◽  
Surface Plasmon Resonance Imaging ◽  
Resonance Imaging ◽  
Secretor Status ◽  
Subgroup Identification ◽  
Spr Imaging

Fewer steps for ABO subgroup identification by using the SPR imaging incorporating an antibody array was presented.

scholarly journals Dynamic Dispersal of Surface Layer Biofilm Induced by Nanosized TiO 2 Based on Surface Plasmon Resonance and Waveguide

2018 ◽  
Vol 84 (9) ◽  
Author(s):  
Peng Zhang ◽  
Jin-Song Guo ◽  
Peng Yan ◽  
You-Peng Chen ◽  
Wei Wang ◽  
...  
Keyword(s):  
Surface Layer ◽  
Surface Plasmon Resonance ◽  
Cell Surface ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Bacterial Attachment ◽  
Short Period ◽  
Bacterial Migration ◽  
Adhesive Ability ◽  
Biofilm Dispersal

ABSTRACT Pollutant degradation is present mainly in the surface layer of biofilms, and the surface layer is the most vulnerable to impairment by toxic pollutants. In this work, the effects of nanosized TiO 2 (n-TiO 2 ) on the average thicknesses of Bacillus subtilis biofilm and on bacterial attachment on different surfaces were investigated. The binding mechanism of n-TiO 2 to the cell surface was also probed. The results revealed that n-TiO 2 caused biofilm dispersal and the thicknesses decreased by 2.0 to 2.6 μm after several hours of exposure. The attachment abilities of bacteria with extracellular polymeric substances (EPS) on hydrophilic surfaces were significantly reduced by 31% and 81% under 10 and 100 mg/liter of n-TiO 2 , respectively, whereas those of bacteria without EPS were significantly reduced by 43% and 87%, respectively. The attachment abilities of bacteria with and without EPS on hydrophobic surfaces were significantly reduced by 50% and 56%, respectively, under 100 mg/liter of n-TiO 2 . The results demonstrated that biofilm dispersal can be attributed to the changes in the cell surface structure and the reduction of microbial attachment ability. IMPORTANCE Nanoparticles can penetrate into the outer layer of biofilm in a relatively short period and can bind onto EPS and bacterial surfaces. The current work probed the effects of nanosized TiO 2 (n-TiO 2 ) on biofilm thickness, bacterial migration, and surface properties of the cell in the early stage using the surface plasmon resonance waveguide mode. The results demonstrated that n-TiO 2 decreased the adhesive ability of both cell and EPS and induced bacterial migration and biofilm detachment in several hours. The decreased adhesive ability of microbes and EPS worked against microbial aggregation, reducing the effluent quality in the biological wastewater treatment process.

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