scholarly journals Argon and Argon–Oxygen Plasma Surface Modification of Gelatin Nanofibers for Tissue Engineering Applications

Membranes ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 31
Author(s):  
Abolfazl Mozaffari ◽  
Mazeyar Parvinzadeh Gashti ◽  
Mohammad Mirjalili ◽  
Masoud Parsania
Keyword(s):  
Tissue Engineering ◽  
Surface Modification ◽  
Plasma Treatment ◽  
Ftir Spectroscopy ◽  
Oxygen Plasma ◽  
Attenuated Total Reflection ◽  
Oxygen Plasma Treatment ◽  
Engineering Applications ◽  
Plasma Surface Modification ◽  
Plasma Surface

In the present study, we developed a novel approach for functionalization of gelatin nanofibers using the plasma method for tissue engineering applications. For this purpose, tannic acid-crosslinked gelatin nanofibers were fabricated with electrospinning, followed by treatment with argon and argon–oxygen plasmas in a vacuum chamber. Samples were evaluated by using scanning electron microscopy (SEM), atomic force microscopy (AFM), attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy, contact angle (CA) and X-ray diffraction (XRD). The biological activity of plasma treated gelatin nanofibers were further investigated by using fibroblasts as cell models. SEM studies showed that the average diameter and the surface morphology of nanofibers did not change after plasma treatment. However, the mean surface roughness (RMS) of samples were increased due to plasma activation. ATR-FTIR spectroscopy demonstrated several new bands on plasma treated fibers related to the plasma ionization of nanofibers. The CA test results stated that the surface of nanofibers became completely hydrophilic after argon–oxygen plasma treatment. Finally, increasing the polarity of crosslinked gelatin after plasma treatment resulted in an increase of the number of fibroblast cells. Overall, results expressed that our developed method could open new insights into the application of the plasma process for functionalization of biomedical scaffolds. Moreover, the cooperative interplay between gelatin biomaterials and argon/argon–oxygen plasmas discovered a key composition showing promising biocompatibility towards biological cells. Therefore, we strongly recommend plasma surface modification of nanofiber scaffolds as a pretreatment process for tissue engineering applications.

Plasma surface modification technique–induced gelatin grafting on bio-originated polyurethane porous matrix: Physicochemical and in vitro study

2020 ◽  
pp. 096739112092907 ◽  
Author(s):  
Sahar M Hesari ◽  
Farimah Ghorbani ◽  
Farnaz Ghorbani ◽  
Ali Zamanian ◽  
Alireza Khavandi
Keyword(s):  
Fourier Transform ◽  
Surface Modification ◽  
Infrared Spectroscopy ◽  
Plasma Treatment ◽  
Fourier Transform Infrared Spectroscopy ◽  
Oxygen Plasma ◽  
Fourier Transform Infrared ◽  
Attenuated Total Reflection ◽  
Oxygen Plasma Treatment ◽  
Activated Surface

In this study, polyurethane (TPU) scaffolds were fabricated using freeze-drying technique and gelatin macromolecules immobilized on the activated surface by oxygen plasma treatment. Scanning electron microscopy (SEM) micrographs indicated an interconnected porous microstructure with randomly oriented pores. According to the results, the diameter of pores increased after plasma treatment and gelatin grafting. Fourier transform infrared spectroscopy illustrated that there is no inappropriate interaction between materials during processing; furthermore, attenuated total reflection Fourier transform infrared spectroscopy confirmed the immobilization of gelatin molecules on the surface of the plasma-treated polymeric scaffolds. Waterdrop contact angle analysis presented that wettability and hydrophilicity of constructs increased after grafting gelatin on the activated surface. Phosphate-buffered saline absorption and hydrolytic biodegradation enhanced after surface modification of the polymeric samples. Cellular behavior demonstrated better adhesion and spreading after grafting gelatin of oxygen plasma-treated constructs. No evidence of toxicity was observed for 7 days. DNA content determined that the number of viable cells increased in TPU-gelatin matrixes after 1 day in contrast with TPU scaffolds. Based on results, oxygen plasma treatment can create an activated surface to graft gelatin macromolecules and achieve optimum physicochemical, mechanical, and biological features for the neo-tissue formation.

scholarly journals Preparation of Silver-Plated Para-Aramid Fiber by Employing Low-Temperature Oxygen Plasma Treatment and Dopamine Functionalization

Coatings ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 599 ◽  
Author(s):  
Zhenhua Sun ◽  
Yanfen Zhou ◽  
Wenyue Li ◽  
Shaojuan Chen ◽  
Shihua You ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Silver Nanoparticles ◽  
Plasma Treatment ◽  
Electrical Resistance ◽  
Oxygen Plasma ◽  
Attenuated Total Reflection ◽  
Oxygen Plasma Treatment ◽  
Washing Fastness ◽  
Before And After ◽  
Electroless Silver Plating

Direct electroless silver plating of para-aramid (PPTA) is difficult due to its extremely low surface chemical energy. In order to facilitate the deposition of silver nanoparticles and to enhance the washing fastness, oxygen plasma treatment and dopamine modification were conducted before silver plating of PPTA fibers. Various techniques including scanning electron microscopy (SEM), attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffractometer (XRD) and thermogravimetric analyzer (TGA) were used to characterize the surface morphology, chemical composition and thermal stability of the silver-plated PPTA fibers. Electrical resistance and silver content of the silver-coated PPTA fibers before and after standard washing were also studied. The results showed that silver nanoparticles were successfully coated onto the surface of PPTA fibers with and without plasma treatment, but the coating continuity and the electrical conductivity of the silver-coated PPTA fibers were greatly enhanced with the assistance of plasma treatment. It was also demonstrated that the washing fastness of silver-coated PPTA fibers was improved after plasma treatment as indicated by electrical resistance and continuity of the silver nanoparticles after various washing cycles. It was found that the electrical resistance of plasma-treated PPTA-PDA/Ag fibers prepared at an AgNO3 concentration of 20 g/L reached 0.89 Ω/cm and increased slightly to 0.94 Ω/cm after 10 standard washing cycles. The silver-coated PPTA fibers also showed stable electrical conductivity under 250 repeated stretching-releasing cycles at a strain of 3%.

scholarly journals Oxygen plasma surface modification enhances immobilization of simvastatin acid

2006 ◽  
Vol 27 (1) ◽  
pp. 29-36 ◽  
Author(s):  
Masao YOSHINARI ◽  
Tohru HAYAKAWA ◽  
Kenichi MATSUZAKA ◽  
Takashi INOUE ◽  
Yutaka ODA ◽  
...  
Keyword(s):  
Surface Modification ◽  
Oxygen Plasma ◽  
Plasma Surface Modification ◽  
Plasma Surface ◽  
Simvastatin Acid

scholarly journals Surface modification of P dl LGA microspheres with gelatine methacrylate: Evaluation of adsorption, entrapment, and oxygen plasma treatment approaches

2017 ◽  
Vol 53 ◽  
pp. 450-459 ◽  
Author(s):  
Abdulrahman Baki ◽  
Cheryl V. Rahman ◽  
Lisa J. White ◽  
David J. Scurr ◽  
Omar Qutachi ◽  
...  
Keyword(s):  
Surface Modification ◽  
Plasma Treatment ◽  
Oxygen Plasma ◽  
Oxygen Plasma Treatment ◽  
Treatment Approaches

Physicochemical and biological investigation of oxygen plasma modified electrospun polyurethane scaffolds for connective tissue engineering application

2019 ◽  
Vol 39 (6) ◽  
pp. 526-533
Author(s):  
Farnaz Ghorbani ◽  
Ali Zamanian
Keyword(s):  
Tissue Engineering ◽  
Plasma Treatment ◽  
Oxygen Plasma ◽  
Fiber Diameter ◽  
Engineering Application ◽  
Biological Behavior ◽  
Plasma Modification ◽  
Diameter Distribution ◽  
Oxygen Plasma Treatment ◽  
Tissue Engineering Application

AbstractIn this study, electrospinning was selected to fabricate randomly oriented polyurethane (PU) nanofibers for tissue engineering application, and the surface of scaffolds was exposed to oxygen plasma flow. The morphology structure of the PU scaffolds before and after oxygen plasma treatment was observed using scanning electron microscopy (SEM) micrographs, and the fiber diameter distribution was measured using Image J software. The results demonstrated that oxygen plasma modification reduces the fiber diameter without any other special effects on fiber microstructure. Water drop contact angle and swelling ratio of PU constructs were performed to estimate the water-scaffolds interactions. The results revealed improvement of hydrophilicity by oxygen plasma treatment. Atomic force microscopy test was done to analyze a topological characteristic of the scaffolds, and it was found out that oxygen plasma treatment decreases the roughness of the scaffolds. The biological behavior of the scaffolds was investigated by SEM observation and MTT assay after L-929 fibroblast cells culture.In vitroassays demonstrated biocompatibility, cellular attachments, and filopodia formation on plasma modified samples. These results suggest that oxygen plasma treatment improves the physicochemical and biological properties of PU scaffolds to create a more hydrophilic surface which facilitates cell attachments and proliferation.

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