scholarly journals The Impact of Photorespiratory Glycolate Oxidase Activity on Arabidopsis thaliana Leaf Soluble Amino Acid Pool Sizes during Acclimation to Low Atmospheric CO2 Concentrations

Metabolites ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 501
Author(s):  
Younès Dellero ◽  
Caroline Mauve ◽  
Mathieu Jossier ◽  
Michael Hodges
Keyword(s):  
Amino Acids ◽  
Arabidopsis Thaliana ◽  
Amino Acid ◽  
Atmospheric Co2 ◽  
Glycolate Oxidase ◽  
Wild Type ◽  
Low Co2 ◽  
Co2 Concentrations ◽  
Atmospheric Co2 Concentrations ◽  
The Impact

Photorespiration is a metabolic process that removes toxic 2-phosphoglycolate produced by the oxygenase activity of ribulose-1,5-bisphosphate carboxylase/oxygenase. It is essential for plant growth under ambient air, and it can play an important role under stress conditions that reduce CO2 entry into the leaf thus enhancing photorespiration. The aim of the study was to determine the impact of photorespiration on Arabidopsis thaliana leaf amino acid metabolism under low atmospheric CO2 concentrations. To achieve this, wild-type plants and photorespiratory glycolate oxidase (gox) mutants were given either short-term (4 h) or long-term (1 to 8 d) low atmospheric CO2 concentration treatments and leaf amino acid levels were measured and analyzed. Low CO2 treatments rapidly decreased net CO2 assimilation rate and triggered a broad reconfiguration of soluble amino acids. The most significant changes involved photorespiratory Gly and Ser, aromatic and branched-chain amino acids as well as Ala, Asp, Asn, Arg, GABA and homoSer. While the Gly/Ser ratio increased in all Arabidopsis lines between air and low CO2 conditions, low CO2 conditions led to a higher increase in both Gly and Ser contents in gox1 and gox2.2 mutants when compared to wild-type and gox2.1 plants. Results are discussed with respect to potential limiting enzymatic steps with a special emphasis on photorespiratory aminotransferase activities and the complexity of photorespiration.

scholarly journals Heterogeneous CO<sub>2</sub> and CH<sub>4</sub> content of glacial meltwater from the Greenland Ice Sheet and implications for subglacial carbon processes

2021 ◽  
Vol 15 (3) ◽  
pp. 1627-1644
Author(s):  
Andrea J. Pain ◽  
Jonathan B. Martin ◽  
Ellen E. Martin ◽  
Åsa K. Rennermalm ◽  
Shaily Rahman
Keyword(s):  
Greenhouse Gas ◽  
Ice Sheet ◽  
Greenland Ice Sheet ◽  
Mineral Weathering ◽  
Atmospheric Co2 ◽  
The Arctic ◽  
Co2 Concentrations ◽  
Geochemical Models ◽  
Atmospheric Co2 Concentrations ◽  
The Impact

Abstract. Accelerated melting of the Greenland Ice Sheet has increased freshwater delivery to the Arctic Ocean and amplified the need to understand the impact of Greenland Ice Sheet meltwater on Arctic greenhouse gas budgets. We evaluate subglacial discharge from the Greenland Ice Sheet for carbon dioxide (CO2) and methane (CH4) concentrations and δ13C values and use geochemical models to evaluate subglacial CH4 and CO2 sources and sinks. We compare discharge from southwest (a sub-catchment of the Isunnguata Glacier, sub-Isunnguata, and the Russell Glacier) and southern Greenland (Kiattut Sermiat). Meltwater CH4 concentrations vary by orders of magnitude between sites and are saturated with respect to atmospheric concentrations at Kiattut Sermiat. In contrast, meltwaters from southwest sites are supersaturated, even though oxidation reduces CH4 concentrations by up to 50 % during periods of low discharge. CO2 concentrations range from supersaturated at sub-Isunnguata to undersaturated at Kiattut Sermiat. CO2 is consumed by mineral weathering throughout the melt season at all sites; however, differences in the magnitude of subglacial CO2 sources result in meltwaters that are either sources or sinks of atmospheric CO2. At the sub-Isunnguata site, the predominant source of CO2 is organic matter (OM) remineralization. However, multiple or heterogeneous subglacial CO2 sources maintain atmospheric CO2 concentrations at Russell but not at Kiattut Sermiat, where CO2 is undersaturated. These results highlight a previously unrecognized degree of heterogeneity in greenhouse gas dynamics under the Greenland Ice Sheet. Future work should constrain the extent and controls of heterogeneity to improve our understanding of the impact of Greenland Ice Sheet melt on Arctic greenhouse gas budgets, as well as the role of continental ice sheets in greenhouse gas variations over glacial–interglacial timescales.

Climate-induced hysteresis of the tropical forest in the fire-enabled Earth system model CM2Mc-LPJmL

2021 ◽  
Author(s):  
Markus Drüke ◽  
Werner v. Bloh ◽  
Boris Sakschewski ◽  
Nico Wunderling ◽  
Stefan Petri ◽  
...  
Keyword(s):  
Tropical Forest ◽  
Tropical Forests ◽  
Atmospheric Co2 ◽  
Forest Recovery ◽  
Surface Model ◽  
Original State ◽  
Co2 Concentrations ◽  
Impact Phase ◽  
Atmospheric Co2 Concentrations ◽  
The Impact

&lt;p&gt;Tropical rainforests are recognized as one of the terrestrial tipping elements which could have profound impacts on the global climate, once their vegetation has transitioned into savanna or grassland states. While several studies investigated the savannization of, e.g., the Amazon rainforest, few studies considered the influence of fire. Fire is expected to potentially shift the savanna-forest boundary and hence impact the dynamical equilibrium between these two possible vegetation states under changing climate. To investigate the climate-induced hysteresis in pan-tropical forests and the impact of fire under future climate conditions, we coupled the well established and comprehensively validated Dynamic Global Vegetation Model LPJmL5.0-FMS to the coupled climate model CM2Mc, which is based on the atmosphere model AM2 and the ocean model MOM5 (CM2Mc-LPJmL v1.0). In CM2Mc, we replaced the simple land surface model LaD with LPJmL and fully coupled the water and energy cycles. Exchanging LaD by LPJmL, and therefore switching from a static and prescribed vegetation to a dynamic vegetation, allows us to model important biosphere processes, including wildfire, tree mortality, permafrost, hydrological cycling, and the impacts of managed land (crop growth and irrigation).&lt;/p&gt;&lt;p&gt;With CM2Mc-LPJmL we conducted simulation experiments where atmospheric CO2 concentrations increased from a pre-industrial level up to 1280 ppm (impact phase) followed by a recovery phase where CO2 concentrations reach pre-industrial levels again. This experiment is performed with and without allowing for wildfires. We find a hysteresis of the biomass and vegetation cover in tropical forest systems, with a strong regional heterogeneity. After biomass loss along increasing atmospheric CO2 concentrations and accompanied mean surface temperature increase of about 4&amp;#176;C (impact phase), the system does not recover completely into its original state on its return path, even though atmospheric CO2 concentrations return to their original state. While not detecting large-scale tipping points, our results show a climate-induced hysteresis in tropical forest and lagged responses in forest recovery after the climate has returned to its original state. Wildfires slightly widen the climate-induced hysteresis in tropical forests and lead to a lagged response in forest recovery by ca. 30 years.&lt;/p&gt;

scholarly journals A missing link in the carbon cycle: phytoplankton light absorption

2021 ◽  
Author(s):  
Rémy Asselot ◽  
Frank Lunkeit ◽  
Philip Holden ◽  
Inga Hense
Keyword(s):  
Carbon Cycle ◽  
Co2 Emissions ◽  
Light Absorption ◽  
Climate System ◽  
Atmospheric Co2 ◽  
Co2 Concentrations ◽  
Recent Climate ◽  
Atmospheric Co2 Concentrations ◽  
The Impact

Abstract. Marine biota and biogeophysical mechanisms, such as phytoplankton light absorption, have attracted increasing attention in recent climate studies. Under global warming, the impact of phytoplankton on the climate system is expected to change. Previous studies analyzed the impact of phytoplankton light absorption under prescribed future atmospheric CO2 concentrations. However, the role of this biogeophysical mechanism under freely-evolving atmospheric CO2 concentration and future CO2 emissions remain unknown. To shed light on this research gap, we perform simulations with the EcoGEnIE Earth system model and prescribe CO2 emissions following the four Representative Concentration Pathways (RCP) scenarios. Under all the RCP scenario, our results indicate that phytopankton light absorption increases the surface chlorophyll biomass, the sea surface temperature, the atmospheric CO2 concentrations and the atmospheric temperature. Under the RCP2.6, RCP4.5 and RCP6.0 scenarios, the magnitude of changes due to phytoplankton light absorption are similar. However, under the RCP8.5 scenario, the changes in the climate system are less pronounced due to the temperature limitation of phytoplankton growth, highlighting the reduced effect of phytoplankton light absorption under strong warming. Additionally, this work evidences the major role of phytoplankton light absorption on the climate system, suggesting a highly uncertain feedbacks on the carbon cycle with uncertainties that are in the range of those known from the land biota.

scholarly journals Response of the carbon cycle in an intermediate complexity model to the different climate configurations of the last nine interglacials

2018 ◽  
Vol 14 (2) ◽  
pp. 239-253 ◽  
Author(s):  
Nathaelle Bouttes ◽  
Didier Swingedouw ◽  
Didier M. Roche ◽  
Maria F. Sanchez-Goni ◽  
Xavier Crosta
Keyword(s):  
Carbon Cycle ◽  
Ocean Circulation ◽  
Ice Sheet ◽  
Atmospheric Co2 ◽  
Oceanic Circulation ◽  
Intermediate Complexity ◽  
Co2 Concentrations ◽  
Co2 Levels ◽  
Atmospheric Co2 Concentrations ◽  
The Impact

Abstract. Atmospheric CO2 levels during interglacials prior to the Mid-Brunhes Event (MBE, ∼ 430 ka BP) were around 40 ppm lower than after the MBE. The reasons for this difference remain unclear. A recent hypothesis proposed that changes in oceanic circulation, in response to different external forcings before and after the MBE, might have increased the ocean carbon storage in pre-MBE interglacials, thus lowering atmospheric CO2. Nevertheless, no quantitative estimate of this hypothesis has been produced up to now. Here we use an intermediate complexity model including the carbon cycle to evaluate the response of the carbon reservoirs in the atmosphere, ocean and land in response to the changes of orbital forcings, ice sheet configurations and atmospheric CO2 concentrations over the last nine interglacials. We show that the ocean takes up more carbon during pre-MBE interglacials in agreement with data, but the impact on atmospheric CO2 is limited to a few parts per million. Terrestrial biosphere is simulated to be less developed in pre-MBE interglacials, which reduces the storage of carbon on land and increases atmospheric CO2. Accounting for different simulated ice sheet extents modifies the vegetation cover and temperature, and thus the carbon reservoir distribution. Overall, atmospheric CO2 levels are lower during these pre-MBE simulated interglacials including all these effects, but the magnitude is still far too small. These results suggest a possible misrepresentation of some key processes in the model, such as the magnitude of ocean circulation changes, or the lack of crucial mechanisms or internal feedbacks, such as those related to permafrost, to fully account for the lower atmospheric CO2 concentrations during pre-MBE interglacials.

scholarly journals Response of the carbon cycle to the different orbital configurations of the last 9 interglacials

2016 ◽  
Author(s):  
Nathaelle Bouttes ◽  
Didier Swingedouw ◽  
Didier Roche ◽  
Maria Sanchez-Goni ◽  
Xavier Crosta
Keyword(s):  
Carbon Cycle ◽  
Ocean Circulation ◽  
Ice Sheet ◽  
Atmospheric Co2 ◽  
Oceanic Circulation ◽  
Co2 Concentrations ◽  
Before And After ◽  
Ocean Carbon ◽  
Atmospheric Co2 Concentrations ◽  
The Impact

Abstract. Atmospheric CO2 levels during interglacials prior to the Mid Bruhnes Event (MBE, ~ 430 ka BP) have lower values of around 40 ppm than after the MBE. The reasons for this difference remain unclear. A recent hypothesis proposed that changes in oceanic circulation, in response to differences in external forcing before and after the MBE, might have increased the ocean carbon storage and thus explained the lower CO2. Nevertheless, no quantitative estimate of this hypothesis has been produced up to now. Here we use an intermediate complexity model including the carbon cycle to evaluate the response of the carbon reservoirs in the atmosphere, ocean and land in response to the changes of orbital forcings and atmospheric CO2 concentrations over the nine last interglacials. We show that the ocean takes up more carbon during pre-MBE interglacials in agreement with data, but the impact on atmospheric CO2 is limited to a few ppm. Terrestrial biosphere is simulated to be less developed in pre-MBE interglacials, which reduces the storage of carbon on land and increases atmospheric CO2. Accounting for different simulated ice sheet extents modifies the vegetation cover and temperature, and thus the carbon reservoir distribution. Overall, atmospheric CO2 is slightly smaller in these pre-MBE simulated interglacials including ice sheet variations, but the magnitude is still far too small. These results suggest a possible mis-representation of some key processes in the model, such as the magnitude of ocean circulation changes, or the lack of crucial mechanisms or internal feedbacks, such as those related to permafrost, that could explain the lower atmospheric CO2 concentrations during pre-MBE interglacials.

Defective intestinal amino acid absorption in Ace2 null mice

2012 ◽  
Vol 303 (6) ◽  
pp. G686-G695 ◽  
Author(s):  
Dustin Singer ◽  
Simone M. R. Camargo ◽  
Tamara Ramadan ◽  
Matthias Schäfer ◽  
Luca Mariotta ◽  
...  
Keyword(s):  
Amino Acids ◽  
Weight Gain ◽  
Amino Acid ◽  
Amino Acid Content ◽  
Wild Type ◽  
Neutral Amino Acids ◽  
Low Protein ◽  
Dependent Transport ◽  
Niacin Deficiency ◽  
The Impact

Mutations in the main intestinal and kidney luminal neutral amino acid transporter B0AT1 (Slc6a19) lead to Hartnup disorder, a condition that is characterized by neutral aminoaciduria and in some cases pellagra-like symptoms. These latter symptoms caused by low-niacin are thought to result from defective intestinal absorption of its precursor l-tryptophan. Since Ace2 is necessary for intestinal B0AT1 expression, we tested the impact of intestinal B0AT1 absence in ace2 null mice. Their weight gain following weaning was decreased, and Na+-dependent uptake of B0AT1 substrates measured in everted intestinal rings was defective. Additionally, high-affinity Na+-dependent transport of l-proline, presumably via SIT1 (Slc6a20), was absent, whereas glucose uptake via SGLT1 (Slc5a1) was not affected. Measurements of small intestine luminal amino acid content following gavage showed that more l-tryptophan than other B0AT1 substrates reach the ileum in wild-type mice, which is in line with its known lower apparent affinity. In ace2 null mice, the absorption defect was confirmed by a severalfold increase of l-tryptophan and of other neutral amino acids reaching the ileum lumen. Furthermore, plasma and muscle levels of glycine and l-tryptophan were significantly decreased in ace2 null mice, with other neutral amino acids displaying a similar trend. A low-protein/low-niacin diet challenge led to differential changes in plasma amino acid levels in both wild-type and ace2 null mice, but only in ace2 null mice to a stop in weight gain. Despite the combination of low-niacin with a low-protein diet, plasma niacin concentrations remained normal in ace2 null mice and no pellagra symptoms, such as photosensitive skin rash or ataxia, were observed. In summary, mice lacking Ace2-dependent intestinal amino acid transport display no total niacin deficiency nor clear pellagra symptoms, even under a low-protein and low-niacin diet, despite gross amino acid homeostasis alterations.

scholarly journals Heterogenous CO<sub>2</sub> and CH<sub>4</sub> content of glacial meltwater of the Greenland Ice Sheet and implications for subglacial carbon processes

2020 ◽  
Author(s):  
Andrea J. Pain ◽  
Jonathan B. Martin ◽  
Ellen E. Martin ◽  
Shaily Rahman
Keyword(s):  
Ice Sheet ◽  
Greenland Ice Sheet ◽  
Mineral Weathering ◽  
Atmospheric Co2 ◽  
The Arctic ◽  
Co2 Concentrations ◽  
Geochemical Models ◽  
Carbon Dioxide Co2 ◽  
Atmospheric Co2 Concentrations ◽  
The Impact

Abstract. Accelerated melting of the Greenland Ice Sheet (GrIS) has increased freshwater delivery to the Arctic Ocean and amplified the need to understand the impact of GrIS meltwater on Arctic greenhouse gas (GHG) budgets. We measured carbon dioxide (CO2) and methane (CH4) concentrations and δ13C values and use geochemical models to evaluate subglacial CH4 and CO2 sources and sinks in water discharging from three subglacial outlets of the GrIS in southwest (Isunnguata and Russell Glaciers) and southern Greenland (Kiattut Sermiat). CH4 concentrations vary by orders of magnitude between sites and are saturated with respect to atmospheric concentrations at Kiattut Sermiat, but are supersaturated at southwest sites, even though oxidation reduces concentrations by up to 50 % during periods of low discharge. CO2 concentrations range from supersaturated at Isunnguata to undersaturated at Kiattut Sermiat. CO2 is consumed by mineral weathering throughout the melt season at all sites, however differences in the magnitude of subglacial CO2 sources result in meltwaters that are either sources or sinks of atmospheric CO2. The predominant source of CO2 at Isunnguata is organic matter (OM) remineralization, but Russell and Kiattut Sermiat sites have multiple or heterogeneous subglacial CO2 sources that maintain atmospheric CO2 concentrations at Russell but not at Kiattut Sermiat where CO2 is undersaturated. These results highlight the variability in GHG dynamics under the GrIS. Constraining this variability will improve our understanding of the impact of GrIS melt on Arctic GHG budgets, as well as the role of continental ice sheets in GHG variations over glacial-interglacial timescales.

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