scholarly journals Analytical Methodology for a Metabolome Atlas of Goat’s Plasma, Milk and Feces Using 1H-NMR and UHPLC-HRMS

Metabolites ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 681
Author(s):  
Cécile Martias ◽  
Julie Gatien ◽  
Léa Roch ◽  
Nadine Baroukh ◽  
Sylvie Mavel ◽  
...  
Keyword(s):  
Metabolic Profile ◽  
Animal Health ◽  
Standard Operating Procedure ◽  
Single Type ◽  
Sample Type ◽  
Dairy Goats ◽  
Analytical Methodology ◽  
Dairy Animals ◽  
Sample Preparation Procedure

Metabolomics has been increasingly used in animal and food sciences. Animal health is one of the most important factor that can also alter animal integrity and welfare. Some studies have already investigated the link between health and metabolic profile of dairy animals. These studies in metabolomics often consider a single type of sample using a single analytical platform (nuclear magnetic resonance or mass spectrometry). Only few studies with multi-platform approaches are also used with a single or a multi type of sample, but they mainly consider dairy cows’ metabolome although dairy goats present similar diseases, that it could be interesting to detect early to preserve animal health and milk production. This study aims to create a metabolic atlas of goat plasma, milk and feces, based on healthy animals. Our study describes a standard operating procedure for three goat matrices: blood plasma, milk, and feces using multiple platforms (NMR (1H), UHPLC (RP)-MS and UHPLC (HILIC)-MS) that follows a unique sample preparation procedure for each sample type to be analyzed on multi-platforms basis. Our method was evaluated for its robustness and allowed a better characterization of goat metabolic profile in healthy conditions.

scholarly journals Optimization of Sample Preparation for Metabolomics Exploration of Urine, Feces, Blood and Saliva in Humans Using Combined NMR and UHPLC-HRMS Platforms

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4111
Author(s):  
Cécile Martias ◽  
Nadine Baroukh ◽  
Sylvie Mavel ◽  
Hélène Blasco ◽  
Antoine Lefèvre ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Clinical Studies ◽  
Standard Operating Procedure ◽  
Single Type ◽  
Sample Type ◽  
Metabolomics Data ◽  
Combining Data ◽  
Multiple Sample ◽  
Sample Preparation Procedure ◽  
Analytical Platforms

Currently, most clinical studies in metabolomics only consider a single type of sample such as urine, plasma, or feces and use a single analytical platform, either NMR or MS. Although some studies have already investigated metabolomics data from multiple fluids, the information is limited to a unique analytical platform. On the other hand, clinical studies investigating the human metabolome that combine multi-analytical platforms have focused on a single biofluid. Combining data from multiple sample types for one patient using a multimodal analytical approach (NMR and MS) should extend the metabolome coverage. Pre-analytical and analytical phases are time consuming. These steps need to be improved in order to move into clinical studies that deal with a large number of patient samples. Our study describes a standard operating procedure for biological specimens (urine, blood, saliva, and feces) using multiple platforms (1H-NMR, RP-UHPLC-MS, and HILIC-UHPLC-MS). Each sample type follows a unique sample preparation procedure for analysis on a multi-platform basis. Our method was evaluated for its robustness and was able to generate a representative metabolic map.

scholarly journals Characterization of Staphylococcus aureus Isolated from Chronically Infected Dairy Goats

2005 ◽  
Vol 88 (10) ◽  
pp. 3500-3509 ◽  
Author(s):  
P. Moroni ◽  
G. Pisoni ◽  
C. Vimercati ◽  
M. Rinaldi ◽  
B. Castiglioni ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Dairy Goats

scholarly journals Animal health constraints in dairy goats kept under smallholder farming systems in Kongwa and Mvomero Districts, Tanzania

2014 ◽  
Vol 6 (11) ◽  
pp. 268-279 ◽  
Author(s):  
Said Ngasa Shija Dismas ◽  
Jeremy Moses Kusiluka Lughano ◽  
Wilson Chenyambuga Sebastian ◽  
Shayo Deogratias ◽  
Paul Lekule Faustin
Keyword(s):  
Animal Health ◽  
Farming Systems ◽  
Dairy Goats ◽  
Smallholder Farming ◽  
Smallholder Farming Systems

scholarly journals AS ÁREAS RESIDENCIAIS DE CIRCULAÇÃO E DE REPRESENTAÇÃO DAS DOMUS DE BRACARA AUGUSTA

2013 ◽  
Author(s):  
Fernanda MAGALHÃES
Keyword(s):  
Domestic Architecture ◽  
Single Type ◽  
The Subject ◽  
Significant Data

<p>RESUMEN: En las últimas tres décadas han sido realizadas en Braga, dentro del «Proyecto de Salvamente de Bracara Augusta», una serie de trabajos de campos que han proporcionado abundante información arqueológica, lo que nos permitir analizar pormenorizadamente la arquitectura doméstica de la ciudad. Estos datos van a posibilitar el estudio y caracterización de algunos edificios y cuarterones de Bracara Augusta. A pesar de los numerosos restos de estructuras de habitación que han sido identificados en las distintas intervenciones, el presente estudio se limitará a examinar un único tipo de casa: la domus. En primer lugar, procuraremos contextualizar debidamente este tipo de casa. Seguidamente analizaremos las partes orgánicas asociadas a la representación y circulación, de tal forma que podamos caracterizar tanto sus formas y construcciones, como sus funciones.</p><p>ABSTRACT: In the last three decades were held in Braga within the «Projeto Salvamento de Bracara Augusta» a set of excavations, which have provided significant data allowing to analyze the subject of domestic architecture. This data will enable the study and characterization of some houses and quarters of Bracara Augusta. Although the wide archeological record related with private architecture this study is limited to the analysis of a single type of house, the domus. We intend to contextualize this type of house and to analyze its organic spaces associated to the representation and circulation in order to highlight their formal constructive and functional specificities.</p>

scholarly journals Characterization of the Intestinal Microbiota of Broiler Breeders With Different Egg Laying Rate

2020 ◽  
Vol 7 ◽  
Author(s):  
Zengqiao Yang ◽  
Chunhua Zhang ◽  
Jianping Wang ◽  
Pietro Celi ◽  
Xuemei Ding ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Feed Efficiency ◽  
Animal Health ◽  
Negative Relationship ◽  
Egg Laying ◽  
Ovary Cell ◽  
Feed Conversion ◽  
Broiler Breeders ◽  
Gastrointestinal Microbiota

The gastrointestinal microbiota plays a pivotal role in maintaining animal health, immunity and reproductive performances. However, literature about the relationship between microbiota and reproductive performance is limited. The aim of the present study was to determine differences in the intestinal microbiota of broiler breeders with different egg laying rate. A total of 200 AA+ parent broiler breeders (41-week-old) were separated into two groups according to their different egg laying rate [average egg laying rate group (AR: 78.57 ± 0.20%) and high egg laying rate group (HR: 90.79 ± 0.43%). Feed conversion ratio (FCR), ovary cell apoptosis rate (ApoCR) and relative abdominal fat weight were lower (p = 0.01), while the hatchability rate of qualified egg was higher (p = 0.04) in HR group than that in AR group. Phascolarctobacterium abundance were lower (p = 0.012) in ileum of HR birds. Romboutsia (genus) in ileum was negatively related to the feed efficiency (r = −0.58, p &lt; 0.05), Firmicutes (phylum) and Lactobacillus (genus) abundances in cecum were positively related to the egg laying rate (ELR) (r = 0.35 and 0.48, p &lt; 0.05), feed efficiency (r = 0.42 and 0.43, p &lt; 0.05), while Spirochaetes (phylum) and Sphaerochaeta (genus) abundances in cecum were negatively related to the ELR (r = −0.43 and −0.70, p &lt; 0.05), feed efficiency (r = 0.54 and 0.48, p &lt; 0.05), and positively related to ApoCR (r = 0.46 and 0.47, p &lt; 0.05). Our results suggested that microbiota, such as Firmicutes (phylum) and Lactobacillus (genus) have positive relationship, while Spirochaetes (phylum) and Romboutsia (genus) abundances exert negative relationship with broiler breeders' reproductive performances.

scholarly journals Analytical Dissection of an Automotive Li-Ion Pouch Cell

Batteries ◽  
2019 ◽  
Vol 5 (4) ◽  
pp. 67 ◽  
Author(s):  
Kovachev ◽  
Schröttner ◽  
Gstrein ◽  
Aiello ◽  
Hanzu ◽  
...  
Keyword(s):  
State Of The Art ◽  
Measured Data ◽  
Li Ion Batteries ◽  
Complete Sample ◽  
Analytical Methodology ◽  
Preparation Methods ◽  
Analysis Techniques ◽  
Sample Preparation Procedure ◽  
Li Ion ◽  
Further Development

Information derived from microscopic images of Li-ion cells is the base for research on the function, the safety, and the degradation of Li-ion batteries. This research was carried out to acquire information required to understand the mechanical properties of Li-ion cells. Parameters such as layer thicknesses, material compositions, and surface properties play important roles in the analysis and the further development of Li-ion batteries. In this work, relevant parameters were derived using microscopic imaging and analysis techniques. The quality and the usability of the measured data, however, are tightly connected to the sample generation, the preparation methods used, and the measurement device selected. Differences in specimen post-processing methods and measurement setups contribute to variability in the measured results. In this paper, the complete sample preparation procedure and analytical methodology are described, variations in the measured dataset are highlighted, and the study findings are discussed in detail. The presented results were obtained from an analysis conducted on a state-of-the-art Li-ion pouch cell applied in an electric vehicle that is currently commercially available.

ChemInform Abstract: In vitro Microbiological Characterization of Novel Cyclic Homopentapeptides, CP-101,680 and CP-163,234, for Animal Health Use.

ChemInform ◽  
2010 ◽  
Vol 31 (14) ◽  
pp. no-no
Author(s):  
Laura J. L. Norcia ◽  
Annette M. Silvia ◽  
John P. Dirlam ◽  
Rodney C. Schnur ◽  
Jay M. Bergeron ◽  
...  
Keyword(s):  
Animal Health
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