Integrated Microfluidic-Based Platforms for On-Site Detection and Quantification of Infectious Pathogens: Towards On-Site Medical Translation of SARS-CoV-2 Diagnostic Platforms

The rapid detection and quantification of infectious pathogens is an essential component to the control of potentially lethal outbreaks among human populations worldwide. Several of these highly infectious pathogens, such as Middle East respiratory syndrome (MERS) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), have been cemented in human history as causing epidemics or pandemics due to their lethality and contagiousness. SARS-CoV-2 is an example of these highly infectious pathogens that have recently become one of the leading causes of globally reported deaths, creating one of the worst economic downturns and health crises in the last century. As a result, the necessity for highly accurate and increasingly rapid on-site diagnostic platforms for highly infectious pathogens, such as SARS-CoV-2, has grown dramatically over the last two years. Current conventional non-microfluidic diagnostic techniques have limitations in their effectiveness as on-site devices due to their large turnaround times, operational costs and the need for laboratory equipment. In this review, we first present criteria, both novel and previously determined, as a foundation for the development of effective and viable on-site microfluidic diagnostic platforms for several notable pathogens, including SARS-CoV-2. This list of criteria includes standards that were set out by the WHO, as well as our own “seven pillars” for effective microfluidic integration. We then evaluate the use of microfluidic integration to improve upon currently, and previously, existing platforms for the detection of infectious pathogens. Finally, we discuss a stage-wise means to translate our findings into a fundamental framework towards the development of more effective on-site SARS-CoV-2 microfluidic-integrated platforms that may facilitate future pandemic diagnostic and research endeavors. Through microfluidic integration, many limitations in currently existing infectious pathogen diagnostic platforms can be eliminated or improved upon.

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Rapid detection and quantification of adulteration in Chinese hawthorn fruits powder by near-infrared spectroscopy combined with chemometrics

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy ◽

10.1016/j.saa.2020.119346 ◽

2021 ◽

Vol 250 ◽

pp. 119346

Author(s):

Xuefen Sun ◽

Huiling Li ◽

Yuan Yi ◽

Haimin Hua ◽

Ying Guan ◽

...

Keyword(s):

Infrared Spectroscopy ◽

Near Infrared Spectroscopy ◽

Rapid Detection ◽

Near Infrared ◽

Detection And Quantification

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Development of a rapid detection and quantification method of Karenia mikimotoi by real-time quantitative PCR

Harmful Algae ◽

10.1016/j.hal.2012.03.004 ◽

2012 ◽

Vol 17 ◽

pp. 83-91 ◽

Cited By ~ 41

Author(s):

Jian Yuan ◽

Tiezhu Mi ◽

Yu Zhen ◽

Zhigang Yu

Keyword(s):

Real Time ◽

Quantitative Pcr ◽

Rapid Detection ◽

Karenia Mikimotoi ◽

Real Time Quantitative Pcr ◽

Quantification Method ◽

Detection And Quantification

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Advanced test protocols for rapid detection and quantification of nonlinear dynamic responses in aeroengine casing assemblies

Nonlinear Dynamics ◽

10.1007/s11071-021-06375-6 ◽

2021 ◽

Author(s):

Seyed-Ehsan Mir-Haidari ◽

Kamran Behdinan

Keyword(s):

Nonlinear Dynamic ◽

Rapid Detection ◽

Dynamic Responses ◽

Detection And Quantification

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Detection of Peanut Allergen Traces with a Real Time PCR Assay - The Challenge to Protect Food-Allergic Consumers

Journal of Food Research ◽

10.5539/jfr.v7n1p32 ◽

2017 ◽

Vol 7 (1) ◽

pp. 32 ◽

Cited By ~ 2

Author(s):

Dimitra Houhoula ◽

Stamatios Koussissis ◽

Vladimiros Lougovois ◽

John Tsaknis ◽

Dimitra Kassavita ◽

...

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Rapid Detection ◽

Food Products ◽

Molecular Techniques ◽

Food Allergens ◽

Pcr Assay ◽

Peanut Allergen ◽

Pcr Method ◽

Detection And Quantification

The aim of the present study was the implementation of molecular techniques in the detection and quantification of allergic substances of peanut in various kinds of food products, e.g., breakfast cereals, chocolates and biscuits that are frequently related to allergies. In some cases, the presence of peanuts can be due to contamination during production and are not declared on the label. A total of 152 samples were collected from supermarkets and were analysed by a Real Time PCR method. The results indicated that 125 samples (83,3%) were found positive in peanut traces but the most important finding is that from the 84 samples that had no allergen declaration for peanuts, 48 (57,1%) of them were found positive. In conclusion, Real Time PCR can be a very important tool for the rapid detection and quantification of food allergens.

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The Paradoxes of Racism

Government and Opposition ◽

10.1111/j.1477-7053.1993.tb01385.x ◽

1993 ◽

Vol 28 (4) ◽

pp. 512-525 ◽

Cited By ~ 2

Author(s):

John Dunn

Keyword(s):

Human Populations ◽

Human History

IN ITS MOST ELABORATELY ARTICULATED FORM RACISM IS AN array of theoretical views about the epistemic clarity and human import of the practice of individuating human populations in terms of their presumed biological descent. In this form the term can refer with some precision, and largely independently of context, to a quite specific body of beliefs. In far more diffuse and far less theoretical forms, however, and in forms which are also far more widely distributed in human history, it can and does refer just as readily to the highlighting of distinctions which are certainly at least as much cultural as they are genetic, and which are inextricably located in a context of more or less intimate and painful confrontation between human groups. In this second form there cannot in principle be anything comparably determinate for the term racism to refer to.

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