Enhanced growth and lipid production of microalgae under mixotrophic culture condition: Effect of light intensity, glucose concentration and fed-batch cultivation

2012 ◽  
Vol 110 ◽  
pp. 510-516 ◽  
Author(s):  
Benjamas Cheirsilp ◽  
Salwa Torpee
Keyword(s):  
Light Intensity ◽  
Culture Condition ◽  
Glucose Concentration ◽  
Lipid Production ◽  
Batch Cultivation ◽  
Fed Batch ◽  
Mixotrophic Culture ◽  
Condition Effect ◽  
Fed Batch Cultivation ◽  
Effect Of Light

scholarly journals Feeding Strategies of Two-Stage Fed-Batch Cultivation Processes for Microbial Lipid Production from Sugarcane Top Hydrolysate and Crude Glycerol by the Oleaginous Red Yeast Rhodosporidiobolus fluvialis

2020 ◽  
Vol 8 (2) ◽  
pp. 151 ◽  
Author(s):  
Poontawee ◽  
Limtong
Keyword(s):  
Crude Glycerol ◽  
Lipid Production ◽  
Cell Mass ◽  
Batch Cultivation ◽  
Feeding Strategies ◽  
Fed Batch ◽  
Two Stage ◽  
Microbial Lipid Production ◽  
Constant Feeding ◽  
Fed Batch Cultivation

Microbial lipids are able to produce from various raw materials including lignocellulosic biomass by the effective oleaginous microorganisms using different cultivation processes. This study aimed to enhance microbial lipid production from the low-cost substrates namely sugarcane top hydrolysate and crude glycerol by Rhodosporidiobolus fluvialis DMKU-SP314, using two-stage fed-batch cultivation with different feeding strategies in a 3 L stirred-tank fermenter. The effect of two feeding strategies of 147.5 g/L crude glycerol solution was evaluated including pulse feeding at different starting time points (48, 24, and 72 h after initiation of batch operation) and constant feeding at different dilution rates (0.012, 0.020, and 0.033 h−1). The maximum lipid concentration of 23.6 g/L and cell mass of 38.5 g/L were achieved when constant feeding was performed at the dilution rate of 0.012 h−1 after 48 h of batch operation, which represented 1.24-fold and 1.27-fold improvements in the lipid and cell mass concentration, respectively. Whereas, batch cultivation provided 19.1 g/L of lipids and 30.3 g/L of cell mass. The overall lipid productivity increased to 98.4 mg/L/d in the two-stage fed-batch cultivation. This demonstrated that the two-stage fed-batch cultivation with constant feeding strategy has the possibility to apply for large-scale production of lipids by yeast.

Lipid production from hemicellulose withLipomyces starkeyiin a pH regulated fed-batch cultivation

Yeast ◽  
2016 ◽  
Vol 33 (8) ◽  
pp. 451-462 ◽  
Author(s):  
Jule Brandenburg ◽  
Johanna Blomqvist ◽  
Jana Pickova ◽  
Nemailla Bonturi ◽  
Mats Sandgren ◽  
...  
Keyword(s):  
Lipid Production ◽  
Batch Cultivation ◽  
Fed Batch ◽  
Fed Batch Cultivation

Effects of photobioreactor configuration, nitrogen source and light intensity on the fed-batch cultivation of Arthrospira (Spirulina) platensis. Bioenergetic aspects

2012 ◽  
Vol 37 ◽  
pp. 309-317 ◽  
Author(s):  
Raquel Pedrosa Bezerra ◽  
Marcelo Chuei Matsudo ◽  
Sunao Sato ◽  
Patrizia Perego ◽  
Attilio Converti ◽  
...  
Keyword(s):  
Light Intensity ◽  
Nitrogen Source ◽  
Spirulina Platensis ◽  
Batch Cultivation ◽  
Fed Batch ◽  
Fed Batch Cultivation

scholarly journals Optimization of Synthetic Media Composition for Kluyveromyces marxianus Fed-Batch Cultivation

Fermentation ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 62
Author(s):  
Konstantins Dubencovs ◽  
Janis Liepins ◽  
Arturs Suleiko ◽  
Anastasija Suleiko ◽  
Reinis Vangravs ◽  
...  
Keyword(s):  
Kluyveromyces Marxianus ◽  
Reaction Medium ◽  
Value Added ◽  
Batch Cultivation ◽  
Batch Processes ◽  
Biomass Growth ◽  
High Cell ◽  
Media Composition ◽  
Fed Batch ◽  
Fed Batch Cultivation

The Kluyveromyces marxianus yeast recently has gained considerable attention due to its applicability in high-value-added product manufacturing. In order to intensify the biosynthesis rate of a target product, reaching high biomass concentrations in the reaction medium is mandatory. Fed-batch processes are an attractive and efficient way how to achieve high cell densities. However, depending on the physiology of the particular microbial strain, an optimal media composition should be used to avoid by-product synthesis and, subsequently, a decrease in overall process effi-ciency. Thus, the aim of the present study was to optimise the synthetic growth medium and feeding solution compositions (in terms of carbon, nitrogen, phosphorous, magnesium, and calcium concentrations) for high cell density K. marxianus fed‑batch cultivations. Additionally, the biomass yields from the vitamin mixture and other macro/microelements were identified. A model predictive control algorithm was successfully applied for a fed-batch cultivation control. Biomass growth and substrate consumption kinetics were compared with the mathematical model predictions. Finally, 2‑phenylethanol biosynthesis was induced and its productivity was estimated. The determined optimal macronutrient ratio for K. marxianus biomass growth was identified as C:N:P = 1:0.07:0.011. The maximal attained yeast biomass concentration was close to 70 g·L-1 and the 2-PE biosynthesis rate was 0.372 g·L−1·h−1, with a yield of 74% from 2-phenylalanine.

A feedforward–feedback substrate controller based on a Kalman filter for a fed-batch cultivation of Escherichia coli producing phytase

2005 ◽  
Vol 29 (5) ◽  
pp. 1113-1120 ◽  
Author(s):  
M. Arndt ◽  
S. Kleist ◽  
G. Miksch ◽  
K. Friehs ◽  
E. Flaschel ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Kalman Filter ◽  
Batch Cultivation ◽  
Fed Batch ◽  
Fed Batch Cultivation

scholarly journals Fed-Batch Cultivation of Saccharomyces cerevisiae in a Hyperbaric Bioreactor

2003 ◽  
Vol 19 (2) ◽  
pp. 665-671 ◽  
Author(s):  
I. Belo ◽  
R. Pinheiro ◽  
M. Mota
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Batch Cultivation ◽  
Fed Batch ◽  
Fed Batch Cultivation

Fed-batch cultivation of Desmodesmus sp. in anaerobic digestion wastewater for improved nutrient removal and biodiesel production

2015 ◽  
Vol 184 ◽  
pp. 116-122 ◽  
Author(s):  
Fang Ji ◽  
Yuguang Zhou ◽  
Aiping Pang ◽  
Li Ning ◽  
Kibet Rodgers ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Nutrient Removal ◽  
Batch Cultivation ◽  
Biodiesel Production ◽  
Fed Batch ◽  
Fed Batch Cultivation
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