Yeast Associated with Rice Phylloplane and Their Contribution to Control of Rice Sheath Blight Disease

The phylloplane is an important habitat for yeasts and these yeasts may have antagonistic activities against pathogens and could be used as biocontrol agents. To investigate rice phylloplane yeasts, 282 strains were isolated from 89 rice leaf samples and identified as 15 known yeast species in the phylum Ascomycota and 35 known and two potential new species in the phylum Basidiomycota. The majority of rice phylloplane yeasts belonged to the phylum Basidiomycota. The evaluation of antagonistic activities of 83 yeast strains against rice pathogenic fungi Pyricularia oryzae, Rhizoctonia solani, Fusarium moniliforme, Helminthosporium oryzae and Curvularia lunata revealed that 14 strains inhibited these pathogens. Among the antagonistic strains, Torulaspora indica DMKU-RP31, T. indica DMKU-RP35 and Wickerhamomyces anomalus DMKU-RP25 inhibited all rice pathogens tested, and the production of volatile organic compounds, fungal cell wall degrading enzymes and biofilm were the possible antagonistic mechanisms against all rice pathogens tested in vitro. These yeast strains were evaluated for controlling rice sheath blight caused by R. solani in rice plants in the greenhouse and were found to suppress the disease by 60.0–70.3%, whereas 3% validamycin suppressed by 83.8%. Therefore, they have potential for being developed to be used as biocontrol agents for rice sheath blight.

Download Full-text

Phylloplane Yeasts as Potential Biocontrol Agents for Rice Sheath Blight Disease

Major Fungal Diseases of Rice ◽

10.1007/978-94-017-2157-8_17 ◽

2001 ◽

pp. 235-252 ◽

Cited By ~ 1

Author(s):

A. K. M. Shahjahan ◽

M. C. Rush ◽

D. E. Groth

Keyword(s):

Sheath Blight ◽

Biocontrol Agents ◽

Rice Sheath Blight ◽

Sheath Blight Disease ◽

Blight Disease ◽

Phylloplane Yeasts

Download Full-text

Evolution from Natural β-Carboline Alkaloids to Obtain 1,2,4,9-tetrahydro-3-thia-9-aza-fluorene Derivatives as Potent Fungicidal Agents against Rhizoctonia solani

International Journal of Molecular Sciences ◽

10.3390/ijms19124044 ◽

2018 ◽

Vol 19 (12) ◽

pp. 4044 ◽

Cited By ~ 1

Author(s):

Junmin Xi ◽

Zhijun Zhang ◽

Qi Zhu ◽

Guohua Zhong

Keyword(s):

Rhizoctonia Solani ◽

Fungicidal Activity ◽

Sheath Blight ◽

Potential Candidate ◽

Oxygen Species ◽

Rice Sheath Blight ◽

Validamycin A ◽

Rice Disease

Rice sheath blight, caused by Rhizoctonia solani, is a globally important rice disease and the increasing resistance of this pathogen highlights the need for new active compounds against rice sheath blight. In this study, natural β-carboline alkaloids were optimized to obtain a series of 1,2,4,9-tetrahydro-3-thia-9-aza-fluorene derivatives and evaluated for their fungicidal activity and mode of action against R. solani. Of these compounds, 18 exhibited significant in vitro fungicidal activity against R. solani, with an EC50 value of 2.35 μg/mL, and was more active than validamycin A. In vivo bioassay also demonstrated that 18 displayed superior protective and curative activities as compared to validamycin A. Mechanistically, 18 not only induced the loss of mitochondrial membrane potential and accumulation of reactive oxygen species, but also interfered with DNA synthesis. Therefore, compound 18 displayed pronounced in vitro and in vivo fungicidal activity against R. solani and could be used as a potential candidate for the control of rice sheath blight.

Download Full-text

In vitro and in vivo antifungal properties of silver nanoparticles against Rhizoctonia solani , a common agent of rice sheath blight disease

IET Nanobiotechnology ◽

10.1049/iet-nbt.2015.0121 ◽

2016 ◽

Vol 11 (3) ◽

pp. 236-240 ◽

Cited By ~ 30

Author(s):

Meysam Soltani Nejad ◽

Gholam Hosein Shahidi Bonjar ◽

Mehrdad Khatami ◽

Abbas Amini ◽

Sonia Aghighi

Keyword(s):

Silver Nanoparticles ◽

Rhizoctonia Solani ◽

Sheath Blight ◽

Rice Sheath Blight ◽

Sheath Blight Disease ◽

Antifungal Properties ◽

Blight Disease

Download Full-text

Combinatorial efficacy of Trichoderma spp. and Pseudomonas fluorescens to enhance suppression of cell wall degrading enzymes produced by Fusarium wilt of Arachis hypogaea.L

International Journal of Agricultural Research Innovation and Technology ◽

10.3329/ijarit.v7i2.35320 ◽

2018 ◽

Vol 7 (2) ◽

pp. 36-42 ◽

Cited By ~ 2

Author(s):

P Rajeswari ◽

R Kapoor

Keyword(s):

Fusarium Oxysporum ◽

Pseudomonas Fluorescens ◽

Trichoderma Harzianum ◽

Trichoderma Viride ◽

Pectin Methylesterase ◽

Biocontrol Agents ◽

Cell Wall Degrading Enzymes ◽

Trichoderma Spp ◽

Pectinolytic Enzymes

Fusarium oxysporum, the soil borne pathogen causes vascular wilt, on majority of crop plants. It has been demonstrated that two different species of Trichoderma and Pseudomonas fluorescens suppress disease by different mechanisms. Therefore, application of a mixture of these biocontrol agents, and thus of several suppressive mechanisms, may represent a viable control strategy. A necessity for biocontrol by combinations of biocontrol agents can be the compatibility of the co-inoculated micro-organisms. Hence, compatibility between Trichoderma spp. and Pseudomonas fluorescens that have the ability to suppress Fusarium oxysporum in vitro on the activity of pectinolytic enzymes of Fusarium oxysporum. The activity of pectinolytic enzymes, i.e. pectin methyl esterase, endo and exo polymethylgalacturonases and exo and endo pectin trans eliminases produced by Fusarium oxysporum (Control) was higher. Maximum inhibition of pectin methylesterase, exo and endo polymethylgalacturonase and exo and endopectin trans eliminase was shown by culture filtrate of Trichoderma viride + Pseudomonas fluorescens (Tv+Pf) (1+2%), followed by Trichoderma harzianum + Pseudomonas fluorescens, (Th +Pf) (1.5+2%) and Trichoderma viride + Trichoderma harzianum (Tv+Th) (1+1.5%). However, pathogenecity suppression of Fusarium oxysporum, a causative of Arachis hypogaea. L by the compatible combination of Trichodema viride + Pseudomonas fluorescens (1+2%) was significantly better as compared to the single bio-agent. This indicates that specific interactions between biocontrol agents influence suppression of pathogenicity factors directly by combinations of these compatible bio-agents.Int. J. Agril. Res. Innov. & Tech. 7 (2): 36-42, December, 2017

Download Full-text

In vitro evaluation of bio agents against rice sheath blight pathogen Rhizoctonia solani Kuhn

INTERNATIONAL JOURNAL OF PLANT PROTECTION ◽

10.15740/has/ijpp/11.2/146-150 ◽

2018 ◽

Vol 11 (2) ◽

pp. 146-150

Author(s):

Shrishti Lingwal ◽

Vishwanath

Keyword(s):

Rhizoctonia Solani ◽

Sheath Blight ◽

Rice Sheath Blight ◽

In Vitro Evaluation

Download Full-text

Biocontrol of Rice Seedling Rot Disease Caused by Curvularia lunata and Helminthosporium oryzae by Epiphytic Yeasts from Plant Leaves

Microorganisms ◽

10.3390/microorganisms8050647 ◽

2020 ◽

Vol 8 (5) ◽

pp. 647

Author(s):

Savitree Limtong ◽

Parichat Into ◽

Panchapond Attarat

Keyword(s):

Fungal Pathogens ◽

Rice Seedling ◽

Significant Loss ◽

Curvularia Lunata ◽

Yeast Strains ◽

Seedling Rot ◽

Rot Disease ◽

Epiphytic Yeast ◽

Helminthosporium Oryzae

Seedling rot disease in rice leads to significant loss in the production of seedlings. This research was conducted to explore yeasts that could be used as biological control agents against rice seedling rot disease caused by Curvularia lunata and Helminthosporium oryzae. In total, 167 epiphytic yeast strains were evaluated, revealing that 13 of these yeast strains demonstrated antagonistic activities against fungal pathogens and either C. lunata DOAC 2313 or H. oryzae DOAC 2293. The volatile organic compounds (VOCs) and biofilm produced were possible antagonistic mechanisms in vitro for all the antagonistic yeast strains. Using nursery trays in a greenhouse, this study evaluated the control of rice seedling rot disease caused by these two fungal pathogens using antagonistic yeasts, identified in the present study and from our previous study. Torulaspora indica DMKU-RP31 and Wickerhamomyces anomalus YE-42 were found to completely control rice seedling rot disease caused by both of these fungal pathogens. Furthermore, W. anomalus DMKU-RP04 revealed 100% disease control when the disease was caused by H. oryzae. This is the first report on using antagonistic yeasts to control rice seedling rot disease caused by C. lunata and H. oryzae. These three antagonistic yeasts also showed promising potential for development as biocontrol agents against rice seedling rot disease caused by fungi.

Download Full-text