scholarly journals Microbial Diversity of Psychrotolerant Bacteria Isolated from Wild Flora of Andes Mountains and Patagonia of Chile towards the Selection of Plant Growth-Promoting Bacterial Consortia to Alleviate Cold Stress in Plants

2021 ◽  
Vol 9 (3) ◽  
pp. 538 ◽  
Author(s):  
Paulina Vega-Celedón ◽  
Guillermo Bravo ◽  
Alexis Velásquez ◽  
Fernanda P. Cid ◽  
Miryam Valenzuela ◽  
...  
Keyword(s):  
Plant Growth ◽  
Cold Stress ◽  
Acc Deaminase ◽  
Andes Mountains ◽  
Wild Plants ◽  
Rrna Gene ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Consortia ◽  
Plant Growth Promoting ◽  
Growth Promoting

Cold stress decreases the growth and productivity of agricultural crops. Psychrotolerant plant growth-promoting bacteria (PGPB) may protect and promote plant growth at low temperatures. The aims of this study were to isolate and characterize psychrotolerant PGPB from wild flora of Andes Mountains and Patagonia of Chile and to formulate PGPB consortia. Psychrotolerant strains were isolated from 11 wild plants (rhizosphere and phyllosphere) during winter of 2015. For the first time, bacteria associated with Calycera, Orites, and Chusquea plant genera were reported. More than 50% of the 130 isolates showed ≥33% bacterial cell survival at temperatures below zero. Seventy strains of Pseudomonas, Curtobacterium, Janthinobacterium, Stenotrophomonas, Serratia, Brevundimonas, Xanthomonas, Frondihabitans, Arthrobacter, Pseudarthrobacter, Paenarthrobacter, Brachybacterium, Clavibacter, Sporosarcina, Bacillus, Solibacillus, Flavobacterium, and Pedobacter genera were identified by 16S rRNA gene sequence analyses. Ten strains were selected based on psychrotolerance, auxin production, phosphate solubilization, presence of nifH (nitrogenase reductase) and acdS (1-aminocyclopropane-1-carboxylate (ACC) deaminase) genes, and anti-phytopathogenic activities. Two of the three bacterial consortia formulated promoted tomato plant growth under normal and cold stress conditions. The bacterial consortium composed of Pseudomonas sp. TmR5a & Curtobacterium sp. BmP22c that possesses ACC deaminase and ice recrystallization inhibition activities is a promising candidate for future cold stress studies.

scholarly journals Effect of Pyroligneous Acid on the Microbial Community Composition and Plant Growth-Promoting Bacteria (PGPB) in Soils

Soil Systems ◽  
2022 ◽  
Vol 6 (1) ◽  
pp. 10
Author(s):  
Anithadevi Kenday Sivaram ◽  
Logeshwaran Panneerselvan ◽  
Kannappar Mukunthan ◽  
Mallavarapu Megharaj
Keyword(s):  
Microbial Community ◽  
Plant Growth ◽  
Microbial Diversity ◽  
Growth And Yield ◽  
Rrna Gene ◽  
Plant Growth Promoting Bacteria ◽  
Microbial Composition ◽  
Pyroligneous Acid ◽  
Plant Growth Promoting ◽  
Growth Promoting

Pyroligneous acid (PA) is often used in agriculture as a plant growth and yield enhancer. However, the influence of PA application on soil microorganisms is not often studied. Therefore, in this study, we investigated the effect of PA (0.01–5% w/w in soil) on the microbial diversity in two different soils. At the end of eight weeks of incubation, soil microbial community dynamics were determined by Illumina-MiSeq sequencing of 16S rRNA gene amplicons. The microbial composition differed between the lower (0.01% and 0.1%) and the higher (1% and 5%) concentration in both PA spiked soils. The lower concentration of PA resulted in higher microbial diversity and dehydrogenase activity (DHA) compared to the un-spiked control and the soil spiked with high PA concentrations. Interestingly, PA-induced plant growth-promoting bacterial (PGPB) genera include Bradyrhizobium, Azospirillum, Pseudomonas, Mesorhizobium, Rhizobium, Herbaspiriluum, Acetobacter, Beijerinckia, and Nitrosomonas at lower concentrations. Additionally, the PICRUSt functional analysis revealed the predominance of metabolism as the functional module’s primary component in both soils spiked with 0.01% and 0.1% PA. Overall, the results elucidated that PA application in soil at lower concentrations promoted soil DHA and microbial enrichment, particularly the PGPB genera, and thus have great implications for improving soil health.

scholarly journals Alone Yet Not Alone: Frankia Lives Under the Same Roof With Other Bacteria in Actinorhizal Nodules

2021 ◽  
Vol 12 ◽  
Author(s):  
Faten Ghodhbane-Gtari ◽  
Timothy D’Angelo ◽  
Abdellatif Gueddou ◽  
Sabrine Ghazouani ◽  
Maher Gtari ◽  
...  
Keyword(s):  
16S Rrna ◽  
Plant Growth ◽  
16S Rrna Gene ◽  
Root Nodules ◽  
Actinorhizal Plants ◽  
Rrna Gene ◽  
Plant Growth Promoting Bacteria ◽  
Metagenomic Sequencing ◽  
Plant Growth Promoting ◽  
Growth Promoting

Actinorhizal plants host mutualistic symbionts of the nitrogen-fixing actinobacterial genus Frankia within nodule structures formed on their roots. Several plant-growth-promoting bacteria have also been isolated from actinorhizal root nodules, but little is known about them. We were interested investigating the in planta microbial community composition of actinorhizal root nodules using culture-independent techniques. To address this knowledge gap, 16S rRNA gene amplicon and shotgun metagenomic sequencing was performed on DNA from the nodules of Casuarina glauca. DNA was extracted from C. glauca nodules collected in three different sampling sites in Tunisia, along a gradient of aridity ranging from humid to arid. Sequencing libraries were prepared using Illumina NextEra technology and the Illumina HiSeq 2500 platform. Genome bins extracted from the metagenome were taxonomically and functionally profiled. Community structure based off preliminary 16S rRNA gene amplicon data was analyzed via the QIIME pipeline. Reconstructed genomes were comprised of members of Frankia, Micromonospora, Bacillus, Paenibacillus, Phyllobacterium, and Afipia. Frankia dominated the nodule community at the humid sampling site, while the absolute and relative prevalence of Frankia decreased at the semi-arid and arid sampling locations. Actinorhizal plants harbor similar non-Frankia plant-growth-promoting-bacteria as legumes and other plants. The data suggests that the prevalence of Frankia in the nodule community is influenced by environmental factors, with being less abundant under more arid environments.

ACC deaminase from plant growth-promoting bacteria affects crown gall development

2007 ◽  
Vol 53 (12) ◽  
pp. 1291-1299 ◽  
Author(s):  
Youai Hao ◽  
Trevor C. Charles ◽  
Bernard R. Glick
Keyword(s):  
Plant Growth ◽  
Castor Bean ◽  
Crown Gall ◽  
Acc Deaminase ◽  
Plant Growth Promoting Bacteria ◽  
Bean Plants ◽  
Tumour Formation ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Agrobacterium Tumefaciens C58

In addition to the well-known roles of indoleacetic acid and cytokinin in crown gall formation, the plant hormone ethylene also plays an important role in this process. Many plant growth-promoting bacteria (PGPB) encode the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase, which can degrade ACC, the immediate precursor of ethylene in plants, to α-ketobutyrate and ammonia and thereby lower plant ethylene levels. To study the effect of ACC deaminase on crown gall development, an ACC deaminase gene from the PGPB Pseudomonas putida UW4 was introduced into Agrobacterium tumefaciens C58, so that the effect of ACC deaminase activity on tumour formation in tomato and castor bean plants could be assessed. Plants were also coinoculated with A. tumefaciens C58 and P. putida UW4 or P. putida UW4-acdS– (an ACC deaminase minus mutant strain). In both types of experiments, it was observed that the presence of ACC deaminase generally inhibited tumour development on both tomato and castor bean plants.

Levels of ACC and related compounds in exudate and extracts of canola seeds treated with ACC deaminase-containing plant growth-promoting bacteria

2001 ◽  
Vol 47 (4) ◽  
pp. 368-372 ◽  
Author(s):  
Donna M Penrose ◽  
Bernard R Glick
Keyword(s):  
Plant Growth ◽  
Acc Deaminase ◽  
Aminobutyric Acid ◽  
Plant Growth Promoting Bacteria ◽  
Canola Seed ◽  
Test Part ◽  
Seed Exudate ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Related Compounds

It was previously proposed that plant growth-promoting bacteria that possess 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase could utilize ACC that is present in the exudate of germinating canola seeds. The uptake and cleavage of ACC by these bacteria would lower the level of ACC, and thus ethylene within the plant, and reduce the extent of its inhibition on root elongation. To test part of the above mentioned model, ACC levels were monitored in canola seed tissues and exudate during germination. Lower amounts of ACC were present in the exudate and tissues of seeds treated with the plant growth-promoting bacterium Enterobacter cloacae CAL3, than in control seeds treated with MgSO4. The ACC-related compounds, α- and γ-aminobutyric acids, both known to stimulate ethylene production, were also measured in the canola seed exudate and tissues. Approximately the same levels of α-aminobutyric acid were present in the exudates of the bacterium-treated seeds and the control seeds, but the amount of α-aminobutyric acid was lower in the tissues of the bacterium-treated seeds than in the control seeds. Smaller quantities of γ-aminobutyric acid were seen in both the exudate and tissues of the E. cloacae CAL3-treated seeds than in the control seeds.Key words: ACC ethylene, canola, seed extract, seed exudate, plant growth-promoting bacteria.

Involvement ofgacSandrpoSin enhancement of the plant growth-promoting capabilities ofEnterobacter cloacaeCAL2 and UW4

2001 ◽  
Vol 47 (8) ◽  
pp. 698-705 ◽  
Author(s):  
Saleema S Saleh ◽  
Bernard R Glick
Keyword(s):  
Plant Growth ◽  
Pseudomonas Fluorescens ◽  
Indoleacetic Acid ◽  
Acc Deaminase ◽  
Enterobacter Cloacae ◽  
Lag Phase ◽  
Plant Growth Promoting Bacteria ◽  
Multicopy Plasmid ◽  
Plant Growth Promoting ◽  
Growth Promoting

The plant growth-promoting bacteria Enterobacter cloacae CAL2 and UW4 were genetically transformed with a multicopy plasmid containing an rpoS or gacS gene from Pseudomonas fluorescens. The transformed strains were compared with the nontransformed strains for growth, indoleacetic acid (IAA) production, antibiotic production, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, siderophore production, cell morphology, and the ability to promote canola root elongation. All transformed strains had a longer lag phase, were slower in reaching stationary phase, and attained a higher cell density than the nontransformed strains. Transformation resulted in cells that were significantly shorter than the nontransformed cells. The transformed strains also produced significantly more IAA than the nontransformed strains. Introduction of rpoS or gacS from Pseudomonas fluorescens was associated with a reduction in the production of both antibiotics, 2,4-diacetylphloroglucinol and mono-acetylphloroglucinol, produced by Enterobacter cloacae CAL2. With Enterobacter cloacae CAL2, plasmid-borne rpoS, but not gacS, increased the level of ACC deaminase activity, while introduction of rpoS in Enterobacter cloacae UW4 caused a decrease in ACC deaminase activity. Neither gacS nor rpoS significantly affected the level of siderophores synthesized by either bacterial strain. Overproduction of either GacA or RpoS in Enterobacter cloacae CAL2 resulted in a significant increase in the root lengths of canola seedlings when seeds were treated with the bacteria, and overproduction of RpoS caused an increase in canola shoot as well as root lengths.Key words: plant growth-promoting bacteria, canola, ethylene, ACC deaminase, GacS, RpoS, indoleacetic acid, siderophores, antibiotics.

scholarly journals Evaluation of Pseudomonas sp. for its multifarious plant growth promoting potential and its ability to alleviate biotic and abiotic stress in tomato (Solanum lycopersicum) plants

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Sangeeta Pandey ◽  
Shikha Gupta
Keyword(s):  
Plant Growth ◽  
Seed Treatment ◽  
Acc Deaminase ◽  
Hydrocyanic Acid ◽  
Rrna Gene ◽  
Pgp Traits ◽  
Tomato Plants ◽  
Biochemical Characterisation ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract1-Aminocyclopropane-1-carboxylate (ACC) deaminase activity is one of the most beneficial traits of plant growth promoting (PGP) rhizobacteria responsible for protecting the plants from detrimental effects of abiotic and biotic stress. The strain S3 with ACC deaminase activity (724.56 nmol α-ketobutyrate mg−1 protein hr−1) was isolated from rhizospheric soil of turmeric (Curcuma longa), a medicinal plant, growing in Motihari district of Indian state, Bihar. The halotolerant strain S3, exhibited optimum growth at 8% (w/v) NaCl. It also exhibited multiple PGP traits such as indole acetic acid production (37.71 μg mL−1), phosphate solubilization (69.68 mg L−1), siderophore, hydrocyanic acid (HCN) and ammonia production as well as revealed antagonism against Rhizoctonia solani. The potential of isolated strain to alleviate salinity stress in tomato plants was investigated through pots trials by inoculating strain S3 through-seed bacterization, soil drenching, root dipping as well as seed treatment + soil drenching. The strain S3 inoculated through seed treatment and soil drenching method led to improved morphological attributes (root/shoot length, root/shoot fresh weight and root/shoot dry weight), photosynthetic pigment content, increased accumulation of osmolytes (proline and total soluble sugar), enhanced activities of antioxidants (Catalase and Peroxidase) and phenolic content in salt stressed tomato plants. The biochemical characterisation, FAMEs analysis and 16S rRNA gene sequencing revealed that strain S3 belongs to the genus Pseudomonas. The overall findings of the study revealed that Pseudomonas sp. strain S3 can be explored as an effective plant growth promoter which stimulate growth and improve resilience in tomato plants under saline condition.

Determination of 1-aminocycopropane-1-carboxylic acid (ACC) to assess the effects of ACC deaminase-containing bacteria on roots of canola seedlings

2001 ◽  
Vol 47 (1) ◽  
pp. 77-80 ◽  
Author(s):  
Donna M Penrose ◽  
Barbara A Moffatt ◽  
Bernard R Glick
Keyword(s):  
Carboxylic Acid ◽  
Plant Growth ◽  
Root Elongation ◽  
Acc Deaminase ◽  
Plant Growth Promoting Bacteria ◽  
Psychrophilic Bacterium ◽  
Ethylene Inhibitor ◽  
Seedling Roots ◽  
Plant Growth Promoting ◽  
Growth Promoting

Previously, it was proposed that plant growth-promoting bacteria that possess the enzyme, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, can reduce the amount of ethylene produced by a plant and thereby promote root elongation. To test this model, canola seeds were imbibed in the presence of the chemical ethylene inhibitor, 2-aminoethoxyvinyl glycine (AVG), various strains of plant growth-promoting bacteria, and a psychrophilic bacterium containing an ACC deaminase gene on a broad host range plasmid. The extent of root elongation and levels of ACC, the immediate precursor of ethylene, were measured in the canola seedling roots. A modification of the Waters AccQ*Tag Amino Acid Analysis Method(tm) was used to quantify ACC in the root extracts. It was found that, in the presence of the ethylene inhibitor, AVG, or any one of several ACC deaminase-containing strains of bacteria, the growth of canola seedling roots was enhanced and the ACC levels in these roots were lowered.

scholarly journals BACTERIAL DIVERSITY WITH PLANT GROWTH-PROMOTING POTENTIAL ISOLATED FROM Agave americana RHIZOSPHERE

2021 ◽  
Author(s):  
CLARA IVETTE RINCON-MOLINA ◽  
FRANCISCO ALEXANDER RINCON-MOLINA ◽  
ADALBERTO ZENTENO-ROJAS ◽  
VICTOR MANUEL RUIZ-VALDIVIEZO ◽  
JOSE MIGUEL CULEBRO-RICALDI ◽  
...  
Keyword(s):  
Plant Growth ◽  
Rrna Gene ◽  
Plant Growth Promoting Bacteria ◽  
Rhizospheric Bacteria ◽  
Agave Americana ◽  
Plant Growth Promoting ◽  
Metagenomic Study ◽  
Growth Promoting ◽  
Native Strains ◽  
Potential Use

ABSTRACT Objective: Study the diversity of cultivable rhizospheric bacteria associated to Agave americana, and select native strains with potential as plant growth-promoting bacteria (PGPB). Design/methodology/approach: The isolated bacteria were phenotypically characterized. The genetic diversity and identity of the strains were revealed by genomic fingerprints and by sequencing of 16S rRNA gene. Plant growth promoting ability and plant inoculation assays were evaluated to know the potential as PGPB. Results: A total of 235 strains were isolated from A. americana rhizosphere and were classified within of 10 different bacterial genera. Rhizobium, Pseudomonas, Acinetobacter had high potential as PGPB. Study limitations/implications Cultivable approach was used to study rhizobacteria. A metagenomic study could expand the knowledge about the structure and diversity of bacterial community associated to A. americana. Findings/conclusions Rhizosphere bacteria have potential use as biofertilizer for the cultivation and propagation of A. americana and other agave species.

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