scholarly journals Effect of Pyroligneous Acid on the Microbial Community Composition and Plant Growth-Promoting Bacteria (PGPB) in Soils

Soil Systems ◽  
2022 ◽  
Vol 6 (1) ◽  
pp. 10
Author(s):  
Anithadevi Kenday Sivaram ◽  
Logeshwaran Panneerselvan ◽  
Kannappar Mukunthan ◽  
Mallavarapu Megharaj
Keyword(s):  
Microbial Community ◽  
Plant Growth ◽  
Microbial Diversity ◽  
Growth And Yield ◽  
Rrna Gene ◽  
Plant Growth Promoting Bacteria ◽  
Microbial Composition ◽  
Pyroligneous Acid ◽  
Plant Growth Promoting ◽  
Growth Promoting

Pyroligneous acid (PA) is often used in agriculture as a plant growth and yield enhancer. However, the influence of PA application on soil microorganisms is not often studied. Therefore, in this study, we investigated the effect of PA (0.01–5% w/w in soil) on the microbial diversity in two different soils. At the end of eight weeks of incubation, soil microbial community dynamics were determined by Illumina-MiSeq sequencing of 16S rRNA gene amplicons. The microbial composition differed between the lower (0.01% and 0.1%) and the higher (1% and 5%) concentration in both PA spiked soils. The lower concentration of PA resulted in higher microbial diversity and dehydrogenase activity (DHA) compared to the un-spiked control and the soil spiked with high PA concentrations. Interestingly, PA-induced plant growth-promoting bacterial (PGPB) genera include Bradyrhizobium, Azospirillum, Pseudomonas, Mesorhizobium, Rhizobium, Herbaspiriluum, Acetobacter, Beijerinckia, and Nitrosomonas at lower concentrations. Additionally, the PICRUSt functional analysis revealed the predominance of metabolism as the functional module’s primary component in both soils spiked with 0.01% and 0.1% PA. Overall, the results elucidated that PA application in soil at lower concentrations promoted soil DHA and microbial enrichment, particularly the PGPB genera, and thus have great implications for improving soil health.

Assessing the Plant Growth-promoting Traits and Host Specificity of Endophytic Bacteria of Pulse Crops

2021 ◽  
Author(s):  
R. Thamizh Vendan ◽  
D. Balachandar
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Growth And Yield ◽  
Rrna Gene ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Rrna Gene Sequencing ◽  
Plant Growth Promoting Traits

Background: Symbiotic associations between legumes and Rhizobia are ancient and fundamental. However, the plant growth-promoting endophytes other than Rhizobia are not yet fully explored for pulses productivity. The present study was aimed to isolate efficient endophytic bacteria from pulses, assess their diversity, screen their plant growth-promoting activities and to test their potential as bio inoculants for pulses.Methods: We have isolated several endophytic bacteria from pulse crops more specifically from blackgram (Vigna mungo) and greengram (Vigna radiata). After careful screening, 15 promising endophytic isolates were selected for this study. The identification of endophytic bacterial isolates was performed by 16S rRNA gene sequencing. The isolates were tested for their potential for the plant growth-promoting traits such as nitrogen fixation, phosphate solubilization, indole-3-acetic acid production, siderophore secretion and antifungal activity. Pot culture experiments were conducted with the screened potential endophytic cultures.Result: The 16S rRNA gene sequencing revealed that species of Enterobacter, Bacillus, Pantoea, Pseudomonas, Acromobacter, Ocrobacterium were found as endophytes in blackgram and greengram. The in vitro screening identified Bacillus pumilus (BG-E6), Pseudomonas fluorescens (BG-E5) and Bacillus licheniformis (BG-E3) from blackgram and Pseudomonas chlororaphis (GG-E2) and Bacillus thuringiensis (GG-E7) from greengram as potential plant growth-promoting endophytes. These strains showed antagonism against plant pathogenic fungi. Upon inoculation of these endophytic PGPR strains, the blackgram and greengram growth and yield got increased. Among the strains, BG-E6 recorded 14.7% increased yield in blackgram and GG-E2 accounted for a 19.5% yield increase in greengram compared to respective uninoculated control. The experimental results showed that there was a host specificity found among the endophytic bacterial cultures with pulses. The cross inoculation of endophytic strains did not perform well to enhance the growth and yield of their alternate hosts. 

scholarly journals Alone Yet Not Alone: Frankia Lives Under the Same Roof With Other Bacteria in Actinorhizal Nodules

2021 ◽  
Vol 12 ◽  
Author(s):  
Faten Ghodhbane-Gtari ◽  
Timothy D’Angelo ◽  
Abdellatif Gueddou ◽  
Sabrine Ghazouani ◽  
Maher Gtari ◽  
...  
Keyword(s):  
16S Rrna ◽  
Plant Growth ◽  
16S Rrna Gene ◽  
Root Nodules ◽  
Actinorhizal Plants ◽  
Rrna Gene ◽  
Plant Growth Promoting Bacteria ◽  
Metagenomic Sequencing ◽  
Plant Growth Promoting ◽  
Growth Promoting

Actinorhizal plants host mutualistic symbionts of the nitrogen-fixing actinobacterial genus Frankia within nodule structures formed on their roots. Several plant-growth-promoting bacteria have also been isolated from actinorhizal root nodules, but little is known about them. We were interested investigating the in planta microbial community composition of actinorhizal root nodules using culture-independent techniques. To address this knowledge gap, 16S rRNA gene amplicon and shotgun metagenomic sequencing was performed on DNA from the nodules of Casuarina glauca. DNA was extracted from C. glauca nodules collected in three different sampling sites in Tunisia, along a gradient of aridity ranging from humid to arid. Sequencing libraries were prepared using Illumina NextEra technology and the Illumina HiSeq 2500 platform. Genome bins extracted from the metagenome were taxonomically and functionally profiled. Community structure based off preliminary 16S rRNA gene amplicon data was analyzed via the QIIME pipeline. Reconstructed genomes were comprised of members of Frankia, Micromonospora, Bacillus, Paenibacillus, Phyllobacterium, and Afipia. Frankia dominated the nodule community at the humid sampling site, while the absolute and relative prevalence of Frankia decreased at the semi-arid and arid sampling locations. Actinorhizal plants harbor similar non-Frankia plant-growth-promoting-bacteria as legumes and other plants. The data suggests that the prevalence of Frankia in the nodule community is influenced by environmental factors, with being less abundant under more arid environments.

Effects of plant-growth-promoting bacteria on growth and yield of pepper under limited nitrogen supply

2009 ◽  
Vol 89 (2) ◽  
pp. 349-358 ◽  
Author(s):  
F. M. Del Amor ◽  
I. Porras
Keyword(s):  
Plant Growth ◽  
Capsicum Annuum ◽  
Growth And Yield ◽  
Plant Growth Promoting Bacteria ◽  
Total N ◽  
Capsicum Annuum L ◽  
N Supply ◽  
Fluorescence Maximum ◽  
Plant Growth Promoting ◽  
Growth Promoting

The influence of plant-growth-promoting bacteria (Azospirillum brasilense and Pantoea dispersa) on sweet pepper plants (Capsicum annuum L.) under limited N supply was studied. Inoculation did not affect leaf CO2 assimilation, Ci/Ca (the ratio of the intercellular to the ambient CO2 concentration), concentration of chlorophylls, chlorophyll fluorescence (maximum quantum efficiency of PSII) or SPAD readings. Total plant dry weight was significantly reduced in both inoculated and non-inoculated plants when the N supply was reduced from 12 (control) to 7 mM, whilst the NO3− and total-N concentrations in the leaves were not significantly affected by inoculation. Inoculation did not affect marketable fruit yield or the pigments (chlorophylls, lycopene and β-carotene) and carbohydrate (sucrose, glucose and fructose) contents in the fruits but flavonoids and anthocyanins were increased significantly by the addition of the bacteria, relative to non-inoculated plants under limited N supply. Key words: Plant-growth-promoting bacteria, Capsicum annuum L., soilless, photosynthesis, chlorophylls, flavonoids

scholarly journals Comparative Effects of Bio-Wastes in Combination with Plant Growth-Promoting Bacteria on Growth and Productivity of Okra

Agronomy ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2065
Author(s):  
Hammad Anwar ◽  
Xiukang Wang ◽  
Azhar Hussain ◽  
Muhammad Rafay ◽  
Maqshoof Ahmad ◽  
...  
Keyword(s):  
Plant Growth ◽  
Soil Health ◽  
Organic Amendments ◽  
Plant Growth Promoting Rhizobacteria ◽  
Fruit Weight ◽  
Growth And Yield ◽  
Plant Growth Promoting Bacteria ◽  
Randomized Design ◽  
Plant Growth Promoting ◽  
Growth Promoting

Plant growth-promoting rhizobacteria with multiple growth-promoting traits play a significant role in soil to improve soil health, crop growth and yield. Recent research studies have focused on the integration of organic amendments with plant growth-promoting rhizobacteria (PGPR) to enhance soil fertility and reduce the hazardous effects of chemical fertilizers. This study aims to evaluate the integrated application of biochar, compost, fruit and vegetable waste, and Bacillus subtilis (SMBL 1) to soil in sole application and in combined form. The study comprises eight treatments—four treatments without inoculation and four treatments with SMBL 1 inoculation in a completely randomized design (CRD), under factorial settings with four replications. The results indicate that the integrated treatments significantly improved okra growth and yield compared with sole applications. The integration of SMBL 1 with biochar showed significant improvements in plant height, root length, leaf chlorophyll a and b, leaf relative water content, fruit weight, diameter and length by 29, 29, 50, 53.3, 4.3, 44.7 and 40.4%, respectively, compared with control. Similarly, fruit N, P and K contents were improved by 33, 52.7 and 25.6% and Fe and Zn in shoot were 37.1 and 35.6%, respectively, compared with control. The results of this study reveal that the integration of SMBL 1 with organic amendments is an effective approach to the sustainable production of okra.

Sulfur-oxidizing buffalo dung bacteria enhance growth and yield of Foeniculum vulgare Mill.

2019 ◽  
Vol 65 (5) ◽  
pp. 377-386 ◽  
Author(s):  
Sandhya Dhiman ◽  
Ramesh Chand Dubey ◽  
Dinesh Kumar Maheshwari ◽  
Sandeep Kumar
Keyword(s):  
Plant Growth ◽  
Proteus Mirabilis ◽  
Growth And Yield ◽  
Rrna Gene ◽  
Bacterial Isolates ◽  
Foeniculum Vulgare ◽  
Plant Growth Promoting ◽  
Sulfur Oxidizing ◽  
Growth Promoting ◽  
Buffalo Dung

This study aimed to harness the benefits of sulfur-oxidizing beneficial bacteria from buffalo dung to improve crop yields of Foeniculum vulgare. A total of 61 bacterial isolates were screened from buffalo dung, of which 40 isolates exhibited plant-growth-promoting attributes, such as phosphate solubilization, indole-3-acetic acid production, and hydrogen cyanide production. Of these 40, four bacterial isolates, viz., BUFF12, BUFF14, BUFF23, and BUFF38, were the most potent, having plant-growth-promoting and sulfur-oxidizing properties. These four isolates produced phytase by solubilizing calcium phytate and sodium phytate. They solubilized potassium besides oxidizing the sulfur, causing an increase in soil fertility and crop production. All four isolates were nonpathogenic in nature, as demonstrated by a negative haemolysis test. According to the 16S rRNA gene sequence, the isolate BUFF14 was identified as Proteus mirabilis. Proteus mirabilis BUFF14 maximized seed germination with enhanced vegetative and reproductive parameters during pot and field trial studies, compared with the other isolates.

scholarly journals Allium cepa L. Inoculation with a Consortium of Plant Growth-Promoting Bacteria: Effects on Plant Growth and Development and Soil Fertility Status and Microbial Community

Proceedings ◽  
2021 ◽  
Vol 66 (1) ◽  
pp. 20
Author(s):  
Marika Pellegrini ◽  
Daniela M. Spera ◽  
Claudia Ercole ◽  
Maddalena del Gallo
Keyword(s):  
Microbial Community ◽  
Plant Growth ◽  
Soil Fertility ◽  
Allium Cepa ◽  
Bacterial Consortium ◽  
Plant Growth Promoting Bacteria ◽  
Allium Cepa L ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Molecular Profiles

The present work was aimed at investigating the effects of a four strains consortium—Azospirillum brasilense, Gluconacetobacter diazotrophicus, Herbaspirillum seropedicae, and Burkholderia ambifaria—on crops of Allium cepa L. and its soil health. The bacterial consortium was inoculated on seeds of two different onion varieties; inoculated seeds and control ones (treated with autoclaved inoculum) were sown in open-field and followed until harvest. Plant growth development parameters, as well as soil physico-chemical and molecular profiles (DNA extraction and 16S community sequencing on the Mi-Seq Illumina platform), were investigated. The results showed a positive influence of bacterial application on plant growth, with increased plant height (+18%), total chlorophylls (+42%), crop yields (+13%), and bulbs dry matter (+3%) than the control. The differences between control and treated experimental conditions were also underlined in the bulb extracts in terms of total phenolic contents (+25%) and antioxidant activities (+20%). Soil fertility and microbial community structure and diversity were also positively affected by the bacterial inoculum. At harvest, the soil with the presence of the bacterial consortium showed an increase of total organic carbon, organic matter, and available P and higher concentrations of nutrients than control. The ecological indexes calculated on the molecular profiles showed that community diversity was positively affected by the bacterial treatment. The present work allowed to remark the effective use of plant growth-promoting bacteria as a valid fertilization strategy to improve yield in productive landscapes, whilst safeguarding soil biodiversity.

scholarly journals BACTERIAL DIVERSITY WITH PLANT GROWTH-PROMOTING POTENTIAL ISOLATED FROM Agave americana RHIZOSPHERE

2021 ◽  
Author(s):  
CLARA IVETTE RINCON-MOLINA ◽  
FRANCISCO ALEXANDER RINCON-MOLINA ◽  
ADALBERTO ZENTENO-ROJAS ◽  
VICTOR MANUEL RUIZ-VALDIVIEZO ◽  
JOSE MIGUEL CULEBRO-RICALDI ◽  
...  
Keyword(s):  
Plant Growth ◽  
Rrna Gene ◽  
Plant Growth Promoting Bacteria ◽  
Rhizospheric Bacteria ◽  
Agave Americana ◽  
Plant Growth Promoting ◽  
Metagenomic Study ◽  
Growth Promoting ◽  
Native Strains ◽  
Potential Use

ABSTRACT Objective: Study the diversity of cultivable rhizospheric bacteria associated to Agave americana, and select native strains with potential as plant growth-promoting bacteria (PGPB). Design/methodology/approach: The isolated bacteria were phenotypically characterized. The genetic diversity and identity of the strains were revealed by genomic fingerprints and by sequencing of 16S rRNA gene. Plant growth promoting ability and plant inoculation assays were evaluated to know the potential as PGPB. Results: A total of 235 strains were isolated from A. americana rhizosphere and were classified within of 10 different bacterial genera. Rhizobium, Pseudomonas, Acinetobacter had high potential as PGPB. Study limitations/implications Cultivable approach was used to study rhizobacteria. A metagenomic study could expand the knowledge about the structure and diversity of bacterial community associated to A. americana. Findings/conclusions Rhizosphere bacteria have potential use as biofertilizer for the cultivation and propagation of A. americana and other agave species.

scholarly journals Screening of plant growth promoting bacteria associated with barley plants (Hordeum vulgare L.) cultivated in South Brazil

2015 ◽  
Vol 15 (2) ◽  
Author(s):  
Andress P. Pontes ◽  
Rocheli de Souza ◽  
Camille E. Granada ◽  
Luciane M.P. Passaglia
Keyword(s):  
Hordeum Vulgare ◽  
Plant Growth ◽  
Tricalcium Phosphate ◽  
Future Application ◽  
Rrna Gene ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Hordeum Vulgare L ◽  
Plant Growth Promoting ◽  
Growth Promoting

The occurrence of associations between bacteria and plant roots may be beneficial, neutral or detrimental. Plant growth promoting (PGP) bacteria form a heterogeneous group of beneficial microorganisms that can be found in the rhizosphere, the root surfaces or in association with host plant. The aim of this study was to isolate and characterize PGP bacteria associated to barley plants (Hordeum vulgare L.) aiming a future application as agricultural inoculant. One hundred and sixty bacterial strains were isolated from roots or rhizospheric soil of barley based on their growth in nitrogen-free selective media. They were evaluated for their ability to produce indolic compounds (ICs) and siderophores, and to solubilize tricalcium phosphate inin vitro assays. Most of them (74%) were able to synthesize ICs in the presence of the precursor L-tryptophan, while 57% of the isolates produced siderophores in Fe-limited liquid medium, and 17% were able to solubilize tricalcium phosphate. Thirty-two isolates possessing different PGP characteristics were identified by partial sequencing of their 16S rRNA gene. Strains belonging to Cedecea andMicrobacterium genera promoted the growth of barley plants in insoluble phosphate conditions, indicating that these bacteria could be used as bioinoculants contributing to decrease the amount of fertilizers applied in barley crops.

Sign in / Sign up

Export Citation Format

Share Document