scholarly journals Isolation of Francisella tularensis from Skin Ulcer after a Tick Bite, Austria, 2020

2021 ◽  
Vol 9 (7) ◽  
pp. 1407
Author(s):  
Mateusz Markowicz ◽  
Anna-Margarita Schötta ◽  
Freya Penatzer ◽  
Christoph Matscheko ◽  
Gerold Stanek ◽  
...  
Keyword(s):  
Lymph Node ◽  
Francisella Tularensis ◽  
Multilocus Sequence Typing ◽  
Skin Ulcer ◽  
Abscess Formation ◽  
Tick Bite ◽  
Tularensis Subsp ◽  
Human Host

Ulceroglandular tularemia is caused by the transmission of Francisella tularensis by arthropods to a human host. We report a case of tick-borne tularemia in Austria which was followed by an abscess formation in a lymph node, making drainage necessary. F. tularensis subsp. holarctica was identified by PCR and multilocus sequence typing.

scholarly journals Nanoaerosols reduce required effective dose of liposomal levofloxacin against pulmonary murine Francisella tularensis subsp. novicida infection

2016 ◽  
Vol 14 (1) ◽  
Author(s):  
Crystal N. Propst ◽  
Albert O. Nwabueze ◽  
Igor L. Kanev ◽  
Rachel E. Pepin ◽  
Bradford W. Gutting ◽  
...  
Keyword(s):  
Effective Dose ◽  
Francisella Tularensis ◽  
Tularensis Subsp

scholarly journals Susceptibility of 71 French isolates of Francisella tularensis subsp. holarctica to eight antibiotics and accuracy of the Etest® method

2008 ◽  
Vol 62 (1) ◽  
pp. 208-210 ◽  
Author(s):  
Eric Valade ◽  
Josée Vaissaire ◽  
Audrey Mérens ◽  
Eric Hernandez ◽  
Chantal Gros ◽  
...  
Keyword(s):  
Francisella Tularensis ◽  
Tularensis Subsp

Natural Infection of a European Red Squirrel (Sciurus vulgaris) with Francisella tularensis subsp. Holarctica

2021 ◽  
Vol 57 (4) ◽  
Author(s):  
Simone R. R. Pisano ◽  
Sonja Kittl ◽  
Ulrike Eulenberger ◽  
Joerg Jores ◽  
Francesco C. Origgi
Keyword(s):  
Francisella Tularensis ◽  
Natural Infection ◽  
Red Squirrel ◽  
Sciurus Vulgaris ◽  
Tularensis Subsp

scholarly journals Presence of Leishmania amastigotes in peritoneal fluid of a dog with leishmaniasis from Alagoas, Northeast Brazil

2006 ◽  
Vol 48 (4) ◽  
pp. 219-221 ◽  
Author(s):  
Filipe Dantas-Torres
Keyword(s):  
Bone Marrow ◽  
Lymph Node ◽  
Peritoneal Fluid ◽  
Skin Ulcer ◽  
Popliteal Lymph Node ◽  
Canine Leishmaniasis ◽  
Leishmania Infection ◽  
Intracellular Amastigotes ◽  
Skin Ulcers ◽  
Loss Of Weight

The goal of this short communication is to report the uncommon presence of intracellular amastigotes of Leishmania in peritoneal fluid of a dog with leishmaniasis from Alagoas State, Brazil. Physical examination of an adult male rottweiler suspected to be suffering of leishmaniasis revealed severe loss of weight, ascitis, splenomegaly, moderately enlarged lymph nodes, onychogryphosis, generalized alopecia, skin ulcers on the posterior limbs, and conjunctivitis. Samples of bone marrow, popliteal lymph node, skin ulcer, and peritoneal fluid were collected and smears of each sample were prepared and stained with hematoxylin and eosin. Numerous amastigotes were detected in bone marrow, popliteal lymph node, and skin ulcer smears. Smears of peritoneal fluid revealed the unusual presence of several free and intracellular amastigotes of Leishmania. Future studies are needed to determine whether the cytology of ascitic fluid represents a useful tool for diagnosis Leishmania infection in ascitic dogs, particularly in those living in areas where canine leishmaniasis is enzootic.

scholarly journals First European report of Francisella tularensis subsp. holarctica isolation from a domestic cat

2020 ◽  
Vol 51 (1) ◽  
Author(s):  
Sonja Kittl ◽  
Thierry Francey ◽  
Isabelle Brodard ◽  
Francesco C. Origgi ◽  
Stéphanie Borel ◽  
...  
Keyword(s):  
Francisella Tularensis ◽  
Domestic Cat ◽  
Tularensis Subsp

scholarly journals Contributions of Francisella tularensis subsp. novicida Chitinases and Sec Secretion System to Biofilm Formation on Chitin

2009 ◽  
Vol 76 (2) ◽  
pp. 596-608 ◽  
Author(s):  
Jeffrey J. Margolis ◽  
Sahar El-Etr ◽  
Lydia-Marie Joubert ◽  
Emily Moore ◽  
Richard Robison ◽  
...  
Keyword(s):  
Carbon Source ◽  
Francisella Tularensis ◽  
Biofilm Formation ◽  
Secreted Proteins ◽  
Tularensis Subsp ◽  
Transmission Mechanisms ◽  
One Step ◽  
Sec Translocon ◽  
Mammalian Infection ◽  
Exogenous Sugar

ABSTRACT Francisella tularensis, the zoonotic cause of tularemia, can infect numerous mammals and other eukaryotes. Although studying F. tularensis pathogenesis is essential to comprehending disease, mammalian infection is just one step in the ecology of Francisella species. F. tularensis has been isolated from aquatic environments and arthropod vectors, environments in which chitin could serve as a potential carbon source and as a surface for attachment and growth. We show that F. tularensis subsp. novicida forms biofilms during the colonization of chitin surfaces. The ability of F. tularensis to persist using chitin as a sole carbon source is dependent on chitinases, since mutants lacking chiA or chiB are attenuated for chitin colonization and biofilm formation in the absence of exogenous sugar. A genetic screen for biofilm mutants identified the Sec translocon export pathway and 14 secreted proteins. We show that these genes are important for initial attachment during biofilm formation. We generated defined deletion mutants by targeting two chaperone genes (secB1 and secB2) involved in Sec-dependent secretion and four genes that encode putative secreted proteins. All of the mutants were deficient in attachment to polystyrene and chitin surfaces and for biofilm formation compared to wild-type F. novicida. In contrast, mutations in the Sec translocon and secreted factors did not affect virulence. Our data suggest that biofilm formation by F. tularensis promotes persistence on chitin surfaces. Further study of the interaction of F. tularensis with the chitin microenvironment may provide insight into the environmental survival and transmission mechanisms of this pathogen.

scholarly journals Customizable PCR-Microplate Array for Differential Identification of Multiple Pathogens

2013 ◽  
Vol 76 (11) ◽  
pp. 1948-1957 ◽  
Author(s):  
ABDELA WOUBIT ◽  
TESHOME YEHUALAESHET ◽  
SHERRELLE ROBERTS ◽  
MARTHA GRAHAM ◽  
MOONIL KIM ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Francisella Tularensis ◽  
Yersinia Pseudotuberculosis ◽  
Salmonella Typhi ◽  
Rapid Identification ◽  
Shigella Dysenteriae ◽  
Tularensis Subsp ◽  
Differential Identification ◽  
Hot Dog ◽  
Simultaneous Identification

Customizable PCR-microplate arrays were developed for the rapid identification of Salmonella Typhimurium, Salmonella Saintpaul, Salmonella Typhi, Shigella dysenteriae, Escherichia coli O157:H7, Francisella tularensis subsp. tularensis, Francisella tularensis subsp. novicida, Vibrio cholerae, Vibrio parahaemolyticus, Yersinia pestis, and Yersinia pseudotuberculosis. Previously, we identified highly specific primers targeting each of these pathogens. Here, we report the development of customizable PCR-microplate arrays for simultaneous identification of the pathogens using the primers identified. A mixed aliquot of genomic DNA from 38 strains was used to validate three PCR-microplate array formats. Identical PCR conditions were used to run all the samples on the three formats. Specific amplifications were obtained on all three custom plates. In preliminary tests performed to evaluate the sensitivity of these assays in samples inoculated in the laboratory with Salmonella Typhimurium, amplifications were obtained from 1 g of beef hot dog inoculated at as low as 9 CFU/ml or from milk inoculated at as low as 78 CFU/ml. Such microplate arrays could be valuable tools for initial identification or secondary confirmation of contamination by these pathogens.

Borrelia garinii and Francisella tularensis subsp. holarctica detected in migratory shorebirds in Portugal

2012 ◽  
Vol 58 (5) ◽  
pp. 857-861 ◽  
Author(s):  
Isabel Lopes de Carvalho ◽  
Líbia Zé-Zé ◽  
Ana Sofia Alves ◽  
Sara Pardal ◽  
Ricardo Jorge Lopes ◽  
...  
Keyword(s):  
Francisella Tularensis ◽  
Borrelia Garinii ◽  
Tularensis Subsp ◽  
Migratory Shorebirds
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