scholarly journals Single-Cell Imaging for Studies of Renal Uranium Transport and Intracellular Behavior

Minerals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 191
Author(s):  
Shino Homma-Takeda ◽  
Hitomi Fujishiro ◽  
Izumi Tanaka ◽  
Haruko Yakumaru ◽  
Kyoko Ayama ◽  
...  

Nephrotoxicity is the primary health effect of uranium exposure. However, the renal transport and intracellular behavior of uranium remains to be clearly elucidated. In the present study, the intracellular uranium distribution was examined with the cell lines derived from the S3 segment of mouse renal proximal tubules, which is a toxic target site of uranium, using microbeam-based elemental analysis. Uranium exposure at 100 μM for 24 h (non-toxic phase) was performed in S3 cells. Two types of measurement specimens, including those that are adhesive cell specimens and cryosection specimens, were examined for the positional relationship of the intracellular localization of uranium. Based on the combined results of single-cell imaging from the two types of cell specimens, uranium was distributed inside the cell and localized in the cytoplasm near the cell nucleus. In some cells, uranium was colocalized with phosphorus and potassium. The amount of uranium accumulated in S3 cells was estimated using thin section-standards. The mean uranium content of three adhesive cells was hundreds of femtogram per cell. Thus, we believe that single-cell imaging would be useful for studies on renal uranium transportation and cellular behavior.

Author(s):  
UKM Teichgräber ◽  
JG Pinkernelle ◽  
F Neumann ◽  
T Benter ◽  
H Bruhn ◽  
...  

2018 ◽  
Vol 14 (2) ◽  
pp. 115-125 ◽  
Author(s):  
Andrea K. Pomerantz ◽  
Farid Sari-Sarraf ◽  
Kerri J. Grove ◽  
Liliana Pedro ◽  
Patrick J. Rudewicz ◽  
...  

Lab on a Chip ◽  
2011 ◽  
Vol 11 (1) ◽  
pp. 104-114 ◽  
Author(s):  
Min Jung Kim ◽  
Su Chul Lee ◽  
Sukdeb Pal ◽  
Eunyoung Han ◽  
Joon Myong Song

2019 ◽  
Vol 125 (Suppl_1) ◽  
Author(s):  
Yiqiang Zhang ◽  
Nuria Gago-Lopez ◽  
Ning Li ◽  
Zhenhe Zhang ◽  
Naima Alver ◽  
...  

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