scholarly journals Molecular Sex Identification in Dioecious Hippophae rhamnoides L. via RAPD and SCAR Markers

Molecules ◽  
2018 ◽  
Vol 23 (5) ◽  
pp. 1048 ◽  
Author(s):  
Wu Zhou ◽  
Yuwei Wang ◽  
Gong Zhang ◽  
Guangxiang Luan ◽  
Shasha Chen ◽  
...  
2012 ◽  
Vol 34 (5) ◽  
pp. 973-978 ◽  
Author(s):  
Girish Korekar ◽  
Ram Kumar Sharma ◽  
Rahul Kumar ◽  
Meenu ◽  
Naveen C. Bisht ◽  
...  

Author(s):  
Wu Zhou ◽  
Yuwei Wang ◽  
Gong Zhang ◽  
Guangxiang Luan ◽  
Shasha Chen ◽  
...  

The dioecious property of sea buckthorn (Hippophae rhamnoides L.) prevents sex recognition via traditional observation at the juvenile stage, thus impeding breeding and economic cropping; RAPD and SCAR markers were used to identify the sexes. A total of 45 random decamer primers were used to screen genomic DNA pools of staminate and pistillate genotypes for genetic polymorphisms. One female sex-linked marker was identified. D15 (5′-CATCCGTGCT-3′) amplified a particular band of 885 bp, which showed polymorphism among staminate and pistillate genotype plants. The SCAR marker Hrcx-15 was obtained by sequencing the fragment. The alleles of 140 pistillate genotypes were examined but not of the 140 staminate genotypes discerned via taxonomy. Staminate and pistillate genotypes of sea buckthorn plants can be distinguished, using Hrcx-15 as a genetic marker for sex identification and for expediting cultivation for commercial applications.


Author(s):  
О. М. Бедарева ◽  
В. Г. Сильвандер ◽  
Любовь Семёновна Мурачёва ◽  
А. В. Матюха

В статье рассмотрены наиболее крупные площади популяций облепихи (Hippophae rhamnoides L.) на территории районов Калининградской области. Отмечена уникальность облепиховых фитоценозов для региона. Рекомендованы природоохранные мероприятия, направленные на сохранение вида.


Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 847
Author(s):  
Anita Zapałowska ◽  
Natalia Matłok ◽  
Miłosz Zardzewiały ◽  
Tomasz Piechowiak ◽  
Maciej Balawejder

The aim of this research was to show the effect of the ozonation process on the quality of sea buckthorn (Hippophae rhamnoides L.). The quality of the ozonated berries of sea buckthorn was assessed. Prior to and after the ozone treatment, a number of parameters, including the mechanical properties, moisture content, microbial load, content of bioactive compounds, and composition of volatile compounds, were determined. The influence of the ozonation process on the composition of volatile compounds and mechanical properties was demonstrated. The ozonation had negligible impact on the weight and moisture of the samples immediately following the treatment. Significant differences in water content were recorded after 7 days of storage. It was shown that the highest dose of ozone (concentration and process time) amounting to 100 ppm for 30 min significantly reduced the water loss. The microbiological analyses showed the effect of ozone on the total count of aerobic bacteria, yeast, and mold. The applied process conditions resulted in the reduction of the number of aerobic bacteria colonies by 3 log cfu g−1 compared to the control (non-ozonated) sample, whereas the number of yeast and mold colonies decreased by 1 log cfu g−1 after the application of 100 ppm ozone gas for 30 min. As a consequence, ozone treatment enhanced the plant quality and extended plant’s storage life.


2018 ◽  
Vol 66 (3) ◽  
pp. 613-620 ◽  
Author(s):  
Jussi Suvanto ◽  
Petri Tähtinen ◽  
Saku Valkamaa ◽  
Marica T. Engström ◽  
Maarit Karonen ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document