Chromobacterium violaceum and Pseudomonas aeruginosa PAO1: Models for Evaluating Anti-Quorum Sensing Activity of Melaleuca alternifolia Essential Oil and Its Main Component Terpinen-4-ol

The problem of antibiotic resistance among pathogens encourages searching for novel active molecules. The aim of the research was to assay the anti-quorum sensing (anti-QS) and antibiofilm potential of Melaleuca alternifolia essential oil and its main constituent, terpinen-4-ol, to prevent the infections due to methicillin-resistant Staphylococcus aureus strains as an alternate to antibiotics. The tea tree oil (TTO) was evaluated for its potential in inhibiting QS-dependent phenomena such as violacein production in Chromobacterium violaceum, swarming motility of Pseudomonas aeruginosa PAO1, and biofilm formation in MRSA strains on glass. The results showed that terpinen-4-ol was able to inhibit MRSA strain biofilm formation on the glass strips by 73.70%. TTO inhibited the violacein production at a mean inhibitory concentration (MIC) value of 0.048 mg/mL by 69.3%. At 100 µg/mL TTO and terpinen-4-ol exhibited inhibition in swarming motility of PAO1 by 33.33% and 25%, respectively. TTO revealed anti-QS and anti-biofilm activities at very low concentrations, but it could be further investigated for new molecules useful for the treatment of MRSA infections.

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Inhibitive Effect of Eugenol and Its Nanoemulsion on Quorum Sensing–Mediated Virulence Factors and Biofilm Formation by Pseudomonas aeruginosa

Journal of Food Protection ◽

10.4315/0362-028x.jfp-18-196 ◽

2019 ◽

Vol 82 (3) ◽

pp. 379-389 ◽

Cited By ~ 8

Author(s):

ZAIXIANG LOU ◽

KEKGABILE S. LETSIDIDI ◽

FUHAO YU ◽

ZEJUN PEI ◽

HONGXIN WANG ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Quorum Sensing ◽

Biofilm Formation ◽

Homoserine Lactone ◽

Expression Level ◽

Signal Molecules ◽

Acyl Homoserine Lactone ◽

Swarming Motility ◽

Violacein Production ◽

Virulence Factor Production

ABSTRACT The aim of the present study was to evaluate the quorum sensing (QS) inhibition potential of eugenol and eugenol nanoemulsion against QS-dependent virulence factor production and gene expression, as well as biofilm formation in Pseudomonas aeruginosa. In the current study, eugenol nanoemulsion at a sub-MIC of 0.2 mg/mL specifically inhibited about 50% of the QS-mediated violacein production in Chromobacterium violaceum, as well as the production of N-(3-oxododecanoyl)-l-homoserine lactone (3-oxo-C12-HSL) and C4-HSL N-acyl homoserine lactone signal molecules, pyocyanin, and swarming motility in P. aeruginosa. The inhibitive effect of eugenol and its nanoemulsion on the expression of the QS synthase genes was concentration dependent, displaying 65 and 52% expression level for lasI, respectively, and 61 and 45% expression level for rhlI, respectively, at a concentration of 0.2 mg/mL. In addition, the inhibitive effect of eugenol and its nanoemulsion on the expression of the rhlA gene responsible for the production of rhamnolipid was also concentration dependent, displaying 65 and 51% expression level for the rhlA gene, respectively, at a concentration of 0.2 mg/mL. Eugenol and its nanoemulsion also displayed 36 and 63% respective inhibition of biofilm formation by P. aeruginosa at the 0.2 mg/mL concentration. Therefore, the nanoemulsion could be used as a novel QS-based antibacterial and antibiofilm agent for the control of harmful bacteria.

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Comparative Study of Inhibitory Potential of Dietary Phytochemicals Against Quorum Sensing Activity of and Biofilm Formation by Chromobacterium violaceum 12472, and Swimming and Swarming Behaviour of Pseudomonas aeruginosa PAO1

Food Technology and Biotechnology ◽

10.17113/ftb.57.02.19.5823 ◽

2019 ◽

Vol 57 (2) ◽

pp. 212-221 ◽

Cited By ~ 2

Author(s):

Elif Burcu Bali ◽

Kübra Erkan Türkmen ◽

Demet Erdönmez ◽

Necdet Sağlam

Keyword(s):

Pseudomonas Aeruginosa ◽

Quorum Sensing ◽

Gallic Acid ◽

Biofilm Formation ◽

Chromobacterium Violaceum ◽

Dependent Manner ◽

Pseudomonas Aeruginosa Pao1 ◽

Biosensor System ◽

Dietary Phytochemicals ◽

Inhibitory Potential

Quorum sensing (QS) and biofilm formation are important mechanisms related to antibiotic resistance of many pathogens. Alternative treatments are needed to prevent recurrent or chronic infections caused by multi-resistant pathogens. Therefore, the aim of this study is to investigate and compare the inhibitory potential of the dietary phytochemicals: curcumin, quercetin, apigenin, pyrogallol, gallic acid and luteolin against QS of and biofilm formation by Chromobacterium violaceum ATCC 12472 and the swimming and swarming abilities of Pseudomonas aeruginosa PAO1. Anti-QS potential of the phytochemicals was evaluated qualitatively and quantitatively using C. violaceum via the disk diffusion assay based on violacein pigment inhibition at the subminimal inhibitory concentrations ranging from 46.87 to 750 µg/mL. The results of anti-QS and antibiofilm activities on C. violaceum demonstrated that all the phytochemicals except pyrogallol and gallic acid inhibited violacein production (from (11.0±0.1) to (88.2±0.1) %) in a concentration-dependent manner. In addition, the biofilm formation was also significantly inhibited (p<0.05) in the presence of all the phytochemicals ((1.38±0.08)–(84.2±0.2) %). In the present study, the results revealed that quercetin, curcumin, apigenin and luteolin could be promising QS and biofilm inhibitory agents against the C. violaceum 12472 biosensor system. Our findings also suggest that all the phytochemicals, especially curcumin, quercetin and pyrogallol, might be anti-pathogenic agents against P. aeruginosa PAO1 infections due to the ability to control QS. However, more comprehensive studies at the molecular level, explaining their anti-QS mechanisms, need to be conducted to confirm these results and identify the genes involved.

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Sclerocarya birrea (A. Rich.) Hochst. and Sterculia setigera Del. extracts as a potential inhibitor of Pseudomonas aeruginosa PAO1 and Chromobacterium violaceum CV026 virulence factors to combat bacterial pathogenicity

World Journal of Biology Pharmacy and Health Sciences ◽

10.30574/wjbphs.2021.5.1.0075 ◽

2021 ◽

Vol 5 (1) ◽

pp. 009-018

Author(s):

Dramane Paré ◽

Jotham Yhi-pênê N’do ◽

Vincent Ouédraogo ◽

Abdel Magid Arsène Ouoba ◽

Adama Hilou

Keyword(s):

Pseudomonas Aeruginosa ◽

Ascorbic Acid ◽

Quorum Sensing ◽

Biofilm Formation ◽

Antimicrobial Agents ◽

Negative Control ◽

Chromobacterium Violaceum ◽

Bacterial Strains ◽

Phytochemical Study ◽

Pseudomonas Aeruginosa Pao1

The abuse of antibiotics has led to the widespread development of resistant bacterial strains, there is a need for the development of antimicrobial agents with new properties. Aim of this study was to determine the anti-quorum sensing potential (expression of virulence factor inhibition) of ethanolic extracts of S. birrea and S. setigera on Chromobacterium violaceum CV026 and Pseudomonas aeruginosa PAO1. The ability of the extracts to inhibit the production of violacein by C. violaceum and the biofilm formation of Pseudomonas aeruginosa was examined to determine anti-quorum sensing activity. The phytochemical screening and the determination of total polyphenols and total flavonoids were carried out. The antioxidant activity of the extracts was measured using anti-free radical DPPH model and the reducing of the ferric ion capacity. For the inhibition of the production of violacein, S. setigera presented the best result with an inhibition of 70.19% compared to the negative control which is 1% DMSO. This inhibition is greater than that of ascorbic acid used as a reference. This species was also the most active on biofilm formation with an inhibition of 72.24% compared to the negative control, it thus showed greater activity than that of ascorbic acid used as a positive reference. The phytochemical study revealed the presence of tannin, flavonoids, triterpenes, saponosides, coumarin in our two extracts. S. birrea gave the best total polyphenol content with 326.05 ± 117.90 mg EAG / g of extract and showed the best result on the DPPH radical with an inhibition of 73.33 ± 1.46% which is statistically equal to that of gallic acid used as a reference. These extracts showed good antibacterial activity by inhibiting the quorum sensing system. Thus, they could therefore be used for the manufacture of anti-quorum sensing phytomedicines without a bactericidal effect.

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Faculty Opinions recommendation of The impact of quorum sensing and swarming motility on Pseudomonas aeruginosa biofilm formation is nutritionally conditional.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1050540.502445 ◽

2006 ◽

Author(s):

Robert Palmer

Keyword(s):

Pseudomonas Aeruginosa ◽

Quorum Sensing ◽

Biofilm Formation ◽

Swarming Motility ◽

The Impact

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Peptide Mix from Olivancillaria hiatula Interferes with Cell-to-Cell Communication in Pseudomonas aeruginosa

BioMed Research International ◽

10.1155/2019/5313918 ◽

2019 ◽

Vol 2019 ◽

pp. 1-12

Author(s):

Edward Ntim Gasu ◽

Hubert Senanu Ahor ◽

Lawrence Sheringham Borquaye

Keyword(s):

Pseudomonas Aeruginosa ◽

Quorum Sensing ◽

Biofilm Formation ◽

Antimicrobial Agents ◽

Cell Communication ◽

Microtiter Plate ◽

Antibiofilm Activity ◽

Dependent Manner ◽

Biofilm Inhibition ◽

Swarming Motility

Bacteria in biofilms are encased in an extracellular polymeric matrix that limits exposure of microbial cells to lethal doses of antimicrobial agents, leading to resistance. In Pseudomonas aeruginosa, biofilm formation is regulated by cell-to-cell communication, called quorum sensing. Quorum sensing facilitates a variety of bacterial physiological functions such as swarming motility and protease, pyoverdine, and pyocyanin productions. Peptide mix from the marine mollusc, Olivancillaria hiatula, has been studied for its antibiofilm activity against Pseudomonas aeruginosa. Microscopy and microtiter plate-based assays were used to evaluate biofilm inhibitory activities. Effect of the peptide mix on quorum sensing-mediated processes was also evaluated. Peptide mix proved to be a good antibiofilm agent, requiring less than 39 μg/mL to inhibit 50% biofilm formation. Micrographs obtained confirmed biofilm inhibition at 1/2 MIC whereas 2.5 mg/mL was required to degrade preformed biofilm. There was a marked attenuation in quorum sensing-mediated phenotypes as well. At 1/2 MIC of peptide, the expression of pyocyanin, pyoverdine, and protease was inhibited by 60%, 72%, and 54%, respectively. Additionally, swarming motility was repressed by peptide in a dose-dependent manner. These results suggest that the peptide mix from Olivancillaria hiatula probably inhibits biofilm formation by interfering with cell-to-cell communication in Pseudomonas aeruginosa.

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