scholarly journals Layered Extraction and Adsorption Performance of Extracellular Polymeric Substances from Activated Sludge in the Enhanced Biological Phosphorus Removal Process

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3358 ◽  
Author(s):  
Daxue Li ◽  
Hailing Xi
Keyword(s):  
Activated Sludge ◽  
Functional Groups ◽  
Phosphorus Removal ◽  
Extracellular Polymeric Substances ◽  
Cation Exchange Resin ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Adsorption Performance ◽  
Biological Phosphorus

A large amount of phosphorus was found in the extracellular polymeric substances (EPS) of activated sludge used in enhanced biological phosphorus removal (EBPR), so the role of EPS and extracellular phosphorus in EBPR should not be neglected. The composition and properties of tightly bound EPS (TB-EPS) and loosely bound EPS (LB-EPS) were significantly different, and it was necessary to study the adsorption performance of EPS through the fractionating of activated sludge into LB-EPS, TB-EPS and microbial cells. In this study, the adsorption performance of LB-EPS and TB-EPS for phosphate was explored by extracting LB-EPS and TB-EPS via sonication and cation exchange resin (CER), respectively. The results indicated that the sonication-CER method was an efficient and reliable extraction method for EPS with a synergistic effect. The performance of EPS in the adsorption/complexing of phosphate was excellent because of its abundant functional groups. Specifically, the type and content of metal elements and functional groups in TB-EPS were much greater than those in LB-EPS, which led to the key role of TB-EPS in the adsorption/complexing of phosphate. Finally, a metabolic model for EBPR with consideration of the adsorption performance of LB-EPS and TB-EPS was proposed.

A flux-based stoichiometric model of enhanced biological phosphorus removal metabolism

1998 ◽  
Vol 37 (4-5) ◽  
pp. 609-613
Author(s):  
J. Pramanik ◽  
P. L. Trelstad ◽  
J. D. Keasling
Keyword(s):  
Phosphorus Removal ◽  
Reaction Network ◽  
Bacterial Biomass ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Stoichiometric Model ◽  
Aerobic Reactor ◽  
Complete Set ◽  
Biological Phosphorus

Enhanced biological phosphorus removal (EBPR) in wastewater treatment involves metabolic cycling through the biopolymers polyphosphate (polyP), polyhydroxybutyrate (PHB), and glycogen. This cycling is induced through treatment systems that alternate between carbon-rich anaerobic and carbon-poor aerobic reactor basins. While the appearance and disappearance of these biopolymers has been documented, the intracellular pressures that regulate their synthesis and degradation are not well understood. Current models of the EBPR process have examined a limited number of metabolic pathways that are frequently lumped into an even smaller number of “reactions.” This work, on the other hand, uses a stoichiometric model that contains a complete set of the pathways involved in bacterial biomass synthesis and energy production to examine EBPR metabolism. Using the stoichiometric model we were able to analyze the role of EBPR metabolism within the larger context of total cellular metabolism, as well as predict the flux distribution of carbon and energy fluxes throughout the total reaction network. The model was able to predict the consumption of PHB, the degradation of polyP, the uptake of acetate and the release of Pi. It demonstrated the relationship between acetate uptake and Pi release, and the effect of pH on this relationship. The model also allowed analysis of growth metabolism with respect to EBPR.

Biochemical Aspects of Enhanced Biological Phosphorus Removal from Wastewater

1985 ◽  
Vol 17 (11-12) ◽  
pp. 23-41 ◽  
Author(s):  
M. C. Hascoet ◽  
M. Florentz ◽  
P. Granger
Keyword(s):  
Activated Sludge ◽  
Phosphorus Removal ◽  
Phosphorus Uptake ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
The Past ◽  
Nitrate Addition ◽  
The Subject ◽  
Biological Phosphorus ◽  
Microbiological Analyses

Enhanced biological phosphorus removal from wastewater by means of microorganisms found in activated sludge has for the past few years been the subject of much research and it is now commonly recognized that an activated sludge system must include alternating anaerobic-aerobic periods. The present article covers biochemical aspects of this phenomenon using a phosphorus removing biomass obtained in a laboratory-scale pilot with alternating phases and a synthetic substrate feed. The percentage of phosphorus obtained in the pilot sludge was four times greater than that of a conventional sludge plant. By exposing the same biomass to different conditions and using 31P Nuclear Magnetic Resonance, we were able to accurately pin-point the various forms of phosphorus found within cells and follow their development during the course of alternating phases. The following results were obtained:the transformation of phosphorus in its inorganic to polyphosphate form depends on the medium's level of oxygenation,the presence of nitrates disturbs the anaerobic period but does not affect phosphorus uptake in the aerated period.Continuous nitrate addition alters biomass behaviour in the anaerobic phase, which loses the capacity to release phosphorus,copper at a concentration of over 1 mg Cu2+/1 inhibits phosphorus uptake in the aerated phase. Various microbiological analyses made on the pilot biomass isolated conventional bacteria found in activated sludge.

Design of nutrient removal activated sludge systems

2003 ◽  
Vol 47 (11) ◽  
pp. 115-122 ◽  
Author(s):  
J. Manga ◽  
J. Ferrer ◽  
A. Seco ◽  
F. Garcia-Usach
Keyword(s):  
Mathematical Model ◽  
Activated Sludge ◽  
Nutrient Removal ◽  
Phosphorus Removal ◽  
Pilot Plant ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
User Friendly ◽  
Biological Phosphorus ◽  
Activated Sludge Systems

A mechanistic mathematical model for nutrient and organic matter removal was used to describe the behavior of a nitrification denitrification enhanced biological phosphorus removal (NDEBPR) system. This model was implemented in a user-friendly software DESASS (design and simulation of activated sludge systems). A 484-L pilot plant was operated to verify the model results. The pilot plant was operated for three years over three different sludge ages. The validity of the model was confirmed with data from the pilot plant. Also, the utility of DESASS as a valuable tool for designing NDEBPR systems was confirmed.

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