scholarly journals Development and Validation of QuEChERS Followed by UHPLC-ToF-MS Method for Determination of Multi-Mycotoxins in Pistachio Nuts

Molecules ◽  
2021 ◽  
Vol 26 (19) ◽  
pp. 5754
Author(s):  
Ana Rita Soares Mateus ◽  
Sílvia Barros ◽  
Angelina Pena ◽  
Ana Sanches Silva
Keyword(s):  
High Performance ◽  
Solid Phase ◽  
Quechers Method ◽  
Modified Silica ◽  
Modified Silica Gel ◽  
Flight Mass Spectrometry ◽  
High Lipid ◽  
Commission Regulation ◽  
Tof Ms

Pistachios are one of the types of tree nut fruits with the highest mycotoxin contamination, especially of aflatoxins, worldwide. This study developed a Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) method that was followed by Ultra-High Performance Liquid Chromatography combined with Time-of-Flight Mass Spectrometry (UHPLC–ToF-MS) for the determination of mycotoxins in pistachios. Different approaches to dispersive solid phase extraction as a clean-up method for high lipid matrices were evaluated. For this, classic sorbents such as C18 (octadecyl-modified silica) and PSA (primary secondary amine), and new classes of sorbents, namely EMR-Lipid (enhanced matrix removal-lipid) and Z-Sep (modified silica gel with zirconium oxide), were used. The QuEChERS method, followed by Z-Sep d-SPE clean-up, provided the best analytical performance for aflatoxins (AFB1, AFB2, AFG1 and AFG2), ochratoxin A (OTA), zearalenone (ZEA), toxin T2 (T2) and toxin HT-2 (HT2) in pistachios. The method was validated in terms of linearity, sensitivity, repeatability, interday precision and recovery; it achieved good results according to criteria imposed by Commission Regulation (EC) no. 401/2006. The method was applied to real samples and the results show that pistachios that are available in Portuguese markets are safe from mycotoxins that are of concern to human health.

Preparation of Imprinted Polymer with D-Phenylalanin on Silica Surface

2009 ◽  
Vol 419-420 ◽  
pp. 541-544
Author(s):  
Hong Xing Dong ◽  
Qiu Li Jiang ◽  
Fei Tong ◽  
Zhen Xing Wang ◽  
Jin Yong Tang
Keyword(s):  
Silica Gel ◽  
High Performance ◽  
Silica Surface ◽  
Solid Phase ◽  
Adsorption Property ◽  
Research Field ◽  
Imprinted Polymer ◽  
Modified Silica ◽  
Modified Silica Gel ◽  
Functional Monomers

Silica gel was modified with polymerizable double bonds on the surface. Then the molecular imprinting polymer imprinted with D-Phenylalanin was grafted on the surface of modified silica gel. The molecularly imprinted polymer (MIP) based on the surface of silica gel was characterized by IR and scanning electron microscopy (SEM). The adsorption property of D-Phenylalanin by MIP was mearsured. The MIP with the combined functional monomers exhibited better adsorption properties and selectivity compared with the corresponding non-imprinted polymers or MIP with the single functional monomer. The material could be used in separation research field, such as packing material of high performance liquid chromatography, solid-phase extraction and so on.

scholarly journals Purification, Identification, and Sensory Evaluation of Kokumi Peptides from Agaricus bisporus Mushroom

Foods ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 43 ◽  
Author(s):  
Tao Feng ◽  
Yang Wu ◽  
Zhiwen Zhang ◽  
Shiqing Song ◽  
Haining Zhuang ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Sensory Evaluation ◽  
Agaricus Bisporus ◽  
High Performance ◽  
Solid Phase ◽  
Gel Permeation Chromatography ◽  
Ultra Performance Liquid Chromatography ◽  
Taste Intensity ◽  
Flight Mass Spectrometry ◽  
Tof Ms

Agaricus bisporus can enhance the umami and salty taste in chicken soup, and also has a high protein content, which indicates that there might be some kokumi taste compounds in this mushroom. Therefore, through ultrafiltration, gel permeation chromatography (GPC), and reverse phase-high performance liquid chromatography (RP-HPLC), some peptides in fresh Agaricus bisporus mushroom were isolated. Then, these peptides were identified by sensory evaluation and ultra performance liquid chromatography (UPLC) coupled quadruple time of flight mass spectrometry (Q-TOF-MS). The sensory evaluation results showed that the addition of aqueous extract isolated from Agaricus bisporus to model chicken broth did enhance chicken broth’s complexity, mouthfulness, and palatability. UPLC-Q-TOF-MS analysis found that Gly-Leu-Pro-Asp (Mw = 399.99) and Gly-His-Gly-Asp (Mw = 383.99) might act as key molecules to cause kokumi taste. In order to verify the kokumi taste of the above two peptides, they were synthesized by solid-phase synthesis and the taste properties of these two peptides were further characterized by descriptive sensory evaluation and taste intensity tests. This work indicated that it was feasible to produce kokumi peptides from Agaricus bisporus.

scholarly journals Metabolite Profiling of Meridianin C In Vivo of Rat by UHPLC/Q-TOF MS

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Guozhe Zhang ◽  
Linxia Xiao ◽  
Liang Qi
Keyword(s):  
High Performance ◽  
Kinase Inhibitor ◽  
Solid Phase ◽  
Extraction Methods ◽  
Mass Spectral Fragmentation ◽  
Mass Spectral ◽  
Flight Mass Spectrometry ◽  
Fragmentation Patterns ◽  
Tof Ms

Meridianin C (MC), as a marine alkaloid, is a potent protein kinase inhibitor which exhibits good anticancer activity. However, the in vivo metabolism of MC has not been described to date. In this study, an ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF MS) method is employed to investigate the in vivo metabolites of MC in rats. Plasma, bile, urine, and feces are collected after a single oral dose of MC. Protein precipitation, solid phase extraction (SPE), and ultrasonic extraction methods are used to prepare samples. Based on the mass spectral fragmentation patterns, elution order, and retrieving literatures, a total of 13 metabolites of MC were detected and tentatively identified, utilizing MetaboLynx software. The metabolic pathways of MC in rats include N- or O-glucuronidation, O-sulfation, N-hydroxylation, dihydroxylation, and trihydroxylation. The relative content of the metabolites in each kinds of biological samples is also evaluated. This study will help to understand the in vivo properties of MC for the future usage.

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