Silica Hydride: A Separation Material Every Analyst Should Know About

This review describes the development, special features and applications of silica hydride-based stationary phases for HPLC. The unique surface of this material is in contrast to ordinary, standard silica, which is the material most frequently used in modern HPLC stationary phases. The standard silica surface contains mainly silanol (Si-OH) groups, while the silica hydride surface is instead composed of silicon-hydrogen groups, which is much more stable, less reactive and delivers different chromatographic and chemical characteristics. Other aspects of this material are described for each of the different bonded moieties available commercially. Some applications for each of these column types are also presented as well as a generic model for method development on silica hydride-based stationary phases.

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The Development of Silica Hydride Stationary Phases for High-Performance Liquid Chromatography from Conception to Commercialization

Separations ◽

10.3390/separations6020027 ◽

2019 ◽

Vol 6 (2) ◽

pp. 27 ◽

Cited By ~ 1

Author(s):

Maria Matyska ◽

Joseph Pesek

Keyword(s):

High Performance Liquid Chromatography ◽

Surface Modification ◽

Liquid Chromatography ◽

Stationary Phase ◽

High Performance ◽

Silica Surface ◽

Stationary Phases ◽

Retention Mechanism ◽

Silica Hydride ◽

Synthetic Approaches

The development of a stationary phase material for high-performance liquid chromatography based on a surface of silica hydride as opposed to silanols on ordinary silica is discussed including synthetic approaches, characterization, and applications. There are several synthetic approaches available to create a silica hydride surface. Modification of the Si–H moiety on the silica surface can be accomplished through the use of a hydrosilation reaction. Both the intermediate silica hydride and the material modified with an organic moiety can be characterized by a number of spectroscopic as well as a variety of other methods. Further insights into the retention mechanism are provided through chromatographic measurements. The ultimate utility of any chromatographic stationary phase material is determined by its success in solving challenging analytical problems. A broad range of applications is reviewed to illustrate the versatility and usefulness of silica hydride-based stationary phases.

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The Development of Silica Hydride Stationary Phases for High Performance Liquid Chromatography from Conception to Commercialization

10.20944/preprints201902.0238.v1 ◽

2019 ◽

Author(s):

Maria Matyska ◽

Joseph Pesek

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Stationary Phase ◽

High Performance ◽

Silica Surface ◽

Stationary Phases ◽

Retention Mechanism ◽

Organic Moiety ◽

Silica Hydride ◽

Synthetic Approaches

The development of a stationary phase material for high performance liquid chromatography based on a surface of silica hydride as opposed to silanols on ordinary silica is discussed including synthetic approaches, characterization and applications.  There are several synthetic approaches available to create a silica hydride surface.  Modification of the Si-H moiety on the silica surface can be accomplished through use of a hydrosilation reaction.  Both the intermediate silica hydride and the material modified with an organic moiety can be characterized by a number of spectroscopic as well as a variety of other methods.  Further insights into the retention mechanism are provide through chromatographic measurements.  The ultimate utility of any chromatographic stationary phase material is determined by its success for solving challenging analytical problems.  A broad range of applications are reviewed to illustrate the versatility and usefulness of silica hydride-based stationary phases. 

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Evaluation of Pirkle Stationary Phases in Chiral Method Development Screening for Compounds of Pharmaceutical Interest

Current Pharmaceutical Analysis ◽

10.2174/157341209787314918 ◽

2009 ◽

Vol 5 (1) ◽

pp. 10-20 ◽

Cited By ~ 6

Author(s):

William Holzheuer ◽

Megan Wong ◽

Gregory Webster

Keyword(s):

Method Development ◽

Stationary Phases

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Method Development and Optimization of Enantioseparations Using Macrocyclic Glycopeptide Chiral Stationary Phases

Chiral Separation Techniques ◽

10.1002/9783527611737.ch1 ◽

2007 ◽

pp. 1-28 ◽

Cited By ~ 1

Author(s):

Thomas E. Beesley ◽

J. T. Lee

Keyword(s):

Method Development ◽

Stationary Phases ◽

Chiral Stationary Phases ◽

Macrocyclic Glycopeptide

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Analytical and preparative separation and isolation of functionalized fullerenes by conventional HPLC stationary phases: method development and column screening

RSC Advances ◽

10.1039/d0ra02814b ◽

2020 ◽

Vol 10 (33) ◽

pp. 19211-19218

Author(s):

Merve Ergun Dönmez ◽

Helena Grennberg

Keyword(s):

Method Development ◽

Stationary Phases ◽

Hplc Method ◽

Complex Reaction ◽

Preparative Separation ◽

Isolation And Purification ◽

Functionalized Fullerenes

Isolation and purification of functionalized fullerenes from often complex reaction mixtures is challenging. Here, a simple and efficient HPLC method is presented.

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