scholarly journals Surface Stabilization Affects Toxicity of Silver Nanoparticles in Human Peripheral Blood Mononuclear Cells

Nanomaterials ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1390
Author(s):  
Barbara Vuković ◽  
Marija Milić ◽  
Blaženka Dobrošević ◽  
Mirta Milić ◽  
Krunoslav Ilić ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cell Death ◽  
Mitochondrial Membrane ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Human Immune System ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Human Immune ◽  
Positively Charged

Silver nanoparticles (AgNPs) are one of the most investigated metal-based nanomaterials. Their biocidal activity boosted their application in both diagnostic and therapeutic medical systems. It is therefore crucial to provide sound evidences for human-related safety of AgNPs. This study aimed to enhance scientific knowledge with regard to biomedical safety of AgNPs by investigating how their different surface properties affect human immune system. Methods: preparation, characterization and stability evaluation was performed for four differently coated AgNPs encompassing neutral, positive and negative agents used for their surface stabilization. Safety aspects were evaluated by testing interaction of AgNPs with fresh human peripheral blood mononuclear cells (hPBMC) by means of particle cellular uptake and their ability to trigger cell death, apoptosis and DNA damages through induction of oxidative stress and damages of mitochondrial membrane. Results: all tested AgNPs altered morphology of freshly isolated hPBMC inducing apoptosis and cell death in a dose- and time-dependent manner. Highest toxicity was observed for positively-charged and protein-coated AgNPs. Cellular uptake of AgNPs was also dose-dependently increased and highest for positively charged AgNPs. Intracellularly, AgNPs induced production of reactive oxygen species (ROS) and damaged mitochondrial membrane. Depending on the dose, all AgNPs exhibited genotoxic potential. Conclusions: this study provides systematic and comprehensive data showing how differently functionalized AgNPs may affect the human immune system. Presented results are a valuable scientific contribution to safety assessment of nanosilver-based blood-contacting medical products.

Mass Cytometry and Single‐Cell RNA‐seq Profiling of the Heterogeneity in Human Peripheral Blood Mononuclear Cells Interacting with Silver Nanoparticles

Small ◽  
2020 ◽  
Vol 16 (21) ◽  
pp. 1907674 ◽  
Author(s):  
My Kieu Ha ◽  
Sook Jin Kwon ◽  
Jang‐Sik Choi ◽  
Nguyen Thanh Nguyen ◽  
Jaewoo Song ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Single Cell ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Rna Seq ◽  
Mass Cytometry ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

scholarly journals Characterization, bio-uptake and toxicity of polymer-coated silver nanoparticles and their interaction with human peripheral blood mononuclear cells

2021 ◽  
Vol 12 ◽  
pp. 282-294
Author(s):  
Sahar Pourhoseini ◽  
Reilly T Enos ◽  
Angela E Murphy ◽  
Bo Cai ◽  
Jamie R Lead
Keyword(s):  
Silver Nanoparticles ◽  
Exposure Time ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Extensive Study ◽  
Peripheral Blood Mononuclear ◽  
Rpmi Medium ◽  
Blood Mononuclear Cells

Silver nanoparticles (AgNPs) are widely used in medical applications due to their antibacterial and antiviral properties. Despite the extensive study of AgNPs, their toxicity and their effect on human health is poorly understood, as a result of issues such as poor control of NP properties and lack of proper characterization. The aim of this study was to investigate the combined characterization, bio-uptake, and toxicity of well-characterized polyvinylpyrrolidone (PVP)-coated AgNPs in exposure media during exposure time using primary human cells (peripheral blood mononuclear cells (PBMCs)). AgNPs were synthesized in-house and characterized using a multimethod approach. Results indicated the transformation of NPs in RPMI medium with a change in size and polydispersity over 24 h of exposure due to dissolution and reprecipitation. No aggregation of NPs was observed in the RPMI medium over the exposure time (24 h). A dose-dependent relationship between PBMC uptake and Ag concentration was detected for both AgNP and AgNO3 treatment. There was approximately a two-fold increase in cellular Ag uptake in the AgNO3 vs the NP treatment. Cytotoxicity, using LDH and MTS assays and based on exposure concentrations was not significantly different when comparing NPs and Ag ions. Based on differential uptake, AgNPs were more toxic after normalizing toxicity to the amount of cellular Ag uptake. Our data highlights the importance of correct synthesis, characterization, and study of transformations to obtain a better understanding of NP uptake and toxicity. Statistical analysis indicated that there might be an individual variability in response to NPs, although more research is required.

THRB expression is affected by miR-26a and miR-496, but age-associated decrease of the expression of this gene in human peripheral blood mononuclear cells is not dependent on these miRNAs

2014 ◽  
Author(s):  
Eliza Pawlik-Pachucka ◽  
Monika Budzinska ◽  
Anna Domaszewska-Szostek ◽  
Magdalena Owczarz ◽  
Magdalena Gewartowska ◽  
...  
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

Hyperglycemia Activates the CD27-CD70 Axis on Human Peripheral Blood Mononuclear Cells

2020 ◽  
pp. ascls.119.002089
Author(s):  
Claire Owaja ◽  
Evelyn Roback-Navarro ◽  
Naomi Iwai ◽  
Nadine Lerret
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

Immunomodulatory Effects of Lactic Acid Bacteria on Human Peripheral Blood Mononuclear Cells

2003 ◽  
Vol 15 (4) ◽  
Author(s):  
J. Michalkiewicz ◽  
M. Krotkiewski ◽  
L. Gackowska ◽  
M. Wyszomirska-Golda ◽  
A. Helmin-Basa ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Immunomodulatory Effects ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells
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